(Circular) (Six-base) MAPSORT of: pFN21AE3954.seq Check: 1026 from: 1 to: 6553 pFN21AE3954 6553 bp With 182 enzymes: SgfI * March 28, 2012 11:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2385 2957 3691 2385 Size: 572 734 5247 Fragments arranged by size: 5247 734 572 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2148 4363 278 Size: 53 83 186 936 612 2215 2468 Fragments arranged by size: 2468 2215 936 612 186 83 53 AccI GT'mk_AC Cuts at: 1937 2070 2400 2589 3741 3896 1937 Size: 133 330 189 1152 155 4594 Fragments arranged by size: 4594 1152 330 189 155 133 Acc65I G'GTAC_C Cuts at: 3874 3874 Size: 6553 AclI AA'CG_TT Cuts at: 1563 4680 5053 1563 Size: 3117 373 3063 Fragments arranged by size: 3117 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3225 4616 6000 6268 1632 Size: 1593 1391 1384 268 1917 Fragments arranged by size: 1917 1593 1391 1384 268 AfeI AGC'GCT Cuts at: 3234 3533 3234 Size: 299 6254 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6356 AflIII A'CryG_T Cuts at: 1203 3417 5452 6429 1203 Size: 2214 2035 977 1327 Fragments arranged by size: 2214 2035 1327 977 AgeI A'CCGG_T Cuts at: 5384 5384 Size: 6553 AhdI GACnn_n'nnGTC Cuts at: 3255 3274 5282 3255 Size: 19 2008 4526 Fragments arranged by size: 4526 2008 19 AleI CACnn'nnGTG Cuts at: 1166 3018 1166 Size: 1852 4701 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2117 2624 2888 3407 1184 Size: 933 507 264 519 4330 Fragments arranged by size: 4330 933 519 507 264 AlwNI CAG_nnn'CTG Cuts at: 2110 2464 2581 2656 2698 4296 5868 2110 Size: 354 117 75 42 1598 1572 2795 Fragments arranged by size: 2795 1598 1572 354 117 75 42 ApaLI G'TGCA_C Cuts at: 4610 5766 4610 Size: 1156 5397 ApoI r'AATT_y Cuts at: 1493 3862 4088 4240 6349 1493 Size: 2369 226 152 2109 1697 Fragments arranged by size: 2369 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5107 160 Size: 872 4075 1606 Fragments arranged by size: 4075 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6553 AvaI C'yCGr_G Cuts at: 1870 1943 2669 3349 3878 1870 Size: 73 726 680 529 4545 Fragments arranged by size: 4545 726 680 529 73 BamHI G'GATC_C Cuts at: 3883 4250 3883 Size: 367 6186 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2276 3320 3592 3834 3874 Size: 325 217 627 489 1044 272 242 40 Cuts at: 3874 5329 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1044 627 489 325 272 242 217 40 BanII G_rGCy'C Cuts at: 1391 2136 2295 2937 3872 1391 Size: 745 159 642 935 4072 Fragments arranged by size: 4072 935 745 642 159 BbeI G_GCGC'C Cuts at: 2280 3596 2280 Size: 1316 5237 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3198 928 Size: 971 1299 4283 Fragments arranged by size: 4283 1299 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3721 3755 4744 4778 1903 Size: 34 1784 34 989 34 3678 Fragments arranged by size: 3678 1784 989 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4445 5655 4445 Size: 1210 5343 BclI T'GATC_A Cuts at: 1548 1548 Size: 6553 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3004 3318 5164 Size: 107 122 71 631 605 1332 314 1846 Cuts at: 5164 136 Size: 1525 Fragments arranged by size: 1846 1525 1332 631 605 314 122 107 71 BglII A'GATC_T Cuts at: 2385 6548 2385 Size: 4163 2390 BlpI GC'TnA_GC Cuts at: 3970 3970 Size: 6553 Bme1580I G_kGCm'C Cuts at: 1790 3323 4614 5770 1790 Size: 1533 1291 1156 2573 Fragments arranged by size: 2573 1533 1291 1156 BmgBI CAC'GTC Cuts at: 1318 3418 3541 1318 Size: 2100 123 4330 Fragments arranged by size: 4330 2100 123 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4284 5242 442 Size: 416 53 83 399 24 2867 958 1753 Fragments arranged by size: 2867 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6553 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2485 2485 Size: 6553 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2384 2441 2720 2951 3809 5213 1239 Size: 1145 57 279 231 858 1404 2579 Fragments arranged by size: 2579 1404 1145 858 279 231 57 Bpu10I CC'TnA_GC Cuts at: 2594 2594 Size: 6553 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2074 2279 2468 4678 5543 5841 2074 Size: 205 189 2210 865 298 2786 Fragments arranged by size: 2786 2210 865 298 205 189 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2920 5216 882 Size: 617 1421 2296 2219 Fragments arranged by size: 2296 2219 1421 617 BsaAI yAC'GTr Cuts at: 493 1204 3306 4344 493 Size: 711 2102 1038 2702 Fragments arranged by size: 2702 2102 1038 711 BsaBI GATnn'nnATC Cuts at: 6547 6547 Size: 6553 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2145 2277 2961 2990 Size: 53 83 186 936 612 132 684 29 Cuts at: 2990 3593 3738 4360 4742 5377 275 Size: 603 145 622 382 635 1451 Fragments arranged by size: 1451 936 684 635 622 612 603 382 186 145 132 83 53 29 BsaWI w'CCGG_w Cuts at: 3408 4986 5384 5658 5805 6216 3408 Size: 1578 398 274 147 411 3745 Fragments arranged by size: 3745 1578 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2204 2234 3167 3197 1178 Size: 30 165 30 801 30 933 30 4534 Fragments arranged by size: 4534 933 801 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2822 3440 1186 Size: 1636 618 4299 Fragments arranged by size: 4299 1636 618 BseYI C'CCAG_C Cuts at: 2076 2841 3031 3544 3669 5756 6252 2076 Size: 765 190 513 125 2087 496 2377 Fragments arranged by size: 2377 2087 765 513 496 190 125 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2224 2630 2726 3490 3719 1163 Size: 573 488 406 96 764 229 3997 Fragments arranged by size: 3997 764 573 488 406 229 96 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2760 3217 3712 4003 4764 5792 Size: 275 186 759 457 495 291 761 1028 Cuts at: 5792 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 759 495 457 291 275 186 BsiHKAI G_wGCw'C Cuts at: 2628 2768 2937 4614 4699 5770 2628 Size: 140 169 1677 85 1071 3411 Fragments arranged by size: 3411 1677 1071 169 140 85 BsiWI C'GTAC_G Cuts at: 3306 3306 Size: 6553 BsmI GAATG_Cn' Cuts at: 4064 4157 4064 Size: 93 6460 BsmBI CGTCTCn'nnnn_ Cuts at: 2129 2360 2572 2981 3242 2129 Size: 231 212 409 261 5440 Fragments arranged by size: 5440 409 261 231 212 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2136 2295 2628 2768 2937 3323 3872 Size: 399 346 159 333 140 169 386 549 Cuts at: 3872 4614 4699 5770 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 549 399 386 346 333 169 159 140 85 BspEI T'CCGG_A Cuts at: 6216 6216 Size: 6553 BspHI T'CATG_A Cuts at: 4442 6172 4442 Size: 1730 4823 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2199 2385 2463 2745 2957 3399 3691 3753 Size: 1355 186 78 282 212 442 292 62 Cuts at: 3753 3908 844 Size: 155 3489 Fragments arranged by size: 3489 1355 442 292 282 212 186 155 78 62 BsrBI CCG'CTC Cuts at: 1394 1823 2719 2923 3430 4440 1394 Size: 429 896 204 507 1010 3507 Fragments arranged by size: 3507 1010 896 507 429 204 BsrDI GCAATG_nn' Cuts at: 65 1248 5048 5222 65 Size: 1183 3800 174 1396 Fragments arranged by size: 3800 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2755 3053 3287 5197 5384 1700 Size: 110 945 298 234 1910 187 2869 Fragments arranged by size: 2869 1910 945 298 234 187 110 BsrGI T'GTAC_A Cuts at: 96 3342 96 Size: 3246 3307 BssHII G'CGCG_C Cuts at: 1925 3449 1925 Size: 1524 5029 BssSI C'ACGA_G Cuts at: 4613 5625 4613 Size: 1012 5541 BstAPI GCAn_nnn'nTGC Cuts at: 794 2173 794 Size: 1379 5174 BstBI TT'CG_AA Cuts at: 5357 5357 Size: 6553 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6553 BstYI r'GATC_y Cuts at: 1972 2385 3883 4250 4635 4652 6104 6190 6202 Size: 413 1498 367 385 17 1452 86 12 Cuts at: 6202 6548 1972 Size: 346 1977 Fragments arranged by size: 1977 1498 1452 413 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2071 2071 Size: 6553 BtgI C'CryG_G Cuts at: 513 1065 2128 2301 3444 3525 3794 513 Size: 552 1063 173 1143 81 269 3272 Fragments arranged by size: 3272 1143 1063 552 269 173 81 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2168 3034 3522 3742 487 Size: 683 56 942 866 488 220 3298 Fragments arranged by size: 3298 942 866 683 488 220 56 BtsI GCAGTG_nn' Cuts at: 2657 2981 3575 3636 3678 4069 4863 4883 2657 Size: 324 594 61 42 391 794 20 4327 Fragments arranged by size: 4327 794 594 391 324 61 42 20 ClaI AT'CG_AT Cuts at: 1554 2505 1554 Size: 951 5602 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6518 DraI TTT'AAA Cuts at: 3857 3857 Size: 6553 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2032 5560 809 Size: 507 716 3528 1802 Fragments arranged by size: 3528 1802 716 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2039 2157 2196 Size: 54 1425 50 275 18 209 118 39 Cuts at: 2196 2757 3204 3214 3285 3310 3797 4000 4889 Size: 561 447 10 71 25 487 203 889 Cuts at: 4889 6518 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 561 487 447 275 209 203 118 71 54 50 43 39 25 18 10 EagI C'GGCC_G Cuts at: 1537 1812 2757 3214 4000 1537 Size: 275 945 457 786 4090 Fragments arranged by size: 4090 945 786 457 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2141 3244 4483 1353 Size: 788 1103 1239 3423 Fragments arranged by size: 3423 1239 1103 788 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3022 3760 5140 5514 5660 387 Size: 1064 138 1433 738 1380 374 146 1280 Fragments arranged by size: 1433 1380 1280 1064 738 374 146 138 EcoICRI GAG'CTC Cuts at: 2935 2935 Size: 6553 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2384 2441 2720 2951 3225 3809 4616 Size: 393 752 57 279 231 274 584 807 Cuts at: 4616 5213 6000 6268 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 807 787 752 597 584 393 279 274 268 231 57 EcoNI CCTnn'n_nnAGG Cuts at: 2423 2636 3749 2423 Size: 213 1113 5227 Fragments arranged by size: 5227 1113 213 EcoRI G'AATT_C Cuts at: 3862 4240 3862 Size: 378 6175 FspI TGC'GCA Cuts at: 2876 5059 2876 Size: 2183 4370 HaeII r_GCGC'y Cuts at: 1678 2280 2873 2893 3163 3236 3535 3596 5700 Size: 602 593 20 270 73 299 61 2104 Cuts at: 5700 1678 Size: 2531 Fragments arranged by size: 2531 2104 602 593 299 270 73 61 20 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3077 3109 3241 3260 3273 Size: 32 135 32 1505 32 132 19 13 Cuts at: 3273 3292 5194 5226 5268 5300 1373 Size: 19 1902 32 42 32 2626 Fragments arranged by size: 2626 1902 1505 135 132 42 32 32 32 32 32 19 19 13 HincII GTy'rAC Cuts at: 669 1657 1938 3742 3897 4143 669 Size: 988 281 1804 155 246 3079 Fragments arranged by size: 3079 1804 988 281 246 155 HindIII A'AGCT_T Cuts at: 748 2247 748 Size: 1499 5054 HpaI GTT'AAC Cuts at: 4143 4143 Size: 6553 KasI G'GCGC_C Cuts at: 2276 3592 2276 Size: 1316 5237 KpnI G_GTAC'C Cuts at: 3878 3878 Size: 6553 MfeI C'AATT_G Cuts at: 4152 4152 Size: 6553 MluI A'CGCG_T Cuts at: 6429 6429 Size: 6553 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5667 5851 1305 Size: 4362 184 2007 Fragments arranged by size: 4362 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3206 10 Size: 54 1425 343 1374 3357 Fragments arranged by size: 3357 1425 1374 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2237 3018 3200 4511 4870 5029 518 Size: 648 1071 781 182 1311 359 159 2042 Fragments arranged by size: 2042 1311 1071 781 648 359 182 159 MspA1I CmG'CkG Cuts at: 1606 2076 2107 2130 2614 2995 3123 3598 3669 Size: 470 31 23 484 381 128 475 71 Cuts at: 3669 3969 4646 5794 6039 1606 Size: 300 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 484 475 470 381 300 245 128 71 31 23 NaeI GCC'GGC Cuts at: 2757 3055 3289 2757 Size: 298 234 6021 Fragments arranged by size: 6021 298 234 NarI GG'CG_CC Cuts at: 2277 3593 2277 Size: 1316 5237 NcoI C'CATG_G Cuts at: 513 1065 3525 513 Size: 552 2460 3541 Fragments arranged by size: 3541 2460 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6553 NgoMIV G'CCGG_C Cuts at: 2755 3053 3287 2755 Size: 298 234 6021 Fragments arranged by size: 6021 298 234 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6553 NotI GC'GGCC_GC Cuts at: 4000 4000 Size: 6553 NspI r_CATG'y Cuts at: 1132 1229 2242 3199 3469 3608 3911 5456 1132 Size: 97 1013 957 270 139 303 1545 2229 Fragments arranged by size: 2229 1545 1013 957 303 270 139 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6553 PciI A'CATG_T Cuts at: 5452 5452 Size: 6553 PflMI CCAn_nnn'nTGG Cuts at: 1108 2698 1108 Size: 1590 4963 PfoI T'CCnGG_A Cuts at: 2361 2361 Size: 6553 PmeI GTTT'AAAC Cuts at: 3857 3857 Size: 6553 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4622 4654 6135 6167 4622 Size: 32 1481 32 5008 Fragments arranged by size: 5008 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6553 PsiI TTA'TAA Cuts at: 4123 4123 Size: 6553 PstI C_TGCA'G Cuts at: 830 2106 2385 2460 2562 2640 2652 3905 830 Size: 1276 279 75 102 78 12 1253 3478 Fragments arranged by size: 3478 1276 1253 279 102 78 75 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6553 PvuII CAG'CTG Cuts at: 3669 3669 Size: 6553 SacI G_AGCT'C Cuts at: 2937 2937 Size: 6553 SacII CC_GC'GG Cuts at: 2131 2131 Size: 6553 SalI G'TCGA_C Cuts at: 1936 3740 3895 1936 Size: 1804 155 4594 Fragments arranged by size: 4594 1804 155 SapI GCTCTTCn'nnn_ Cuts at: 2141 2141 Size: 6553 SbfI CC_TGCA'GG Cuts at: 2460 2640 3905 2460 Size: 180 1265 5108 Fragments arranged by size: 5108 1265 180 ScaI AGT'ACT Cuts at: 4801 6513 4801 Size: 1712 4841 SfcI C'TryA_G Cuts at: 826 1045 2102 2381 2456 2476 2558 2636 2648 Size: 219 1057 279 75 20 82 78 12 Cuts at: 2648 3559 3664 3901 5036 5717 5908 826 Size: 911 105 237 1135 681 191 1471 Fragments arranged by size: 1471 1135 1057 911 681 279 237 219 191 105 82 78 75 20 12 SfoI GGC'GCC Cuts at: 2278 3594 2278 Size: 1316 5237 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6553 SgrAI Cr'CCGG_yG Cuts at: 1700 3053 1700 Size: 1353 5200 SmaI CCC'GGG Cuts at: 1872 3880 1872 Size: 2008 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2089 2294 2483 4657 5558 5820 Size: 197 926 146 205 189 2174 901 262 Cuts at: 5820 820 Size: 1553 Fragments arranged by size: 2174 1553 926 901 262 205 197 189 146 SnaBI TAC'GTA Cuts at: 493 4344 493 Size: 3851 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6553 SphI G_CATG'C Cuts at: 1132 2242 3608 3911 1132 Size: 1110 1366 303 3774 Fragments arranged by size: 3774 1366 1110 303 SspI AAT'ATT Cuts at: 5 52 4477 5 Size: 47 4425 2081 Fragments arranged by size: 4425 2081 47 StuI AGG'CCT Cuts at: 1517 3557 1517 Size: 2040 4513 StyI C'CwwG_G Cuts at: 513 1065 2778 3296 3525 3750 3992 513 Size: 552 1713 518 229 225 242 3074 Fragments arranged by size: 3074 1713 552 518 242 229 225 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3467 4580 4597 4750 4935 5380 325 Size: 1255 1887 1113 17 153 185 445 1498 Fragments arranged by size: 1887 1498 1255 1113 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3342 4799 6511 Size: 274 80 33 51 1443 1365 1457 1712 Cuts at: 6511 96 Size: 138 Fragments arranged by size: 1712 1457 1443 1365 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6513 6545 6513 Size: 32 6521 Tth111I GACn'n_nGTC Cuts at: 1316 2147 2357 1316 Size: 831 210 5512 Fragments arranged by size: 5512 831 210 XbaI T'CTAG_A Cuts at: 3889 3889 Size: 6553 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6553 XmaI C'CCGG_G Cuts at: 1870 3878 1870 Size: 2008 4545 XmnI GAAnn'nnTTC Cuts at: 2525 4682 2525 Size: 2157 4396 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2146 4361 276 Size: 53 83 186 936 612 2215 2468 Fragments arranged by size: 2468 2215 936 612 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI BstXI Bsu36I DraIII EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI XcmI