Restriction map for
pFN21AE0440
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspHI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BciVI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaJI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I CelII CfoI Cfr10I
Cfr13I ClaI CpoI DraII DraIII
DrdI DsaI EaeI EagI EclXI
Eco47III Eco52I EcoO109I EcoO65I EcoRI
EcoT14I FauI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HindIII HpaII KasI KpnI KspI
MaeI MamI MfeI MflI MluI
MluNI MroI MscI MslI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI Sau96I SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 490 GTAGAC GT^[AC][GT]AC
AhdI 384 GACTTCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 128 GATCC GGATC(4/5)
AocI 309 CCTTAGG CC^T[ACGT]AGG
Asp700I 74 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 384 GACTTCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI 377 GAAGAC GAAGAC(2/6)
BbvI 901 GCTGC GCAGC(8/12)
BcgI 12 GCATCAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 377 GAAGAC GAAGAC(2/6)
BseRI 100 GAGGAG GAGGAG(10/8)
BsmAI 497 GAGAC GTCTC(1/5)
Bsp1407I 520 TGTACA T^GTACA
BspMI 897 ACCTGC ACCTGC(4/8)
BsrGI 520 TGTACA T^GTACA
BssKI 37 CCAGG ^CC[ACGT]GG
BstNI 37 CCAGG CC^[AT]GG
Bsu36I 309 CCTTAGG CC^T[ACGT]AGG
Cac8I 435 GCCAGC GC[ACGT]^[ACGT]GC
DraI 480 TTTAAA TTT^AAA
Eam1105I 384 GACTTCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 309 CCTTAGG CC^T[ACGT]AGG
EcoRII 37 CCAGG ^CC[AT]GG
EcoRV 262 GATATC GAT^ATC
EcoT22I 65 ATGCAT ATGCA^T
Fnu4HI 901 GCTGC GC^[ACGT]GC
HaeIII 561 GGCC GG^CC
HincII 274 GTTAAC GT[CT]^[GA]AC
HindII 274 GTTAAC GT[CT]^[GA]AC
HpaI 274 GTTAAC GTT^AAC
HphI 895 TCACC GGTGA(8/7)
ItaI 901 GCTGC GC^[ACGT]GC
MvaI 37 CCAGG CC^[AT]GG
NlaIII 64 CATG CATG^
NsiI 65 ATGCAT ATGCA^T
NspI 63 ACATGC [GA]CATG^[CT]
ScaI 242 AGTACT AGT^ACT
ScrFI 37 CCAGG CC^[ACGT]GG
SfcI 662 CTGTAG C^T[GA][CT]AG
SspBI 520 TGTACA T^GTACA
TaqI 126 TCGA T^CGA
TseI 901 GCTGC G^C[AT]GC
TthHB8I 126 TCGA T^CGA
XmnI 74 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
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AcsI 323 AAATTC [GA]^AATT[CT]
428 AAATTT
ApoI 323 AAATTC [GA]^AATT[CT]
428 AAATTT
BclI 291 TGATCA T^GATCA
864 TGATCA
BstF5I 105 GGATG GGATG(2/0)
738 GGATG
FbaI 291 TGATCA T^GATCA
864 TGATCA
FokI 105 GGATG GGATG(9/13)
738 GGATG
MaeII 390 ACGT A^CGT
612 ACGT
MaeIII 461 GTGAC ^GT[ACGT]AC
894 GTCAC
MspA1I 467 CAGCTG C[AC]G^C[GT]G
659 CAGCTG
MwoI 562 GCCTTAACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
721 GCAATTTTAGC
PleI 463 GACTC GAGTC(4/5)
763 GACTC
PvuII 467 CAGCTG CAG^CTG
659 CAGCTG
SfaNI 106 GATGC GCATC(5/9)
12 GCATC
Tsp45I 461 GTGAC ^GT[GC]AC
894 GTCAC
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# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AfaI AluI BsiYI BslI BsrI
DdeI DpnII EarI EcoNI HinfI
Ksp632I MboI MboII MnlI MseI
NdeII RsaI Sau3AI TfiI Tru9I
Tsp509I TspRI