################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## Acc65I AccI AccII AccIII AflII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspI AvaI AvaII AviII AvrII BbeI BbrPI BbvCI BcgI BfrI BlnI BlpI Bpu1102I BsaAI BsaI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI FauI FseI FspI HaeII HgaI HhaI HinP1I HpaI KasI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PstI PvuI PvuII RcaI RsrII SacII SalI SapI Sau96I SbfI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspBI StuI SwaI Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 1219 GACGTC GACGT^C AclI 1297 AACGTT AA^CGTT AcyI 1219 GACGTC G[GA]^CG[CT]C AhdI 2507 GACCTTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 1749 ATTAAT AT^TAAT AsnI 1749 ATTAAT AT^TAAT AspEI 2507 GACCTTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 1922 TGGCCA TGG^CCA BamHI 2788 GGATCC G^GATCC BanI 2017 GGCACC G^G[CT][GA]CC BanII 728 GAGCTC G[GA]GC[CT]^C BciVI 1861 GTATCC GTATCC(6/5) BclI 2859 TGATCA T^GATCA BcnI 594 CCCGG CC^[GC]GG BglI 2834 GCCAAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1486 AGATCT A^GATCT BsaBI 2854 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1219 GACGTC G[GA]^CG[CT]C BsaWI 2168 ACCGGA [AT]^CCGG[AT] BsgI 640 CTGCAC GTGCAG(16/14) BsrBI 1258 GAGCGG CCGCTC(-3/-3) BssSI 1526 CACGAG CACGAG(-5/-1) BstBI 3378 TTCGAA TT^CGAA BstXI 1390 CCAGAAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EaeI 1922 TGGCCA [CT]^GGCC[GA] Eam1105I 2507 GACCTTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 2190 CCTTGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 1992 ATGCAT ATGCA^T FbaI 2859 TGATCA T^GATCA Hin1I 1219 GACGTC G[GA]^CG[CT]C HincII 2164 GTCAAC GT[CT]^[GA]AC HindII 2164 GTCAAC GT[CT]^[GA]AC MamI 2854 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1922 TGGCCA TGG^CCA MscI 1922 TGGCCA TGG^CCA MslI 1240 CACAGTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 2012 CCGCTG C[AC]G^C[GT]G NciI 594 CCCGG CC^[GC]GG NsiI 1992 ATGCAT ATGCA^T NspV 3378 TTCGAA TT^CGAA PshBI 1749 ATTAAT AT^TAAT Psp1406I 1297 AACGTT AA^CGTT SacI 728 GAGCTC GAGCT^C SfuI 3378 TTCGAA TT^CGAA SmlI 1881 CTCAAG C^T[CT][GA]AG SphI 3131 GCATGC GCATG^C XcmI 2387 CCAAAGAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AlwNI 974 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG 1541 CAGATACTG BbsI 992 GTCTTC GAAGAC(2/6) 1776 GTCTTC BpmI 41 CTCCAG CTGGAG(16/14) 980 CTGGAG BpuAI 992 GTCTTC GAAGAC(2/6) 1776 GTCTTC DsaI 74 CCATGG C^C[GA][CT]GG 2084 CCATGG EcoRV 271 GATATC GAT^ATC 1349 GATATC HaeIII 1923 GGCC GG^CC 2833 GGCC HapII 595 CCGG C^CGG 2169 CCGG HindIII 1087 AAGCTT A^AGCTT 1940 AAGCTT HpaII 595 CCGG C^CGG 2169 CCGG MspI 595 CCGG C^CGG 2169 CCGG NcoI 74 CCATGG C^CATGG 2084 CCATGG PleI 858 GACTC GAGTC(4/5) 1618 GAGTC ScaI 363 AGTACT AGT^ACT 865 AGTACT SexAI 2613 ACCTGGT A^CC[AT]GGT 2697 ACCTGGT TaqI 2736 TCGA T^CGA 3379 TCGA TthHB8I 2736 TCGA T^CGA 3379 TCGA XbaI 564 TCTAGA T^CTAGA 1969 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI ApoI Asp700I AspHI BbvI BfaI BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspLU11I BsrI BssKI BstF5I BstNI BstYI Cac8I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV NspI RsaI Sau3AI ScrFI SfaNI SfcI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII XmnI