################################## # NO CUT ENZYMES ( 142 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspI AvaI AviII AvrII BamHI BbeI BbvCI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KspI MamI MluI MroI MslI MspA1I MvnI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### Acc65I 1146 GGTACC G^GTACC Asp718I 1146 GGTACC G^GTACC AspHI 787 GTGCTC G[AT]GC[AT]^C AvaII 1239 GGACC G^G[AT]CC BalI 486 TGGCCA TGG^CCA BanII 854 GGGCTC G[GA]GC[CT]^C BbrPI 868 CACGTG CAC^GTG BbvI 120 GCAGC GCAGC(8/12) BcnI 15 CCCGG CC^[GC]GG BsaAI 868 CACGTG [CT]AC^GT[GA] BsiHKAI 787 GTGCTC G[AT]GC[AT]^C BsmFI 831 GTCCC GGGAC(10/14) BsmI 1004 GCATTC GAATGC(1/-1) BsrDI 230 CATTGC GCAATG(2/0) BstAPI 213 GCACAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 388 GGTCACC G^GT[ACGT]ACC BstPI 388 GGTCACC G^GT[ACGT]ACC DraII 1238 AGGACCC [GA]G^G[ACGT]CC[CT] DsaI 529 CCATGG C^C[GA][CT]GG EaeI 486 TGGCCA [CT]^GGCC[GA] EcoO109I 1238 AGGACCC [GA]G^G[ACGT]CC[CT] EcoO65I 388 GGTCACC G^GT[ACGT]ACC FauI 589 CCCGC CCCGC(4/6) HapII 16 CCGG C^CGG HincII 240 GTCAAC GT[CT]^[GA]AC HindII 240 GTCAAC GT[CT]^[GA]AC HindIII 689 AAGCTT A^AGCTT HpaII 16 CCGG C^CGG KpnI 1146 GGTACC GGTAC^C MaeII 869 ACGT A^CGT MfeI 520 CAATTG C^AATTG MluNI 486 TGGCCA TGG^CCA MscI 486 TGGCCA TGG^CCA MspI 16 CCGG C^CGG MunI 520 CAATTG C^AATTG NciI 15 CCCGG CC^[GC]GG NcoI 529 CCATGG C^CATGG NheI 802 GCTAGC G^CTAGC PmaCI 868 CACGTG CAC^GTG PmlI 868 CACGTG CAC^GTG PpuMI 1238 AGGACCC [GA]G^G[AT]CC[CT] SapI 437 GAAGAGC GCTCTTC(1/4) SmlI 1217 CTCAAG C^T[CT][GA]AG SpeI 508 ACTAGT A^CTAGT SspI 1123 AATATT AAT^ATT TaqI 1335 TCGA T^CGA TseI 120 GCAGC G^C[AT]GC TthHB8I 1335 TCGA T^CGA XcmI 290 CCACAGCAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### Asp700I 58 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1033 GAAATAATTC BanI 18 GGTGCC G^G[CT][GA]CC 1146 GGTACC BglII 34 AGATCT A^GATCT 1137 AGATCT BpmI 315 CTCCAG CTGGAG(16/14) 1253 CTGGAG BsaI 533 GGTCTC GGTCTC(1/5) 756 GGTCTC BsaJI 134 CCAAGG C^C[ACGT][ACGT]GG 529 CCATGG BsiYI 9 CCTCAACCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 846 CCCTCATGGGG BslI 9 CCTCAACCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 846 CCCTCATGGGG BspMI 361 GCAGGT ACCTGC(4/8) 442 GCAGGT BstYI 34 AGATCT [GA]^GATC[CT] 1137 AGATCT Cfr13I 607 GGGCC G^G[ACGT]CC 1239 GGACC DpnII 35 GATC ^GATC 1138 GATC DraI 105 TTTAAA TTT^AAA 1245 TTTAAA EcoT14I 134 CCAAGG C^C[AT][AT]GG 529 CCATGG Fnu4HI 120 GCAGC GC^[ACGT]GC 210 GCCGC ItaI 120 GCAGC GC^[ACGT]GC 210 GCCGC MboI 35 GATC ^GATC 1138 GATC MflI 34 AGATCT [GA]^GATC[CT] 1137 AGATCT NdeII 35 GATC ^GATC 1138 GATC PleI 781 GAGTC GAGTC(4/5) 829 GAGTC Sau3AI 35 GATC ^GATC 1138 GATC Sau96I 607 GGGCC G^G[ACGT]CC 1239 GGACC StyI 134 CCAAGG C^C[AT][AT]GG 529 CCATGG XhoII 34 AGATCT [GA]^GATC[CT] 1137 AGATCT XmnI 58 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1033 GAAATAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI BbsI BfaI BmyI BpuAI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I DdeI EarI EcoRII FokI HaeIII HinfI HphI Ksp632I MaeI MaeIII MboII MnlI MseI MvaI MwoI NlaIII NlaIV RsaI ScrFI SfaNI SfcI TfiI Tru9I Tsp45I Tsp509I TspRI