(Linear) (Six-base) MAPSORT of: KIEE3967.seq Check: 5880 from: 1 to: 1212 KIEE3967.orf With 182 enzymes: SgfI * March 22, 2012 17:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 596 1212 Size: 596 616 AatII G_ACGT'C Cuts at: 0 872 1212 Size: 872 340 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 279 1126 1212 Size: 279 847 86 Fragments arranged by size: 847 279 86 AflIII A'CryG_T Cuts at: 0 686 1212 Size: 686 526 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 163 195 1212 Size: 163 32 1017 Fragments arranged by size: 1017 163 32 AlwNI CAG_nnn'CTG Cuts at: 0 956 1212 Size: 956 256 ApaI G_GGCC'C Cuts at: 0 963 1212 Size: 963 249 AvaI C'yCGr_G Cuts at: 0 43 1212 Size: 43 1169 AvrII C'CTAG_G Cuts at: 0 1087 1212 Size: 1087 125 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 770 803 1212 Size: 770 33 409 Fragments arranged by size: 770 409 33 BanI G'GyrC_C Cuts at: 0 47 1212 Size: 47 1165 BanII G_rGCy'C Cuts at: 0 431 963 1212 Size: 431 532 249 Fragments arranged by size: 532 431 249 BbeI G_GCGC'C Cuts at: 0 51 1212 Size: 51 1161 BbsI GAAGACnn'nnnn_ Cuts at: 0 262 1212 Size: 262 950 BbvCI CC'TCA_GC Cuts at: 0 820 1212 Size: 820 392 BglI GCCn_nnn'nGGC Cuts at: 0 931 1212 Size: 931 281 BglII A'GATC_T Cuts at: 0 407 471 889 1212 Size: 407 64 418 323 Fragments arranged by size: 418 407 323 64 Bme1580I G_kGCm'C Cuts at: 0 336 356 963 1212 Size: 336 20 607 249 Fragments arranged by size: 607 336 249 20 BmgBI CAC'GTC Cuts at: 0 1068 1212 Size: 1068 144 BmrI ACTGGGnnnn_n' Cuts at: 0 482 1212 Size: 482 730 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 108 668 890 1212 Size: 108 560 222 322 Fragments arranged by size: 560 322 222 108 Bpu10I CC'TnA_GC Cuts at: 0 820 1212 Size: 820 392 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 22 116 1212 Size: 22 94 1096 Fragments arranged by size: 1096 94 22 BsaI GGTCTCn'nnnn_ Cuts at: 0 134 513 1212 Size: 134 379 699 Fragments arranged by size: 699 379 134 BsaBI GATnn'nnATC Cuts at: 0 406 1212 Size: 406 806 BsaHI Gr'CG_yC Cuts at: 0 48 869 1212 Size: 48 821 343 Fragments arranged by size: 821 343 48 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 163 193 1212 Size: 163 30 1019 Fragments arranged by size: 1019 163 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 187 937 1212 Size: 187 750 275 Fragments arranged by size: 750 275 187 BseYI C'CCAG_C Cuts at: 0 316 1212 Size: 316 896 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 439 999 1212 Size: 439 560 213 Fragments arranged by size: 560 439 213 BsiHKAI G_wGCw'C Cuts at: 0 640 1212 Size: 640 572 BsmBI CGTCTCn'nnnn_ Cuts at: 0 220 1212 Size: 220 992 Bsp1286I G_dGCh'C Cuts at: 0 336 356 431 640 963 1212 Size: 336 20 75 209 323 249 Fragments arranged by size: 336 323 249 209 75 20 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 596 1212 Size: 596 616 BsrDI GCAATG_nn' Cuts at: 0 980 1040 1212 Size: 980 60 172 Fragments arranged by size: 980 172 60 BsrFI r'CCGG_y Cuts at: 0 808 1212 Size: 808 404 BstAPI GCAn_nnn'nTGC Cuts at: 0 635 1212 Size: 635 577 BstBI TT'CG_AA Cuts at: 0 1131 1212 Size: 1131 81 BstYI r'GATC_y Cuts at: 0 407 471 889 953 1212 Size: 407 64 418 64 259 Fragments arranged by size: 418 407 259 64 64 Bsu36I CC'TnA_GG Cuts at: 0 949 1212 Size: 949 263 BtgI C'CryG_G Cuts at: 0 301 854 926 1212 Size: 301 553 72 286 Fragments arranged by size: 553 301 286 72 BtsI GCAGTG_nn' Cuts at: 0 138 598 726 1162 1212 Size: 138 460 128 436 50 Fragments arranged by size: 460 436 138 128 50 DraIII CAC_nnn'GTG Cuts at: 0 490 1033 1212 Size: 490 543 179 Fragments arranged by size: 543 490 179 DrdI GACnn_nn'nnGTC Cuts at: 0 868 1212 Size: 868 344 EaeI y'GGCC_r Cuts at: 0 29 163 450 1062 1212 Size: 29 134 287 612 150 Fragments arranged by size: 612 287 150 134 29 EarI CTCTTCn'nnn_ Cuts at: 0 536 1212 Size: 536 676 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 108 279 668 890 1126 1212 Size: 108 171 389 222 236 86 Fragments arranged by size: 389 236 222 171 108 86 EcoO109I rG'GnC_Cy Cuts at: 0 543 680 798 960 1092 1212 Size: 543 137 118 162 132 120 Fragments arranged by size: 543 162 137 132 120 118 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 678 1212 Size: 678 534 HaeII r_GCGC'y Cuts at: 0 51 1212 Size: 51 1161 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 55 87 128 160 1212 Size: 55 32 41 32 1052 Fragments arranged by size: 1052 55 41 32 32 HincII GTy'rAC Cuts at: 0 846 1212 Size: 846 366 KasI G'GCGC_C Cuts at: 0 47 1212 Size: 47 1165 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 149 1212 Size: 149 1063 MscI TGG'CCA Cuts at: 0 1064 1212 Size: 1064 148 MslI CAynn'nnrTG Cuts at: 0 458 1212 Size: 458 754 MspA1I CmG'CkG Cuts at: 0 21 77 646 1212 Size: 21 56 569 566 Fragments arranged by size: 569 566 56 21 NarI GG'CG_CC Cuts at: 0 48 1212 Size: 48 1164 NcoI C'CATG_G Cuts at: 0 854 1212 Size: 854 358 NspI r_CATG'y Cuts at: 0 633 1212 Size: 633 579 PflMI CCAn_nnn'nTGG Cuts at: 0 1033 1212 Size: 1033 179 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 163 195 1212 Size: 163 32 1017 Fragments arranged by size: 1017 163 32 PpuMI rG'GwC_Cy Cuts at: 0 543 680 798 1092 1212 Size: 543 137 118 294 120 Fragments arranged by size: 543 294 137 120 118 PshAI GACnn'nnGTC Cuts at: 0 269 1212 Size: 269 943 PspOMI G'GGCC_C Cuts at: 0 959 1212 Size: 959 253 PstI C_TGCA'G Cuts at: 0 593 628 645 1212 Size: 593 35 17 567 Fragments arranged by size: 593 567 35 17 PvuII CAG'CTG Cuts at: 0 77 646 1212 Size: 77 569 566 Fragments arranged by size: 569 566 77 SanDI GG'GwC_CC Cuts at: 0 798 1212 Size: 798 414 SfcI C'TryA_G Cuts at: 0 589 624 641 1212 Size: 589 35 17 571 Fragments arranged by size: 589 571 35 17 SfoI GGC'GCC Cuts at: 0 49 1212 Size: 49 1163 SgrAI Cr'CCGG_yG Cuts at: 0 808 1212 Size: 808 404 SmaI CCC'GGG Cuts at: 0 45 1212 Size: 45 1167 SmlI C'TyrA_G Cuts at: 0 37 131 1212 Size: 37 94 1081 Fragments arranged by size: 1081 94 37 SphI G_CATG'C Cuts at: 0 633 1212 Size: 633 579 SrfI GCCC'GGGC Cuts at: 0 45 1212 Size: 45 1167 StyI C'CwwG_G Cuts at: 0 854 1087 1212 Size: 854 233 125 Fragments arranged by size: 854 233 125 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 330 501 648 853 1212 Size: 330 171 147 205 359 Fragments arranged by size: 359 330 205 171 147 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1008 1040 1154 1186 1212 Size: 1008 32 114 32 26 Fragments arranged by size: 1008 114 32 32 26 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 113 1212 Size: 113 1099 XmaI C'CCGG_G Cuts at: 0 43 1212 Size: 43 1169 XmnI GAAnn'nnTTC Cuts at: 0 1189 1212 Size: 1189 23 ZraI GAC'GTC Cuts at: 0 870 1212 Size: 870 342 Enzymes that do cut: AarI AatII AcuI AflIII AloI AlwNI ApaI AvaI AvrII BaeI BanI BanII BbeI BbsI BbvCI BglI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI BstAPI BstBI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EarI Eco57MI EcoO109I FalI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NarI NcoI NspI PflMI PpiI PpuMI PshAI PspOMI PstI PvuII SanDI SfcI SfoI SgrAI SmaI SmlI SphI SrfI StyI TaqII TstI XcmI XmaI XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI ApaLI ApoI AscI AseI AsiSI BamHI BcgI BciVI BclI BfrBI BlpI BmtI BplI BsaAI BsaWI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrGI BssHII BssSI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SgfI SnaBI SpeI SspI StuI SwaI TatI Tth111I XbaI XhoI