(Circular) (Six-base) MAPSORT of: pFN21AE3967.seq Check: 510 from: 1 to: 5917 pFN21AE3967 5917 bp With 182 enzymes: SgfI * March 28, 2012 11:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2601 2601 Size: 5917 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2877 3727 278 Size: 53 83 186 936 1341 850 2468 Fragments arranged by size: 2468 1341 936 850 186 83 53 AccI GT'mk_AC Cuts at: 1937 3260 1937 Size: 1323 4594 Acc65I G'GTAC_C Cuts at: 3238 3238 Size: 5917 AclI AA'CG_TT Cuts at: 1563 4044 4417 1563 Size: 2481 373 3063 Fragments arranged by size: 3063 2481 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2284 3131 3980 5364 5632 1632 Size: 652 847 849 1384 268 1917 Fragments arranged by size: 1917 1384 849 847 652 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5720 AflIII A'CryG_T Cuts at: 1203 2691 4816 5793 1203 Size: 1488 2125 977 1327 Fragments arranged by size: 2125 1488 1327 977 AgeI A'CCGG_T Cuts at: 4748 4748 Size: 5917 AhdI GACnn_n'nnGTC Cuts at: 4646 4646 Size: 5917 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5917 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5917 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2168 2200 2168 Size: 32 5885 AlwNI CAG_nnn'CTG Cuts at: 2961 3660 5232 2961 Size: 699 1572 3646 Fragments arranged by size: 3646 1572 699 ApaI G_GGCC'C Cuts at: 2968 2968 Size: 5917 ApaLI G'TGCA_C Cuts at: 3974 5130 3974 Size: 1156 4761 ApoI r'AATT_y Cuts at: 1493 3226 3452 3604 5713 1493 Size: 1733 226 152 2109 1697 Fragments arranged by size: 2109 1733 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4471 160 Size: 872 3439 1606 Fragments arranged by size: 3439 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5917 AvaI C'yCGr_G Cuts at: 1870 1943 2048 3242 1870 Size: 73 105 1194 4545 Fragments arranged by size: 4545 1194 105 73 AvrII C'CTAG_G Cuts at: 3092 3092 Size: 5917 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2775 2808 2775 Size: 33 5884 BamHI G'GATC_C Cuts at: 3247 3614 3247 Size: 367 5550 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2052 3238 4693 618 Size: 325 217 627 265 1186 1455 1842 Fragments arranged by size: 1842 1455 1186 627 325 265 217 BanII G_rGCy'C Cuts at: 1391 2436 2968 3236 1391 Size: 1045 532 268 4072 Fragments arranged by size: 4072 1045 532 268 BbeI G_GCGC'C Cuts at: 2056 2056 Size: 5917 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2267 928 Size: 971 368 4578 Fragments arranged by size: 4578 971 368 BbvCI CC'TCA_GC Cuts at: 2825 2825 Size: 5917 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4108 4142 1903 Size: 34 2171 34 3678 Fragments arranged by size: 3678 2171 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3809 5019 3809 Size: 1210 4707 BclI T'GATC_A Cuts at: 1548 1548 Size: 5917 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2936 4528 Size: 107 122 71 631 605 337 927 1592 Cuts at: 4528 136 Size: 1525 Fragments arranged by size: 1592 1525 927 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2412 2476 2894 5912 2412 Size: 64 418 3018 2417 Fragments arranged by size: 3018 2417 418 64 BlpI GC'TnA_GC Cuts at: 3334 3334 Size: 5917 Bme1580I G_kGCm'C Cuts at: 1790 2341 2361 2968 3978 5134 1790 Size: 551 20 607 1010 1156 2573 Fragments arranged by size: 2573 1156 1010 607 551 20 BmgBI CAC'GTC Cuts at: 1318 3073 1318 Size: 1755 4162 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2487 3648 4606 Size: 416 53 83 399 24 1070 1161 958 Cuts at: 4606 442 Size: 1753 Fragments arranged by size: 1753 1161 1070 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5917 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2113 2673 2895 4577 1239 Size: 874 560 222 1682 2579 Fragments arranged by size: 2579 1682 874 560 222 Bpu10I CC'TnA_GC Cuts at: 2825 2825 Size: 5917 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2027 2121 4042 4907 5205 2027 Size: 94 1921 865 298 2739 Fragments arranged by size: 2739 1921 865 298 94 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2139 2518 4580 882 Size: 617 640 379 2062 2219 Fragments arranged by size: 2219 2062 640 617 379 BsaAI yAC'GTr Cuts at: 493 1204 3708 493 Size: 711 2504 2702 Fragments arranged by size: 2702 2504 711 BsaBI GATnn'nnATC Cuts at: 2411 5911 2411 Size: 3500 2417 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2053 2874 3724 4106 Size: 53 83 186 936 520 821 850 382 Cuts at: 4106 4741 275 Size: 635 1451 Fragments arranged by size: 1451 936 850 821 635 520 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4350 4748 5022 5169 5580 4350 Size: 398 274 147 411 4687 Fragments arranged by size: 4687 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2168 2198 1178 Size: 30 165 30 765 30 4897 Fragments arranged by size: 4897 765 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2192 2942 1186 Size: 1006 750 4161 Fragments arranged by size: 4161 1006 750 BseYI C'CCAG_C Cuts at: 2321 5120 5616 2321 Size: 2799 496 2622 Fragments arranged by size: 2799 2622 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2444 3004 1163 Size: 573 708 560 4076 Fragments arranged by size: 4076 708 573 560 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3367 4128 5156 1540 Size: 275 186 1366 761 1028 2301 Fragments arranged by size: 2301 1366 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2645 3978 4063 5134 2645 Size: 1333 85 1071 3428 Fragments arranged by size: 3428 1333 1071 85 BsmI GAATG_Cn' Cuts at: 3428 3521 3428 Size: 93 5824 BsmBI CGTCTCn'nnnn_ Cuts at: 2225 2225 Size: 5917 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2341 2361 2436 2645 2968 3236 3978 Size: 399 551 20 75 209 323 268 742 Cuts at: 3978 4063 5134 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 551 399 323 268 209 85 75 20 BspEI T'CCGG_A Cuts at: 5580 5580 Size: 5917 BspHI T'CATG_A Cuts at: 3806 5536 3806 Size: 1730 4187 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2601 3272 844 Size: 1757 671 3489 Fragments arranged by size: 3489 1757 671 BsrBI CCG'CTC Cuts at: 1394 1823 3804 1394 Size: 429 1981 3507 Fragments arranged by size: 3507 1981 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2985 3045 4412 4586 65 Size: 1183 1737 60 1367 174 1396 Fragments arranged by size: 1737 1396 1367 1183 174 60 BsrFI r'CCGG_y Cuts at: 1700 1810 2813 4561 4748 1700 Size: 110 1003 1748 187 2869 Fragments arranged by size: 2869 1748 1003 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5917 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5917 BssSI C'ACGA_G Cuts at: 3977 4989 3977 Size: 1012 4905 BstAPI GCAn_nnn'nTGC Cuts at: 794 2640 794 Size: 1846 4071 BstBI TT'CG_AA Cuts at: 3136 4721 3136 Size: 1585 4332 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5917 BstYI r'GATC_y Cuts at: 1972 2412 2476 2894 2958 3247 3614 3999 4016 Size: 440 64 418 64 289 367 385 17 Cuts at: 4016 5468 5554 5566 5912 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 440 418 385 367 346 289 86 64 64 17 12 Bsu36I CC'TnA_GG Cuts at: 2954 2954 Size: 5917 BtgI C'CryG_G Cuts at: 513 1065 2004 2306 2859 2931 513 Size: 552 939 302 553 72 3499 Fragments arranged by size: 3499 939 553 552 302 72 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5178 Fragments arranged by size: 5178 683 56 BtsI GCAGTG_nn' Cuts at: 2143 2603 2731 3167 3433 4227 4247 2143 Size: 460 128 436 266 794 20 3813 Fragments arranged by size: 3813 794 460 436 266 128 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5917 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5882 DraI TTT'AAA Cuts at: 3221 3221 Size: 5917 DraIII CAC_nnn'GTG Cuts at: 2495 3038 2495 Size: 543 5374 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2873 4924 809 Size: 507 1557 2051 1802 Fragments arranged by size: 2051 1802 1557 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 2168 2455 Size: 54 1425 50 275 18 204 134 287 Cuts at: 2455 3067 3364 4253 5882 8 Size: 612 297 889 1629 43 Fragments arranged by size: 1629 1425 889 612 297 287 275 204 134 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3364 1537 Size: 275 1552 4090 Fragments arranged by size: 4090 1552 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2541 3847 1353 Size: 1188 1306 3423 Fragments arranged by size: 3423 1306 1188 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4504 4878 5024 387 Size: 1064 138 2915 374 146 1280 Fragments arranged by size: 2915 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2113 2284 2673 2895 3131 3980 4577 Size: 393 481 171 389 222 236 849 597 Cuts at: 4577 5364 5632 1239 Size: 787 268 1524 Fragments arranged by size: 1524 849 787 597 481 393 389 268 236 222 171 EcoO109I rG'GnC_Cy Cuts at: 2548 2685 2803 2965 3097 2548 Size: 137 118 162 132 5368 Fragments arranged by size: 5368 162 137 132 118 EcoRI G'AATT_C Cuts at: 3226 3604 3226 Size: 378 5539 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2683 2683 Size: 5917 FspI TGC'GCA Cuts at: 4423 4423 Size: 5917 HaeII r_GCGC'y Cuts at: 1678 2056 5064 1678 Size: 378 3008 2531 Fragments arranged by size: 3008 2531 378 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2060 2092 2133 2165 4558 Size: 32 135 32 488 32 41 32 2393 Cuts at: 4558 4590 4632 4664 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2393 488 135 42 41 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2851 3261 3507 669 Size: 988 281 913 410 246 3079 Fragments arranged by size: 3079 988 913 410 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5917 HpaI GTT'AAC Cuts at: 3507 3507 Size: 5917 KasI G'GCGC_C Cuts at: 2052 2052 Size: 5917 KpnI G_GTAC'C Cuts at: 3242 3242 Size: 5917 MfeI C'AATT_G Cuts at: 3516 3516 Size: 5917 MluI A'CGCG_T Cuts at: 5793 5793 Size: 5917 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2154 5031 5215 1305 Size: 849 2877 184 2007 Fragments arranged by size: 2877 2007 849 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3069 10 Size: 54 1425 343 1237 2858 Fragments arranged by size: 2858 1425 1237 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2463 3875 4234 4393 518 Size: 648 1297 1412 359 159 2042 Fragments arranged by size: 2042 1412 1297 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2026 2082 2651 3333 4010 5158 5403 1606 Size: 420 56 569 682 677 1148 245 2120 Fragments arranged by size: 2120 1148 682 677 569 420 245 56 NarI GG'CG_CC Cuts at: 2053 2053 Size: 5917 NcoI C'CATG_G Cuts at: 513 1065 2004 2859 513 Size: 552 939 855 3571 Fragments arranged by size: 3571 939 855 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5917 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5917 NotI GC'GGCC_GC Cuts at: 3364 3364 Size: 5917 NspI r_CATG'y Cuts at: 1132 1229 2638 3275 4820 1132 Size: 97 1409 637 1545 2229 Fragments arranged by size: 2229 1545 1409 637 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5917 PciI A'CATG_T Cuts at: 4816 4816 Size: 5917 PflMI CCAn_nnn'nTGG Cuts at: 1108 3038 1108 Size: 1930 3987 PmeI GTTT'AAAC Cuts at: 3221 3221 Size: 5917 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2168 2200 3986 4018 5499 5531 2168 Size: 32 1786 32 1481 32 2554 Fragments arranged by size: 2554 1786 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2548 2685 2803 3097 2548 Size: 137 118 294 5368 Fragments arranged by size: 5368 294 137 118 PshAI GACnn'nnGTC Cuts at: 1613 2274 1613 Size: 661 5256 PsiI TTA'TAA Cuts at: 3487 3487 Size: 5917 PspOMI G'GGCC_C Cuts at: 2964 2964 Size: 5917 PstI C_TGCA'G Cuts at: 830 2598 2633 2650 3269 830 Size: 1768 35 17 619 3478 Fragments arranged by size: 3478 1768 619 35 17 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5917 PvuII CAG'CTG Cuts at: 2082 2651 2082 Size: 569 5348 SalI G'TCGA_C Cuts at: 1936 3259 1936 Size: 1323 4594 SanDI GG'GwC_CC Cuts at: 2803 2803 Size: 5917 SbfI CC_TGCA'GG Cuts at: 3269 3269 Size: 5917 ScaI AGT'ACT Cuts at: 4165 5877 4165 Size: 1712 4205 SfcI C'TryA_G Cuts at: 826 1045 2594 2629 2646 3265 4400 5081 5272 Size: 219 1549 35 17 619 1135 681 191 Cuts at: 5272 826 Size: 1471 Fragments arranged by size: 1549 1471 1135 681 619 219 191 35 17 SfoI GGC'GCC Cuts at: 2054 2054 Size: 5917 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5917 SgrAI Cr'CCGG_yG Cuts at: 1700 2813 1700 Size: 1113 4804 SmaI CCC'GGG Cuts at: 1872 2050 3244 1872 Size: 178 1194 4545 Fragments arranged by size: 4545 1194 178 SmlI C'TyrA_G Cuts at: 820 1017 1943 2042 2136 4021 4922 5184 820 Size: 197 926 99 94 1885 901 262 1553 Fragments arranged by size: 1885 1553 926 901 262 197 99 94 SnaBI TAC'GTA Cuts at: 493 3708 493 Size: 3215 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5917 SphI G_CATG'C Cuts at: 1132 2638 3275 1132 Size: 1506 637 3774 Fragments arranged by size: 3774 1506 637 SrfI GCCC'GGGC Cuts at: 2050 2050 Size: 5917 SspI AAT'ATT Cuts at: 5 52 3841 5 Size: 47 3789 2081 Fragments arranged by size: 3789 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5917 StyI C'CwwG_G Cuts at: 513 1065 2004 2859 3092 3356 513 Size: 552 939 855 233 264 3074 Fragments arranged by size: 3074 939 855 552 264 233 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2335 2506 2653 2858 3944 3961 4114 Size: 1255 755 171 147 205 1086 17 153 Cuts at: 4114 4299 4744 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1086 755 445 205 185 171 153 147 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4163 5875 96 Size: 274 80 33 51 1443 2186 1712 138 Fragments arranged by size: 2186 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3013 3045 3159 3191 5877 5909 3013 Size: 32 114 32 2686 32 3021 Fragments arranged by size: 3021 2686 114 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5917 XbaI T'CTAG_A Cuts at: 3253 3253 Size: 5917 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2118 2118 Size: 5917 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5917 XmaI C'CCGG_G Cuts at: 1870 2048 3242 1870 Size: 178 1194 4545 Fragments arranged by size: 4545 1194 178 XmnI GAAnn'nnTTC Cuts at: 3194 4046 3194 Size: 852 5065 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2875 3725 276 Size: 53 83 186 936 1341 850 2468 Fragments arranged by size: 2468 1341 936 850 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BfrBI BplI BsiWI BstXI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SwaI