################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## AccI AccIII AclI AflII AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AviII AvrII BbeI BbsI BciVI BclI BfrI BlnI BlpI Bpu1102I BpuAI BsaI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspEI BspMI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HindIII HpaI KasI KspI MfeI MluI MroI MunI NarI NcoI NheI NotI NruI NsiI NspV PacI PaeR7I PmeI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TfiI XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 337 GACGTC GACGT^C AflIII 369 ACATGT A^C[GA][CT]GT AlwNI 1111 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 484 CCTGAGG CC^T[ACGT]AGG Asp700I 268 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 781 GAGCTC G[AT]GC[AT]^C BalI 225 TGGCCA TGG^CCA BamHI 480 GGATCC G^GATCC BanII 781 GAGCTC G[GA]GC[CT]^C BbrPI 736 CACGTG CAC^GTG BbvCI 931 GCTGAGG CCTCAGC(-5/-2) BcgI 975 CGAGATCGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 797 GCCGGATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 41 AGATCT A^GATCT BsaBI 1024 GATAATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 358 GAGGAG GAGGAG(10/8) BsiHKAI 781 GAGCTC G[AT]GC[AT]^C BsmAI 1113 GAGAC GTCTC(1/5) BspDI 979 ATCGAT AT^CGAT BspLU11I 369 ACATGT A^CATGT BsrDI 59 GCAATG GCAATG(2/0) BstAPI 581 GCATTGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 470 CCATCTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 484 CCTGAGG CC^T[ACGT]AGG ClaI 979 ATCGAT AT^CGAT DraIII 938 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 10 CCACGG C^C[GA][CT]GG EaeI 225 TGGCCA [CT]^GGCC[GA] Eco81I 484 CCTGAGG CC^T[ACGT]AGG HincII 538 GTCAAC GT[CT]^[GA]AC HindII 538 GTCAAC GT[CT]^[GA]AC MamI 1024 GATAATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 225 TGGCCA TGG^CCA MscI 225 TGGCCA TGG^CCA MseI 425 TTAA T^TAA NaeI 764 GCCGGC GCC^GGC NdeI 1280 CATATG CA^TATG NgoMIV 764 GCCGGC G^CCGGC NspI 369 ACATGT [GA]CATG^[CT] PflMI 1110 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 736 CACGTG CAC^GTG PmlI 736 CACGTG CAC^GTG PvuII 1225 CAGCTG CAG^CTG SacI 781 GAGCTC GAGCT^C SexAI 1103 ACCTGGT A^CC[AT]GGT StuI 1342 AGGCCT AGG^CCT Tru9I 425 TTAA T^TAA Van91I 1110 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 268 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### Acc65I 1070 GGTACC G^GTACC 1076 GGTACC AccII 91 CGCG CG^CG 544 CGCG AcsI 268 GAATTT [GA]^AATT[CT] 1016 AAATTC AgeI 1067 ACCGGT A^CCGGT 1073 ACCGGT ApoI 268 GAATTT [GA]^AATT[CT] 1016 AAATTC Asp718I 1070 GGTACC G^GTACC 1076 GGTACC BcnI 237 CCGGG CC^[GC]GG 1335 CCGGG BfaI 613 CTAG C^TAG 853 CTAG BsaAI 673 TACGTG [CT]AC^GT[GA] 736 CACGTG BsaWI 1067 ACCGGT [AT]^CCGG[AT] 1073 ACCGGT BsgI 76 CTGCAC GTGCAG(16/14) 1249 CTGCAC BspHI 572 TCATGA T^CATGA 725 TCATGA BsrBI 71 CCGCTC CCGCTC(-3/-3) 747 CCGCTC BstUI 91 CGCG CG^CG 544 CGCG EarI 289 GAAGAG CTCTTC(1/4) 912 GAAGAG EcoNI 351 CCTTAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1206 CCTCGACAAGG HgaI 4 GCGTC GACGC(5/10) 989 GCGTC HphI 678 GGTGA GGTGA(8/7) 1401 TCACC KpnI 1070 GGTACC GGTAC^C 1076 GGTACC Ksp632I 289 GAAGAG CTCTTC(1/4) 912 GAAGAG MaeI 613 CTAG C^TAG 853 CTAG MspA1I 1225 CAGCTG C[AC]G^C[GT]G 1246 CCGCTG MvnI 91 CGCG CG^CG 544 CGCG NciI 237 CCGGG CC^[GC]GG 1335 CCGGG PinAI 1067 ACCGGT A^CCGGT 1073 ACCGGT RcaI 572 TCATGA T^CATGA 725 TCATGA ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AspI AvaI AvaII BanI BbvI BmyI BpmI BsaHI BsaJI BsiYI BslI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII ItaI MaeII MaeIII MboI MboII MflI MnlI MslI MspI MvaI MwoI NdeII NlaIII NlaIV PflFI PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI TaqI TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XhoII