Restriction map for
pFN21AB6192
##################################
# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AvaII AviII AvrII BalI BbeI
BbrPI BbsI BcgI BciVI BclI
BfaI BfrI BglI BlnI BpuAI
BsaAI BsaBI BsaHI BsaI BseAI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstUI BstXI Bsu36I Cfr10I
ClaI CpoI DraI DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
Hin1I HindIII HpaI KasI KpnI
KspI MaeI MaeII MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AccI 133 GTATAC GT^[AC][GT]AC
AciI 1575 CCGC CCGC(-3/-1)
AlwNI 714 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
BamHI 305 GGATCC G^GATCC
BbvCI 915 GCTGAGG CCTCAGC(-5/-2)
BcnI 54 CCCGG CC^[GC]GG
BglII 1999 AGATCT A^GATCT
BsaWI 1360 ACCGGA [AT]^CCGG[AT]
Bst1107I 133 GTATAC GTA^TAC
BstEII 111 GGTAACC G^GT[ACGT]ACC
BstPI 111 GGTAACC G^GT[ACGT]ACC
BstZ17I 133 GTATAC GTA^TAC
Cac8I 36 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 58 GCGC GCG^C
DraIII 190 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 111 GGTAACC G^GT[ACGT]ACC
EcoT14I 834 CCTTGG C^C[AT][AT]GG
HgaI 529 GACGC GACGC(5/10)
HhaI 58 GCGC GCG^C
HinP1I 58 GCGC G^CGC
HincII 637 GTTGAC GT[CT]^[GA]AC
HindII 637 GTTGAC GT[CT]^[GA]AC
MslI 788 CATTTACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 54 CCCGG CC^[GC]GG
PflMI 799 CCAACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SexAI 1922 ACCTGGT A^CC[AT]GGT
SfcI 284 CTGTAG C^T[GA][CT]AG
SmlI 211 CTTGAG C^T[CT][GA]AG
SspI 950 AATATT AAT^ATT
StyI 834 CCTTGG C^C[AT][AT]GG
Van91I 799 CCAACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 350 ACATGT A^C[GA][CT]GT
427 ACATGT
ApaI 51 GGGCCC GGGCC^C
260 GGGCCC
BanI 179 GGCACC G^G[CT][GA]CC
2015 GGCACC
BsaJI 603 CCTGGG C^C[ACGT][ACGT]GG
834 CCTTGG
BsmAI 446 GAGAC GTCTC(1/5)
1397 GAGAC
BsmFI 527 GGGAC GGGAC(10/14)
91 GTCCC
BspLU11I 350 ACATGT A^CATGT
427 ACATGT
BsrDI 963 CATTGC GCAATG(2/0)
1718 GCAATG
DraII 259 GGGGCCC [GA]G^G[ACGT]CC[CT]
1957 AGGCCCT
EcoO109I 259 GGGGCCC [GA]G^G[ACGT]CC[CT]
1957 AGGCCCT
EcoRI 1016 GAATTC G^AATTC
1604 GAATTC
HapII 55 CCGG C^CGG
1361 CCGG
HinfI 1590 GATTC G^A[ACGT]TC
2109 GATTC
HpaII 55 CCGG C^CGG
1361 CCGG
HphI 253 GGTGA GGTGA(8/7)
471 TCACC
MseI 1319 TTAA T^TAA
1760 TTAA
MspA1I 86 CAGCTG C[AC]G^C[GT]G
609 CAGCTG
MspI 55 CCGG C^CGG
1361 CCGG
PvuII 86 CAGCTG CAG^CTG
609 CAGCTG
TaqI 707 TCGA T^CGA
1425 TCGA
TfiI 1590 GATTC G^A[AT]TC
2109 GATTC
Tru9I 1319 TTAA T^TAA
1760 TTAA
TthHB8I 707 TCGA T^CGA
1425 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I AspHI BanII BbvI BlpI
BmyI BpmI Bpu1102I BseRI BsgI
BsiHKAI BsiYI BslI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
CelII Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI FokI HaeIII ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MvaI MwoI NdeII NlaIII
NlaIV NspI RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI StuI
TseI Tsp45I Tsp509I TspRI XhoII
XmnI