################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BanI BbeI BbrPI BcgI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoRI EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HapII HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tth111I TthHB8I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AciI 360 GCGG CCGC(-3/-1) AcsI 556 GAATTT [GA]^AATT[CT] ApoI 556 GAATTT [GA]^AATT[CT] BbsI 630 GAAGAC GAAGAC(2/6) BbvCI 655 GCTGAGG CCTCAGC(-5/-2) BciVI 545 GGATAC GTATCC(6/5) BglII 282 AGATCT A^GATCT BpuAI 630 GAAGAC GAAGAC(2/6) BsaI 362 GGTCTC GGTCTC(1/5) BsaJI 375 CCGTGG C^C[ACGT][ACGT]GG BsmFI 44 GTCCC GGGAC(10/14) BspHI 764 TCATGA T^CATGA BsrBI 358 GAGCGG CCGCTC(-3/-3) BstYI 282 AGATCT [GA]^GATC[CT] DsaI 375 CCGTGG C^C[GA][CT]GG EcoNI 393 CCTCACGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HgaI 633 GACGC GACGC(5/10) MaeII 23 ACGT A^CGT MflI 282 AGATCT [GA]^GATC[CT] MseI 20 TTAA T^TAA MslI 114 CATCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 162 CAGCTG C[AC]G^C[GT]G PflMI 262 CCAGACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 745 CTGCAG CTGCA^G PvuII 162 CAGCTG CAG^CTG RcaI 764 TCATGA T^CATGA SacI 694 GAGCTC GAGCT^C TfiI 761 GAATC G^A[AT]TC Tru9I 20 TTAA T^TAA Tsp509I 557 AATT ^AATT Van91I 262 CCAGACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 81 TCTAGA T^CTAGA XhoII 282 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AfaI 25 GTAC GT^AC 526 GTAC AspHI 370 GAGCAC G[AT]GC[AT]^C 694 GAGCTC BanII 258 GAGCCC G[GA]GC[CT]^C 694 GAGCTC BclI 291 TGATCA T^GATCA 561 TGATCA BfaI 82 CTAG C^TAG 667 CTAG BpmI 430 CTCCAG CTGGAG(16/14) 55 CTGGAG BsiHKAI 370 GAGCAC G[AT]GC[AT]^C 694 GAGCTC BstEII 36 GGTGACC G^GT[ACGT]ACC 379 GGTCACC BstF5I 576 CATCC GGATG(2/0) 660 GGATG BstPI 36 GGTGACC G^GT[ACGT]ACC 379 GGTCACC BstXI 47 CCATCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 224 CCAACATAGTGG Cac8I 109 GCCTGC GC[ACGT]^[ACGT]GC 347 GCTGGC EcoO65I 36 GGTGACC G^GT[ACGT]ACC 379 GGTCACC FbaI 291 TGATCA T^GATCA 561 TGATCA FokI 576 CATCC GGATG(9/13) 660 GGATG HincII 63 GTTGAC GT[CT]^[GA]AC 729 GTCAAC HindII 63 GTTGAC GT[CT]^[GA]AC 729 GTCAAC MaeI 82 CTAG C^TAG 667 CTAG RsaI 25 GTAC GT^AC 526 GTAC SfcI 202 CTACAG C^T[GA][CT]AG 745 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AluI AlwNI BalI BbvI BmyI BseRI BsiYI BslI BsmAI Bsp1286I BssKI BstNI DdeI DpnII EaeI EarI EcoRII Fnu4HI HaeIII HinfI HphI ItaI Ksp632I MaeIII MboI MboII MluNI MnlI MscI MvaI MwoI NdeII NlaIII PleI Sau3AI ScrFI SfaNI SmlI TseI Tsp45I TspRI