################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII BamHI BbrPI BcgI BciVI BclI BfrI BlpI Bpu1102I BsaAI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI HphI KpnI KspI MfeI MluI MroI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 69 enzymes) # ################################### AatII 868 GACGTC GACGT^C AccII 513 CGCG CG^CG AfaI 918 GTAC GT^AC AflIII 686 ACACGT A^C[GA][CT]GT AlwNI 951 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 948 CCTCAGG CC^T[ACGT]AGG ApaI 959 GGGCCC GGGCC^C Asp700I 1185 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 636 GTGCTC G[AT]GC[AT]^C AvaI 43 CCCGGG C^[CT]CG[GA]G AvrII 1087 CCTAGG C^CTAGG BalI 1062 TGGCCA TGG^CCA BanI 47 GGCGCC G^G[CT][GA]CC BbeI 47 GGCGCC GGCGC^C BbsI 255 GAAGAC GAAGAC(2/6) BbvCI 819 GCTGAGG CCTCAGC(-5/-2) BglI 925 GCCACGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1087 CCTAGG C^CTAGG BpuAI 255 GAAGAC GAAGAC(2/6) BsaBI 402 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiHKAI 636 GTGCTC G[AT]GC[AT]^C BsmBI 226 GAGACG CGTCTC(1/5) BsoBI 43 CCCGGG C^[CT]CG[GA]G BspMI 587 ACCTGC ACCTGC(4/8) BsrFI 808 ACCGGC [GA]^CCGG[CT] BstAPI 629 GCATGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1130 TTCGAA TT^CGAA BstUI 513 CGCG CG^CG Bsu36I 948 CCTCAGG CC^T[ACGT]AGG CfoI 48 GCGC GCG^C Cfr10I 808 ACCGGC [GA]^CCGG[CT] DrdI 862 GACAGTGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 530 CTCTTC CTCTTC(1/4) Eco81I 948 CCTCAGG CC^T[ACGT]AGG FauI 850 CCCGC CCCGC(4/6) HaeII 47 GGCGCC [GA]GCGC^[CT] HhaI 48 GCGC GCG^C HinP1I 48 GCGC G^CGC HincII 844 GTTGAC GT[CT]^[GA]AC HindII 844 GTTGAC GT[CT]^[GA]AC KasI 47 GGCGCC G^GCGCC Ksp632I 530 CTCTTC CTCTTC(1/4) MamI 402 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1062 TGGCCA TGG^CCA MscI 1062 TGGCCA TGG^CCA MseI 691 TTAA T^TAA MslI 454 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 513 CGCG CG^CG NarI 47 GGCGCC GG^CGCC NcoI 854 CCATGG C^CATGG NspI 629 GCATGC [GA]CATG^[CT] NspV 1130 TTCGAA TT^CGAA PflMI 1027 CCACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 265 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsaI 918 GTAC GT^AC SfoI 47 GGCGCC GGC^GCC SfuI 1130 TTCGAA TT^CGAA SgrAI 807 CACCGGCG C[GA]^CCGG[CT]G SmaI 43 CCCGGG CCC^GGG SphI 629 GCATGC GCATG^C TaqI 1131 TCGA T^CGA Tru9I 691 TTAA T^TAA Tsp509I 693 AATT ^AATT TthHB8I 1131 TCGA T^CGA Van91I 1027 CCACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 106 CCAGATGGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 43 CCCGGG C^CCGGG XmaI 43 CCCGGG C^CCGGG XmnI 1185 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcyI 47 GGCGCC G[GA]^CG[CT]C 868 GACGTC AlwI 156 GATCC GGATC(4/5) 953 GGATC BanII 427 GGGCTC G[GA]GC[CT]^C 959 GGGCCC BcnI 43 CCCGG CC^[GC]GG 44 CCGGG BsaHI 47 GGCGCC G[GA]^CG[CT]C 868 GACGTC BsaI 128 GGTCTC GGTCTC(1/5) 507 GGTCTC BseRI 946 CTCCTC GAGGAG(10/8) 172 GAGGAG BsgI 1014 CTGCAC GTGCAG(16/14) 418 GTGCAG BsrDI 981 CATTGC GCAATG(2/0) 1041 CATTGC BstF5I 183 CATCC GGATG(2/0) 858 GGATG DraIII 485 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG 1028 CACATGGTG EcoT14I 854 CCATGG C^C[AT][AT]GG 1087 CCTAGG FokI 183 CATCC GGATG(9/13) 858 GGATG Hin1I 47 GGCGCC G[GA]^CG[CT]C 868 GACGTC MboII 255 GAAGA GAAGA(8/7) 531 TCTTC NciI 43 CCCGG CC^[GC]GG 44 CCGGG PvuII 75 CAGCTG CAG^CTG 644 CAGCTG SfaNI 143 GCATC GCATC(5/9) 536 GCATC SmlI 37 CTCAAG C^T[CT][GA]AG 131 CTCAAG StyI 854 CCATGG C^C[AT][AT]GG 1087 CCTAGG TfiI 441 GAATC G^A[AT]TC 567 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AciI AluI AvaII BbvI BfaI BglII BmyI BpmI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII Fnu4HI HaeIII HapII HgaI HinfI HpaII ItaI MaeI MaeII MaeIII MboI MflI MnlI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI PpuMI PstI Sau3AI Sau96I ScrFI SfcI TseI Tsp45I TspRI XhoII