Restriction map for
pFN21AE3657
(Circular) (Six-base) MAPSORT of: pFN21AE3657.seq Check: 3880 from: 1 to: 5947
pFN21AE3657 5947 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2169 2193 3757 278
Size: 53 83 186 936 633 24 1564 2468
Fragments arranged by size:
2468 1564 936 633 186 83 53 24
AccI GT'mk_AC
Cuts at: 1937 3290 1937
Size: 1353 4594
Acc65I G'GTAC_C
Cuts at: 3268 3268
Size: 5947
AclI AA'CG_TT
Cuts at: 1563 4074 4447 1563
Size: 2511 373 3063
Fragments arranged by size:
3063 2511 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2039 4010 5394 5662 1632
Size: 407 1971 1384 268 1917
Fragments arranged by size:
1971 1917 1384 407 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5750
AflIII A'CryG_T
Cuts at: 1203 2706 4846 5823 1203
Size: 1503 2140 977 1327
Fragments arranged by size:
2140 1503 1327 977
AgeI A'CCGG_T
Cuts at: 4778 4778
Size: 5947
AhdI GACnn_n'nnGTC
Cuts at: 2680 4676 2680
Size: 1996 3951
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5947
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5947
AlwNI CAG_nnn'CTG
Cuts at: 2227 2379 2821 3690 5262 2227
Size: 152 442 869 1572 2912
Fragments arranged by size:
2912 1572 869 442 152
ApaI G_GGCC'C
Cuts at: 2159 2809 2159
Size: 650 5297
ApaLI G'TGCA_C
Cuts at: 2237 4004 5160 2237
Size: 1767 1156 3024
Fragments arranged by size:
3024 1767 1156
ApoI r'AATT_y
Cuts at: 1493 2071 2569 3256 3482 3634 5743 1493
Size: 578 498 687 226 152 2109 1697
Fragments arranged by size:
2109 1697 687 578 498 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4501 160
Size: 872 3469 1606
Fragments arranged by size:
3469 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
AvaI C'yCGr_G
Cuts at: 1870 1943 2802 3272 1870
Size: 73 859 470 4545
Fragments arranged by size:
4545 859 470 73
BamHI G'GATC_C
Cuts at: 3277 3644 3277
Size: 367 5580
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2492 3143 3268 4723 618
Size: 325 217 627 705 651 125 1455 1842
Fragments arranged by size:
1842 1455 705 651 627 325 217 125
BanII G_rGCy'C
Cuts at: 1391 2159 2340 2403 2799 2809 3266 1391
Size: 768 181 63 396 10 457 4072
Fragments arranged by size:
4072 768 457 396 181 63 10
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2373 928
Size: 971 474 4502
Fragments arranged by size:
4502 971 474
BbvCI CC'TCA_GC
Cuts at: 3011 3126 3011
Size: 115 5832
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4138 4172 1903
Size: 34 50 34 2117 34 3678
Fragments arranged by size:
3678 2117 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3839 5049 3839
Size: 1210 4737
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5947
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3038 3054 3224
Size: 107 122 71 631 605 1366 16 170
Cuts at: 3224 3233 4558 136
Size: 9 1325 1525
Fragments arranged by size:
1525 1366 1325 631 605 170 122 107
71 16 9
BglII A'GATC_T
Cuts at: 5942 5942
Size: 5947
BlpI GC'TnA_GC
Cuts at: 2045 3364 2045
Size: 1319 4628
Bme1580I G_kGCm'C
Cuts at: 1790 2159 2241 2809 4008 5164 1790
Size: 369 82 568 1199 1156 2573
Fragments arranged by size:
2573 1199 1156 568 369 82
BmgBI CAC'GTC
Cuts at: 1318 3001 1318
Size: 1683 4264
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2831 3678 4636
Size: 416 53 83 399 24 1414 847 958
Cuts at: 4636 442
Size: 1753
Fragments arranged by size:
1753 1414 958 847 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5947
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2249 2459 4607 1239
Size: 1010 210 2148 2579
Fragments arranged by size:
2579 2148 1010 210
Bpu10I CC'TnA_GC
Cuts at: 2132 3011 3126 2132
Size: 879 115 4953
Fragments arranged by size:
4953 879 115
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2633 4072 4937 5235 2633
Size: 1439 865 298 3345
Fragments arranged by size:
3345 1439 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2267 4610 882
Size: 617 768 2343 2219
Fragments arranged by size:
2343 2219 768 617
BsaAI yAC'GTr
Cuts at: 493 1204 3738 493
Size: 711 2534 2702
Fragments arranged by size:
2702 2534 711
BsaBI GATnn'nnATC
Cuts at: 2513 5941 2513
Size: 3428 2519
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2166 2190 3754 4136
Size: 53 83 186 936 633 24 1564 382
Cuts at: 4136 4771 275
Size: 635 1451
Fragments arranged by size:
1564 1451 936 635 633 382 186 83
53 24
BsaWI w'CCGG_w
Cuts at: 2343 4380 4778 5052 5199 5610 2343
Size: 2037 398 274 147 411 2680
Fragments arranged by size:
2680 2037 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5722
Fragments arranged by size:
5722 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2162 3229 1186
Size: 976 1067 3904
Fragments arranged by size:
3904 1067 976
BseYI C'CCAG_C
Cuts at: 3194 5150 5646 3194
Size: 1956 496 3495
Fragments arranged by size:
3495 1956 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2116 2141 1163
Size: 573 380 25 4969
Fragments arranged by size:
4969 573 380 25
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2141 2145 2774 3397 4158 5186
Size: 275 186 140 4 629 623 761 1028
Cuts at: 5186 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 629 623 275 186 140
4
BsiHKAI G_wGCw'C
Cuts at: 2241 2340 2403 2799 2978 4008 4093 5164 2241
Size: 99 63 396 179 1030 85 1071 3024
Fragments arranged by size:
3024 1071 1030 396 179 99 85 63
BsmI GAATG_Cn'
Cuts at: 3458 3551 3458
Size: 93 5854
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2159 2241 2340 2403 2799 2809 2978
Size: 399 369 82 99 63 396 10 169
Cuts at: 2978 3266 4008 4093 5164 1391
Size: 288 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 396 369 288 169
99 85 82 63 10
BspEI T'CCGG_A
Cuts at: 2343 5610 2343
Size: 3267 2680
BspHI T'CATG_A
Cuts at: 3836 5566 3836
Size: 1730 4217
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3302 844
Size: 2458 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2293 2322 2770 3061 3834 1394
Size: 429 470 29 448 291 773 3507
Fragments arranged by size:
3507 773 470 448 429 291 29
BsrDI GCAATG_nn'
Cuts at: 65 1248 4442 4616 65
Size: 1183 3194 174 1396
Fragments arranged by size:
3194 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2136 3037 3182 4591 4778 1700
Size: 110 326 901 145 1409 187 2869
Fragments arranged by size:
2869 1409 901 326 187 145 110
BsrGI T'GTAC_A
Cuts at: 96 2595 96
Size: 2499 3448
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5947
BssSI C'ACGA_G
Cuts at: 2404 2504 4007 5019 2404
Size: 100 1503 1012 3332
Fragments arranged by size:
3332 1503 1012 100
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5947
BstBI TT'CG_AA
Cuts at: 4751 4751
Size: 5947
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5947
BstXI CCAn_nnnn'nTGG
Cuts at: 2284 2284
Size: 5947
BstYI r'GATC_y
Cuts at: 1972 2376 3277 3644 4029 4046 5498 5584 5596
Size: 404 901 367 385 17 1452 86 12
Cuts at: 5596 5942 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 901 404 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2628 2982 513
Size: 552 1563 354 3478
Fragments arranged by size:
3478 1563 552 354
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2935 487
Size: 683 56 1709 3499
Fragments arranged by size:
3499 1709 683 56
BtsI GCAGTG_nn'
Cuts at: 3463 4257 4277 3463
Size: 794 20 5133
Fragments arranged by size:
5133 794 20
ClaI AT'CG_AT
Cuts at: 1554 2259 1554
Size: 705 5242
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5912
DraI TTT'AAA
Cuts at: 3251 3251
Size: 5947
DraIII CAC_nnn'GTG
Cuts at: 2707 2707
Size: 5947
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4954 809
Size: 507 3638 1802
Fragments arranged by size:
3638 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2138 2771 3030
Size: 54 1425 50 275 18 308 633 259
Cuts at: 3030 3225 3394 4283 5912 8
Size: 195 169 889 1629 43
Fragments arranged by size:
1629 1425 889 633 308 275 259 195
169 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2138 2771 3394 1537
Size: 275 326 633 623 4090
Fragments arranged by size:
4090 633 623 326 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2021 2840 3877 1353
Size: 668 819 1037 3423
Fragments arranged by size:
3423 1037 819 668
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4534 4908 5054 387
Size: 1064 138 2945 374 146 1280
Fragments arranged by size:
2945 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2338 2401 2797 2338
Size: 63 396 5488
Fragments arranged by size:
5488 396 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2249 2459 4010 4607 5394 5662
Size: 393 407 210 210 1551 597 787 268
Cuts at: 5662 1239
Size: 1524
Fragments arranged by size:
1551 1524 787 597 407 393 268 210
210
EcoNI CCTnn'n_nnAGG
Cuts at: 2597 2813 2597
Size: 216 5731
EcoO109I rG'GnC_Cy
Cuts at: 2155 2156 2533 2640 2806 2155
Size: 1 377 107 166 5296
Fragments arranged by size:
5296 377 166 107 1
EcoRI G'AATT_C
Cuts at: 3256 3634 3256
Size: 378 5569
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2467 2467
Size: 5947
FspI TGC'GCA
Cuts at: 4453 4453
Size: 5947
HaeII r_GCGC'y
Cuts at: 1678 3075 5094 1678
Size: 1397 2019 2531
Fragments arranged by size:
2531 2019 1397
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2207 2239 2666 2698 2711
Size: 32 135 32 635 32 427 32 13
Cuts at: 2711 2743 4588 4620 4662 4694 1373
Size: 32 1845 32 42 32 2626
Fragments arranged by size:
2626 1845 635 427 135 42 32 32
32 32 32 32 32 13
HincII GTy'rAC
Cuts at: 669 1657 1938 3291 3537 669
Size: 988 281 1353 246 3079
Fragments arranged by size:
3079 1353 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3023 748
Size: 2275 3672
HpaI GTT'AAC
Cuts at: 3537 3537
Size: 5947
KpnI G_GTAC'C
Cuts at: 3272 3272
Size: 5947
MfeI C'AATT_G
Cuts at: 3546 3546
Size: 5947
MluI A'CGCG_T
Cuts at: 5823 5823
Size: 5947
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5061 5245 1305
Size: 3756 184 2007
Fragments arranged by size:
3756 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3227 10
Size: 54 1425 343 1395 2730
Fragments arranged by size:
2730 1425 1395 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2981 3905 4264 4423 518
Size: 648 1815 924 359 159 2042
Fragments arranged by size:
2042 1815 924 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2125 3363 4040 5188 5433 1606
Size: 519 1238 677 1148 245 2120
Fragments arranged by size:
2120 1238 1148 677 519 245
NaeI GCC'GGC
Cuts at: 2138 3039 3184 2138
Size: 901 145 4901
Fragments arranged by size:
4901 901 145
NcoI C'CATG_G
Cuts at: 513 1065 2982 513
Size: 552 1917 3478
Fragments arranged by size:
3478 1917 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5947
NgoMIV G'CCGG_C
Cuts at: 2136 3037 3182 2136
Size: 901 145 4901
Fragments arranged by size:
4901 901 145
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5947
NotI GC'GGCC_GC
Cuts at: 3394 3394
Size: 5947
NspI r_CATG'y
Cuts at: 1132 1229 3069 3305 4850 1132
Size: 97 1840 236 1545 2229
Fragments arranged by size:
2229 1840 1545 236 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5947
PciI A'CATG_T
Cuts at: 4846 4846
Size: 5947
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2227 1108
Size: 1119 4828
PfoI T'CCnGG_A
Cuts at: 2247 2283 2790 2247
Size: 36 507 5404
Fragments arranged by size:
5404 507 36
PmeI GTTT'AAAC
Cuts at: 3251 3251
Size: 5947
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2747 2779 4016 4048 5529 5561 2747
Size: 32 1237 32 1481 32 3133
Fragments arranged by size:
3133 1481 1237 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2189 2279 1613
Size: 576 90 5281
Fragments arranged by size:
5281 576 90
PsiI TTA'TAA
Cuts at: 3517 3517
Size: 5947
PspOMI G'GGCC_C
Cuts at: 2155 2805 2155
Size: 650 5297
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2588 2620 2588
Size: 32 5915
PstI C_TGCA'G
Cuts at: 830 2967 3299 830
Size: 2137 332 3478
Fragments arranged by size:
3478 2137 332
PvuI CG_AT'CG
Cuts at: 2001 2145 2001
Size: 144 5803
PvuII CAG'CTG
Cuts at: 2125 2125
Size: 5947
SacI G_AGCT'C
Cuts at: 2340 2403 2799 2340
Size: 63 396 5488
Fragments arranged by size:
5488 396 63
SalI G'TCGA_C
Cuts at: 1936 3289 1936
Size: 1353 4594
SbfI CC_TGCA'GG
Cuts at: 2967 3299 2967
Size: 332 5615
ScaI AGT'ACT
Cuts at: 4195 5907 4195
Size: 1712 4235
SfcI C'TryA_G
Cuts at: 826 1045 2963 3295 4430 5111 5302 826
Size: 219 1918 332 1135 681 191 1471
Fragments arranged by size:
1918 1471 1135 681 332 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3224 3233 3224
Size: 9 5938
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
SgrAI Cr'CCGG_yG
Cuts at: 1700 3182 1700
Size: 1482 4465
SmaI CCC'GGG
Cuts at: 1872 2804 3274 1872
Size: 932 470 4545
Fragments arranged by size:
4545 932 470
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2648 4051 4952 5214 820
Size: 197 926 705 1403 901 262 1553
Fragments arranged by size:
1553 1403 926 901 705 262 197
SnaBI TAC'GTA
Cuts at: 493 3738 493
Size: 3245 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5947
SphI G_CATG'C
Cuts at: 1132 3305 1132
Size: 2173 3774
SspI AAT'ATT
Cuts at: 5 52 3871 5
Size: 47 3819 2081
Fragments arranged by size:
3819 2081 47
StuI AGG'CCT
Cuts at: 1517 2081 2357 3236 1517
Size: 564 276 879 4228
Fragments arranged by size:
4228 879 564 276
StyI C'CwwG_G
Cuts at: 513 1065 2982 3231 3386 513
Size: 552 1917 249 155 3074
Fragments arranged by size:
3074 1917 552 249 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2719 3974 3991 4144 4329 4774 325
Size: 1255 1139 1255 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1255 1139 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2253 2595 4193
Size: 274 80 33 51 1443 276 342 1598
Cuts at: 4193 5905 96
Size: 1712 138
Fragments arranged by size:
1712 1598 1443 342 276 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5907 5939 5907
Size: 32 5915
Tth111I GACn'n_nGTC
Cuts at: 1316 2783 1316
Size: 1467 4480
XbaI T'CTAG_A
Cuts at: 3283 3283
Size: 5947
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2434 2998 2434
Size: 564 5383
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5947
XmaI C'CCGG_G
Cuts at: 1870 2802 3272 1870
Size: 932 470 4545
Fragments arranged by size:
4545 932 470
XmnI GAAnn'nnTTC
Cuts at: 4076 4076
Size: 5947
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2167 2191 3755 276
Size: 53 83 186 936 633 24 1564 2468
Fragments arranged by size:
2468 1564 936 633 186 83 53 24
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PmlI PpuMI PspXI
RsrII SacII SanDI SapI SexAI SfoI SrfI SwaI