Restriction map for
pFN21AE3945
(Circular) (Six-base) MAPSORT of: pFN21AE3945.seq Check: 4792 from: 1 to: 6523
pFN21AE3945 6523 bp
With 182 enzymes: SgfI *
March 28, 2012 11:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2111 2111
Size: 6523
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4333 278
Size: 53 83 186 936 2797 2468
Fragments arranged by size:
2797 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3866 1937
Size: 1929 4594
Acc65I G'GTAC_C
Cuts at: 3844 3844
Size: 6523
AclI AA'CG_TT
Cuts at: 1563 4650 5023 1563
Size: 3087 373 3063
Fragments arranged by size:
3087 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2045 2366 3144 3519 3537 3714 3753 4586
Size: 413 321 778 375 18 177 39 833
Cuts at: 4586 5970 6238 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 833 778 413 375 321 268
177 39 18
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6326
AflIII A'CryG_T
Cuts at: 1203 3791 5422 6399 1203
Size: 2588 1631 977 1327
Fragments arranged by size:
2588 1631 1327 977
AgeI A'CCGG_T
Cuts at: 5354 5354
Size: 6523
AhdI GACnn_n'nnGTC
Cuts at: 5252 5252
Size: 6523
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6523
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6523
AlwNI CAG_nnn'CTG
Cuts at: 2539 4266 5838 2539
Size: 1727 1572 3224
Fragments arranged by size:
3224 1727 1572
ApaLI G'TGCA_C
Cuts at: 4580 5736 4580
Size: 1156 5367
ApoI r'AATT_y
Cuts at: 1493 2096 2471 3450 3584 3680 3832 4058 4210
Size: 603 375 979 134 96 152 226 152
Cuts at: 4210 6319 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 979 603 375 226 152 152
134 96
AseI AT'TA_AT
Cuts at: 160 1032 5077 160
Size: 872 4045 1606
Fragments arranged by size:
4045 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6523
AvaI C'yCGr_G
Cuts at: 1870 1943 2446 3848 1870
Size: 73 503 1402 4545
Fragments arranged by size:
4545 1402 503 73
BamHI G'GATC_C
Cuts at: 2242 3853 4220 2242
Size: 1611 367 4545
Fragments arranged by size:
4545 1611 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3844 5299 618
Size: 325 217 627 2057 1455 1842
Fragments arranged by size:
2057 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3605 3842 1391
Size: 2214 237 4072
Fragments arranged by size:
4072 2214 237
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2126 2825 928
Size: 971 227 699 4626
Fragments arranged by size:
4626 971 699 227
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4714 4748 1903
Size: 34 2777 34 3678
Fragments arranged by size:
3678 2777 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2678 4415 5625 2678
Size: 1737 1210 3576
Fragments arranged by size:
3576 1737 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6523
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5134 136
Size: 107 122 71 631 605 3462 1525
Fragments arranged by size:
3462 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2077 3433 6518 2077
Size: 1356 3085 2082
Fragments arranged by size:
3085 2082 1356
BlpI GC'TnA_GC
Cuts at: 2994 3940 2994
Size: 946 5577
Bme1580I G_kGCm'C
Cuts at: 1790 4584 5740 1790
Size: 2794 1156 2573
Fragments arranged by size:
2794 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6523
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2832 4254 5212
Size: 416 53 83 399 24 1415 1422 958
Cuts at: 5212 442
Size: 1753
Fragments arranged by size:
1753 1422 1415 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6523
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2732 3689 5183 1239
Size: 1493 957 1494 2579
Fragments arranged by size:
2579 1494 1493 957
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4648 5513 5811 4648
Size: 865 298 5360
Fragments arranged by size:
5360 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5186 882
Size: 617 3687 2219
Fragments arranged by size:
3687 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4314 493
Size: 711 3110 2702
Fragments arranged by size:
3110 2702 711
BsaBI GATnn'nnATC
Cuts at: 6517 6517
Size: 6523
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4330 4712 5347 275
Size: 53 83 186 936 2797 382 635 1451
Fragments arranged by size:
2797 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4956 5354 5628 5775 6186 4956
Size: 398 274 147 411 5293
Fragments arranged by size:
5293 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3462 3492 1178
Size: 30 165 30 2059 30 4209
Fragments arranged by size:
4209 2059 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6523
BseYI C'CCAG_C
Cuts at: 2013 3030 3740 5726 6222 2013
Size: 1017 710 1986 496 2314
Fragments arranged by size:
2314 1986 1017 710 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2970 1163
Size: 573 1234 4716
Fragments arranged by size:
4716 1234 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3973 4734 5762 1540
Size: 275 186 1972 761 1028 2301
Fragments arranged by size:
2301 1972 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3605 4584 4669 5740 3605
Size: 979 85 1071 4388
Fragments arranged by size:
4388 1071 979 85
BsmI GAATG_Cn'
Cuts at: 2978 4034 4127 2978
Size: 1056 93 5374
Fragments arranged by size:
5374 1056 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3605 3842 4584 4669 5740 1391
Size: 399 1815 237 742 85 1071 2174
Fragments arranged by size:
2174 1815 1071 742 399 237 85
BspEI T'CCGG_A
Cuts at: 6186 6186
Size: 6523
BspHI T'CATG_A
Cuts at: 4412 6142 4412
Size: 1730 4793
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2111 3878 844
Size: 1267 1767 3489
Fragments arranged by size:
3489 1767 1267
BsrBI CCG'CTC
Cuts at: 1394 1823 4410 1394
Size: 429 2587 3507
Fragments arranged by size:
3507 2587 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5018 5192 65
Size: 1183 3770 174 1396
Fragments arranged by size:
3770 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5167 5354 1700
Size: 110 3357 187 2869
Fragments arranged by size:
3357 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2491 3386 96
Size: 2395 895 3233
Fragments arranged by size:
3233 2395 895
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6523
BssSI C'ACGA_G
Cuts at: 4583 5595 4583
Size: 1012 5511
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6523
BstBI TT'CG_AA
Cuts at: 5327 5327
Size: 6523
BstEII G'GTnAC_C
Cuts at: 1184 2873 1184
Size: 1689 4834
BstXI CCAn_nnnn'nTGG
Cuts at: 3613 3613
Size: 6523
BstYI r'GATC_y
Cuts at: 1972 2077 2242 2346 3433 3629 3853 4220 4605
Size: 105 165 104 1087 196 224 367 385
Cuts at: 4605 4622 6074 6160 6172 6518 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1087 385 367 346 224 196
165 105 104 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2961 513
Size: 552 939 957 4075
Fragments arranged by size:
4075 957 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5784
Fragments arranged by size:
5784 683 56
BtsI GCAGTG_nn'
Cuts at: 2607 2952 4039 4833 4853 2607
Size: 345 1087 794 20 4277
Fragments arranged by size:
4277 1087 794 345 20
ClaI AT'CG_AT
Cuts at: 1554 2103 1554
Size: 549 5974
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6488
DraI TTT'AAA
Cuts at: 3798 3827 3798
Size: 29 6494
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5530 809
Size: 507 4214 1802
Fragments arranged by size:
4214 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2331 3319 3970
Size: 54 1425 50 275 18 501 988 651
Cuts at: 3970 4859 6488 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 988 889 651 501 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3970 1537
Size: 275 2158 4090
Fragments arranged by size:
4090 2158 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2502 3179 3809 4453 1353
Size: 1149 677 630 644 3423
Fragments arranged by size:
3423 1149 677 644 630
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5110 5484 5630 387
Size: 1064 138 3521 374 146 1280
Fragments arranged by size:
3521 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3603 3603
Size: 6523
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2366 2732 3144 3519 3537 3689
Size: 393 413 321 366 412 375 18 152
Cuts at: 3689 3714 3753 4586 5183 5970 6238 1239
Size: 25 39 833 597 787 268 1524
Fragments arranged by size:
1524 833 787 597 413 412 393 375
366 321 268 152 39 25 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2060 2060
Size: 6523
EcoRI G'AATT_C
Cuts at: 3832 4210 3832
Size: 378 6145
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2154 2882 3542 2154
Size: 728 660 5135
Fragments arranged by size:
5135 728 660
FspI TGC'GCA
Cuts at: 5029 5029
Size: 6523
HaeII r_GCGC'y
Cuts at: 1678 5670 1678
Size: 3992 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3340 3372 3462 3494 5164
Size: 32 135 32 1768 32 90 32 1670
Cuts at: 5164 5196 5238 5270 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1768 1670 135 90 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3867 4113 669
Size: 988 281 1929 246 3079
Fragments arranged by size:
3079 1929 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3543 748
Size: 2795 3728
HpaI GTT'AAC
Cuts at: 4113 4113
Size: 6523
KpnI G_GTAC'C
Cuts at: 3848 3848
Size: 6523
MfeI C'AATT_G
Cuts at: 3642 4122 3642
Size: 480 6043
MluI A'CGCG_T
Cuts at: 6399 6399
Size: 6523
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3121 5637 5821 1305
Size: 1816 2516 184 2007
Fragments arranged by size:
2516 2007 1816 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2333 3321 10
Size: 54 1425 343 501 988 3212
Fragments arranged by size:
3212 1425 988 501 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3133 4481 4840 4999 518
Size: 648 1967 1348 359 159 2042
Fragments arranged by size:
2042 1967 1348 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2017 3939 4616 5764 6009 1606
Size: 411 1922 677 1148 245 2120
Fragments arranged by size:
2120 1922 1148 677 411 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5032
Fragments arranged by size:
5032 939 552
NdeI CA'TA_TG
Cuts at: 387 3707 387
Size: 3320 3203
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6523
NotI GC'GGCC_GC
Cuts at: 3970 3970
Size: 6523
NspI r_CATG'y
Cuts at: 1132 1229 3881 5426 1132
Size: 97 2652 1545 2229
Fragments arranged by size:
2652 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6523
PciI A'CATG_T
Cuts at: 5422 5422
Size: 6523
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6523
PfoI T'CCnGG_A
Cuts at: 2082 2082
Size: 6523
PmeI GTTT'AAAC
Cuts at: 3827 3827
Size: 6523
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4592 4624 6105 6137 4592
Size: 32 1481 32 4978
Fragments arranged by size:
4978 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2130 1613
Size: 517 6006
PsiI TTA'TAA
Cuts at: 4093 4093
Size: 6523
PstI C_TGCA'G
Cuts at: 830 3875 830
Size: 3045 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6523
SacI G_AGCT'C
Cuts at: 3605 3605
Size: 6523
SalI G'TCGA_C
Cuts at: 1936 3865 1936
Size: 1929 4594
SbfI CC_TGCA'GG
Cuts at: 3875 3875
Size: 6523
ScaI AGT'ACT
Cuts at: 2186 4771 6483 2186
Size: 2585 1712 2226
Fragments arranged by size:
2585 2226 1712
SfcI C'TryA_G
Cuts at: 826 1045 3095 3871 5006 5687 5878 826
Size: 219 2050 776 1135 681 191 1471
Fragments arranged by size:
2050 1471 1135 776 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6523
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6523
SmaI CCC'GGG
Cuts at: 1872 3850 1872
Size: 1978 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4627 5528 5790 820
Size: 197 926 2684 901 262 1553
Fragments arranged by size:
2684 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4314 493
Size: 3821 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6523
SphI G_CATG'C
Cuts at: 1132 3881 1132
Size: 2749 3774
SspI AAT'ATT
Cuts at: 5 52 2696 4447 5
Size: 47 2644 1751 2081
Fragments arranged by size:
2644 2081 1751 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6523
StyI C'CwwG_G
Cuts at: 513 1065 2004 3962 513
Size: 552 939 1958 3074
Fragments arranged by size:
3074 1958 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2602 3167 3596 3703 4550 4567 4720
Size: 1255 1022 565 429 107 847 17 153
Cuts at: 4720 4905 5350 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1022 847 565 445 429 185
153 107 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2184 2277 2491
Size: 274 80 33 51 1443 207 93 214
Cuts at: 2491 3386 4769 6481 96
Size: 895 1383 1712 138
Fragments arranged by size:
1712 1443 1383 895 274 214 207 138
93 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3051 3083 6483 6515 3051
Size: 32 3400 32 3059
Fragments arranged by size:
3400 3059 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6523
XbaI T'CTAG_A
Cuts at: 3859 3859
Size: 6523
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6523
XmaI C'CCGG_G
Cuts at: 1870 3848 1870
Size: 1978 4545
XmnI GAAnn'nnTTC
Cuts at: 2096 3013 4652 2096
Size: 917 1639 3967
Fragments arranged by size:
3967 1639 917
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4331 276
Size: 53 83 186 936 2797 2468
Fragments arranged by size:
2797 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI