Restriction map for
pFN21AE3952
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# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII Acc65I AccI AclI AscI
AseI AsnI Asp718I AspI BamHI
BcgI BglII BsiEI BsiWI Bsp1407I
BspDI BsrGI BssHII Bst1107I BstAPI
BstZ17I ClaI CpoI DrdI EagI
EclXI Eco52I EcoRV FseI HpaI
KpnI MfeI MluI MunI NcoI
NdeI NotI NruI PacI PflFI
PmeI PshBI Psp1406I PvuI RsrII
SalI ScaI SexAI SfiI SnaBI
SpeI SphI SspBI SwaI Tth111I
XbaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccIII 3419 TCCGGA T^CCGGA
AflII 1801 CTTAAG C^TTAAG
AgeI 1137 ACCGGT A^CCGGT
AhdI 2556 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4604 AGCGCT AGC^GCT
ApaI 4852 GGGCCC GGGCC^C
Asp700I 513 GAATACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2556 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 4455 TGCGCA TGC^GCA
AvrII 5097 CCTAGG C^CTAGG
BbeI 2571 GGCGCC GGCGC^C
BbrPI 2292 CACGTG CAC^GTG
BciVI 4663 GTATCC GTATCC(6/5)
BfrI 1801 CTTAAG C^TTAAG
BlnI 5097 CCTAGG C^CTAGG
BsaBI 4342 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3419 TCCGGA T^CCGGA
BsmI 658 GCATTC GAATGC(1/-1)
BspEI 3419 TCCGGA T^CCGGA
BspHI 4479 TCATGA T^CATGA
BspLU11I 2969 ACATGT A^CATGT
BsrBI 16 CCGCTC CCGCTC(-3/-3)
BstBI 2831 TTCGAA TT^CGAA
DraI 4080 TTTAAA TTT^AAA
DraIII 1379 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2556 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4604 AGCGCT AGC^GCT
EcoNI 4377 CCTGCATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 5113 GAATTC G^AATTC
FspI 4455 TGCGCA TGC^GCA
KasI 2571 GGCGCC G^GCGCC
KspI 4848 CCGCGG CCGC^GG
MamI 4342 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3419 TCCGGA T^CCGGA
NaeI 2427 GCCGGC GCC^GGC
NarI 2571 GGCGCC GG^CGCC
NgoMIV 2427 GCCGGC G^CCGGC
NheI 1479 GCTAGC G^CTAGC
NspV 2831 TTCGAA TT^CGAA
PinAI 1137 ACCGGT A^CCGGT
PmaCI 2292 CACGTG CAC^GTG
PmlI 2292 CACGTG CAC^GTG
PshAI 1459 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 4479 TCATGA T^CATGA
SacII 4848 CCGCGG CCGC^GG
SbfI 1452 CCTGCAGG CCTGCA^GG
SfoI 2571 GGCGCC GGC^GCC
SfuI 2831 TTCGAA TT^CGAA
SgrAI 1136 CACCGGTG C[GA]^CCGG[CT]G
Sse8387I 1452 CCTGCAGG CCTGCA^GG
SspI 2434 AATATT AAT^ATT
StuI 5011 AGGCCT AGG^CCT
XmnI 513 GAATACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 3674 CGCG CG^CG
4849 CGCG
AcsI 4233 AAATTC [GA]^AATT[CT]
5113 GAATTC
AcyI 2514 GGCGTC G[GA]^CG[CT]C
2571 GGCGCC
AflIII 2293 ACGTGT A^C[GA][CT]GT
2969 ACATGT
ApoI 4233 AAATTC [GA]^AATT[CT]
5113 GAATTC
BsaAI 2149 TACGTG [CT]AC^GT[GA]
2292 CACGTG
BsaHI 2514 GGCGTC G[GA]^CG[CT]C
2571 GGCGCC
BsaI 4507 GAGACC GGTCTC(1/5)
1991 GGTCTC
BssSI 3598 CACGAG CACGAG(-5/-1)
4467 CACGAG
BstEII 573 GGTTACC G^GT[ACGT]ACC
3771 GGTAACC
BstPI 573 GGTTACC G^GT[ACGT]ACC
3771 GGTAACC
BstUI 3674 CGCG CG^CG
4849 CGCG
EcoO65I 573 GGTTACC G^GT[ACGT]ACC
3771 GGTAACC
EcoT22I 656 ATGCAT ATGCA^T
1511 ATGCAT
HgaI 2515 GCGTC GACGC(5/10)
3792 GCGTC
Hin1I 2514 GGCGTC G[GA]^CG[CT]C
2571 GGCGCC
MseI 1802 TTAA T^TAA
4081 TTAA
MvnI 3674 CGCG CG^CG
4849 CGCG
NsiI 656 ATGCAT ATGCA^T
1511 ATGCAT
NspI 62 GCATGT [GA]CATG^[CT]
2969 ACATGT
PaeR7I 3266 CTCGAG C^TCGAG
4905 CTCGAG
SmaI 212 CCCGGG CCC^GGG
4394 CCCGGG
Tru9I 1802 TTAA T^TAA
4081 TTAA
XhoI 3266 CTCGAG C^TCGAG
4905 CTCGAG
XmaCI 212 CCCGGG C^CCGGG
4394 CCCGGG
XmaI 212 CCCGGG C^CCGGG
4394 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AciI AfaI AluI Alw44I AlwI
AlwNI AocI ApaLI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BclI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII