(Linear) (Six-base) MAPSORT of: KIEE3954.seq Check: 5465 from: 1 to: 1848 KIEE3954.orf With 182 enzymes: SgfI * March 22, 2012 16:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 380 952 1686 1848 Size: 380 572 734 162 Fragments arranged by size: 734 572 380 162 AatII G_ACGT'C Cuts at: 0 143 1848 Size: 143 1705 AccI GT'mk_AC Cuts at: 0 65 395 584 1736 1848 Size: 65 330 189 1152 112 Fragments arranged by size: 1152 330 189 112 65 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1220 1848 Size: 1220 628 AfeI AGC'GCT Cuts at: 0 1229 1528 1848 Size: 1229 299 320 Fragments arranged by size: 1229 320 299 AflIII A'CryG_T Cuts at: 0 1412 1848 Size: 1412 436 AhdI GACnn_n'nnGTC Cuts at: 0 1250 1269 1848 Size: 1250 19 579 Fragments arranged by size: 1250 579 19 AleI CACnn'nnGTG Cuts at: 0 1013 1848 Size: 1013 835 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 112 619 883 1402 1848 Size: 112 507 264 519 446 Fragments arranged by size: 519 507 446 264 112 AlwNI CAG_nnn'CTG Cuts at: 0 105 459 576 651 693 1848 Size: 105 354 117 75 42 1155 Fragments arranged by size: 1155 354 117 105 75 42 AvaI C'yCGr_G Cuts at: 0 664 1344 1848 Size: 664 680 504 Fragments arranged by size: 680 664 504 BanI G'GyrC_C Cuts at: 0 271 1315 1587 1829 1848 Size: 271 1044 272 242 19 Fragments arranged by size: 1044 272 271 242 19 BanII G_rGCy'C Cuts at: 0 131 290 932 1848 Size: 131 159 642 916 Fragments arranged by size: 916 642 159 131 BbeI G_GCGC'C Cuts at: 0 275 1591 1848 Size: 275 1316 257 Fragments arranged by size: 1316 275 257 BbsI GAAGACnn'nnnn_ Cuts at: 0 1193 1848 Size: 1193 655 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1716 1750 1848 Size: 1716 34 98 Fragments arranged by size: 1716 98 34 BglI GCCn_nnn'nGGC Cuts at: 0 999 1313 1848 Size: 999 314 535 Fragments arranged by size: 999 535 314 BglII A'GATC_T Cuts at: 0 380 1848 Size: 380 1468 Bme1580I G_kGCm'C Cuts at: 0 1318 1848 Size: 1318 530 BmgBI CAC'GTC Cuts at: 0 1413 1536 1848 Size: 1413 123 312 Fragments arranged by size: 1413 312 123 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 480 1848 Size: 480 1368 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 379 436 715 946 1804 1848 Size: 379 57 279 231 858 44 Fragments arranged by size: 858 379 279 231 57 44 Bpu10I CC'TnA_GC Cuts at: 0 589 1848 Size: 589 1259 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 69 274 463 1848 Size: 69 205 189 1385 Fragments arranged by size: 1385 205 189 69 BsaI GGTCTCn'nnnn_ Cuts at: 0 915 1848 Size: 915 933 BsaAI yAC'GTr Cuts at: 0 1301 1848 Size: 1301 547 BsaHI Gr'CG_yC Cuts at: 0 140 272 956 985 1588 1733 1848 Size: 140 132 684 29 603 145 115 Fragments arranged by size: 684 603 145 140 132 115 29 BsaWI w'CCGG_w Cuts at: 0 1403 1848 Size: 1403 445 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 199 229 1162 1192 1848 Size: 199 30 933 30 656 Fragments arranged by size: 933 656 199 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 817 1435 1848 Size: 817 618 413 Fragments arranged by size: 817 618 413 BseYI C'CCAG_C Cuts at: 0 71 836 1026 1539 1664 1848 Size: 71 765 190 513 125 184 Fragments arranged by size: 765 513 190 184 125 71 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 219 625 721 1485 1714 1848 Size: 219 406 96 764 229 134 Fragments arranged by size: 764 406 229 219 134 96 BsiEI CG_ry'CG Cuts at: 0 755 1212 1707 1848 Size: 755 457 495 141 Fragments arranged by size: 755 495 457 141 BsiHKAI G_wGCw'C Cuts at: 0 623 763 932 1848 Size: 623 140 169 916 Fragments arranged by size: 916 623 169 140 BsiWI C'GTAC_G Cuts at: 0 1301 1848 Size: 1301 547 BsmBI CGTCTCn'nnnn_ Cuts at: 0 124 355 567 976 1237 1848 Size: 124 231 212 409 261 611 Fragments arranged by size: 611 409 261 231 212 124 Bsp1286I G_dGCh'C Cuts at: 0 131 290 623 763 932 1318 1848 Size: 131 159 333 140 169 386 530 Fragments arranged by size: 530 386 333 169 159 140 131 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 194 380 458 740 952 1394 1686 1748 Size: 194 186 78 282 212 442 292 62 Cuts at: 1748 1848 Size: 100 Fragments arranged by size: 442 292 282 212 194 186 100 78 62 BsrBI CCG'CTC Cuts at: 0 714 918 1425 1848 Size: 714 204 507 423 Fragments arranged by size: 714 507 423 204 BsrFI r'CCGG_y Cuts at: 0 750 1048 1282 1848 Size: 750 298 234 566 Fragments arranged by size: 750 566 298 234 BsrGI T'GTAC_A Cuts at: 0 1337 1848 Size: 1337 511 BssHII G'CGCG_C Cuts at: 0 1444 1848 Size: 1444 404 BstAPI GCAn_nnn'nTGC Cuts at: 0 168 1848 Size: 168 1680 BstYI r'GATC_y Cuts at: 0 380 1848 Size: 380 1468 BstZ17I GTA'TAC Cuts at: 0 66 1848 Size: 66 1782 BtgI C'CryG_G Cuts at: 0 123 296 1439 1520 1789 1848 Size: 123 173 1143 81 269 59 Fragments arranged by size: 1143 269 173 123 81 59 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 163 1029 1517 1737 1848 Size: 163 866 488 220 111 Fragments arranged by size: 866 488 220 163 111 BtsI GCAGTG_nn' Cuts at: 0 652 976 1570 1631 1673 1848 Size: 652 324 594 61 42 175 Fragments arranged by size: 652 594 324 175 61 42 ClaI AT'CG_AT Cuts at: 0 500 1848 Size: 500 1348 DrdI GACnn_nn'nnGTC Cuts at: 0 27 1848 Size: 27 1821 EaeI y'GGCC_r Cuts at: 0 34 152 191 752 1199 1209 1280 1305 Size: 34 118 39 561 447 10 71 25 Cuts at: 1305 1792 1848 Size: 487 56 Fragments arranged by size: 561 487 447 118 71 56 39 34 25 10 EagI C'GGCC_G Cuts at: 0 752 1209 1848 Size: 752 457 639 Fragments arranged by size: 752 639 457 EarI CTCTTCn'nnn_ Cuts at: 0 136 1239 1848 Size: 136 1103 609 Fragments arranged by size: 1103 609 136 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1017 1755 1848 Size: 1017 738 93 Fragments arranged by size: 1017 738 93 EcoICRI GAG'CTC Cuts at: 0 930 1848 Size: 930 918 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 379 436 715 946 1220 1804 1848 Size: 379 57 279 231 274 584 44 Fragments arranged by size: 584 379 279 274 231 57 44 EcoNI CCTnn'n_nnAGG Cuts at: 0 418 631 1744 1848 Size: 418 213 1113 104 Fragments arranged by size: 1113 418 213 104 FspI TGC'GCA Cuts at: 0 871 1848 Size: 871 977 HaeII r_GCGC'y Cuts at: 0 275 868 888 1158 1231 1530 1591 1848 Size: 275 593 20 270 73 299 61 257 Fragments arranged by size: 593 299 275 270 257 73 61 20 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1072 1104 1236 1255 1268 1287 1848 Size: 1072 32 132 19 13 19 561 Fragments arranged by size: 1072 561 132 32 19 19 13 HincII GTy'rAC Cuts at: 0 1737 1848 Size: 1737 111 HindIII A'AGCT_T Cuts at: 0 242 1848 Size: 242 1606 KasI G'GCGC_C Cuts at: 0 271 1587 1848 Size: 271 1316 261 Fragments arranged by size: 1316 271 261 MscI TGG'CCA Cuts at: 0 1201 1848 Size: 1201 647 MslI CAynn'nnrTG Cuts at: 0 232 1013 1195 1848 Size: 232 781 182 653 Fragments arranged by size: 781 653 232 182 MspA1I CmG'CkG Cuts at: 0 71 102 125 609 990 1118 1593 1664 Size: 71 31 23 484 381 128 475 71 Cuts at: 1664 1848 Size: 184 Fragments arranged by size: 484 475 381 184 128 71 71 31 23 NaeI GCC'GGC Cuts at: 0 752 1050 1284 1848 Size: 752 298 234 564 Fragments arranged by size: 752 564 298 234 NarI GG'CG_CC Cuts at: 0 272 1588 1848 Size: 272 1316 260 Fragments arranged by size: 1316 272 260 NcoI C'CATG_G Cuts at: 0 1520 1848 Size: 1520 328 NgoMIV G'CCGG_C Cuts at: 0 750 1048 1282 1848 Size: 750 298 234 566 Fragments arranged by size: 750 566 298 234 NspI r_CATG'y Cuts at: 0 237 1194 1464 1603 1848 Size: 237 957 270 139 245 Fragments arranged by size: 957 270 245 237 139 PflMI CCAn_nnn'nTGG Cuts at: 0 693 1848 Size: 693 1155 PfoI T'CCnGG_A Cuts at: 0 356 1848 Size: 356 1492 PstI C_TGCA'G Cuts at: 0 101 380 455 557 635 647 1848 Size: 101 279 75 102 78 12 1201 Fragments arranged by size: 1201 279 102 101 78 75 12 PvuII CAG'CTG Cuts at: 0 1664 1848 Size: 1664 184 SacI G_AGCT'C Cuts at: 0 932 1848 Size: 932 916 SacII CC_GC'GG Cuts at: 0 126 1848 Size: 126 1722 SalI G'TCGA_C Cuts at: 0 1735 1848 Size: 1735 113 SapI GCTCTTCn'nnn_ Cuts at: 0 136 1848 Size: 136 1712 SbfI CC_TGCA'GG Cuts at: 0 455 635 1848 Size: 455 180 1213 Fragments arranged by size: 1213 455 180 SfcI C'TryA_G Cuts at: 0 97 376 451 471 553 631 643 1554 Size: 97 279 75 20 82 78 12 911 Cuts at: 1554 1659 1848 Size: 105 189 Fragments arranged by size: 911 279 189 105 97 82 78 75 20 12 SfoI GGC'GCC Cuts at: 0 273 1589 1848 Size: 273 1316 259 Fragments arranged by size: 1316 273 259 SgrAI Cr'CCGG_yG Cuts at: 0 1048 1848 Size: 1048 800 SmlI C'TyrA_G Cuts at: 0 84 289 478 1848 Size: 84 205 189 1370 Fragments arranged by size: 1370 205 189 84 SphI G_CATG'C Cuts at: 0 237 1603 1848 Size: 237 1366 245 Fragments arranged by size: 1366 245 237 StuI AGG'CCT Cuts at: 0 1552 1848 Size: 1552 296 StyI C'CwwG_G Cuts at: 0 773 1291 1520 1745 1848 Size: 773 518 229 225 103 Fragments arranged by size: 773 518 229 225 103 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1462 1848 Size: 1462 386 TatI w'GTAC_w Cuts at: 0 1337 1848 Size: 1337 511 Tth111I GACn'n_nGTC Cuts at: 0 142 352 1848 Size: 142 210 1496 Fragments arranged by size: 1496 210 142 XmnI GAAnn'nnTTC Cuts at: 0 520 1848 Size: 520 1328 ZraI GAC'GTC Cuts at: 0 141 1848 Size: 141 1707 Enzymes that do cut: AarI AatII AccI AcuI AfeI AflIII AhdI AleI AlfI AlwNI AvaI BanI BanII BbeI BbsI BcgI BglI BglII Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstYI BstZ17I BtgI BtgZI BtsI ClaI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI FspI HaeII Hin4I HincII HindIII KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PflMI PfoI PstI PvuII SacI SacII SalI SapI SbfI SfcI SfoI SgrAI SmlI SphI StuI StyI TaqII TatI Tth111I XmnI ZraI Enzymes that do not cut: Acc65I AclI AflII AgeI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BlpI BmrI BmtI BsaBI BsmI BspEI BspHI BsrDI BssSI BstBI BstEII BstXI Bsu36I CspCI DraI DraIII EcoO109I EcoRI EcoRV FalI FseI FspAI HpaI KpnI MfeI MluI MmeI NdeI NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SanDI ScaI SexAI SfiI SgfI SmaI SnaBI SpeI SrfI SspI SwaI TstI XbaI XcmI XhoI XmaI