Restriction map for
pFN21AE3952
(Circular) (Six-base) MAPSORT of: pFN21AE3952.seq Check: 4175 from: 1 to: 9838
pFN21AE3952 9838 bp
With 182 enzymes: SgfI *
March 28, 2012 11:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5204 6970 5204
Size: 1766 8072
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7648 278
Size: 53 83 186 936 6112 2468
Fragments arranged by size:
6112 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 7181 1937
Size: 5244 4594
Acc65I G'GTAC_C
Cuts at: 7159 7159
Size: 9838
AclI AA'CG_TT
Cuts at: 1563 7965 8338 1563
Size: 6402 373 3063
Fragments arranged by size:
6402 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2575 2862 2958 3232 4946 5265 5487 6005
Size: 943 287 96 274 1714 319 222 518
Cuts at: 6005 6623 6920 7901 9285 9553 1632
Size: 618 297 981 1384 268 1917
Fragments arranged by size:
1917 1714 1384 981 943 618 518 319
297 287 274 268 222 96
AfeI AGC'GCT
Cuts at: 6611 6611
Size: 9838
AflII C'TTAA_G
Cuts at: 820 1017 3806 820
Size: 197 2789 6852
Fragments arranged by size:
6852 2789 197
AflIII A'CryG_T
Cuts at: 1203 4298 4974 8737 9714 1203
Size: 3095 676 3763 977 1327
Fragments arranged by size:
3763 3095 1327 977 676
AgeI A'CCGG_T
Cuts at: 3142 8669 3142
Size: 5527 4311
AhdI GACnn_n'nnGTC
Cuts at: 4566 8567 4566
Size: 4001 5837
AleI CACnn'nnGTG
Cuts at: 1166 3850 4109 4154 1166
Size: 2684 259 45 6850
Fragments arranged by size:
6850 2684 259 45
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3878 1184
Size: 2694 7144
AlwNI CAG_nnn'CTG
Cuts at: 2327 2867 2909 3349 3703 4178 4489 5011 5924
Size: 540 42 440 354 475 311 522 913
Cuts at: 5924 7000 7581 9153 2327
Size: 1076 581 1572 3012
Fragments arranged by size:
3012 1572 1076 913 581 540 522 475
440 354 311 42
ApaI G_GGCC'C
Cuts at: 6861 6861
Size: 9838
ApaLI G'TGCA_C
Cuts at: 2036 3953 4646 7895 9051 2036
Size: 1917 693 3249 1156 2823
Fragments arranged by size:
3249 2823 1917 1156 693
ApoI r'AATT_y
Cuts at: 1493 6238 7118 7147 7373 7525 9634 1493
Size: 4745 880 29 226 152 2109 1697
Fragments arranged by size:
4745 2109 1697 880 226 152 29
AseI AT'TA_AT
Cuts at: 160 1032 8392 160
Size: 872 7360 1606
Fragments arranged by size:
7360 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9838
AvaI C'yCGr_G
Cuts at: 1870 1943 2217 3337 3494 4076 4099 5271 5898
Size: 73 274 1120 157 582 23 1172 627
Cuts at: 5898 6399 6565 6910 7163 1870
Size: 501 166 345 253 4545
Fragments arranged by size:
4545 1172 1120 627 582 501 345 274
253 166 157 73 23
AvrII C'CTAG_G
Cuts at: 7102 7102
Size: 9838
BamHI G'GATC_C
Cuts at: 7168 7535 7168
Size: 367 9471
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2341 2851 3145 3283 3761
Size: 325 217 627 554 510 294 138 478
Cuts at: 3761 4148 4292 4392 4576 5883 7159 8614 618
Size: 387 144 100 184 1307 1276 1455 1842
Fragments arranged by size:
1842 1455 1307 1276 627 554 510 478
387 325 294 217 184 144 138 100
BanII G_rGCy'C
Cuts at: 1391 2190 2370 3501 3728 4049 4254 4685 5102
Size: 799 180 1131 227 321 205 431 417
Cuts at: 5102 5899 6752 6861 7157 1391
Size: 797 853 109 296 4072
Fragments arranged by size:
4072 1131 853 799 797 431 417 321
296 227 205 180 109
BbeI G_GCGC'C
Cuts at: 4580 4580
Size: 9838
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2277 5173 5743 6007 6060 6110 928
Size: 971 378 2896 570 264 53 50 4656
Fragments arranged by size:
4656 2896 971 570 378 264 53 50
BbvCI CC'TCA_GC
Cuts at: 2328 4329 4781 2328
Size: 2001 452 7385
Fragments arranged by size:
7385 2001 452
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8029 8063 1903
Size: 34 6092 34 3678
Fragments arranged by size:
6092 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6679 7730 8940 6679
Size: 1051 1210 7577
Fragments arranged by size:
7577 1210 1051
BclI T'GATC_A
Cuts at: 1548 4131 4267 6622 1548
Size: 2583 136 2355 4764
Fragments arranged by size:
4764 2583 2355 136
BfrBI ATG'CAT
Cuts at: 2663 3518 2663
Size: 855 8983
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2222 4585 5267
Size: 107 122 71 631 605 550 2363 682
Cuts at: 5267 8449 136
Size: 3182 1525
Fragments arranged by size:
3182 2363 1525 682 631 605 550 122
107 71
BglII A'GATC_T
Cuts at: 9833 9833
Size: 9838
BlpI GC'TnA_GC
Cuts at: 3727 5793 5867 7255 3727
Size: 2066 74 1388 6310
Fragments arranged by size:
6310 2066 1388 74
Bme1580I G_kGCm'C
Cuts at: 1790 2040 2262 2346 2646 2802 2970 3072 3957
Size: 250 222 84 300 156 168 102 885
Cuts at: 3957 4289 4650 6861 7899 9055 1790
Size: 332 361 2211 1038 1156 2573
Fragments arranged by size:
2573 2211 1156 1038 885 361 332 300
250 222 168 156 102 84
BmgBI CAC'GTC
Cuts at: 1318 5077 1318
Size: 3759 6079
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2687 2921 3711
Size: 416 53 83 399 24 1270 234 790
Cuts at: 3711 3750 4166 4560 4803 5213 5454 5569 5824
Size: 39 416 394 243 410 241 115 255
Cuts at: 5824 6039 7569 8527 442
Size: 215 1530 958 1753
Fragments arranged by size:
1753 1530 1270 958 790 416 416 410
399 394 255 243 241 234 215 115
83 53 39 24
BmtI G_CTAG'C
Cuts at: 1056 3488 1056
Size: 2432 7406
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2384 3856 4273 2384
Size: 1472 417 7949
Fragments arranged by size:
7949 1472 417
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2568 2766 2886 3027 3162 3587 4347 4618
Size: 1329 198 120 141 135 425 760 271
Cuts at: 4618 5086 5384 5565 5932 6314 6452 6530 6973
Size: 468 298 181 367 382 138 78 443
Cuts at: 6973 7041 8498 1239
Size: 68 1457 2579
Fragments arranged by size:
2579 1457 1329 760 468 443 425 382
367 298 271 198 181 141 138 135
120 78 68
Bpu10I CC'TnA_GC
Cuts at: 2328 2348 2758 3279 4329 4781 5025 5256 2328
Size: 20 410 521 1050 452 244 231 6910
Fragments arranged by size:
6910 1050 521 452 410 244 231 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2354 7963 8828 9126 2354
Size: 5609 865 298 3066
Fragments arranged by size:
5609 3066 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4002 6506 8501 882
Size: 617 2503 2504 1995 2219
Fragments arranged by size:
2504 2503 2219 1995 617
BsaAI yAC'GTr
Cuts at: 493 1204 4156 4299 7629 493
Size: 711 2952 143 3330 2702
Fragments arranged by size:
3330 2952 2702 711 143
BsaBI GATnn'nnATC
Cuts at: 6351 9832 6351
Size: 3481 6357
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4520 4577 7645 8027
Size: 53 83 186 936 2987 57 3068 382
Cuts at: 8027 8662 275
Size: 635 1451
Fragments arranged by size:
3068 2987 1451 936 635 382 186 83
57 53
BsaWI w'CCGG_w
Cuts at: 2058 3010 3142 3599 5424 5550 8271 8669 8943
Size: 952 132 457 1825 126 2721 398 274
Cuts at: 8943 9090 9501 2058
Size: 147 411 2395
Fragments arranged by size:
2721 2395 1825 952 457 411 398 274
147 132 126
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2541 2571 3784 3814 5561
Size: 30 165 30 1138 30 1213 30 1747
Cuts at: 5561 5591 7083 7113 1178
Size: 30 1492 30 3903
Fragments arranged by size:
3903 1747 1492 1213 1138 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 2126 2928 3792 3965 5786 5966 5969
Size: 913 27 802 864 173 1821 180 3
Cuts at: 5969 5972 5984 5987 6103 6794 7042 1186
Size: 3 12 3 116 691 248 3982
Fragments arranged by size:
3982 1821 913 864 802 691 248 180
173 116 27 12 3 3 3
BseYI C'CCAG_C
Cuts at: 2289 2309 3160 3615 5788 9041 9537 2289
Size: 20 851 455 2173 3253 496 2590
Fragments arranged by size:
3253 2590 2173 851 496 455 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3135 3213 3571 3606 3874 4110 1163
Size: 573 1399 78 358 35 268 236 6891
Fragments arranged by size:
6891 1399 573 358 268 236 78 35
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7288 8049 9077 1540
Size: 275 186 5287 761 1028 2301
Fragments arranged by size:
5287 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2040 2098 2190 3501 3728 3957 4106 4650 5523
Size: 58 92 1311 227 229 149 544 873
Cuts at: 5523 5672 6132 6438 6752 7899 7984 9055 2040
Size: 149 460 306 314 1147 85 1071 2823
Fragments arranged by size:
2823 1311 1147 1071 873 544 460 314
306 229 227 149 149 92 85 58
BsmI GAATG_Cn'
Cuts at: 2663 7349 7442 2663
Size: 4686 93 5059
Fragments arranged by size:
5059 4686 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2355 4527 6298 6759 2355
Size: 2172 1771 461 5434
Fragments arranged by size:
5434 2172 1771 461
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2098 2190 2262 2346 2370 2646
Size: 399 250 58 92 72 84 24 276
Cuts at: 2646 2802 2970 3072 3501 3728 3957 4049 4106
Size: 156 168 102 429 227 229 92 57
Cuts at: 4106 4254 4289 4650 4685 5102 5523 5672 5899
Size: 148 35 361 35 417 421 149 227
Cuts at: 5899 6132 6438 6752 6861 7157 7899 7984 9055
Size: 233 306 314 109 296 742 85 1071
Cuts at: 9055 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 429 421 417 399 361
314 306 296 276 250 233 229 227
227 168 156 149 148 109 102 92
92 85 84 72 58 57 35 35
24
BspEI T'CCGG_A
Cuts at: 5424 9501 5424
Size: 4077 5761
BspHI T'CATG_A
Cuts at: 6484 7727 9457 6484
Size: 1243 1730 6865
Fragments arranged by size:
6865 1730 1243
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3465 5019 5204 6970 7193 844
Size: 2621 1554 185 1766 223 3489
Fragments arranged by size:
3489 2621 1766 1554 223 185
BsrBI CCG'CTC
Cuts at: 1394 1823 2023 7725 1394
Size: 429 200 5702 3507
Fragments arranged by size:
5702 3507 429 200
BsrDI GCAATG_nn'
Cuts at: 65 1248 2218 3905 4033 4371 8333 8507 65
Size: 1183 970 1687 128 338 3962 174 1396
Fragments arranged by size:
3962 1687 1396 1183 970 338 174 128
BsrFI r'CCGG_y
Cuts at: 1700 1810 3142 4019 4432 8482 8669 1700
Size: 110 1332 877 413 4050 187 2869
Fragments arranged by size:
4050 2869 1332 877 413 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9838
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9838
BssSI C'ACGA_G
Cuts at: 5603 6472 7898 8910 5603
Size: 869 1426 1012 6531
Fragments arranged by size:
6531 1426 1012 869
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 9838
BstBI TT'CG_AA
Cuts at: 4837 8642 4837
Size: 3805 6033
BstEII G'GTnAC_C
Cuts at: 1184 2578 5776 1184
Size: 1394 3198 5246
Fragments arranged by size:
5246 3198 1394
BstXI CCAn_nnnn'nTGG
Cuts at: 2141 5419 6283 6586 2141
Size: 3278 864 303 5393
Fragments arranged by size:
5393 3278 864 303
BstYI r'GATC_y
Cuts at: 1972 2144 5137 6177 6634 6915 7037 7168 7535
Size: 172 2993 1040 457 281 122 131 367
Cuts at: 7535 7920 7937 9389 9475 9487 9833 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
2993 1977 1452 1040 457 385 367 346
281 172 131 122 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4281 4659 4854 6137 4281
Size: 378 195 1283 7982
Fragments arranged by size:
7982 1283 378 195
BtgI C'CryG_G
Cuts at: 513 1065 4425 4670 5418 6853 513
Size: 552 3360 245 748 1435 3498
Fragments arranged by size:
3498 3360 1435 748 552 245
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9099
Fragments arranged by size:
9099 683 56
BtsI GCAGTG_nn'
Cuts at: 2245 3398 6463 7354 8148 8168 2245
Size: 1153 3065 891 794 20 3915
Fragments arranged by size:
3915 3065 1153 891 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9838
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4579 4614 529
Size: 35 4015 35 5753
Fragments arranged by size:
5753 4015 35 35
DraI TTT'AAA
Cuts at: 6087 7142 6087
Size: 1055 8783
DraIII CAC_nnn'GTG
Cuts at: 3389 3389
Size: 9838
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8845 809
Size: 507 7529 1802
Fragments arranged by size:
7529 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3393 4164 4965
Size: 54 1425 50 275 18 1563 771 801
Cuts at: 4965 4995 6588 7285 8174 9803 8
Size: 30 1593 697 889 1629 43
Fragments arranged by size:
1629 1593 1563 1425 889 801 771 697
275 54 50 43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 7285 1537
Size: 275 5473 4090
Fragments arranged by size:
5473 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2086 5382 5763 5961 5981 6005 6213 6294
Size: 733 3296 381 198 20 24 208 81
Cuts at: 6294 6822 6887 7768 1353
Size: 528 65 881 3423
Fragments arranged by size:
3423 3296 881 733 528 381 208 198
81 65 24 20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6082 8425 8799 8945 387
Size: 1064 138 4493 2343 374 146 1280
Fragments arranged by size:
4493 2343 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2188 3499 3726 6750 2188
Size: 1311 227 3024 5276
Fragments arranged by size:
5276 3024 1311 227
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2568 2575 2766 2862 2886 2958 3027
Size: 393 936 7 191 96 24 72 69
Cuts at: 3027 3162 3232 3587 4347 4618 4946 5086 5265
Size: 135 70 355 760 271 328 140 179
Cuts at: 5265 5384 5487 5565 5932 6005 6314 6452 6530
Size: 119 103 78 367 73 309 138 78
Cuts at: 6530 6623 6920 6973 7041 7901 8498 9285 9553
Size: 93 297 53 68 860 597 787 268
Cuts at: 9553 1239
Size: 1524
Fragments arranged by size:
1524 936 860 787 760 597 393 367
355 328 309 297 271 268 191 179
140 138 135 119 103 96 93 78
78 73 72 70 69 68 53 24
7
EcoNI CCTnn'n_nnAGG
Cuts at: 6386 6386
Size: 9838
EcoO109I rG'GnC_Cy
Cuts at: 2332 2565 3634 3970 4096 4486 4702 4797 4850
Size: 233 1069 336 126 390 216 95 53
Cuts at: 4850 4904 5977 6858 2332
Size: 54 1073 881 5312
Fragments arranged by size:
5312 1073 1069 881 390 336 233 216
126 95 54 53
EcoRI G'AATT_C
Cuts at: 7118 7147 7525 7118
Size: 29 378 9431
Fragments arranged by size:
9431 378 29
FspI TGC'GCA
Cuts at: 6462 8344 6462
Size: 1882 7956
HaeII r_GCGC'y
Cuts at: 1678 2394 2789 4580 5116 6613 8985 1678
Size: 716 395 1791 536 1497 2372 2531
Fragments arranged by size:
2531 2372 1791 1497 716 536 395
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2099 2131 3826 3858 3863
Size: 32 135 32 527 32 1695 32 5
Cuts at: 3863 3895 4552 4584 5326 5358 5561 5593 6614
Size: 32 657 32 742 32 203 32 1021
Cuts at: 6614 6646 6739 6771 7022 7054 8479 8511 8553
Size: 32 93 32 251 32 1425 32 42
Cuts at: 8553 8585 1373
Size: 32 2626
Fragments arranged by size:
2626 1695 1425 1021 742 657 527 251
203 135 93 42 32 32 32 32
32 32 32 32 32 32 32 32
32 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2118 2461 3945 7182 7428 669
Size: 988 281 180 343 1484 3237 246 3079
Fragments arranged by size:
3237 3079 1484 988 343 281 246 180
HindIII A'AGCT_T
Cuts at: 748 2372 3899 6902 748
Size: 1624 1527 3003 3684
Fragments arranged by size:
3684 3003 1624 1527
HpaI GTT'AAC
Cuts at: 7428 7428
Size: 9838
KasI G'GCGC_C
Cuts at: 4576 4576
Size: 9838
KpnI G_GTAC'C
Cuts at: 7163 7163
Size: 9838
MfeI C'AATT_G
Cuts at: 7437 7437
Size: 9838
MluI A'CGCG_T
Cuts at: 9714 9714
Size: 9838
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2179 2488 3005 3107 3713 3912 6678 8952
Size: 874 309 517 102 606 199 2766 2274
Cuts at: 8952 9136 1305
Size: 184 2007
Fragments arranged by size:
2766 2274 2007 874 606 517 309 199
184 102
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3395 4166 4967 4997 6590
Size: 54 1425 343 1563 771 801 30 1593
Cuts at: 6590 10
Size: 3258
Fragments arranged by size:
3258 1593 1563 1425 801 771 343 54
30
MslI CAynn'nnrTG
Cuts at: 518 1166 2444 3850 3938 4109 4154 4838 4973
Size: 648 1278 1406 88 171 45 684 135
Cuts at: 4973 6953 7796 8155 8314 518
Size: 1980 843 359 159 2042
Fragments arranged by size:
2042 1980 1406 1278 843 684 648 359
171 159 135 88 45
MspA1I CmG'CkG
Cuts at: 1606 2031 3047 3212 3349 3823 4558 6064 6855
Size: 425 1016 165 137 474 735 1506 791
Cuts at: 6855 6997 7254 7931 9079 9324 1606
Size: 142 257 677 1148 245 2120
Fragments arranged by size:
2120 1506 1148 1016 791 735 677 474
425 257 245 165 142 137
NaeI GCC'GGC
Cuts at: 4434 4434
Size: 9838
NarI GG'CG_CC
Cuts at: 4577 4577
Size: 9838
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 9286
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9838
NgoMIV G'CCGG_C
Cuts at: 4432 4432
Size: 9838
NheI G'CTAG_C
Cuts at: 1052 3484 1052
Size: 2432 7406
NotI GC'GGCC_GC
Cuts at: 7285 7285
Size: 9838
NsiI A_TGCA'T
Cuts at: 2665 3520 2665
Size: 855 8983
NspI r_CATG'y
Cuts at: 1132 1229 2071 4978 7196 8741 1132
Size: 97 842 2907 2218 1545 2229
Fragments arranged by size:
2907 2229 2218 1545 842 97
PasI CC'CwG_GG
Cuts at: 1348 3638 4379 4909 5493 1348
Size: 2290 741 530 584 5693
Fragments arranged by size:
5693 2290 741 584 530
PciI A'CATG_T
Cuts at: 4974 8737 4974
Size: 3763 6075
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3349 3374 4676 4963 4987 5123 6124 1108
Size: 2241 25 1302 287 24 136 1001 4822
Fragments arranged by size:
4822 2241 1302 1001 287 136 25 24
PfoI T'CCnGG_A
Cuts at: 2423 5581 2423
Size: 3158 6680
PmeI GTTT'AAAC
Cuts at: 7142 7142
Size: 9838
PmlI CAC'GTG
Cuts at: 4299 4299
Size: 9838
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7907 7939 9420 9452 7907
Size: 32 1481 32 8293
Fragments arranged by size:
8293 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2565 3634 4096 4486 4702 4797 2565
Size: 1069 462 390 216 95 7606
Fragments arranged by size:
7606 1069 462 390 216 95
PshAI GACnn'nnGTC
Cuts at: 1613 3468 1613
Size: 1855 7983
PsiI TTA'TAA
Cuts at: 7408 7408
Size: 9838
PspOMI G'GGCC_C
Cuts at: 6857 6857
Size: 9838
PspXI vC'TCGA_Gb
Cuts at: 5271 6910 5271
Size: 1639 8199
PstI C_TGCA'G
Cuts at: 830 3277 3462 6458 7190 830
Size: 2447 185 2996 732 3478
Fragments arranged by size:
3478 2996 2447 732 185
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9838
PvuII CAG'CTG
Cuts at: 3212 3823 4558 6997 3212
Size: 611 735 2439 6053
Fragments arranged by size:
6053 2439 735 611
SacI G_AGCT'C
Cuts at: 2190 3501 3728 6752 2190
Size: 1311 227 3024 5276
Fragments arranged by size:
5276 3024 1311 227
SacII CC_GC'GG
Cuts at: 6856 6856
Size: 9838
SalI G'TCGA_C
Cuts at: 1936 7180 1936
Size: 5244 4594
SanDI GG'GwC_CC
Cuts at: 2565 3634 4096 4797 2565
Size: 1069 462 701 7606
Fragments arranged by size:
7606 1069 701 462
SapI GCTCTTCn'nnn_
Cuts at: 2086 5382 6005 6213 2086
Size: 3296 623 208 5711
Fragments arranged by size:
5711 3296 623 208
SbfI CC_TGCA'GG
Cuts at: 3462 7190 3462
Size: 3728 6110
ScaI AGT'ACT
Cuts at: 8086 9798 8086
Size: 1712 8126
SfcI C'TryA_G
Cuts at: 826 1045 2013 2026 2244 3273 3458 5701 6454
Size: 219 968 13 218 1029 185 2243 753
Cuts at: 6454 6973 7186 8321 9002 9193 826
Size: 519 213 1135 681 191 1471
Fragments arranged by size:
2243 1471 1135 1029 968 753 681 519
219 218 213 191 185 13
SfoI GGC'GCC
Cuts at: 4578 4578
Size: 9838
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9838
SgrAI Cr'CCGG_yG
Cuts at: 1700 3142 1700
Size: 1442 8396
SmaI CCC'GGG
Cuts at: 1872 2219 6401 7165 1872
Size: 347 4182 764 4545
Fragments arranged by size:
4545 4182 764 347
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2369 3806 5271 6910 7942 8843
Size: 197 926 426 1437 1465 1639 1032 901
Cuts at: 8843 9105 820
Size: 262 1553
Fragments arranged by size:
1639 1553 1465 1437 1032 926 901 426
262 197
SnaBI TAC'GTA
Cuts at: 493 7629 493
Size: 7136 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9838
SphI G_CATG'C
Cuts at: 1132 7196 1132
Size: 6064 3774
SspI AAT'ATT
Cuts at: 5 52 4441 7762 5
Size: 47 4389 3321 2081
Fragments arranged by size:
4389 3321 2081 47
StuI AGG'CCT
Cuts at: 1517 7018 1517
Size: 5501 4337
StyI C'CwwG_G
Cuts at: 513 1065 2178 2943 2985 3678 4401 5058 5412
Size: 552 1113 765 42 693 723 657 354
Cuts at: 5412 5616 6960 7102 7277 513
Size: 204 1344 142 175 3074
Fragments arranged by size:
3074 1344 1113 765 723 693 657 552
354 204 175 142 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2456 3169 3205 3247 3629 4099 4496
Size: 1255 876 713 36 42 382 470 397
Cuts at: 4496 5843 6425 7865 7882 8035 8220 8665 325
Size: 1347 582 1440 17 153 185 445 1498
Fragments arranged by size:
1498 1440 1347 1255 876 713 582 470
445 397 382 185 153 42 36 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 8084 9796 96
Size: 274 80 33 51 1443 6107 1712 138
Fragments arranged by size:
6107 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2541 2573 2722 2754 5118 5150 9798 9830 2541
Size: 32 149 32 2364 32 4648 32 2549
Fragments arranged by size:
4648 2549 2364 149 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9838
XbaI T'CTAG_A
Cuts at: 7174 7174
Size: 9838
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3013 4019 4916 3013
Size: 1006 897 7935
Fragments arranged by size:
7935 1006 897
XhoI C'TCGA_G
Cuts at: 1943 5271 6910 1943
Size: 3328 1639 4871
Fragments arranged by size:
4871 3328 1639
XmaI C'CCGG_G
Cuts at: 1870 2217 6399 7163 1870
Size: 347 4182 764 4545
Fragments arranged by size:
4545 4182 764 347
XmnI GAAnn'nnTTC
Cuts at: 2522 7967 2522
Size: 5445 4393
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7646 276
Size: 53 83 186 936 6112 2468
Fragments arranged by size:
6112 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BsiWI BstZ17I EcoRV FalI FseI
FspAI NruI PacI PsrI RsrII SexAI SfiI SrfI
SwaI