################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AgeI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AvrII BamHI BbrPI BbvCI BciVI BclI BfaI BfrI BlnI BlpI Bpu1102I BsaBI BseAI BsmI BspEI BspHI BspLU11I BsrDI BssSI BstBI BstEII BstPI BstXI Bsu36I CelII CpoI DraI DraII DraIII Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HpaI KpnI MaeI MamI MfeI MluI MroI MseI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII ScaI SexAI SfiI SfuI SmaI SnaBI SpeI SspI SwaI Tru9I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 139 GACGTC GACGT^C AflIII 1412 ACGTGT A^C[GA][CT]GT AlwI 1130 GGATC GGATC(4/5) Asp700I 516 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 869 TGCGCA TGC^GCA BalI 1199 TGGCCA TGG^CCA BbsI 1186 GAAGAC GAAGAC(2/6) BcgI 1727 GCAGGGGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 380 AGATCT A^GATCT BpuAI 1186 GAAGAC GAAGAC(2/6) BsaAI 1299 CACGTA [CT]AC^GT[GA] BsaI 909 GGTCTC GGTCTC(1/5) BsaWI 1403 ACCGGA [AT]^CCGG[AT] BsiWI 1301 CGTACG C^GTACG Bsp1407I 1337 TGTACA T^GTACA BspDI 499 ATCGAT AT^CGAT BsrGI 1337 TGTACA T^GTACA BsrI 1572 CCAGT ACTGG(1/-1) BssHII 1444 GCGCGC G^CGCGC Bst1107I 64 GTATAC GTA^TAC BstAPI 162 GCAGCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 380 AGATCT [GA]^GATC[CT] BstZ17I 64 GTATAC GTA^TAC ClaI 499 ATCGAT AT^CGAT DrdI 21 GACGCGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FspI 869 TGCGCA TGC^GCA HincII 1735 GTCGAC GT[CT]^[GA]AC HindII 1735 GTCGAC GT[CT]^[GA]AC HindIII 242 AAGCTT A^AGCTT KspI 123 CCGCGG CCGC^GG MflI 380 AGATCT [GA]^GATC[CT] MluNI 1199 TGGCCA TGG^CCA MscI 1199 TGGCCA TGG^CCA NcoI 1520 CCATGG C^CATGG PflMI 687 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 1662 CAGCTG CAG^CTG SacI 928 GAGCTC GAGCT^C SacII 123 CCGCGG CCGC^GG SalI 1735 GTCGAC G^TCGAC SapI 129 GCTCTTC GCTCTTC(1/4) SgrAI 1047 CGCCGGCG C[GA]^CCGG[CT]G SspBI 1337 TGTACA T^GTACA StuI 1550 AGGCCT AGG^CCT TfiI 52 GAATC G^A[AT]TC Tsp509I 1365 AATT ^AATT Van91I 687 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 380 AGATCT [GA]^GATC[CT] XmnI 516 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AhdI 1245 GACGTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1264 GACTCCTGGTC Aor51HI 1227 AGCGCT AGC^GCT 1526 AGCGCT AspEI 1245 GACGTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1264 GACTCCTGGTC AspI 139 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC 349 GACTACGTC AvaI 664 CCCGAG C^[CT]CG[GA]G 1344 CTCGGG BbeI 271 GGCGCC GGCGC^C 1587 GGCGCC BcnI 998 CCCGG CC^[GC]GG 1839 CCGGG BglI 993 GCCCGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1307 GCCACGCGGGC BseRI 802 GAGGAG GAGGAG(10/8) 1420 GAGGAG BsmFI 1067 GGGAC GGGAC(10/14) 1253 GTCCC BsoBI 664 CCCGAG C^[CT]CG[GA]G 1344 CTCGGG DdeI 589 CTGAG C^T[ACGT]AG 931 CTCAG EagI 752 CGGCCG C^GGCCG 1209 CGGCCG Eam1105I 1245 GACGTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1264 GACTCCTGGTC EarI 130 CTCTTC CTCTTC(1/4) 1233 CTCTTC EclXI 752 CGGCCG C^GGCCG 1209 CGGCCG Eco47III 1227 AGCGCT AGC^GCT 1526 AGCGCT Eco52I 752 CGGCCG C^GGCCG 1209 CGGCCG KasI 271 GGCGCC G^GCGCC 1587 GGCGCC Ksp632I 130 CTCTTC CTCTTC(1/4) 1233 CTCTTC MaeIII 935 GTGAC ^GT[ACGT]AC 1475 GTGAC NarI 271 GGCGCC GG^CGCC 1587 GGCGCC NciI 998 CCCGG CC^[GC]GG 1839 CCGGG PflFI 139 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC 349 GACTACGTC SbfI 450 CCTGCAGG CCTGCA^GG 630 CCTGCAGG SfoI 271 GGCGCC GGC^GCC 1587 GGCGCC SphI 233 GCATGC GCATG^C 1599 GCATGC Sse8387I 450 CCTGCAGG CCTGCA^GG 630 CCTGCAGG Tsp45I 935 GTGAC ^GT[GC]AC 1475 GTGAC Tth111I 139 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC 349 GACTACGTC ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AluI AlwNI AspHI AvaII BanI BanII BbvI BmyI BpmI BsaHI BsaJI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DpnII DsaI EaeI EcoNI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NaeI NdeII NgoMIV NlaIII NlaIV NspI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TseI TspRI TthHB8I