(Linear) (Six-base) MAPSORT of: KIEE3952.seq Check: 9928 from: 1 to: 5133 KIEE3952.orf With 182 enzymes: SgfI * March 22, 2012 16:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 3199 4965 5133 Size: 3199 1766 168 Fragments arranged by size: 3199 1766 168 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 570 857 953 1227 2941 3260 3482 4000 Size: 570 287 96 274 1714 319 222 518 Cuts at: 4000 4618 4915 5133 Size: 618 297 218 Fragments arranged by size: 1714 618 570 518 319 297 287 274 222 218 96 AfeI AGC'GCT Cuts at: 0 4606 5133 Size: 4606 527 AflII C'TTAA_G Cuts at: 0 1801 5133 Size: 1801 3332 AflIII A'CryG_T Cuts at: 0 2293 2969 5133 Size: 2293 676 2164 Fragments arranged by size: 2293 2164 676 AgeI A'CCGG_T Cuts at: 0 1137 5133 Size: 1137 3996 AhdI GACnn_n'nnGTC Cuts at: 0 2561 5133 Size: 2561 2572 AleI CACnn'nnGTG Cuts at: 0 1845 2104 2149 5133 Size: 1845 259 45 2984 Fragments arranged by size: 2984 1845 259 45 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1873 5133 Size: 1873 3260 AlwNI CAG_nnn'CTG Cuts at: 0 322 862 904 1344 1698 2173 2484 3006 Size: 322 540 42 440 354 475 311 522 Cuts at: 3006 3919 4995 5133 Size: 913 1076 138 Fragments arranged by size: 1076 913 540 522 475 440 354 322 311 138 42 ApaI G_GGCC'C Cuts at: 0 4856 5133 Size: 4856 277 ApaLI G'TGCA_C Cuts at: 0 31 1948 2641 5133 Size: 31 1917 693 2492 Fragments arranged by size: 2492 1917 693 31 ApoI r'AATT_y Cuts at: 0 4233 5113 5133 Size: 4233 880 20 Fragments arranged by size: 4233 880 20 AvaI C'yCGr_G Cuts at: 0 212 1332 1489 2071 2094 3266 3893 4394 Size: 212 1120 157 582 23 1172 627 501 Cuts at: 4394 4560 4905 5133 Size: 166 345 228 Fragments arranged by size: 1172 1120 627 582 501 345 228 212 166 157 23 AvrII C'CTAG_G Cuts at: 0 5097 5133 Size: 5097 36 BanI G'GyrC_C Cuts at: 0 336 846 1140 1278 1756 2143 2287 2387 Size: 336 510 294 138 478 387 144 100 Cuts at: 2387 2571 3878 5133 Size: 184 1307 1255 Fragments arranged by size: 1307 1255 510 478 387 336 294 184 144 138 100 BanII G_rGCy'C Cuts at: 0 185 365 1496 1723 2044 2249 2680 3097 Size: 185 180 1131 227 321 205 431 417 Cuts at: 3097 3894 4747 4856 5133 Size: 797 853 109 277 Fragments arranged by size: 1131 853 797 431 417 321 277 227 205 185 180 109 BbeI G_GCGC'C Cuts at: 0 2575 5133 Size: 2575 2558 BbsI GAAGACnn'nnnn_ Cuts at: 0 272 3168 3738 4002 4055 4105 5133 Size: 272 2896 570 264 53 50 1028 Fragments arranged by size: 2896 1028 570 272 264 53 50 BbvCI CC'TCA_GC Cuts at: 0 323 2324 2776 5133 Size: 323 2001 452 2357 Fragments arranged by size: 2357 2001 452 323 BciVI GTATCCnnnnn_n' Cuts at: 0 4674 5133 Size: 4674 459 BclI T'GATC_A Cuts at: 0 2126 2262 4617 5133 Size: 2126 136 2355 516 Fragments arranged by size: 2355 2126 516 136 BfrBI ATG'CAT Cuts at: 0 658 1513 5133 Size: 658 855 3620 Fragments arranged by size: 3620 855 658 BglI GCCn_nnn'nGGC Cuts at: 0 217 2580 3262 5133 Size: 217 2363 682 1871 Fragments arranged by size: 2363 1871 682 217 BlpI GC'TnA_GC Cuts at: 0 1722 3788 3862 5133 Size: 1722 2066 74 1271 Fragments arranged by size: 2066 1722 1271 74 Bme1580I G_kGCm'C Cuts at: 0 35 257 341 641 797 965 1067 1952 Size: 35 222 84 300 156 168 102 885 Cuts at: 1952 2284 2645 4856 5133 Size: 332 361 2211 277 Fragments arranged by size: 2211 885 361 332 300 277 222 168 156 102 84 35 BmgBI CAC'GTC Cuts at: 0 3072 5133 Size: 3072 2061 BmrI ACTGGGnnnn_n' Cuts at: 0 682 916 1706 1745 2161 2555 2798 3208 Size: 682 234 790 39 416 394 243 410 Cuts at: 3208 3449 3564 3819 4034 5133 Size: 241 115 255 215 1099 Fragments arranged by size: 1099 790 682 416 410 394 255 243 241 234 215 115 39 BmtI G_CTAG'C Cuts at: 0 1483 5133 Size: 1483 3650 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 379 1851 2268 5133 Size: 379 1472 417 2865 Fragments arranged by size: 2865 1472 417 379 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 563 761 881 1022 1157 1582 2342 2613 Size: 563 198 120 141 135 425 760 271 Cuts at: 2613 3081 3379 3560 3927 4309 4447 4525 4968 Size: 468 298 181 367 382 138 78 443 Cuts at: 4968 5036 5133 Size: 68 97 Fragments arranged by size: 760 563 468 443 425 382 367 298 271 198 181 141 138 135 120 97 78 68 Bpu10I CC'TnA_GC Cuts at: 0 323 343 753 1274 2324 2776 3020 3251 Size: 323 20 410 521 1050 452 244 231 Cuts at: 3251 5133 Size: 1882 Fragments arranged by size: 1882 1050 521 452 410 323 244 231 20 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 349 5133 Size: 349 4784 BsaI GGTCTCn'nnnn_ Cuts at: 0 1997 4501 5133 Size: 1997 2504 632 Fragments arranged by size: 2504 1997 632 BsaAI yAC'GTr Cuts at: 0 2151 2294 5133 Size: 2151 143 2839 Fragments arranged by size: 2839 2151 143 BsaBI GATnn'nnATC Cuts at: 0 4346 5133 Size: 4346 787 BsaHI Gr'CG_yC Cuts at: 0 2515 2572 5133 Size: 2515 57 2561 Fragments arranged by size: 2561 2515 57 BsaWI w'CCGG_w Cuts at: 0 53 1005 1137 1594 3419 3545 5133 Size: 53 952 132 457 1825 126 1588 Fragments arranged by size: 1825 1588 952 457 132 126 53 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 536 566 1779 1809 3556 3586 5078 5108 Size: 536 30 1213 30 1747 30 1492 30 Cuts at: 5108 5133 Size: 25 Fragments arranged by size: 1747 1492 1213 536 30 30 30 30 25 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 94 121 923 1787 1960 3781 3961 3964 Size: 94 27 802 864 173 1821 180 3 Cuts at: 3964 3967 3979 3982 4098 4789 5037 5133 Size: 3 12 3 116 691 248 96 Fragments arranged by size: 1821 864 802 691 248 180 173 116 96 94 27 12 3 3 3 BseYI C'CCAG_C Cuts at: 0 284 304 1155 1610 3783 5133 Size: 284 20 851 455 2173 1350 Fragments arranged by size: 2173 1350 851 455 284 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1130 1208 1566 1601 1869 2105 5133 Size: 1130 78 358 35 268 236 3028 Fragments arranged by size: 3028 1130 358 268 236 78 35 BsiHKAI G_wGCw'C Cuts at: 0 35 93 185 1496 1723 1952 2101 2645 Size: 35 58 92 1311 227 229 149 544 Cuts at: 2645 3518 3667 4127 4433 4747 5133 Size: 873 149 460 306 314 386 Fragments arranged by size: 1311 873 544 460 386 314 306 229 227 149 149 92 58 35 BsmI GAATG_Cn' Cuts at: 0 658 5133 Size: 658 4475 BsmBI CGTCTCn'nnnn_ Cuts at: 0 350 2522 4293 4754 5133 Size: 350 2172 1771 461 379 Fragments arranged by size: 2172 1771 461 379 350 Bsp1286I G_dGCh'C Cuts at: 0 35 93 185 257 341 365 641 797 Size: 35 58 92 72 84 24 276 156 Cuts at: 797 965 1067 1496 1723 1952 2044 2101 2249 Size: 168 102 429 227 229 92 57 148 Cuts at: 2249 2284 2645 2680 3097 3518 3667 3894 4127 Size: 35 361 35 417 421 149 227 233 Cuts at: 4127 4433 4747 4856 5133 Size: 306 314 109 277 Fragments arranged by size: 429 421 417 361 314 306 277 276 233 229 227 227 168 156 149 148 109 102 92 92 84 72 58 57 35 35 35 24 BspEI T'CCGG_A Cuts at: 0 3419 5133 Size: 3419 1714 BspHI T'CATG_A Cuts at: 0 4479 5133 Size: 4479 654 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1460 3014 3199 4965 5133 Size: 1460 1554 185 1766 168 Fragments arranged by size: 1766 1554 1460 185 168 BsrBI CCG'CTC Cuts at: 0 18 5133 Size: 18 5115 BsrDI GCAATG_nn' Cuts at: 0 213 1900 2028 2366 5133 Size: 213 1687 128 338 2767 Fragments arranged by size: 2767 1687 338 213 128 BsrFI r'CCGG_y Cuts at: 0 1137 2014 2427 5133 Size: 1137 877 413 2706 Fragments arranged by size: 2706 1137 877 413 BssSI C'ACGA_G Cuts at: 0 3598 4467 5133 Size: 3598 869 666 Fragments arranged by size: 3598 869 666 BstBI TT'CG_AA Cuts at: 0 2832 5133 Size: 2832 2301 BstEII G'GTnAC_C Cuts at: 0 573 3771 5133 Size: 573 3198 1362 Fragments arranged by size: 3198 1362 573 BstXI CCAn_nnnn'nTGG Cuts at: 0 136 3414 4278 4581 5133 Size: 136 3278 864 303 552 Fragments arranged by size: 3278 864 552 303 136 BstYI r'GATC_y Cuts at: 0 139 3132 4172 4629 4910 5032 5133 Size: 139 2993 1040 457 281 122 101 Fragments arranged by size: 2993 1040 457 281 139 122 101 Bsu36I CC'TnA_GG Cuts at: 0 2276 2654 2849 4132 5133 Size: 2276 378 195 1283 1001 Fragments arranged by size: 2276 1283 1001 378 195 BtgI C'CryG_G Cuts at: 0 2420 2665 3413 4848 5133 Size: 2420 245 748 1435 285 Fragments arranged by size: 2420 1435 748 285 245 BtsI GCAGTG_nn' Cuts at: 0 240 1393 4458 5133 Size: 240 1153 3065 675 Fragments arranged by size: 3065 1153 675 240 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2574 2609 5133 Size: 2574 35 2524 Fragments arranged by size: 2574 2524 35 DraI TTT'AAA Cuts at: 0 4082 5133 Size: 4082 1051 DraIII CAC_nnn'GTG Cuts at: 0 1384 5133 Size: 1384 3749 EaeI y'GGCC_r Cuts at: 0 1388 2159 2960 2990 4583 5133 Size: 1388 771 801 30 1593 550 Fragments arranged by size: 1593 1388 801 771 550 30 EarI CTCTTCn'nnn_ Cuts at: 0 81 3377 3758 3956 3976 4000 4208 4289 Size: 81 3296 381 198 20 24 208 81 Cuts at: 4289 4817 4882 5133 Size: 528 65 251 Fragments arranged by size: 3296 528 381 251 208 198 81 81 65 24 20 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 4077 5133 Size: 4077 1056 EcoICRI GAG'CTC Cuts at: 0 183 1494 1721 4745 5133 Size: 183 1311 227 3024 388 Fragments arranged by size: 3024 1311 388 227 183 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 563 570 761 857 881 953 1022 1157 Size: 563 7 191 96 24 72 69 135 Cuts at: 1157 1227 1582 2342 2613 2941 3081 3260 3379 Size: 70 355 760 271 328 140 179 119 Cuts at: 3379 3482 3560 3927 4000 4309 4447 4525 4618 Size: 103 78 367 73 309 138 78 93 Cuts at: 4618 4915 4968 5036 5133 Size: 297 53 68 97 Fragments arranged by size: 760 563 367 355 328 309 297 271 191 179 140 138 135 119 103 97 96 93 78 78 73 72 70 69 68 53 24 7 EcoNI CCTnn'n_nnAGG Cuts at: 0 4381 5133 Size: 4381 752 EcoO109I rG'GnC_Cy Cuts at: 0 327 560 1629 1965 2091 2481 2697 2792 Size: 327 233 1069 336 126 390 216 95 Cuts at: 2792 2845 2899 3972 4853 5133 Size: 53 54 1073 881 280 Fragments arranged by size: 1073 1069 881 390 336 327 280 233 216 126 95 54 53 EcoRI G'AATT_C Cuts at: 0 5113 5133 Size: 5113 20 FspI TGC'GCA Cuts at: 0 4457 5133 Size: 4457 676 HaeII r_GCGC'y Cuts at: 0 389 784 2575 3111 4608 5133 Size: 389 395 1791 536 1497 525 Fragments arranged by size: 1791 1497 536 525 395 389 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 94 126 1821 1853 1858 1890 2547 2579 Size: 94 32 1695 32 5 32 657 32 Cuts at: 2579 3321 3353 3556 3588 4609 4641 4734 4766 Size: 742 32 203 32 1021 32 93 32 Cuts at: 4766 5017 5049 5133 Size: 251 32 84 Fragments arranged by size: 1695 1021 742 657 251 203 94 93 84 32 32 32 32 32 32 32 32 32 5 HincII GTy'rAC Cuts at: 0 113 456 1940 5133 Size: 113 343 1484 3193 Fragments arranged by size: 3193 1484 343 113 HindIII A'AGCT_T Cuts at: 0 367 1894 4897 5133 Size: 367 1527 3003 236 Fragments arranged by size: 3003 1527 367 236 KasI G'GCGC_C Cuts at: 0 2571 5133 Size: 2571 2562 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 174 483 1000 1102 1708 1907 4673 5133 Size: 174 309 517 102 606 199 2766 460 Fragments arranged by size: 2766 606 517 460 309 199 174 102 MscI TGG'CCA Cuts at: 0 1390 2161 2962 2992 4585 5133 Size: 1390 771 801 30 1593 548 Fragments arranged by size: 1593 1390 801 771 548 30 MslI CAynn'nnrTG Cuts at: 0 439 1845 1933 2104 2149 2833 2968 4948 Size: 439 1406 88 171 45 684 135 1980 Cuts at: 4948 5133 Size: 185 Fragments arranged by size: 1980 1406 684 439 185 171 135 88 45 MspA1I CmG'CkG Cuts at: 0 26 1042 1207 1344 1818 2553 4059 4850 Size: 26 1016 165 137 474 735 1506 791 Cuts at: 4850 4992 5133 Size: 142 141 Fragments arranged by size: 1506 1016 791 735 474 165 142 141 137 26 NaeI GCC'GGC Cuts at: 0 2429 5133 Size: 2429 2704 NarI GG'CG_CC Cuts at: 0 2572 5133 Size: 2572 2561 NgoMIV G'CCGG_C Cuts at: 0 2427 5133 Size: 2427 2706 NheI G'CTAG_C Cuts at: 0 1479 5133 Size: 1479 3654 NsiI A_TGCA'T Cuts at: 0 660 1515 5133 Size: 660 855 3618 Fragments arranged by size: 3618 855 660 NspI r_CATG'y Cuts at: 0 66 2973 5133 Size: 66 2907 2160 Fragments arranged by size: 2907 2160 66 PasI CC'CwG_GG Cuts at: 0 1633 2374 2904 3488 5133 Size: 1633 741 530 584 1645 Fragments arranged by size: 1645 1633 741 584 530 PciI A'CATG_T Cuts at: 0 2969 5133 Size: 2969 2164 PflMI CCAn_nnn'nTGG Cuts at: 0 1344 1369 2671 2958 2982 3118 4119 5133 Size: 1344 25 1302 287 24 136 1001 1014 Fragments arranged by size: 1344 1302 1014 1001 287 136 25 24 PfoI T'CCnGG_A Cuts at: 0 418 3576 5133 Size: 418 3158 1557 Fragments arranged by size: 3158 1557 418 PmlI CAC'GTG Cuts at: 0 2294 5133 Size: 2294 2839 PpuMI rG'GwC_Cy Cuts at: 0 560 1629 2091 2481 2697 2792 5133 Size: 560 1069 462 390 216 95 2341 Fragments arranged by size: 2341 1069 560 462 390 216 95 PshAI GACnn'nnGTC Cuts at: 0 1463 5133 Size: 1463 3670 PspOMI G'GGCC_C Cuts at: 0 4852 5133 Size: 4852 281 PspXI vC'TCGA_Gb Cuts at: 0 3266 4905 5133 Size: 3266 1639 228 Fragments arranged by size: 3266 1639 228 PstI C_TGCA'G Cuts at: 0 1272 1457 4453 5133 Size: 1272 185 2996 680 Fragments arranged by size: 2996 1272 680 185 PvuII CAG'CTG Cuts at: 0 1207 1818 2553 4992 5133 Size: 1207 611 735 2439 141 Fragments arranged by size: 2439 1207 735 611 141 SacI G_AGCT'C Cuts at: 0 185 1496 1723 4747 5133 Size: 185 1311 227 3024 386 Fragments arranged by size: 3024 1311 386 227 185 SacII CC_GC'GG Cuts at: 0 4851 5133 Size: 4851 282 SanDI GG'GwC_CC Cuts at: 0 560 1629 2091 2792 5133 Size: 560 1069 462 701 2341 Fragments arranged by size: 2341 1069 701 560 462 SapI GCTCTTCn'nnn_ Cuts at: 0 81 3377 4000 4208 5133 Size: 81 3296 623 208 925 Fragments arranged by size: 3296 925 623 208 81 SbfI CC_TGCA'GG Cuts at: 0 1457 5133 Size: 1457 3676 SfcI C'TryA_G Cuts at: 0 8 21 239 1268 1453 3696 4449 4968 Size: 8 13 218 1029 185 2243 753 519 Cuts at: 4968 5133 Size: 165 Fragments arranged by size: 2243 1029 753 519 218 185 165 13 8 SfoI GGC'GCC Cuts at: 0 2573 5133 Size: 2573 2560 SgrAI Cr'CCGG_yG Cuts at: 0 1137 5133 Size: 1137 3996 SmaI CCC'GGG Cuts at: 0 214 4396 5133 Size: 214 4182 737 Fragments arranged by size: 4182 737 214 SmlI C'TyrA_G Cuts at: 0 364 1801 3266 4905 5133 Size: 364 1437 1465 1639 228 Fragments arranged by size: 1639 1465 1437 364 228 SspI AAT'ATT Cuts at: 0 2436 5133 Size: 2436 2697 StuI AGG'CCT Cuts at: 0 5013 5133 Size: 5013 120 StyI C'CwwG_G Cuts at: 0 173 938 980 1673 2396 3053 3407 3611 Size: 173 765 42 693 723 657 354 204 Cuts at: 3611 4955 5097 5133 Size: 1344 142 36 Fragments arranged by size: 1344 765 723 693 657 354 204 173 142 42 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 451 1164 1200 1242 1624 2094 2491 3838 Size: 451 713 36 42 382 470 397 1347 Cuts at: 3838 4420 5133 Size: 582 713 Fragments arranged by size: 1347 713 713 582 470 451 397 382 42 36 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 536 568 717 749 3113 3145 5133 Size: 536 32 149 32 2364 32 1988 Fragments arranged by size: 2364 1988 536 149 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1008 2014 2911 5133 Size: 1008 1006 897 2222 Fragments arranged by size: 2222 1008 1006 897 XhoI C'TCGA_G Cuts at: 0 3266 4905 5133 Size: 3266 1639 228 Fragments arranged by size: 3266 1639 228 XmaI C'CCGG_G Cuts at: 0 212 4394 5133 Size: 212 4182 739 Fragments arranged by size: 4182 739 212 XmnI GAAnn'nnTTC Cuts at: 0 517 5133 Size: 517 4616 Enzymes that do cut: AarI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AvaI AvrII BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstBI BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI DraI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI NsiI NspI PasI PciI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI PstI PvuII SacI SacII SanDI SapI SbfI SfcI SfoI SgrAI SmaI SmlI SspI StuI StyI TaqII TstI XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AloI AscI AseI AsiSI BaeI BamHI BcgI BglII BsiEI BsiWI BsrGI BssHII BstAPI BstZ17I BtgZI ClaI DrdI EagI EcoRV FalI FseI FspAI HpaI KpnI MfeI MluI NcoI NdeI NotI NruI PacI PmeI PpiI PsiI PsrI PvuI RsrII SalI ScaI SexAI SfiI SgfI SnaBI SpeI SphI SrfI SwaI TatI Tth111I XbaI ZraI