Restriction map for
pFN21AE3616
(Circular) (Six-base) MAPSORT of: pFN21AE3616.seq Check: 7286 from: 1 to: 6139
pFN21AE3616 6139 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2357 2357
Size: 6139
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3949 278
Size: 53 83 186 936 2413 2468
Fragments arranged by size:
2468 2413 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3482 1937
Size: 1545 4594
Acc65I G'GTAC_C
Cuts at: 3151 3460 3151
Size: 309 5830
AclI AA'CG_TT
Cuts at: 1563 4266 4639 1563
Size: 2703 373 3063
Fragments arranged by size:
3063 2703 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2106 2158 2192 2441 2484 2712 3167 3177
Size: 474 52 34 249 43 228 455 10
Cuts at: 3177 4202 5586 5854 1632
Size: 1025 1384 268 1917
Fragments arranged by size:
1917 1384 1025 474 455 268 249 228
52 43 34 10
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5942
AflIII A'CryG_T
Cuts at: 1203 5038 6015 1203
Size: 3835 977 1327
Fragments arranged by size:
3835 1327 977
AgeI A'CCGG_T
Cuts at: 4970 4970
Size: 6139
AhdI GACnn_n'nnGTC
Cuts at: 4868 4868
Size: 6139
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6139
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6139
AlwNI CAG_nnn'CTG
Cuts at: 3882 5454 3882
Size: 1572 4567
ApaLI G'TGCA_C
Cuts at: 4196 5352 4196
Size: 1156 4983
ApoI r'AATT_y
Cuts at: 1493 2773 3116 3253 3359 3448 3674 3826 5935
Size: 1280 343 137 106 89 226 152 2109
Cuts at: 5935 1493
Size: 1697
Fragments arranged by size:
2109 1697 1280 343 226 152 137 106
89
AseI AT'TA_AT
Cuts at: 160 1032 4693 160
Size: 872 3661 1606
Fragments arranged by size:
3661 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6139
AvaI C'yCGr_G
Cuts at: 1870 1943 3464 1870
Size: 73 1521 4545
Fragments arranged by size:
4545 1521 73
BamHI G'GATC_C
Cuts at: 3469 3836 3469
Size: 367 5772
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2023 3151 3460 4915 618
Size: 325 217 627 236 1128 309 1455 1842
Fragments arranged by size:
1842 1455 1128 627 325 309 236 217
BanII G_rGCy'C
Cuts at: 1391 2863 3458 1391
Size: 1472 595 4072
Fragments arranged by size:
4072 1472 595
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2172 2726 3222 928
Size: 971 273 554 496 3845
Fragments arranged by size:
3845 971 554 496 273
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4330 4364 1903
Size: 34 2393 34 3678
Fragments arranged by size:
3678 2393 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4031 5241 4031
Size: 1210 4929
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6139
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4750 136
Size: 107 122 71 631 605 3078 1525
Fragments arranged by size:
3078 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2039 3142 6134 2039
Size: 1103 2992 2044
Fragments arranged by size:
2992 2044 1103
BlpI GC'TnA_GC
Cuts at: 3556 3556
Size: 6139
Bme1580I G_kGCm'C
Cuts at: 1790 2312 4200 5356 1790
Size: 522 1888 1156 2573
Fragments arranged by size:
2573 1888 1156 522
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6139
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2315 3870 4828
Size: 416 53 83 399 24 898 1555 958
Cuts at: 4828 442
Size: 1753
Fragments arranged by size:
1753 1555 958 898 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2811 1056
Size: 1755 4384
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2857 2857
Size: 6139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2305 3279 4799 1239
Size: 1066 974 1520 2579
Fragments arranged by size:
2579 1520 1066 974
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3207 4264 5129 5427 3207
Size: 1057 865 298 3919
Fragments arranged by size:
3919 1057 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2544 2767 4802 882
Size: 617 1045 223 2035 2219
Fragments arranged by size:
2219 2035 1045 617 223
BsaAI yAC'GTr
Cuts at: 493 1204 2875 3930 493
Size: 711 1671 1055 2702
Fragments arranged by size:
2702 1671 1055 711
BsaBI GATnn'nnATC
Cuts at: 6133 6133
Size: 6139
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3946 4328 4963 275
Size: 53 83 186 936 2413 382 635 1451
Fragments arranged by size:
2413 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4572 4970 5244 5391 5802 4572
Size: 398 274 147 411 4909
Fragments arranged by size:
4909 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3252 3282 1178
Size: 30 165 30 1849 30 4035
Fragments arranged by size:
4035 1849 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6139
BseYI C'CCAG_C
Cuts at: 2957 5342 5838 2957
Size: 2385 496 3258
Fragments arranged by size:
3258 2385 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5566
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3589 4350 5378 1540
Size: 275 186 1588 761 1028 2301
Fragments arranged by size:
2301 1588 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2796 4200 4285 5356 2796
Size: 1404 85 1071 3579
Fragments arranged by size:
3579 1404 1071 85
BsmI GAATG_Cn'
Cuts at: 3009 3650 3743 3009
Size: 641 93 5405
Fragments arranged by size:
5405 641 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2312 2796 2863 3458 4200 4285 5356
Size: 399 522 484 67 595 742 85 1071
Cuts at: 5356 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 595 522 484 399 85
67
BspEI T'CCGG_A
Cuts at: 5802 5802
Size: 6139
BspHI T'CATG_A
Cuts at: 4028 5758 4028
Size: 1730 4409
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2357 2438 3494 844
Size: 1513 81 1056 3489
Fragments arranged by size:
3489 1513 1056 81
BsrBI CCG'CTC
Cuts at: 1394 1823 4026 1394
Size: 429 2203 3507
Fragments arranged by size:
3507 2203 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2234 4634 4808 65
Size: 1183 986 2400 174 1396
Fragments arranged by size:
2400 1396 1183 986 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4783 4970 1700
Size: 110 2973 187 2869
Fragments arranged by size:
2973 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6139
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6139
BssSI C'ACGA_G
Cuts at: 4199 5211 4199
Size: 1012 5127
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2224 794
Size: 1430 4709
BstBI TT'CG_AA
Cuts at: 4943 4943
Size: 6139
BstEII G'GTnAC_C
Cuts at: 1184 2393 1184
Size: 1209 4930
BstYI r'GATC_y
Cuts at: 1972 2039 3142 3469 3836 4221 4238 5690 5776
Size: 67 1103 327 367 385 17 1452 86
Cuts at: 5776 5788 6134 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1103 385 367 346 327 86
67 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2534 513
Size: 552 939 530 4118
Fragments arranged by size:
4118 939 552 530
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5400
Fragments arranged by size:
5400 683 56
BtsI GCAGTG_nn'
Cuts at: 3655 4449 4469 3655
Size: 794 20 5325
Fragments arranged by size:
5325 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6139
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6104
DraI TTT'AAA
Cuts at: 2112 3252 3443 2112
Size: 1140 191 4808
Fragments arranged by size:
4808 1140 191
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5146 809
Size: 507 3830 1802
Fragments arranged by size:
3830 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2491 3586 4475
Size: 54 1425 50 275 18 661 1095 889
Cuts at: 4475 6104 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1095 889 661 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3586 1537
Size: 275 1774 4090
Fragments arranged by size:
4090 1774 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2211 2286 2437 2547 3234 4069 1353
Size: 858 75 151 110 687 835 3423
Fragments arranged by size:
3423 858 835 687 151 110 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4726 5100 5246 387
Size: 1064 138 3137 374 146 1280
Fragments arranged by size:
3137 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2106 2158 2192 2305 2441 2484 2712
Size: 393 474 52 34 113 136 43 228
Cuts at: 2712 3167 3177 3279 4202 4799 5586 5854 1239
Size: 455 10 102 923 597 787 268 1524
Fragments arranged by size:
1524 923 787 597 474 455 393 268
228 136 113 102 52 43 34 10
EcoO109I rG'GnC_Cy
Cuts at: 3244 3244
Size: 6139
EcoRI G'AATT_C
Cuts at: 3448 3826 3448
Size: 378 5761
FspI TGC'GCA
Cuts at: 4645 4645
Size: 6139
HaeII r_GCGC'y
Cuts at: 1678 5286 1678
Size: 3608 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4780 4812 4854 4886 1373
Size: 32 135 32 3208 32 42 32 2626
Fragments arranged by size:
3208 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2247 3483 3729 669
Size: 988 281 309 1236 246 3079
Fragments arranged by size:
3079 1236 988 309 281 246
HindIII A'AGCT_T
Cuts at: 748 2694 748
Size: 1946 4193
HpaI GTT'AAC
Cuts at: 3729 3729
Size: 6139
KpnI G_GTAC'C
Cuts at: 3155 3464 3155
Size: 309 5830
MfeI C'AATT_G
Cuts at: 2525 3738 2525
Size: 1213 4926
MluI A'CGCG_T
Cuts at: 6015 6015
Size: 6139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5253 5437 1305
Size: 3948 184 2007
Fragments arranged by size:
3948 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2493 10
Size: 54 1425 343 661 3656
Fragments arranged by size:
3656 1425 661 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4097 4456 4615 518
Size: 648 2931 359 159 2042
Fragments arranged by size:
2931 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3555 4232 5380 5625 1606
Size: 1949 677 1148 245 2120
Fragments arranged by size:
2120 1949 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2534 513
Size: 552 939 530 4118
Fragments arranged by size:
4118 939 552 530
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6139
NheI G'CTAG_C
Cuts at: 1052 2807 1052
Size: 1755 4384
NotI GC'GGCC_GC
Cuts at: 3586 3586
Size: 6139
NspI r_CATG'y
Cuts at: 1132 1229 3497 5042 1132
Size: 97 2268 1545 2229
Fragments arranged by size:
2268 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6139
PciI A'CATG_T
Cuts at: 5038 5038
Size: 6139
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6139
PfoI T'CCnGG_A
Cuts at: 2665 2665
Size: 6139
PmeI GTTT'AAAC
Cuts at: 3443 3443
Size: 6139
PmlI CAC'GTG
Cuts at: 2875 2875
Size: 6139
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4208 4240 5721 5753 4208
Size: 32 1481 32 4594
Fragments arranged by size:
4594 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3244 3244
Size: 6139
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6139
PsiI TTA'TAA
Cuts at: 2403 3709 2403
Size: 1306 4833
PstI C_TGCA'G
Cuts at: 830 3491 830
Size: 2661 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6139
SalI G'TCGA_C
Cuts at: 1936 3481 1936
Size: 1545 4594
SapI GCTCTTCn'nnn_
Cuts at: 2437 2437
Size: 6139
SbfI CC_TGCA'GG
Cuts at: 3491 3491
Size: 6139
ScaI AGT'ACT
Cuts at: 4387 6099 4387
Size: 1712 4427
SfcI C'TryA_G
Cuts at: 826 1045 2035 2378 2585 3487 4622 5303 5494
Size: 219 990 343 207 902 1135 681 191
Cuts at: 5494 826
Size: 1471
Fragments arranged by size:
1471 1135 990 902 681 343 219 207
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6139
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6139
SmaI CCC'GGG
Cuts at: 1872 3466 1872
Size: 1594 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3222 4243 5144 5406 820
Size: 197 926 1279 1021 901 262 1553
Fragments arranged by size:
1553 1279 1021 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3930 493
Size: 3437 2702
SpeI A'CTAG_T
Cuts at: 152 2513 152
Size: 2361 3778
SphI G_CATG'C
Cuts at: 1132 3497 1132
Size: 2365 3774
SspI AAT'ATT
Cuts at: 5 52 3130 4063 5
Size: 47 3078 933 2081
Fragments arranged by size:
3078 2081 933 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6139
StyI C'CwwG_G
Cuts at: 513 1065 2004 2139 2534 3578 513
Size: 552 939 135 395 1044 3074
Fragments arranged by size:
3074 1044 939 552 395 135
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4166 4183 4336 4521 4966 325
Size: 1255 2586 17 153 185 445 1498
Fragments arranged by size:
2586 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3092 4385 6097
Size: 274 80 33 51 1443 1115 1293 1712
Cuts at: 6097 96
Size: 138
Fragments arranged by size:
1712 1443 1293 1115 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6099 6131 6099
Size: 32 6107
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6139
XbaI T'CTAG_A
Cuts at: 3475 3475
Size: 6139
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2302 2302
Size: 6139
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6139
XmaI C'CCGG_G
Cuts at: 1870 3464 1870
Size: 1594 4545
XmnI GAAnn'nnTTC
Cuts at: 2067 3042 4268 2067
Size: 975 1226 3938
Fragments arranged by size:
3938 1226 975
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3947 276
Size: 53 83 186 936 2413 2468
Fragments arranged by size:
2468 2413 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI