(Circular) (Six-base) MAPSORT of: pFN21AE0437.seq Check: 7426 from: 1 to: 5635 pFN21AE0437 5635 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3445 278 Size: 53 83 186 936 1909 2468 Fragments arranged by size: 2468 1909 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2010 2978 1937 Size: 73 968 4594 Fragments arranged by size: 4594 968 73 Acc65I G'GTAC_C Cuts at: 2956 2956 Size: 5635 AclI AA'CG_TT Cuts at: 1563 3762 4135 1563 Size: 2199 373 3063 Fragments arranged by size: 3063 2199 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2415 3698 5082 5350 1632 Size: 783 1283 1384 268 1917 Fragments arranged by size: 1917 1384 1283 783 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5438 AflIII A'CryG_T Cuts at: 1203 4534 5511 1203 Size: 3331 977 1327 Fragments arranged by size: 3331 1327 977 AgeI A'CCGG_T Cuts at: 4466 4466 Size: 5635 AhdI GACnn_n'nnGTC Cuts at: 2893 4364 2893 Size: 1471 4164 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5635 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2445 1184 Size: 1261 4374 AlwNI CAG_nnn'CTG Cuts at: 2065 2162 3378 4950 2065 Size: 97 1216 1572 2750 Fragments arranged by size: 2750 1572 1216 97 ApaLI G'TGCA_C Cuts at: 3692 4848 3692 Size: 1156 4479 ApoI r'AATT_y Cuts at: 1493 2944 3170 3322 5431 1493 Size: 1451 226 152 2109 1697 Fragments arranged by size: 2109 1697 1451 226 152 AseI AT'TA_AT Cuts at: 160 1032 4189 160 Size: 872 3157 1606 Fragments arranged by size: 3157 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5635 AvaI C'yCGr_G Cuts at: 1870 1943 2383 2960 1870 Size: 73 440 577 4545 Fragments arranged by size: 4545 577 440 73 BamHI G'GATC_C Cuts at: 2965 3332 2965 Size: 367 5268 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2956 4411 618 Size: 325 217 627 1169 1455 1842 Fragments arranged by size: 1842 1455 1169 627 325 217 BanII G_rGCy'C Cuts at: 1391 2572 2703 2954 1391 Size: 1181 131 251 4072 Fragments arranged by size: 4072 1181 251 131 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2875 928 Size: 971 976 3688 Fragments arranged by size: 3688 976 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3826 3860 1903 Size: 34 1889 34 3678 Fragments arranged by size: 3678 1889 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3527 4737 3527 Size: 1210 4425 BclI T'GATC_A Cuts at: 1548 2739 1548 Size: 1191 4444 BfrBI ATG'CAT Cuts at: 2762 2762 Size: 5635 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4246 136 Size: 107 122 71 631 605 2574 1525 Fragments arranged by size: 2574 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5630 5630 Size: 5635 BlpI GC'TnA_GC Cuts at: 2433 2640 3052 2433 Size: 207 412 5016 Fragments arranged by size: 5016 412 207 Bme1580I G_kGCm'C Cuts at: 1790 2390 3696 4852 1790 Size: 600 1306 1156 2573 Fragments arranged by size: 2573 1306 1156 600 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5635 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2198 3366 4324 Size: 416 53 83 399 24 781 1168 958 Cuts at: 4324 442 Size: 1753 Fragments arranged by size: 1753 1168 958 781 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5635 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2623 2623 Size: 5635 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2448 2586 4295 1239 Size: 1209 138 1709 2579 Fragments arranged by size: 2579 1709 1209 138 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2442 3760 4625 4923 2442 Size: 1318 865 298 3154 Fragments arranged by size: 3154 1318 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2708 4298 882 Size: 617 1209 1590 2219 Fragments arranged by size: 2219 1590 1209 617 BsaAI yAC'GTr Cuts at: 493 1204 3426 493 Size: 711 2222 2702 Fragments arranged by size: 2702 2222 711 BsaBI GATnn'nnATC Cuts at: 5629 5629 Size: 5635 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3442 3824 4459 275 Size: 53 83 186 936 1909 382 635 1451 Fragments arranged by size: 1909 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4068 4466 4740 4887 5298 4068 Size: 398 274 147 411 4405 Fragments arranged by size: 4405 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2062 2092 2122 2150 2152 Size: 30 165 30 659 30 30 28 2 Cuts at: 2152 2180 2522 2552 2554 2584 2915 2945 1178 Size: 28 342 30 2 30 331 30 3868 Fragments arranged by size: 3868 659 342 331 165 30 30 30 30 30 30 30 28 28 2 2 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2282 2612 1186 Size: 1096 330 4209 Fragments arranged by size: 4209 1096 330 BseYI C'CCAG_C Cuts at: 4838 5334 4838 Size: 496 5139 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2562 1163 Size: 573 826 4236 Fragments arranged by size: 4236 826 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3085 3846 4874 1540 Size: 275 186 1084 761 1028 2301 Fragments arranged by size: 2301 1084 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2202 2572 2703 3696 3781 4852 2202 Size: 370 131 993 85 1071 2985 Fragments arranged by size: 2985 1071 993 370 131 85 BsmI GAATG_Cn' Cuts at: 2854 3146 3239 2854 Size: 292 93 5250 Fragments arranged by size: 5250 292 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2202 2390 2572 2703 2954 3696 3781 Size: 399 412 188 182 131 251 742 85 Cuts at: 3781 4852 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 412 399 251 188 182 131 85 BspEI T'CCGG_A Cuts at: 5298 5298 Size: 5635 BspHI T'CATG_A Cuts at: 3524 5254 3524 Size: 1730 3905 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2990 844 Size: 2146 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3522 1394 Size: 429 1699 3507 Fragments arranged by size: 3507 1699 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2802 4130 4304 65 Size: 1183 1554 1328 174 1396 Fragments arranged by size: 1554 1396 1328 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4279 4466 1700 Size: 110 2469 187 2869 Fragments arranged by size: 2869 2469 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5635 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5635 BssSI C'ACGA_G Cuts at: 3695 4707 3695 Size: 1012 4623 BstAPI GCAn_nnn'nTGC Cuts at: 794 2162 794 Size: 1368 4267 BstBI TT'CG_AA Cuts at: 2288 4439 2288 Size: 2151 3484 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5635 BstXI CCAn_nnnn'nTGG Cuts at: 2564 2564 Size: 5635 BstYI r'GATC_y Cuts at: 1972 2965 3332 3717 3734 5186 5272 5284 5630 Size: 993 367 385 17 1452 86 12 346 Cuts at: 5630 1972 Size: 1977 Fragments arranged by size: 1977 1452 993 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2502 2502 Size: 5635 BtgI C'CryG_G Cuts at: 513 1065 2004 2808 513 Size: 552 939 804 3340 Fragments arranged by size: 3340 939 804 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2764 487 Size: 683 56 1538 3358 Fragments arranged by size: 3358 1538 683 56 BtsI GCAGTG_nn' Cuts at: 2160 3151 3945 3965 2160 Size: 991 794 20 3830 Fragments arranged by size: 3830 991 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5635 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5600 DraI TTT'AAA Cuts at: 2939 2939 Size: 5635 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2599 4642 809 Size: 507 1283 2043 1802 Fragments arranged by size: 2043 1802 1283 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3082 3971 5600 Size: 54 1425 50 275 18 1252 889 1629 Cuts at: 5600 8 Size: 43 Fragments arranged by size: 1629 1425 1252 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3082 1537 Size: 275 1270 4090 Fragments arranged by size: 4090 1270 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2027 2100 2259 3565 1353 Size: 674 73 159 1306 3423 Fragments arranged by size: 3423 1306 674 159 73 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4222 4596 4742 387 Size: 1064 138 2633 374 146 1280 Fragments arranged by size: 2633 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2570 2701 2570 Size: 131 5504 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2415 2448 2586 3698 4295 5082 5350 Size: 393 783 33 138 1112 597 787 268 Cuts at: 5350 1239 Size: 1524 Fragments arranged by size: 1524 1112 787 783 597 393 268 138 33 EcoNI CCTnn'n_nnAGG Cuts at: 2397 2731 2397 Size: 334 5301 EcoO109I rG'GnC_Cy Cuts at: 2895 2895 Size: 5635 EcoRI G'AATT_C Cuts at: 2944 3322 2944 Size: 378 5257 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2038 2038 Size: 5635 FspI TGC'GCA Cuts at: 4141 4141 Size: 5635 HaeII r_GCGC'y Cuts at: 1678 4782 1678 Size: 3104 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2122 2150 2154 2182 2879 Size: 32 135 32 550 28 4 28 697 Cuts at: 2879 2911 4276 4308 4350 4382 1373 Size: 32 1365 32 42 32 2626 Fragments arranged by size: 2626 1365 697 550 135 42 32 32 32 32 32 28 28 4 HincII GTy'rAC Cuts at: 669 1657 1938 2337 2979 3225 669 Size: 988 281 399 642 246 3079 Fragments arranged by size: 3079 988 642 399 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5635 HpaI GTT'AAC Cuts at: 3225 3225 Size: 5635 KpnI G_GTAC'C Cuts at: 2960 2960 Size: 5635 MfeI C'AATT_G Cuts at: 3234 3234 Size: 5635 MluI A'CGCG_T Cuts at: 5511 5511 Size: 5635 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2123 2199 2597 4749 4933 1305 Size: 818 76 398 2152 184 2007 Fragments arranged by size: 2152 2007 818 398 184 76 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3813 Fragments arranged by size: 3813 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2708 2792 2807 3593 3952 4111 518 Size: 648 1542 84 15 786 359 159 2042 Fragments arranged by size: 2042 1542 786 648 359 159 84 15 MspA1I CmG'CkG Cuts at: 1606 2429 2639 3051 3728 4876 5121 1606 Size: 823 210 412 677 1148 245 2120 Fragments arranged by size: 2120 1148 823 677 412 245 210 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4144 Fragments arranged by size: 4144 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5635 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5635 NotI GC'GGCC_GC Cuts at: 3082 3082 Size: 5635 NsiI A_TGCA'T Cuts at: 2764 2764 Size: 5635 NspI r_CATG'y Cuts at: 1132 1229 2633 2762 2993 4538 1132 Size: 97 1404 129 231 1545 2229 Fragments arranged by size: 2229 1545 1404 231 129 97 PasI CC'CwG_GG Cuts at: 1348 2733 1348 Size: 1385 4250 PciI A'CATG_T Cuts at: 4534 4534 Size: 5635 PflMI CCAn_nnn'nTGG Cuts at: 1108 2127 1108 Size: 1019 4616 PmeI GTTT'AAAC Cuts at: 2939 2939 Size: 5635 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3704 3736 5217 5249 3704 Size: 32 1481 32 4090 Fragments arranged by size: 4090 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2895 2895 Size: 5635 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5635 PsiI TTA'TAA Cuts at: 3205 3205 Size: 5635 PstI C_TGCA'G Cuts at: 830 2987 830 Size: 2157 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5635 PvuII CAG'CTG Cuts at: 2639 2639 Size: 5635 SacI G_AGCT'C Cuts at: 2572 2703 2572 Size: 131 5504 SalI G'TCGA_C Cuts at: 1936 2977 1936 Size: 1041 4594 SbfI CC_TGCA'GG Cuts at: 2987 2987 Size: 5635 ScaI AGT'ACT Cuts at: 3883 5595 3883 Size: 1712 3923 SfcI C'TryA_G Cuts at: 826 1045 2983 4118 4799 4990 826 Size: 219 1938 1135 681 191 1471 Fragments arranged by size: 1938 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5635 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5635 SmaI CCC'GGG Cuts at: 1872 2962 1872 Size: 1090 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2457 3739 4640 4902 820 Size: 197 926 514 1282 901 262 1553 Fragments arranged by size: 1553 1282 926 901 514 262 197 SnaBI TAC'GTA Cuts at: 493 3426 493 Size: 2933 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5635 SphI G_CATG'C Cuts at: 1132 2993 1132 Size: 1861 3774 SspI AAT'ATT Cuts at: 5 52 3559 5 Size: 47 3507 2081 Fragments arranged by size: 3507 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5635 StyI C'CwwG_G Cuts at: 513 1065 2004 3074 513 Size: 552 939 1070 3074 Fragments arranged by size: 3074 1070 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2570 2635 3662 3679 3832 4017 4462 Size: 1255 990 65 1027 17 153 185 445 Cuts at: 4462 325 Size: 1498 Fragments arranged by size: 1498 1255 1027 990 445 185 153 65 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3881 5593 96 Size: 274 80 33 51 1443 1904 1712 138 Fragments arranged by size: 1904 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2554 2586 2713 2745 5595 5627 2554 Size: 32 127 32 2850 32 2562 Fragments arranged by size: 2850 2562 127 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5635 XbaI T'CTAG_A Cuts at: 2971 2971 Size: 5635 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2289 2289 Size: 5635 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5635 XmaI C'CCGG_G Cuts at: 1870 2960 1870 Size: 1090 4545 XmnI GAAnn'nnTTC Cuts at: 3764 3764 Size: 5635 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3443 276 Size: 53 83 186 936 1909 2468 Fragments arranged by size: 2468 1909 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI