(Circular) (Six-base) MAPSORT of: pFN21AE3991.seq Check: 2832 from: 1 to: 5590 pFN21AE3991 5590 bp With 182 enzymes: SgfI * March 28, 2012 11:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3400 278 Size: 53 83 186 936 1864 2468 Fragments arranged by size: 2468 1864 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2421 2863 2933 1937 Size: 484 442 70 4594 Fragments arranged by size: 4594 484 442 70 Acc65I G'GTAC_C Cuts at: 2911 2911 Size: 5590 AclI AA'CG_TT Cuts at: 1563 3717 4090 1563 Size: 2154 373 3063 Fragments arranged by size: 3063 2154 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3653 5037 5305 1632 Size: 2021 1384 268 1917 Fragments arranged by size: 2021 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5393 AflIII A'CryG_T Cuts at: 1203 2630 4489 5466 1203 Size: 1427 1859 977 1327 Fragments arranged by size: 1859 1427 1327 977 AgeI A'CCGG_T Cuts at: 4421 4421 Size: 5590 AhdI GACnn_n'nnGTC Cuts at: 2040 4319 2040 Size: 2279 3311 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5590 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5590 AlwNI CAG_nnn'CTG Cuts at: 3333 4905 3333 Size: 1572 4018 ApaLI G'TGCA_C Cuts at: 3647 4803 3647 Size: 1156 4434 ApoI r'AATT_y Cuts at: 1493 2163 2514 2878 2899 3125 3277 5386 1493 Size: 670 351 364 21 226 152 2109 1697 Fragments arranged by size: 2109 1697 670 364 351 226 152 21 AseI AT'TA_AT Cuts at: 160 1032 4144 160 Size: 872 3112 1606 Fragments arranged by size: 3112 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5590 AvaI C'yCGr_G Cuts at: 1870 1943 2915 1870 Size: 73 972 4545 Fragments arranged by size: 4545 972 73 BamHI G'GATC_C Cuts at: 2920 3287 2920 Size: 367 5223 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2911 4366 618 Size: 325 217 627 1124 1455 1842 Fragments arranged by size: 1842 1455 1124 627 325 217 BanII G_rGCy'C Cuts at: 1391 2909 1391 Size: 1518 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2360 2710 928 Size: 971 461 350 3808 Fragments arranged by size: 3808 971 461 350 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3781 3815 1903 Size: 34 1844 34 3678 Fragments arranged by size: 3678 1844 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2231 3482 4692 2231 Size: 1251 1210 3129 Fragments arranged by size: 3129 1251 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5590 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4201 136 Size: 107 122 71 631 605 2529 1525 Fragments arranged by size: 2529 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5585 5585 Size: 5590 BlpI GC'TnA_GC Cuts at: 3007 3007 Size: 5590 Bme1580I G_kGCm'C Cuts at: 1790 3651 4807 1790 Size: 1861 1156 2573 Fragments arranged by size: 2573 1861 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5590 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2311 3321 4279 Size: 416 53 83 399 24 894 1010 958 Cuts at: 4279 442 Size: 1753 Fragments arranged by size: 1753 1010 958 894 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5590 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4250 1239 Size: 3011 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2333 3715 4580 4878 2333 Size: 1382 865 298 3045 Fragments arranged by size: 3045 1382 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4253 882 Size: 617 2754 2219 Fragments arranged by size: 2754 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3381 493 Size: 711 2177 2702 Fragments arranged by size: 2702 2177 711 BsaBI GATnn'nnATC Cuts at: 5584 5584 Size: 5590 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3397 3779 4414 275 Size: 53 83 186 936 1864 382 635 1451 Fragments arranged by size: 1864 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4023 4421 4695 4842 5253 4023 Size: 398 274 147 411 4360 Fragments arranged by size: 4360 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5365 Fragments arranged by size: 5365 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5590 BseYI C'CCAG_C Cuts at: 4793 5289 4793 Size: 496 5094 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5017 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3040 3801 4829 1540 Size: 275 186 1039 761 1028 2301 Fragments arranged by size: 2301 1039 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3651 3736 4807 3651 Size: 85 1071 4434 Fragments arranged by size: 4434 1071 85 BsmI GAATG_Cn' Cuts at: 3101 3194 3101 Size: 93 5497 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2909 3651 3736 4807 1391 Size: 399 1119 742 85 1071 2174 Fragments arranged by size: 2174 1119 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5253 5253 Size: 5590 BspHI T'CATG_A Cuts at: 3479 5209 3479 Size: 1730 3860 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2945 844 Size: 2101 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3477 1394 Size: 429 1654 3507 Fragments arranged by size: 3507 1654 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2214 4085 4259 65 Size: 1183 966 1871 174 1396 Fragments arranged by size: 1871 1396 1183 966 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4234 4421 1700 Size: 110 2424 187 2869 Fragments arranged by size: 2869 2424 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5590 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5590 BssSI C'ACGA_G Cuts at: 3650 4662 3650 Size: 1012 4578 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5590 BstBI TT'CG_AA Cuts at: 4394 4394 Size: 5590 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5590 BstYI r'GATC_y Cuts at: 1972 2758 2920 3287 3672 3689 5141 5227 5239 Size: 786 162 367 385 17 1452 86 12 Cuts at: 5239 5585 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 786 385 367 346 162 86 17 12 BstZ17I GTA'TAC Cuts at: 2422 2422 Size: 5590 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5038 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4851 Fragments arranged by size: 4851 683 56 BtsI GCAGTG_nn' Cuts at: 3106 3900 3920 3106 Size: 794 20 4776 Fragments arranged by size: 4776 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5590 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5555 DraI TTT'AAA Cuts at: 2894 2894 Size: 5590 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2479 4597 809 Size: 507 1163 2118 1802 Fragments arranged by size: 2118 1802 1163 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3037 3926 5555 Size: 54 1425 50 275 18 1207 889 1629 Cuts at: 5555 8 Size: 43 Fragments arranged by size: 1629 1425 1207 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3037 1537 Size: 275 1225 4090 Fragments arranged by size: 4090 1225 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3520 1353 Size: 2167 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4177 4551 4697 387 Size: 1064 138 2588 374 146 1280 Fragments arranged by size: 2588 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3653 4250 5037 5305 1239 Size: 393 2021 597 787 268 1524 Fragments arranged by size: 2021 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2899 3277 2899 Size: 378 5212 EcoRV GAT'ATC Cuts at: 2123 2123 Size: 5590 FspI TGC'GCA Cuts at: 4096 4096 Size: 5590 HaeII r_GCGC'y Cuts at: 1678 4737 1678 Size: 3059 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2026 2058 2778 2810 4231 Size: 32 135 32 454 32 720 32 1421 Cuts at: 4231 4263 4305 4337 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1421 720 454 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2934 3180 669 Size: 988 281 996 246 3079 Fragments arranged by size: 3079 996 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5590 HpaI GTT'AAC Cuts at: 3180 3180 Size: 5590 KpnI G_GTAC'C Cuts at: 2915 2915 Size: 5590 MfeI C'AATT_G Cuts at: 3189 3189 Size: 5590 MluI A'CGCG_T Cuts at: 5466 5466 Size: 5590 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4704 4888 1305 Size: 3399 184 2007 Fragments arranged by size: 3399 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3768 Fragments arranged by size: 3768 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2398 2619 3548 3907 4066 518 Size: 648 1232 221 929 359 159 2042 Fragments arranged by size: 2042 1232 929 648 359 221 159 MspA1I CmG'CkG Cuts at: 1606 2692 3006 3683 4831 5076 1606 Size: 1086 314 677 1148 245 2120 Fragments arranged by size: 2120 1148 1086 677 314 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5038 NdeI CA'TA_TG Cuts at: 387 387 Size: 5590 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5590 NotI GC'GGCC_GC Cuts at: 3037 3037 Size: 5590 NspI r_CATG'y Cuts at: 1132 1229 2634 2948 4493 1132 Size: 97 1405 314 1545 2229 Fragments arranged by size: 2229 1545 1405 314 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5590 PciI A'CATG_T Cuts at: 2630 4489 2630 Size: 1859 3731 PflMI CCAn_nnn'nTGG Cuts at: 1108 2441 1108 Size: 1333 4257 PmeI GTTT'AAAC Cuts at: 2894 2894 Size: 5590 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3659 3691 5172 5204 3659 Size: 32 1481 32 4045 Fragments arranged by size: 4045 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5590 PsiI TTA'TAA Cuts at: 3160 3160 Size: 5590 PstI C_TGCA'G Cuts at: 830 2942 830 Size: 2112 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5590 PvuII CAG'CTG Cuts at: 2692 2692 Size: 5590 SalI G'TCGA_C Cuts at: 1936 2932 1936 Size: 996 4594 SbfI CC_TGCA'GG Cuts at: 2942 2942 Size: 5590 ScaI AGT'ACT Cuts at: 2562 3838 5550 2562 Size: 1276 1712 2602 Fragments arranged by size: 2602 1712 1276 SfcI C'TryA_G Cuts at: 826 1045 2099 2938 4073 4754 4945 826 Size: 219 1054 839 1135 681 191 1471 Fragments arranged by size: 1471 1135 1054 839 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5590 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5590 SmaI CCC'GGG Cuts at: 1872 2917 1872 Size: 1045 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2348 3694 4595 4857 820 Size: 197 926 405 1346 901 262 1553 Fragments arranged by size: 1553 1346 926 901 405 262 197 SnaBI TAC'GTA Cuts at: 493 3381 493 Size: 2888 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5590 SphI G_CATG'C Cuts at: 1132 2948 1132 Size: 1816 3774 SspI AAT'ATT Cuts at: 5 52 2838 3514 5 Size: 47 2786 676 2081 Fragments arranged by size: 2786 2081 676 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5590 StyI C'CwwG_G Cuts at: 513 1065 2138 3029 513 Size: 552 1073 891 3074 Fragments arranged by size: 3074 1073 891 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3617 3634 3787 3972 4417 325 Size: 1255 2037 17 153 185 445 1498 Fragments arranged by size: 2037 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2298 2560 3836 Size: 274 80 33 51 1443 321 262 1276 Cuts at: 3836 5548 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1276 321 274 262 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5550 5582 5550 Size: 32 5558 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5590 XbaI T'CTAG_A Cuts at: 2926 2926 Size: 5590 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2632 2632 Size: 5590 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5590 XmaI C'CCGG_G Cuts at: 1870 2915 1870 Size: 1045 4545 XmnI GAAnn'nnTTC Cuts at: 2343 2839 3719 2343 Size: 496 880 4214 Fragments arranged by size: 4214 880 496 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3398 276 Size: 53 83 186 936 1864 2468 Fragments arranged by size: 2468 1864 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI