(Linear) (Six-base) MAPSORT of: KIEE3945.seq Check: 1876 from: 1 to: 1818 KIEE3945.orf With 182 enzymes: SgfI * March 22, 2012 16:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 106 1818 Size: 106 1712 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 40 361 1139 1514 1532 1709 1748 1818 Size: 40 321 778 375 18 177 39 70 Fragments arranged by size: 778 375 321 177 70 40 39 18 AflIII A'CryG_T Cuts at: 0 1786 1818 Size: 1786 32 AlwNI CAG_nnn'CTG Cuts at: 0 534 1818 Size: 534 1284 ApoI r'AATT_y Cuts at: 0 91 466 1445 1579 1675 1818 Size: 91 375 979 134 96 143 Fragments arranged by size: 979 375 143 134 96 91 AvaI C'yCGr_G Cuts at: 0 441 1818 Size: 441 1377 BamHI G'GATC_C Cuts at: 0 237 1818 Size: 237 1581 BanII G_rGCy'C Cuts at: 0 1600 1818 Size: 1600 218 BbsI GAAGACnn'nnnn_ Cuts at: 0 121 820 1818 Size: 121 699 998 Fragments arranged by size: 998 699 121 BciVI GTATCCnnnnn_n' Cuts at: 0 673 1818 Size: 673 1145 BglII A'GATC_T Cuts at: 0 72 1428 1818 Size: 72 1356 390 Fragments arranged by size: 1356 390 72 BlpI GC'TnA_GC Cuts at: 0 989 1818 Size: 989 829 BmrI ACTGGGnnnn_n' Cuts at: 0 827 1818 Size: 827 991 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 727 1684 1818 Size: 727 957 134 Fragments arranged by size: 957 727 134 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1457 1487 1818 Size: 1457 30 331 Fragments arranged by size: 1457 331 30 BseYI C'CCAG_C Cuts at: 0 8 1025 1735 1818 Size: 8 1017 710 83 Fragments arranged by size: 1017 710 83 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 965 1818 Size: 965 853 BsiHKAI G_wGCw'C Cuts at: 0 1600 1818 Size: 1600 218 BsmI GAATG_Cn' Cuts at: 0 973 1818 Size: 973 845 Bsp1286I G_dGCh'C Cuts at: 0 1600 1818 Size: 1600 218 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 106 1818 Size: 106 1712 BsrGI T'GTAC_A Cuts at: 0 486 1381 1818 Size: 486 895 437 Fragments arranged by size: 895 486 437 BstEII G'GTnAC_C Cuts at: 0 868 1818 Size: 868 950 BstXI CCAn_nnnn'nTGG Cuts at: 0 1608 1818 Size: 1608 210 BstYI r'GATC_y Cuts at: 0 72 237 341 1428 1624 1818 Size: 72 165 104 1087 196 194 Fragments arranged by size: 1087 196 194 165 104 72 BtgI C'CryG_G Cuts at: 0 956 1818 Size: 956 862 BtsI GCAGTG_nn' Cuts at: 0 602 947 1818 Size: 602 345 871 Fragments arranged by size: 871 602 345 ClaI AT'CG_AT Cuts at: 0 98 1818 Size: 98 1720 DraI TTT'AAA Cuts at: 0 1793 1818 Size: 1793 25 EaeI y'GGCC_r Cuts at: 0 326 1314 1818 Size: 326 988 504 Fragments arranged by size: 988 504 326 EarI CTCTTCn'nnn_ Cuts at: 0 497 1174 1804 1818 Size: 497 677 630 14 Fragments arranged by size: 677 630 497 14 EcoICRI GAG'CTC Cuts at: 0 1598 1818 Size: 1598 220 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 40 361 727 1139 1514 1532 1684 1709 Size: 40 321 366 412 375 18 152 25 Cuts at: 1709 1748 1818 Size: 39 70 Fragments arranged by size: 412 375 366 321 152 70 40 39 25 18 EcoNI CCTnn'n_nnAGG Cuts at: 0 55 1818 Size: 55 1763 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 149 877 1537 1818 Size: 149 728 660 281 Fragments arranged by size: 728 660 281 149 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1335 1367 1457 1489 1818 Size: 1335 32 90 32 329 Fragments arranged by size: 1335 329 90 32 32 HindIII A'AGCT_T Cuts at: 0 1538 1818 Size: 1538 280 MfeI C'AATT_G Cuts at: 0 1637 1818 Size: 1637 181 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1116 1818 Size: 1116 702 MscI TGG'CCA Cuts at: 0 328 1316 1818 Size: 328 988 502 Fragments arranged by size: 988 502 328 MslI CAynn'nnrTG Cuts at: 0 1128 1818 Size: 1128 690 MspA1I CmG'CkG Cuts at: 0 12 1818 Size: 12 1806 NdeI CA'TA_TG Cuts at: 0 1702 1818 Size: 1702 116 PfoI T'CCnGG_A Cuts at: 0 77 1818 Size: 77 1741 PshAI GACnn'nnGTC Cuts at: 0 125 1818 Size: 125 1693 SacI G_AGCT'C Cuts at: 0 1600 1818 Size: 1600 218 ScaI AGT'ACT Cuts at: 0 181 1818 Size: 181 1637 SfcI C'TryA_G Cuts at: 0 1090 1818 Size: 1090 728 SspI AAT'ATT Cuts at: 0 691 1818 Size: 691 1127 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 597 1162 1591 1698 1818 Size: 597 565 429 107 120 Fragments arranged by size: 597 565 429 120 107 TatI w'GTAC_w Cuts at: 0 179 272 486 1381 1818 Size: 179 93 214 895 437 Fragments arranged by size: 895 437 214 179 93 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1046 1078 1818 Size: 1046 32 740 Fragments arranged by size: 1046 740 32 XmnI GAAnn'nnTTC Cuts at: 0 91 1008 1818 Size: 91 917 810 Fragments arranged by size: 917 810 91 Enzymes that do cut: AarI AcuI AflIII AlwNI ApoI AvaI BamHI BanII BbsI BciVI BglII BlpI BmrI BpmI BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrGI BstEII BstXI BstYI BtgI BtsI ClaI DraI EaeI EarI EcoICRI Eco57MI EcoNI FalI Hin4I HindIII MfeI MmeI MscI MslI MspA1I NdeI PfoI PshAI SacI ScaI SfcI SspI TaqII TatI TstI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BanI BbeI BbvCI BcgI BclI BfrBI BglI Bme1580I BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI CspCI DraIII DrdI EagI EciI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII HincII HpaI KasI KpnI MluI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI StyI SwaI Tth111I XbaI XcmI XhoI XmaI ZraI