(Linear) (Six-base) MAPSORT of: KIBB6192.seq Check: 8205 from: 1 to: 2118 KIBB6192.orf With 182 enzymes: SgfI * December 27, 2011 16:29 .. AccI GT'mk_AC Cuts at: 0 134 2118 Size: 134 1984 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 757 797 962 1050 1134 1218 1386 1470 Size: 757 40 165 88 84 84 168 84 Cuts at: 1470 1554 2058 2118 Size: 84 504 60 Fragments arranged by size: 757 504 168 165 88 84 84 84 84 60 40 AflIII A'CryG_T Cuts at: 0 350 427 2118 Size: 350 77 1691 Fragments arranged by size: 1691 350 77 AlwNI CAG_nnn'CTG Cuts at: 0 719 2118 Size: 719 1399 ApaI G_GGCC'C Cuts at: 0 55 264 2118 Size: 55 209 1854 Fragments arranged by size: 1854 209 55 ApoI r'AATT_y Cuts at: 0 810 847 931 1016 1604 1732 1816 2118 Size: 810 37 84 85 588 128 84 302 Fragments arranged by size: 810 588 302 128 85 84 84 37 BamHI G'GATC_C Cuts at: 0 305 2118 Size: 305 1813 BanI G'GyrC_C Cuts at: 0 179 2015 2118 Size: 179 1836 103 Fragments arranged by size: 1836 179 103 BanII G_rGCy'C Cuts at: 0 55 161 264 547 745 2118 Size: 55 106 103 283 198 1373 Fragments arranged by size: 1373 283 198 106 103 55 BbvCI CC'TCA_GC Cuts at: 0 916 2118 Size: 916 1202 BglII A'GATC_T Cuts at: 0 1999 2118 Size: 1999 119 BlpI GC'TnA_GC Cuts at: 0 84 389 1340 2118 Size: 84 305 951 778 Fragments arranged by size: 951 778 305 84 Bme1580I G_kGCm'C Cuts at: 0 55 264 2118 Size: 55 209 1854 Fragments arranged by size: 1854 209 55 BmrI ACTGGGnnnn_n' Cuts at: 0 866 1538 2118 Size: 866 672 580 Fragments arranged by size: 866 672 580 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 764 2118 Size: 764 1354 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 607 1130 1284 1298 1466 1566 1620 1634 Size: 607 523 154 14 168 100 54 14 Cuts at: 1634 1704 1802 1872 1886 2118 Size: 70 98 70 14 232 Fragments arranged by size: 607 523 232 168 154 100 98 70 70 54 14 14 14 Bpu10I CC'TnA_GC Cuts at: 0 916 2118 Size: 916 1202 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 232 2118 Size: 232 1886 BsaWI w'CCGG_w Cuts at: 0 1360 2118 Size: 1360 758 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1312 1342 2118 Size: 1312 30 776 Fragments arranged by size: 1312 776 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 163 427 610 835 2118 Size: 163 264 183 225 1283 Fragments arranged by size: 1283 264 225 183 163 BseYI C'CCAG_C Cuts at: 0 10 2118 Size: 10 2108 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 732 1233 1401 1569 2118 Size: 732 501 168 168 549 Fragments arranged by size: 732 549 501 168 168 BsiHKAI G_wGCw'C Cuts at: 0 161 491 547 745 2118 Size: 161 330 56 198 1373 Fragments arranged by size: 1373 330 198 161 56 BsmI GAATG_Cn' Cuts at: 0 465 1300 1468 1888 2118 Size: 465 835 168 420 230 Fragments arranged by size: 835 465 420 230 168 Bsp1286I G_dGCh'C Cuts at: 0 55 161 264 491 547 745 2118 Size: 55 106 103 227 56 198 1373 Fragments arranged by size: 1373 227 198 106 103 56 55 BsrDI GCAATG_nn' Cuts at: 0 962 1725 2118 Size: 962 763 393 Fragments arranged by size: 962 763 393 BstEII G'GTnAC_C Cuts at: 0 111 2118 Size: 111 2007 BstYI r'GATC_y Cuts at: 0 305 318 1999 2118 Size: 305 13 1681 119 Fragments arranged by size: 1681 305 119 13 BstZ17I GTA'TAC Cuts at: 0 135 2118 Size: 135 1983 BtsI GCAGTG_nn' Cuts at: 0 1305 1389 1809 1893 2118 Size: 1305 84 420 84 225 Fragments arranged by size: 1305 420 225 84 84 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 133 168 1656 1691 1707 1742 2118 Size: 133 35 1488 35 16 35 376 Fragments arranged by size: 1488 376 133 35 35 35 16 DraIII CAC_nnn'GTG Cuts at: 0 195 2118 Size: 195 1923 EarI CTCTTCn'nnn_ Cuts at: 0 97 535 709 733 773 1137 1305 1389 Size: 97 438 174 24 40 364 168 84 Cuts at: 1389 2077 2118 Size: 688 41 Fragments arranged by size: 688 438 364 174 168 97 84 41 40 24 EcoICRI GAG'CTC Cuts at: 0 159 545 743 2118 Size: 159 386 198 1375 Fragments arranged by size: 1375 386 198 159 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 607 757 797 962 1050 1130 1134 1218 Size: 607 150 40 165 88 80 4 84 Cuts at: 1218 1284 1298 1386 1466 1470 1554 1566 1620 Size: 66 14 88 80 4 84 12 54 Cuts at: 1620 1634 1704 1802 1872 1886 2058 2118 Size: 14 70 98 70 14 172 60 Fragments arranged by size: 607 172 165 150 98 88 88 84 84 80 80 70 70 66 60 54 40 14 14 14 12 4 4 EcoO109I rG'GnC_Cy Cuts at: 0 260 1958 2118 Size: 260 1698 160 Fragments arranged by size: 1698 260 160 EcoRI G'AATT_C Cuts at: 0 1016 1604 2118 Size: 1016 588 514 Fragments arranged by size: 1016 588 514 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 811 843 1067 1099 2118 Size: 811 32 224 32 1019 Fragments arranged by size: 1019 811 224 32 32 HincII GTy'rAC Cuts at: 0 639 2118 Size: 639 1479 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1355 2118 Size: 1355 763 MslI CAynn'nnrTG Cuts at: 0 792 2118 Size: 792 1326 MspA1I CmG'CkG Cuts at: 0 88 611 2118 Size: 88 523 1507 Fragments arranged by size: 1507 523 88 NspI r_CATG'y Cuts at: 0 354 431 440 797 971 2118 Size: 354 77 9 357 174 1147 Fragments arranged by size: 1147 357 354 174 77 9 PciI A'CATG_T Cuts at: 0 350 427 2118 Size: 350 77 1691 Fragments arranged by size: 1691 350 77 PflMI CCAn_nnn'nTGG Cuts at: 0 805 2118 Size: 805 1313 PsiI TTA'TAA Cuts at: 0 1796 2118 Size: 1796 322 PspOMI G'GGCC_C Cuts at: 0 51 260 2118 Size: 51 209 1858 Fragments arranged by size: 1858 209 51 PvuII CAG'CTG Cuts at: 0 88 611 2118 Size: 88 523 1507 Fragments arranged by size: 1507 523 88 SacI G_AGCT'C Cuts at: 0 161 547 745 2118 Size: 161 386 198 1373 Fragments arranged by size: 1373 386 198 161 SapI GCTCTTCn'nnn_ Cuts at: 0 97 535 733 1137 1305 2118 Size: 97 438 198 404 168 813 Fragments arranged by size: 813 438 404 198 168 97 SexAI A'CCwGG_T Cuts at: 0 1922 2118 Size: 1922 196 SfcI C'TryA_G Cuts at: 0 284 2118 Size: 284 1834 SmlI C'TyrA_G Cuts at: 0 211 2118 Size: 211 1907 SspI AAT'ATT Cuts at: 0 952 2118 Size: 952 1166 StuI AGG'CCT Cuts at: 0 895 1119 1539 1707 1875 2118 Size: 895 224 420 168 168 243 Fragments arranged by size: 895 420 243 224 168 168 StyI C'CwwG_G Cuts at: 0 834 2118 Size: 834 1284 TatI w'GTAC_w Cuts at: 0 1521 2118 Size: 1521 597 XmnI GAAnn'nnTTC Cuts at: 0 571 703 979 1063 1567 2071 2118 Size: 571 132 276 84 504 504 47 Fragments arranged by size: 571 504 504 276 132 84 47 Enzymes that do cut: AccI AcuI AflIII AlwNI ApaI ApoI BamHI BanI BanII BbvCI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BsrDI BstEII BstYI BstZ17I BtsI CspCI DraIII EarI EcoICRI Eco57MI EcoO109I EcoRI Hin4I HincII MmeI MslI MspA1I NspI PciI PflMI PsiI PspOMI PvuII SacI SapI SexAI SfcI SmlI SspI StuI StyI TatI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BbeI BbsI BcgI BciVI BclI BfrBI BglI BmgBI BmtI BsaI BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI Bsu36I BtgI BtgZI ClaI DraI DrdI EaeI EagI EciI EcoNI EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PfoI PmeI PmlI PpiI PpuMI PshAI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI ZraI