################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaII AviII AvrII BanI BbeI BbrPI BbvCI BcgI BclI BcnI BfrI BglI BlnI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmFI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I Bsu36I CfoI Cfr10I Cfr13I CpoI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII KasI KpnI KspI MamI MluI MroI MspI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI Sau96I SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccII 810 CGCG CG^CG AflIII 1786 ACACGT A^C[GA][CT]GT AlwNI 529 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 1596 GAGCTC G[AT]GC[AT]^C AvaI 441 CCCGAG C^[CT]CG[GA]G BamHI 237 GGATCC G^GATCC BanII 1596 GAGCTC G[GA]GC[CT]^C BciVI 662 GTATCC GTATCC(6/5) BlpI 988 GCTGAGC GC^T[ACGT]AGC BmyI 1596 GAGCTC G[AGT]GC[ACT]^C Bpu1102I 988 GCTGAGC GC^T[ACGT]AGC BsgI 980 CTGCAC GTGCAG(16/14) BsiHKAI 1596 GAGCTC G[AT]GC[AT]^C BsmI 967 GAATGC GAATGC(1/-1) BsoBI 441 CCCGAG C^[CT]CG[GA]G Bsp1286I 1596 GAGCTC G[AGT]GC[ACT]^C BspDI 97 ATCGAT AT^CGAT BspMI 115 GCAGGT ACCTGC(4/8) BstEII 868 GGTTACC G^GT[ACGT]ACC BstPI 868 GGTTACC G^GT[ACGT]ACC BstUI 810 CGCG CG^CG BstXI 1601 CCATCAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 988 GCTGAGC GC^T[ACGT]AGC ClaI 97 ATCGAT AT^CGAT DraI 1791 TTTAAA TTT^AAA DsaI 956 CCACGG C^C[GA][CT]GG EcoNI 51 CCTTCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 868 GGTTACC G^GT[ACGT]ACC FauI 12 GCGGG CCCGC(4/6) HindIII 1538 AAGCTT A^AGCTT MfeI 1637 CAATTG C^AATTG MslI 1124 CATCTGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 10 CAGCGG C[AC]G^C[GT]G MunI 1637 CAATTG C^AATTG MvnI 810 CGCG CG^CG NdeI 1701 CATATG CA^TATG PshAI 121 GACAAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 1596 GAGCTC GAGCT^C ScaI 179 AGTACT AGT^ACT SfcI 1090 CTACAG C^T[GA][CT]AG SspI 689 AATATT AAT^ATT ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### Asp700I 87 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1004 GAAATCATTC BalI 326 TGGCCA TGG^CCA 1314 TGGCCA BbsI 813 GAAGAC GAAGAC(2/6) 128 GTCTTC BbvI 937 GCAGC GCAGC(8/12) 1270 GCTGC BglII 72 AGATCT A^GATCT 1428 AGATCT BpmI 742 CTCCAG CTGGAG(16/14) 1663 CTGGAG BpuAI 813 GAAGAC GAAGAC(2/6) 128 GTCTTC BsaJI 886 CCCAGG C^C[ACGT][ACGT]GG 956 CCACGG Bsp1407I 486 TGTACA T^GTACA 1381 TGTACA BsrGI 486 TGTACA T^GTACA 1381 TGTACA EaeI 326 TGGCCA [CT]^GGCC[GA] 1314 TGGCCA HaeIII 327 GGCC GG^CC 1315 GGCC MaeII 1755 ACGT A^CGT 1788 ACGT MluNI 326 TGGCCA TGG^CCA 1314 TGGCCA MscI 326 TGGCCA TGG^CCA 1314 TGGCCA NlaIV 237 GGATCC GG[ACGT]^[ACGT]CC 1434 GGCTCC PleI 446 GACTC GAGTC(4/5) 1666 GAGTC SfaNI 769 GATGC GCATC(5/9) 1123 GCATC SspBI 486 TGTACA T^GTACA 1381 TGTACA TaqI 98 TCGA T^CGA 1112 TCGA TseI 937 GCAGC G^C[AT]GC 1270 GCTGC Tsp45I 119 GTGAC ^GT[GC]AC 1507 GTCAC TthHB8I 98 TCGA T^CGA 1112 TCGA XmnI 87 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1004 GAAATCATTC ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI BfaI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI BstYI Cac8I DdeI DpnII EarI EcoRII Fnu4HI FokI HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII RsaI Sau3AI ScrFI TfiI Tru9I Tsp509I TspRI XhoII