################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## Acc65I AccI AclI AflII AgeI AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AviII AvrII BamHI BbeI BbrPI BcgI BciVI BclI BfaI BfrI BglII BlnI BsaAI BsiWI BsmBI BsmI BspHI BspLU11I BspMI BsrDI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CpoI DraI DrdI Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HpaI HphI KasI KpnI KspI MaeI MfeI MluI MseI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I RcaI RsrII SacII SalI SapI ScaI SexAI SfoI SfuI SnaBI SpeI SphI SspI SwaI TfiI Tru9I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AccIII 338 TCCGGA T^CCGGA AflIII 701 ACGTGT A^C[GA][CT]GT AhdI 670 GACCGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 232 GTGCAC G^TGCAC AlwI 371 GGATC GGATC(4/5) ApaLI 232 GTGCAC G^TGCAC AspEI 670 GACCGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 775 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 797 CCCGGG C^[CT]CG[GA]G BalI 1220 TGGCCA TGG^CCA BbsI 361 GAAGAC GAAGAC(2/6) BlpI 39 GCTGAGC GC^T[ACGT]AGC Bpu1102I 39 GCTGAGC GC^T[ACGT]AGC BpuAI 361 GAAGAC GAAGAC(2/6) BsaBI 504 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 268 GAGACC GGTCTC(1/5) BsaWI 338 TCCGGA [AT]^CCGG[AT] BseAI 338 TCCGGA T^CCGGA BsoBI 797 CCCGGG C^[CT]CG[GA]G Bsp1407I 590 TGTACA T^GTACA BspDI 253 ATCGAT AT^CGAT BspEI 338 TCCGGA T^CCGGA BsrGI 590 TGTACA T^GTACA BstXI 272 CCATCGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 371 GGATCT [GA]^GATC[CT] CelII 39 GCTGAGC GC^T[ACGT]AGC ClaI 253 ATCGAT AT^CGAT DraIII 697 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 670 GACCGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC HaeII 1066 AGCGCC [GA]GCGC^[CT] HindIII 1018 AAGCTT A^AGCTT MaeIII 825 GTCAC ^GT[ACGT]AC MamI 504 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 371 GGATCT [GA]^GATC[CT] MluNI 1220 TGGCCA TGG^CCA MroI 338 TCCGGA T^CCGGA MscI 1220 TGGCCA TGG^CCA MslI 972 CACAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 118 CAGCTG C[AC]G^C[GT]G NcoI 977 CCATGG C^CATGG NspI 1060 ACATGC [GA]CATG^[CT] PflFI 775 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 216 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 958 CTGCAG CTGCA^G PvuI 137 CGATCG CGAT^CG PvuII 118 CAGCTG CAG^CTG SbfI 957 CCTGCAGG CCTGCA^GG SfcI 958 CTGCAG C^T[GA][CT]AG SgrAI 1176 CGCCGGCG C[GA]^CCGG[CT]G SmaI 797 CCCGGG CCC^GGG SmlI 643 CTCAAG C^T[CT][GA]AG Sse8387I 957 CCTGCAGG CCTGCA^GG SspBI 590 TGTACA T^GTACA Tsp45I 825 GTCAC ^GT[GC]AC Tth111I 775 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 216 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 371 GGATCT [GA]^GATC[CT] XmaCI 797 CCCGGG C^CCGGG XmaI 797 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AatII 160 GACGTC GACGT^C 184 GACGTC AccII 1107 CGCG CG^CG 1131 CGCG AcsI 66 GAATTT [GA]^AATT[CT] 564 GAATTT AcyI 160 GACGTC G[GA]^CG[CT]C 184 GACGTC AfaI 249 GTAC GT^AC 591 GTAC ApaI 150 GGGCCC GGGCC^C 800 GGGCCC ApoI 66 GAATTT [GA]^AATT[CT] 564 GAATTT BanI 487 GGCACC G^G[CT][GA]CC 1138 GGCACC BbvCI 1005 GCTGAGG CCTCAGC(-5/-2) 1120 GCTGAGG BpmI 223 CTGGAG CTGGAG(16/14) 433 CTGGAG BsaHI 160 GACGTC G[GA]^CG[CT]C 184 GACGTC BseRI 1233 CTCCTC GAGGAG(10/8) 142 GAGGAG BsgI 90 GTGCAG GTGCAG(16/14) 115 GTGCAG BsmAI 268 GAGAC GTCTC(1/5) 1161 GAGAC BsmFI 992 GGGAC GGGAC(10/14) 219 GTCCC BssSI 399 CACGAG CACGAG(-5/-1) 499 CACGAG BstUI 1107 CGCG CG^CG 1131 CGCG CfoI 1067 GCGC GCG^C 1108 GCGC DsaI 623 CCGTGG C^C[GA][CT]GG 977 CCATGG EagI 133 CGGCCG C^GGCCG 766 CGGCCG EarI 829 CTCTTC CTCTTC(1/4) 21 GAAGAG EclXI 133 CGGCCG C^GGCCG 766 CGGCCG Eco52I 133 CGGCCG C^GGCCG 766 CGGCCG EcoNI 588 CCTGTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 804 CCTGCGGCAGG EcoT14I 977 CCATGG C^C[AT][AT]GG 1226 CCAAGG HgaI 1105 GACGC GACGC(5/10) 1129 GACGC HhaI 1067 GCGC GCG^C 1108 GCGC Hin1I 160 GACGTC G[GA]^CG[CT]C 184 GACGTC HinP1I 1067 GCGC G^CGC 1108 GCGC Ksp632I 829 CTCTTC CTCTTC(1/4) 21 GAAGAG MvnI 1107 CGCG CG^CG 1131 CGCG PshAI 180 GACTGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 270 GACCATCGTC RsaI 249 GTAC GT^AC 591 GTAC SfaNI 256 GATGC GCATC(5/9) 836 GCATC SfiI 1212 GGCCTCCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 1221 GGCCACCAAGGCC StyI 977 CCATGG C^C[AT][AT]GG 1226 CCAAGG Tsp509I 67 AATT ^AATT 565 AATT TspRI 815 CACTG CA[GC]TG[ACGT][ACGT]^ 951 CACTG XcmI 422 CCATGCGGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 986 CCACAGGGGACGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AciI AluI AlwNI AspHI AvaII BanII BbvI BcnI BglI BmyI BsaJI BsiEI BsiHKAI BsiYI BslI Bsp1286I BsrBI BsrFI BsrI BssKI BstF5I BstNI Cac8I Cfr10I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MaeII MboI MboII MnlI MspI MvaI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PleI SacI Sau3AI Sau96I ScrFI StuI TaqI TseI TthHB8I