Restriction map for
pFN21AE3996
(Circular) (Six-base) MAPSORT of: pFN21AE3996.seq Check: 3289 from: 1 to: 5407
pFN21AE3996 5407 bp
With 182 enzymes: SgfI *
March 28, 2012 11:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2363 2604 3217 278
Size: 53 83 186 936 827 241 613 2468
Fragments arranged by size:
2468 936 827 613 241 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2750 1937
Size: 813 4594
Acc65I G'GTAC_C
Cuts at: 2728 2728
Size: 5407
AclI AA'CG_TT
Cuts at: 1563 3534 3907 1563
Size: 1971 373 3063
Fragments arranged by size:
3063 1971 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2380 3470 4854 5122 1632
Size: 748 1090 1384 268 1917
Fragments arranged by size:
1917 1384 1090 748 268
AfeI AGC'GCT
Cuts at: 2459 2459
Size: 5407
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5210
AflIII A'CryG_T
Cuts at: 1203 2082 2199 2698 4306 5283 1203
Size: 879 117 499 1608 977 1327
Fragments arranged by size:
1608 1327 977 879 499 117
AgeI A'CCGG_T
Cuts at: 4238 4238
Size: 5407
AhdI GACnn_n'nnGTC
Cuts at: 4136 4136
Size: 5407
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5407
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2159 1184
Size: 975 4432
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2265 2297 2265
Size: 32 5375
AlwNI CAG_nnn'CTG
Cuts at: 3150 4722 3150
Size: 1572 3835
ApaLI G'TGCA_C
Cuts at: 2177 3464 4620 2177
Size: 1287 1156 2964
Fragments arranged by size:
2964 1287 1156
ApoI r'AATT_y
Cuts at: 1493 2716 2942 3094 5203 1493
Size: 1223 226 152 2109 1697
Fragments arranged by size:
2109 1697 1223 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3961 160
Size: 872 2929 1606
Fragments arranged by size:
2929 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5407
AvaI C'yCGr_G
Cuts at: 1870 1943 2588 2732 1870
Size: 73 645 144 4545
Fragments arranged by size:
4545 645 144 73
BamHI G'GATC_C
Cuts at: 2737 3104 2737
Size: 367 5040
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2063 2484 2728 4183 618
Size: 325 217 627 276 421 244 1455 1842
Fragments arranged by size:
1842 1455 627 421 325 276 244 217
BanII G_rGCy'C
Cuts at: 1391 2726 1391
Size: 1335 4072
BbeI G_GCGC'C
Cuts at: 2488 2488
Size: 5407
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2575 928
Size: 971 676 3760
Fragments arranged by size:
3760 971 676
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3598 3632 1903
Size: 34 50 34 1577 34 3678
Fragments arranged by size:
3678 1577 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3299 4509 3299
Size: 1210 4197
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5407
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4018 136
Size: 107 122 71 631 605 2346 1525
Fragments arranged by size:
2346 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5402 5402
Size: 5407
BlpI GC'TnA_GC
Cuts at: 2824 2824
Size: 5407
Bme1580I G_kGCm'C
Cuts at: 1790 2181 3468 4624 1790
Size: 391 1287 1156 2573
Fragments arranged by size:
2573 1287 1156 391
BmgBI CAC'GTC
Cuts at: 1318 2650 1318
Size: 1332 4075
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3138 4096 442
Size: 416 53 83 399 24 1721 958 1753
Fragments arranged by size:
1753 1721 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5407
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2227 2417 2474 4067 1239
Size: 988 190 57 1593 2579
Fragments arranged by size:
2579 1593 988 190 57
Bpu10I CC'TnA_GC
Cuts at: 2345 2345
Size: 5407
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3532 4397 4695 3532
Size: 865 298 4244
Fragments arranged by size:
4244 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2585 4070 882
Size: 617 1086 1485 2219
Fragments arranged by size:
2219 1485 1086 617
BsaAI yAC'GTr
Cuts at: 493 1204 3198 493
Size: 711 1994 2702
Fragments arranged by size:
2702 1994 711
BsaBI GATnn'nnATC
Cuts at: 5401 5401
Size: 5407
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2360 2485 2601 3214
Size: 53 83 186 936 827 125 116 613
Cuts at: 3214 3596 4231 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 827 635 613 382 186 125
116 83 53
BsaWI w'CCGG_w
Cuts at: 2377 2594 3840 4238 4512 4659 5070 2377
Size: 217 1246 398 274 147 411 2714
Fragments arranged by size:
2714 1246 411 398 274 217 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2225 2255 1178
Size: 30 165 30 822 30 4330
Fragments arranged by size:
4330 822 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2426 2591 1186
Size: 1240 165 4002
Fragments arranged by size:
4002 1240 165
BseYI C'CCAG_C
Cuts at: 2151 4610 5106 2151
Size: 2459 496 2452
Fragments arranged by size:
2459 2452 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4834
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2027 2108 2857 3618 4646 1540
Size: 275 186 26 81 749 761 1028 2301
Fragments arranged by size:
2301 1028 761 749 275 186 81 26
BsiHKAI G_wGCw'C
Cuts at: 2181 3468 3553 4624 2181
Size: 1287 85 1071 2964
Fragments arranged by size:
2964 1287 1071 85
BsmI GAATG_Cn'
Cuts at: 2918 3011 2918
Size: 93 5314
BsmBI CGTCTCn'nnnn_
Cuts at: 2592 2592
Size: 5407
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2181 2726 3468 3553 4624 1391
Size: 399 391 545 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 545 399 391 85
BspEI T'CCGG_A
Cuts at: 2377 5070 2377
Size: 2693 2714
BspHI T'CATG_A
Cuts at: 3296 5026 3296
Size: 1730 3677
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2762 844
Size: 1918 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2023 2031 2542 2665 3294 1394
Size: 429 200 8 511 123 629 3507
Fragments arranged by size:
3507 629 511 429 200 123 8
BsrDI GCAATG_nn'
Cuts at: 65 1248 3902 4076 65
Size: 1183 2654 174 1396
Fragments arranged by size:
2654 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2060 2624 2679 4051 4238 1700
Size: 110 250 564 55 1372 187 2869
Fragments arranged by size:
2869 1372 564 250 187 110 55
BsrGI T'GTAC_A
Cuts at: 96 2202 96
Size: 2106 3301
BssHII G'CGCG_C
Cuts at: 1925 2417 1925
Size: 492 4915
BssSI C'ACGA_G
Cuts at: 3467 4479 3467
Size: 1012 4395
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5407
BstBI TT'CG_AA
Cuts at: 4211 4211
Size: 5407
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5407
BstXI CCAn_nnnn'nTGG
Cuts at: 2215 2284 2215
Size: 69 5338
BstYI r'GATC_y
Cuts at: 1972 2737 3104 3489 3506 4958 5044 5056 5402
Size: 765 367 385 17 1452 86 12 346
Cuts at: 5402 1972
Size: 1977
Fragments arranged by size:
1977 1452 765 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2067 2439 513
Size: 552 1002 372 3481
Fragments arranged by size:
3481 1002 552 372
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4668
Fragments arranged by size:
4668 683 56
BtsI GCAGTG_nn'
Cuts at: 2923 3717 3737 2923
Size: 794 20 4593
Fragments arranged by size:
4593 794 20
ClaI AT'CG_AT
Cuts at: 1554 2184 1554
Size: 630 4777
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5372
DraI TTT'AAA
Cuts at: 2711 2711
Size: 5407
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4414 809
Size: 507 3098 1802
Fragments arranged by size:
3098 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2024 2217 2854
Size: 54 1425 50 275 18 194 193 637
Cuts at: 2854 3743 5372 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 637 275 194 193 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2024 2854 1537
Size: 275 212 830 4090
Fragments arranged by size:
4090 830 275 212
EarI CTCTTCn'nnn_
Cuts at: 1353 2264 3337 1353
Size: 911 1073 3423
Fragments arranged by size:
3423 1073 911
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3994 4368 4514 387
Size: 1064 138 2405 374 146 1280
Fragments arranged by size:
2405 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2227 2380 2417 2474 3470 4067 4854
Size: 393 595 153 37 57 996 597 787
Cuts at: 4854 5122 1239
Size: 268 1524
Fragments arranged by size:
1524 996 787 597 595 393 268 153
57 37
EcoNI CCTnn'n_nnAGG
Cuts at: 2573 2573
Size: 5407
EcoO109I rG'GnC_Cy
Cuts at: 2034 2566 2638 2034
Size: 532 72 4803
Fragments arranged by size:
4803 532 72
EcoRI G'AATT_C
Cuts at: 2716 3094 2716
Size: 378 5029
FspI TGC'GCA
Cuts at: 3913 3913
Size: 5407
HaeII r_GCGC'y
Cuts at: 1678 2461 2488 4554 1678
Size: 783 27 2066 2531
Fragments arranged by size:
2531 2066 783 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4048 4080 4122 4154 1373
Size: 32 135 32 2476 32 42 32 2626
Fragments arranged by size:
2626 2476 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2620 2751 2997 669
Size: 988 281 682 131 246 3079
Fragments arranged by size:
3079 988 682 281 246 131
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5407
HpaI GTT'AAC
Cuts at: 2997 2997
Size: 5407
KasI G'GCGC_C
Cuts at: 2484 2484
Size: 5407
KpnI G_GTAC'C
Cuts at: 2732 2732
Size: 5407
MfeI C'AATT_G
Cuts at: 3006 3006
Size: 5407
MluI A'CGCG_T
Cuts at: 5283 5283
Size: 5407
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4521 4705 1305
Size: 3216 184 2007
Fragments arranged by size:
3216 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2219 10
Size: 54 1425 343 387 3198
Fragments arranged by size:
3198 1425 387 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2184 2186 3365 3724 3883 518
Size: 648 1018 2 1179 359 159 2042
Fragments arranged by size:
2042 1179 1018 648 359 159 2
MspA1I CmG'CkG
Cuts at: 1606 2069 2482 2556 2823 3500 4648 4893 1606
Size: 463 413 74 267 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 463 413 267 245 74
NaeI GCC'GGC
Cuts at: 2626 2681 2626
Size: 55 5352
NarI GG'CG_CC
Cuts at: 2485 2485
Size: 5407
NcoI C'CATG_G
Cuts at: 513 1065 2439 513
Size: 552 1374 3481
Fragments arranged by size:
3481 1374 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5407
NgoMIV G'CCGG_C
Cuts at: 2624 2679 2624
Size: 55 5352
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5407
NotI GC'GGCC_GC
Cuts at: 2854 2854
Size: 5407
NspI r_CATG'y
Cuts at: 1132 1229 2086 2203 2702 2765 4310 1132
Size: 97 857 117 499 63 1545 2229
Fragments arranged by size:
2229 1545 857 499 117 97 63
PasI CC'CwG_GG
Cuts at: 1348 2131 1348
Size: 783 4624
PciI A'CATG_T
Cuts at: 2082 2199 2698 4306 2082
Size: 117 499 1608 3183
Fragments arranged by size:
3183 1608 499 117
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2440 1108
Size: 1332 4075
PfoI T'CCnGG_A
Cuts at: 2243 2283 2243
Size: 40 5367
PmeI GTTT'AAAC
Cuts at: 2711 2711
Size: 5407
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3476 3508 4989 5021 3476
Size: 32 1481 32 3862
Fragments arranged by size:
3862 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2034 2638 2034
Size: 604 4803
PshAI GACnn'nnGTC
Cuts at: 1613 2397 1613
Size: 784 4623
PsiI TTA'TAA
Cuts at: 2977 2977
Size: 5407
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2685 2717 2685
Size: 32 5375
PstI C_TGCA'G
Cuts at: 830 2759 830
Size: 1929 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5407
SacII CC_GC'GG
Cuts at: 2070 2070
Size: 5407
SalI G'TCGA_C
Cuts at: 1936 2749 1936
Size: 813 4594
SbfI CC_TGCA'GG
Cuts at: 2759 2759
Size: 5407
ScaI AGT'ACT
Cuts at: 3655 5367 3655
Size: 1712 3695
SfcI C'TryA_G
Cuts at: 826 1045 2755 3890 4571 4762 826
Size: 219 1710 1135 681 191 1471
Fragments arranged by size:
1710 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2486 2486
Size: 5407
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5407
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5407
SmaI CCC'GGG
Cuts at: 1872 2734 1872
Size: 862 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3511 4412 4674 820
Size: 197 926 1568 901 262 1553
Fragments arranged by size:
1568 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3198 493
Size: 2705 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5407
SphI G_CATG'C
Cuts at: 1132 2765 1132
Size: 1633 3774
SspI AAT'ATT
Cuts at: 5 52 3331 5
Size: 47 3279 2081
Fragments arranged by size:
3279 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5407
StyI C'CwwG_G
Cuts at: 513 1065 2439 2846 513
Size: 552 1374 407 3074
Fragments arranged by size:
3074 1374 552 407
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3434 3451 3604 3789 4234 325
Size: 1255 1854 17 153 185 445 1498
Fragments arranged by size:
1854 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2202 3653 5365
Size: 274 80 33 51 1443 225 1451 1712
Cuts at: 5365 96
Size: 138
Fragments arranged by size:
1712 1451 1443 274 225 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5367 5399 5367
Size: 32 5375
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5407
XbaI T'CTAG_A
Cuts at: 2743 2743
Size: 5407
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5407
XmaI C'CCGG_G
Cuts at: 1870 2732 1870
Size: 862 4545
XmnI GAAnn'nnTTC
Cuts at: 3536 3536
Size: 5407
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2361 2602 3215 276
Size: 53 83 186 936 827 241 613 2468
Fragments arranged by size:
2468 936 827 613 241 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI SacII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI NruI NsiI PacI PmlI PspOMI PspXI PvuII
RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI
XcmI