(Circular) (Six-base) MAPSORT of: pFN21AB6192.seq Check: 4139 from: 1 to: 6823 pFN21AB6192 6823 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4633 278 Size: 53 83 186 936 3097 2468 Fragments arranged by size: 3097 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2139 4166 1937 Size: 202 2027 4594 Fragments arranged by size: 4594 2027 202 Acc65I G'GTAC_C Cuts at: 4144 4144 Size: 6823 AclI AA'CG_TT Cuts at: 1563 4950 5323 1563 Size: 3387 373 3063 Fragments arranged by size: 3387 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2762 2802 2967 3055 3139 3223 3391 3475 Size: 1130 40 165 88 84 84 168 84 Cuts at: 3475 3559 4063 4886 6270 6538 1632 Size: 84 504 823 1384 268 1917 Fragments arranged by size: 1917 1384 1130 823 504 268 168 165 88 84 84 84 84 40 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6626 AflIII A'CryG_T Cuts at: 1203 2355 2432 5722 6699 1203 Size: 1152 77 3290 977 1327 Fragments arranged by size: 3290 1327 1152 977 77 AgeI A'CCGG_T Cuts at: 5654 5654 Size: 6823 AhdI GACnn_n'nnGTC Cuts at: 5552 5552 Size: 6823 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6823 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6823 AlwNI CAG_nnn'CTG Cuts at: 2724 4566 6138 2724 Size: 1842 1572 3409 Fragments arranged by size: 3409 1842 1572 ApaI G_GGCC'C Cuts at: 2060 2269 2060 Size: 209 6614 ApaLI G'TGCA_C Cuts at: 4880 6036 4880 Size: 1156 5667 ApoI r'AATT_y Cuts at: 1493 2815 2852 2936 3021 3609 3737 3821 4132 Size: 1322 37 84 85 588 128 84 311 Cuts at: 4132 4358 4510 6619 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1322 588 311 226 152 128 85 84 84 37 AseI AT'TA_AT Cuts at: 160 1032 5377 160 Size: 872 4345 1606 Fragments arranged by size: 4345 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6823 AvaI C'yCGr_G Cuts at: 1870 1943 4148 1870 Size: 73 2205 4545 Fragments arranged by size: 4545 2205 73 BamHI G'GATC_C Cuts at: 2310 4153 4520 2310 Size: 1843 367 4613 Fragments arranged by size: 4613 1843 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2184 4020 4144 5599 618 Size: 325 217 627 397 1836 124 1455 1842 Fragments arranged by size: 1842 1836 1455 627 397 325 217 124 BanII G_rGCy'C Cuts at: 1391 2060 2166 2269 2552 2750 4142 1391 Size: 669 106 103 283 198 1392 4072 Fragments arranged by size: 4072 1392 669 283 198 106 103 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5852 BbvCI CC'TCA_GC Cuts at: 2921 2921 Size: 6823 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5014 5048 1903 Size: 34 3077 34 3678 Fragments arranged by size: 3678 3077 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4715 5925 4715 Size: 1210 5613 BclI T'GATC_A Cuts at: 1548 1548 Size: 6823 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5434 136 Size: 107 122 71 631 605 3762 1525 Fragments arranged by size: 3762 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4004 6818 4004 Size: 2814 4009 BlpI GC'TnA_GC Cuts at: 2089 2394 3345 4240 2089 Size: 305 951 895 4672 Fragments arranged by size: 4672 951 895 305 Bme1580I G_kGCm'C Cuts at: 1790 2060 2269 4884 6040 1790 Size: 270 209 2615 1156 2573 Fragments arranged by size: 2615 2573 1156 270 209 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6823 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2871 3543 4554 Size: 416 53 83 399 24 1454 672 1011 Cuts at: 4554 5512 442 Size: 958 1753 Fragments arranged by size: 1753 1454 1011 958 672 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6823 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2769 2769 Size: 6823 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2612 3135 3289 3303 3471 3571 3625 3639 Size: 1373 523 154 14 168 100 54 14 Cuts at: 3639 3709 3807 3877 3891 5483 1239 Size: 70 98 70 14 1592 2579 Fragments arranged by size: 2579 1592 1373 523 168 154 100 98 70 70 54 14 14 14 Bpu10I CC'TnA_GC Cuts at: 2921 2921 Size: 6823 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2237 4948 5813 6111 2237 Size: 2711 865 298 2949 Fragments arranged by size: 2949 2711 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5486 882 Size: 617 3987 2219 Fragments arranged by size: 3987 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4614 493 Size: 711 3410 2702 Fragments arranged by size: 3410 2702 711 BsaBI GATnn'nnATC Cuts at: 6817 6817 Size: 6823 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4630 5012 5647 275 Size: 53 83 186 936 3097 382 635 1451 Fragments arranged by size: 3097 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3365 5256 5654 5928 6075 6486 3365 Size: 1891 398 274 147 411 3702 Fragments arranged by size: 3702 1891 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3317 3347 1178 Size: 30 165 30 1914 30 4654 Fragments arranged by size: 4654 1914 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2168 2432 2615 2840 1186 Size: 982 264 183 225 5169 Fragments arranged by size: 5169 982 264 225 183 BseYI C'CCAG_C Cuts at: 2015 6026 6522 2015 Size: 4011 496 2316 Fragments arranged by size: 4011 2316 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2737 3238 3406 3574 1163 Size: 573 1001 501 168 168 4412 Fragments arranged by size: 4412 1001 573 501 168 168 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4273 5034 6062 1540 Size: 275 186 2272 761 1028 2301 Fragments arranged by size: 2301 2272 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2166 2496 2552 2750 4884 4969 6040 2166 Size: 330 56 198 2134 85 1071 2949 Fragments arranged by size: 2949 2134 1071 330 198 85 56 BsmI GAATG_Cn' Cuts at: 2470 3305 3473 3893 4334 4427 2470 Size: 835 168 420 441 93 4866 Fragments arranged by size: 4866 835 441 420 168 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2060 2166 2269 2496 2552 2750 4142 Size: 399 270 106 103 227 56 198 1392 Cuts at: 4142 4884 4969 6040 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1392 1071 742 399 270 227 198 106 103 85 56 BspEI T'CCGG_A Cuts at: 6486 6486 Size: 6823 BspHI T'CATG_A Cuts at: 4712 6442 4712 Size: 1730 5093 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4178 844 Size: 3334 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4710 1394 Size: 429 2887 3507 Fragments arranged by size: 3507 2887 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2967 3730 5318 5492 65 Size: 1183 1719 763 1588 174 1396 Fragments arranged by size: 1719 1588 1396 1183 763 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5467 5654 1700 Size: 110 3657 187 2869 Fragments arranged by size: 3657 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6823 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6823 BssSI C'ACGA_G Cuts at: 4883 5895 4883 Size: 1012 5811 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6823 BstBI TT'CG_AA Cuts at: 5627 5627 Size: 6823 BstEII G'GTnAC_C Cuts at: 1184 2116 1184 Size: 932 5891 BstYI r'GATC_y Cuts at: 1972 2310 2323 4004 4153 4520 4905 4922 6374 Size: 338 13 1681 149 367 385 17 1452 Cuts at: 6374 6460 6472 6818 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1681 1452 385 367 346 338 149 86 17 13 12 BstZ17I GTA'TAC Cuts at: 2140 2140 Size: 6823 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5332 Fragments arranged by size: 5332 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6084 Fragments arranged by size: 6084 683 56 BtsI GCAGTG_nn' Cuts at: 3310 3394 3814 3898 4339 5133 5153 3310 Size: 84 420 84 441 794 20 4980 Fragments arranged by size: 4980 794 441 420 84 84 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6823 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2138 2173 3661 3696 3712 3747 529 Size: 35 1574 35 1488 35 16 35 3605 Fragments arranged by size: 3605 1574 1488 35 35 35 35 16 DraI TTT'AAA Cuts at: 4127 4127 Size: 6823 DraIII CAC_nnn'GTG Cuts at: 2200 2200 Size: 6823 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5830 809 Size: 507 4514 1802 Fragments arranged by size: 4514 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4270 5159 6788 Size: 54 1425 50 275 18 2440 889 1629 Cuts at: 6788 8 Size: 43 Fragments arranged by size: 2440 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4270 1537 Size: 275 2458 4090 Fragments arranged by size: 4090 2458 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2102 2540 2714 2738 2778 3142 3310 3394 Size: 749 438 174 24 40 364 168 84 Cuts at: 3394 4082 4753 1353 Size: 688 671 3423 Fragments arranged by size: 3423 749 688 671 438 364 174 168 84 40 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5410 5784 5930 387 Size: 1064 138 3821 374 146 1280 Fragments arranged by size: 3821 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2164 2550 2748 2164 Size: 386 198 6239 Fragments arranged by size: 6239 386 198 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2612 2762 2802 2967 3055 3135 3139 Size: 393 980 150 40 165 88 80 4 Cuts at: 3139 3223 3289 3303 3391 3471 3475 3559 3571 Size: 84 66 14 88 80 4 84 12 Cuts at: 3571 3625 3639 3709 3807 3877 3891 4063 4886 Size: 54 14 70 98 70 14 172 823 Cuts at: 4886 5483 6270 6538 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 980 823 787 597 393 268 172 165 150 98 88 88 84 84 80 80 70 70 66 54 40 14 14 14 12 4 4 EcoO109I rG'GnC_Cy Cuts at: 2265 3963 2265 Size: 1698 5125 EcoRI G'AATT_C Cuts at: 3021 3609 4132 4510 3021 Size: 588 523 378 5334 Fragments arranged by size: 5334 588 523 378 FspI TGC'GCA Cuts at: 5329 5329 Size: 6823 HaeII r_GCGC'y Cuts at: 1678 5970 1678 Size: 4292 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2816 2848 3072 3104 5464 Size: 32 135 32 1244 32 224 32 2360 Cuts at: 5464 5496 5538 5570 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2360 1244 224 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2644 4167 4413 669 Size: 988 281 706 1523 246 3079 Fragments arranged by size: 3079 1523 988 706 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6823 HpaI GTT'AAC Cuts at: 4413 4413 Size: 6823 KpnI G_GTAC'C Cuts at: 4148 4148 Size: 6823 MfeI C'AATT_G Cuts at: 4422 4422 Size: 6823 MluI A'CGCG_T Cuts at: 6699 6699 Size: 6823 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3360 5937 6121 1305 Size: 2055 2577 184 2007 Fragments arranged by size: 2577 2055 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5001 Fragments arranged by size: 5001 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2797 4781 5140 5299 518 Size: 648 1631 1984 359 159 2042 Fragments arranged by size: 2042 1984 1631 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2093 2616 4239 4916 6064 6309 1606 Size: 487 523 1623 677 1148 245 2120 Fragments arranged by size: 2120 1623 1148 677 523 487 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5332 Fragments arranged by size: 5332 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6823 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6823 NotI GC'GGCC_GC Cuts at: 4270 4270 Size: 6823 NspI r_CATG'y Cuts at: 1132 1229 2359 2436 2445 2802 2976 4181 5726 Size: 97 1130 77 9 357 174 1205 1545 Cuts at: 5726 1132 Size: 2229 Fragments arranged by size: 2229 1545 1205 1130 357 174 97 77 9 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6823 PciI A'CATG_T Cuts at: 2355 2432 5722 2355 Size: 77 3290 3456 Fragments arranged by size: 3456 3290 77 PflMI CCAn_nnn'nTGG Cuts at: 1108 2810 1108 Size: 1702 5121 PmeI GTTT'AAAC Cuts at: 4127 4127 Size: 6823 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4892 4924 6405 6437 4892 Size: 32 1481 32 5278 Fragments arranged by size: 5278 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6823 PsiI TTA'TAA Cuts at: 3801 4393 3801 Size: 592 6231 PspOMI G'GGCC_C Cuts at: 2056 2265 2056 Size: 209 6614 PstI C_TGCA'G Cuts at: 830 4175 830 Size: 3345 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6823 PvuII CAG'CTG Cuts at: 2093 2616 2093 Size: 523 6300 SacI G_AGCT'C Cuts at: 2166 2552 2750 2166 Size: 386 198 6239 Fragments arranged by size: 6239 386 198 SalI G'TCGA_C Cuts at: 1936 4165 1936 Size: 2229 4594 SapI GCTCTTCn'nnn_ Cuts at: 2102 2540 2738 3142 3310 2102 Size: 438 198 404 168 5615 Fragments arranged by size: 5615 438 404 198 168 SbfI CC_TGCA'GG Cuts at: 4175 4175 Size: 6823 ScaI AGT'ACT Cuts at: 5071 6783 5071 Size: 1712 5111 SexAI A'CCwGG_T Cuts at: 3927 3927 Size: 6823 SfcI C'TryA_G Cuts at: 826 1045 2289 4171 5306 5987 6178 826 Size: 219 1244 1882 1135 681 191 1471 Fragments arranged by size: 1882 1471 1244 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6823 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6823 SmaI CCC'GGG Cuts at: 1872 4150 1872 Size: 2278 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2216 4927 5828 6090 820 Size: 197 926 273 2711 901 262 1553 Fragments arranged by size: 2711 1553 926 901 273 262 197 SnaBI TAC'GTA Cuts at: 493 4614 493 Size: 4121 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6823 SphI G_CATG'C Cuts at: 1132 4181 1132 Size: 3049 3774 SspI AAT'ATT Cuts at: 5 52 2957 4747 5 Size: 47 2905 1790 2081 Fragments arranged by size: 2905 2081 1790 47 StuI AGG'CCT Cuts at: 1517 2900 3124 3544 3712 3880 1517 Size: 1383 224 420 168 168 4460 Fragments arranged by size: 4460 1383 420 224 168 168 StyI C'CwwG_G Cuts at: 513 1065 2004 2839 4262 513 Size: 552 939 835 1423 3074 Fragments arranged by size: 3074 1423 939 835 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4850 4867 5020 5205 5650 325 Size: 1255 3270 17 153 185 445 1498 Fragments arranged by size: 3270 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3526 5069 6781 Size: 274 80 33 51 1443 1549 1543 1712 Cuts at: 6781 96 Size: 138 Fragments arranged by size: 1712 1549 1543 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6783 6815 6783 Size: 32 6791 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6823 XbaI T'CTAG_A Cuts at: 4159 4159 Size: 6823 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6823 XmaI C'CCGG_G Cuts at: 1870 4148 1870 Size: 2278 4545 XmnI GAAnn'nnTTC Cuts at: 2576 2708 2984 3068 3572 4076 4952 2576 Size: 132 276 84 504 504 876 4447 Fragments arranged by size: 4447 876 504 504 276 132 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4631 276 Size: 53 83 186 936 3097 2468 Fragments arranged by size: 3097 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI