(Circular) (Six-base) MAPSORT of: pFN21AE3935.seq Check: 789 from: 1 to: 6103 pFN21AE3935 6103 bp With 182 enzymes: SgfI * November 18, 2016 17:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2716 2716 Size: 6103 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3913 278 Size: 53 83 186 936 2377 2468 Fragments arranged by size: 2468 2377 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2169 3446 1937 Size: 232 1277 4594 Fragments arranged by size: 4594 1277 232 Acc65I G'GTAC_C Cuts at: 2500 3424 2500 Size: 924 5179 AclI AA'CG_TT Cuts at: 1563 2336 4230 4603 1563 Size: 773 1894 373 3063 Fragments arranged by size: 3063 1894 773 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2380 2472 2493 4166 5550 5818 1632 Size: 748 92 21 1673 1384 268 1917 Fragments arranged by size: 1917 1673 1384 748 268 92 21 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5906 AflIII A'CryG_T Cuts at: 1203 5002 5979 1203 Size: 3799 977 1327 Fragments arranged by size: 3799 1327 977 AgeI A'CCGG_T Cuts at: 4934 4934 Size: 6103 AhdI GACnn_n'nnGTC Cuts at: 4832 4832 Size: 6103 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6103 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6103 AlwNI CAG_nnn'CTG Cuts at: 2591 2627 2708 2807 3846 5418 2591 Size: 36 81 99 1039 1572 3276 Fragments arranged by size: 3276 1572 1039 99 81 36 ApaLI G'TGCA_C Cuts at: 4160 5316 4160 Size: 1156 4947 ApoI r'AATT_y Cuts at: 1493 2045 2372 3412 3638 3790 5899 1493 Size: 552 327 1040 226 152 2109 1697 Fragments arranged by size: 2109 1697 1040 552 327 226 152 AseI AT'TA_AT Cuts at: 160 1032 4657 160 Size: 872 3625 1606 Fragments arranged by size: 3625 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6103 AvaI C'yCGr_G Cuts at: 1870 1943 3249 3306 3428 1870 Size: 73 1306 57 122 4545 Fragments arranged by size: 4545 1306 122 73 57 BamHI G'GATC_C Cuts at: 3433 3800 3433 Size: 367 5736 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2500 3424 4879 618 Size: 325 217 627 713 924 1455 1842 Fragments arranged by size: 1842 1455 924 713 627 325 217 BanII G_rGCy'C Cuts at: 1391 3422 1391 Size: 2031 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2742 3251 928 Size: 971 843 509 3780 Fragments arranged by size: 3780 971 843 509 BbvCI CC'TCA_GC Cuts at: 2895 2895 Size: 6103 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4294 4328 1903 Size: 34 2357 34 3678 Fragments arranged by size: 3678 2357 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2805 3995 5205 2805 Size: 1190 1210 3703 Fragments arranged by size: 3703 1210 1190 BclI T'GATC_A Cuts at: 1548 2527 1548 Size: 979 5124 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4714 136 Size: 107 122 71 631 605 3042 1525 Fragments arranged by size: 3042 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6098 6098 Size: 6103 BlpI GC'TnA_GC Cuts at: 3520 3520 Size: 6103 Bme1580I G_kGCm'C Cuts at: 1790 4164 5320 1790 Size: 2374 1156 2573 Fragments arranged by size: 2573 2374 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6103 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2781 2817 3834 Size: 416 53 83 399 24 1364 36 1017 Cuts at: 3834 4792 442 Size: 958 1753 Fragments arranged by size: 1753 1364 1017 958 416 399 83 53 36 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6103 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2715 3354 2715 Size: 639 5464 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2685 3318 4763 1239 Size: 1446 633 1445 2579 Fragments arranged by size: 2579 1446 1445 633 Bpu10I CC'TnA_GC Cuts at: 2649 2895 3188 2649 Size: 246 293 5564 Fragments arranged by size: 5564 293 246 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2761 4228 5093 5391 2761 Size: 1467 865 298 3473 Fragments arranged by size: 3473 1467 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4766 882 Size: 617 3267 2219 Fragments arranged by size: 3267 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3894 493 Size: 711 2690 2702 Fragments arranged by size: 2702 2690 711 BsaBI GATnn'nnATC Cuts at: 6097 6097 Size: 6103 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3910 4292 4927 275 Size: 53 83 186 936 2377 382 635 1451 Fragments arranged by size: 2377 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4536 4934 5208 5355 5766 4536 Size: 398 274 147 411 4873 Fragments arranged by size: 4873 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3339 3369 1178 Size: 30 165 30 1936 30 3912 Fragments arranged by size: 3912 1936 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2335 2459 2582 1186 Size: 1149 124 123 4707 Fragments arranged by size: 4707 1149 124 123 BseYI C'CCAG_C Cuts at: 3316 5306 5802 3316 Size: 1990 496 3617 Fragments arranged by size: 3617 1990 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3377 1163 Size: 573 1641 3889 Fragments arranged by size: 3889 1641 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3271 3553 4314 5342 1540 Size: 275 186 1270 282 761 1028 2301 Fragments arranged by size: 2301 1270 1028 761 282 275 186 BsiHKAI G_wGCw'C Cuts at: 4164 4249 5320 4164 Size: 85 1071 4947 Fragments arranged by size: 4947 1071 85 BsmI GAATG_Cn' Cuts at: 3614 3707 3614 Size: 93 6010 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3422 4164 4249 5320 1391 Size: 399 1632 742 85 1071 2174 Fragments arranged by size: 2174 1632 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5766 5766 Size: 6103 BspHI T'CATG_A Cuts at: 3049 3992 5722 3049 Size: 943 1730 3430 Fragments arranged by size: 3430 1730 943 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2716 3051 3391 3458 844 Size: 1872 335 340 67 3489 Fragments arranged by size: 3489 1872 340 335 67 BsrBI CCG'CTC Cuts at: 1394 1823 3990 1394 Size: 429 2167 3507 Fragments arranged by size: 3507 2167 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4598 4772 65 Size: 1183 3350 174 1396 Fragments arranged by size: 3350 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4747 4934 1700 Size: 110 2937 187 2869 Fragments arranged by size: 2937 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6103 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6103 BssSI C'ACGA_G Cuts at: 4163 5175 4163 Size: 1012 5091 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6103 BstBI TT'CG_AA Cuts at: 4907 4907 Size: 6103 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6103 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6103 BstYI r'GATC_y Cuts at: 1972 3433 3800 4185 4202 5654 5740 5752 6098 Size: 1461 367 385 17 1452 86 12 346 Cuts at: 6098 1972 Size: 1977 Fragments arranged by size: 1977 1461 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2352 2439 2493 2676 2352 Size: 87 54 183 5779 Fragments arranged by size: 5779 183 87 54 BtgI C'CryG_G Cuts at: 513 1065 2004 2128 3343 3395 513 Size: 552 939 124 1215 52 3221 Fragments arranged by size: 3221 1215 939 552 124 52 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5364 Fragments arranged by size: 5364 683 56 BtsI GCAGTG_nn' Cuts at: 2066 3619 4413 4433 2066 Size: 1553 794 20 3736 Fragments arranged by size: 3736 1553 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6103 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6068 DraI TTT'AAA Cuts at: 3407 3407 Size: 6103 DraIII CAC_nnn'GTG Cuts at: 2789 2789 Size: 6103 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5110 809 Size: 507 3794 1802 Fragments arranged by size: 3794 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3550 4439 6068 Size: 54 1425 50 275 18 1720 889 1629 Cuts at: 6068 8 Size: 43 Fragments arranged by size: 1720 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3550 1537 Size: 275 1738 4090 Fragments arranged by size: 4090 1738 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2122 2684 3035 3231 4033 1353 Size: 769 562 351 196 802 3423 Fragments arranged by size: 3423 802 769 562 351 196 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4690 5064 5210 387 Size: 1064 138 3101 374 146 1280 Fragments arranged by size: 3101 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2380 2472 2493 2685 3318 4166 4763 Size: 393 748 92 21 192 633 848 597 Cuts at: 4763 5550 5818 1239 Size: 787 268 1524 Fragments arranged by size: 1524 848 787 748 633 597 393 268 192 92 21 EcoO109I rG'GnC_Cy Cuts at: 2080 2948 3293 3302 3335 2080 Size: 868 345 9 33 4848 Fragments arranged by size: 4848 868 345 33 9 EcoRI G'AATT_C Cuts at: 3412 3790 3412 Size: 378 5725 EcoRV GAT'ATC Cuts at: 2392 2392 Size: 6103 FspI TGC'GCA Cuts at: 3113 4609 3113 Size: 1496 4607 HaeII r_GCGC'y Cuts at: 1678 5250 1678 Size: 3572 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2803 2835 3204 3236 4744 Size: 32 135 32 1231 32 369 32 1508 Cuts at: 4744 4776 4818 4850 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1508 1231 369 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3447 3693 669 Size: 988 281 1509 246 3079 Fragments arranged by size: 3079 1509 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6103 HpaI GTT'AAC Cuts at: 3693 3693 Size: 6103 KpnI G_GTAC'C Cuts at: 2504 3428 2504 Size: 924 5179 MfeI C'AATT_G Cuts at: 3702 3702 Size: 6103 MluI A'CGCG_T Cuts at: 5979 5979 Size: 6103 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5217 5401 1305 Size: 3912 184 2007 Fragments arranged by size: 3912 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4281 Fragments arranged by size: 4281 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2244 4061 4420 4579 518 Size: 648 843 235 1817 359 159 2042 Fragments arranged by size: 2042 1817 843 648 359 235 159 MspA1I CmG'CkG Cuts at: 1606 2899 3519 4196 5344 5589 1606 Size: 1293 620 677 1148 245 2120 Fragments arranged by size: 2120 1293 1148 677 620 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2128 513 Size: 552 939 124 4488 Fragments arranged by size: 4488 939 552 124 NdeI CA'TA_TG Cuts at: 387 387 Size: 6103 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6103 NotI GC'GGCC_GC Cuts at: 3550 3550 Size: 6103 NspI r_CATG'y Cuts at: 1132 1229 2092 3461 5006 1132 Size: 97 863 1369 1545 2229 Fragments arranged by size: 2229 1545 1369 863 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6103 PciI A'CATG_T Cuts at: 5002 5002 Size: 6103 PflMI CCAn_nnn'nTGG Cuts at: 1108 2287 2789 2807 3371 1108 Size: 1179 502 18 564 3840 Fragments arranged by size: 3840 1179 564 502 18 PmeI GTTT'AAAC Cuts at: 3407 3407 Size: 6103 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4172 4204 5685 5717 4172 Size: 32 1481 32 4558 Fragments arranged by size: 4558 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2948 3293 2948 Size: 345 5758 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6103 PsiI TTA'TAA Cuts at: 3673 3673 Size: 6103 PspXI vC'TCGA_Gb Cuts at: 3249 3306 3249 Size: 57 6046 PstI C_TGCA'G Cuts at: 830 2713 3455 830 Size: 1883 742 3478 Fragments arranged by size: 3478 1883 742 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6103 SalI G'TCGA_C Cuts at: 1936 3445 1936 Size: 1509 4594 SanDI GG'GwC_CC Cuts at: 2948 2948 Size: 6103 SbfI CC_TGCA'GG Cuts at: 3455 3455 Size: 6103 ScaI AGT'ACT Cuts at: 4351 6063 4351 Size: 1712 4391 SfcI C'TryA_G Cuts at: 826 1045 2709 3451 4586 5267 5458 826 Size: 219 1664 742 1135 681 191 1471 Fragments arranged by size: 1664 1471 1135 742 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6103 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6103 SmaI CCC'GGG Cuts at: 1872 3430 1872 Size: 1558 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2740 3249 3306 4207 5108 5370 Size: 197 926 797 509 57 901 901 262 Cuts at: 5370 820 Size: 1553 Fragments arranged by size: 1553 926 901 901 797 509 262 197 57 SnaBI TAC'GTA Cuts at: 493 3894 493 Size: 3401 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6103 SphI G_CATG'C Cuts at: 1132 3461 1132 Size: 2329 3774 SspI AAT'ATT Cuts at: 5 52 2932 4027 5 Size: 47 2880 1095 2081 Fragments arranged by size: 2880 2081 1095 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6103 StyI C'CwwG_G Cuts at: 513 1065 2004 2128 3542 513 Size: 552 939 124 1414 3074 Fragments arranged by size: 3074 1414 939 552 124 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2800 3285 3308 4130 4147 4300 4485 Size: 1255 1220 485 23 822 17 153 185 Cuts at: 4485 4930 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1220 822 485 445 185 153 23 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2728 4349 6061 Size: 274 80 33 51 1443 751 1621 1712 Cuts at: 6061 96 Size: 138 Fragments arranged by size: 1712 1621 1443 751 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3087 3119 3339 3360 3371 3392 6063 6095 3087 Size: 32 220 21 11 21 2671 32 3095 Fragments arranged by size: 3095 2671 220 32 32 21 21 11 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6103 XbaI T'CTAG_A Cuts at: 3439 3439 Size: 6103 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3032 3032 Size: 6103 XhoI C'TCGA_G Cuts at: 1943 3249 3306 1943 Size: 1306 57 4740 Fragments arranged by size: 4740 1306 57 XmaI C'CCGG_G Cuts at: 1870 3428 1870 Size: 1558 4545 XmnI GAAnn'nnTTC Cuts at: 4232 4232 Size: 6103 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3911 276 Size: 53 83 186 936 2377 2468 Fragments arranged by size: 2468 2377 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI