################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## Acc65I AccI AclI AcsI AflII AgeI AhdI AlwNI AocI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanII BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsgI BsiWI BsmI BspHI BspMI BsrDI BsrI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII CpoI DraI DraIII DrdI Eam1105I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HindIII HpaI KpnI MaeI MamI MfeI MflI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI Tsp509I Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AccIII 372 TCCGGA T^CCGGA Alw44I 172 GTGCAC G^TGCAC AlwI 539 GGATC GGATC(4/5) Aor51HI 452 AGCGCT AGC^GCT ApaLI 172 GTGCAC G^TGCAC AspHI 172 GTGCAC G[AT]GC[AT]^C AvaI 583 CCCGAG C^[CT]CG[GA]G BalI 212 TGGCCA TGG^CCA BbeI 479 GGCGCC GGCGC^C BbsI 577 GTCTTC GAAGAC(2/6) BmyI 172 GTGCAC G[AGT]GC[ACT]^C BpuAI 577 GTCTTC GAAGAC(2/6) BsaI 586 GAGACC GGTCTC(1/5) BseAI 372 TCCGGA T^CCGGA BsiHKAI 172 GTGCAC G[AT]GC[AT]^C BsmBI 593 GAGACG CGTCTC(1/5) BsmFI 81 GTCCC GGGAC(10/14) BsoBI 583 CCCGAG C^[CT]CG[GA]G Bsp1286I 172 GTGCAC G[AGT]GC[ACT]^C Bsp1407I 197 TGTACA T^GTACA BspDI 178 ATCGAT AT^CGAT BspEI 372 TCCGGA T^CCGGA BsrGI 197 TGTACA T^GTACA BssHII 412 GCGCGC G^CGCGC ClaI 178 ATCGAT AT^CGAT DpnII 540 GATC ^GATC EagI 19 CGGCCG C^GGCCG EarI 253 CTCTTC CTCTTC(1/4) EclXI 19 CGGCCG C^GGCCG Eco47III 452 AGCGCT AGC^GCT Eco52I 19 CGGCCG C^GGCCG EcoNI 564 CCTCATCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 434 CCATGG C^C[AT][AT]GG HincII 613 GTCAAC GT[CT]^[GA]AC HindII 613 GTCAAC GT[CT]^[GA]AC HinfI 235 GACTC G^A[ACGT]TC KasI 479 GGCGCC G^GCGCC Ksp632I 253 CTCTTC CTCTTC(1/4) KspI 62 CCGCGG CCGC^GG MboI 540 GATC ^GATC MluNI 212 TGGCCA TGG^CCA MroI 372 TCCGGA T^CCGGA MscI 212 TGGCCA TGG^CCA MseI 383 TTAA T^TAA NarI 479 GGCGCC GG^CGCC NcoI 434 CCATGG C^CATGG NdeII 540 GATC ^GATC PflMI 429 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 235 GACTC GAGTC(4/5) PshAI 388 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 62 CCGCGG CCGC^GG Sau3AI 540 GATC ^GATC SfaNI 131 GCATC GCATC(5/9) SfoI 479 GGCGCC GGC^GCC SspBI 197 TGTACA T^GTACA StyI 434 CCATGG C^C[AT][AT]GG Tru9I 383 TTAA T^TAA Tsp45I 497 GTGAC ^GT[GC]AC TspRI 264 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 429 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AatII 354 GACGTC GACGT^C 595 GACGTC AvaII 29 GGTCC G^G[AT]CC 633 GGACC BanI 58 GGCACC G^G[CT][GA]CC 479 GGCGCC BsaWI 372 TCCGGA [AT]^CCGG[AT] 589 ACCGGA BseRI 406 GAGGAG GAGGAG(10/8) 571 GAGGAG BsiEI 19 CGGCCG CG[GA][CT]^CG 100 CGGTCG BstXI 203 CCAGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 272 CCATCGTCCTGG DdeI 340 CTGAG C^T[ACGT]AG 547 CTCAG DsaI 62 CCGCGG C^C[GA][CT]GG 434 CCATGG EaeI 19 CGGCCG [CT]^GGCC[GA] 212 TGGCCA FauI 657 CCCGC CCCGC(4/6) 26 GCGGG HaeII 452 AGCGCT [GA]GCGC^[CT] 479 GGCGCC MaeIII 497 GTGAC ^GT[ACGT]AC 517 GTTAC MboII 254 TCTTC GAAGA(8/7) 578 TCTTC MslI 175 CACATCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 177 CATCGATGTG NaeI 619 GCCGGC GCC^GGC 674 GCCGGC NgoMIV 619 GCCGGC G^CCGGC 674 GCCGGC PpuMI 28 GGGTCCT [GA]G^G[AT]CC[CT] 632 AGGACCC TaqI 179 TCGA T^CGA 569 TCGA TthHB8I 179 TCGA T^CGA 569 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI BbvI BcnI BpmI BsaHI BsaJI BsiYI BslI BsmAI BspLU11I BsrBI BsrFI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HpaII HphI ItaI MaeII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV NspI RsaI Sau96I ScrFI TseI