(Circular) (Six-base) MAPSORT of: pFN21AA1903.seq Check: 3767 from: 1 to: 8101 pFN21AA1903 8101 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3228 5911 278 Size: 53 83 186 936 1692 2683 2468 Fragments arranged by size: 2683 2468 1692 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5444 1937 Size: 3507 4594 Acc65I G'GTAC_C Cuts at: 5422 5422 Size: 8101 AclI AA'CG_TT Cuts at: 1563 3303 6228 6601 1563 Size: 1740 2925 373 3063 Fragments arranged by size: 3063 2925 1740 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2094 3623 3873 4383 4488 4517 4770 6164 Size: 462 1529 250 510 105 29 253 1394 Cuts at: 6164 7548 7816 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1529 1394 1384 510 462 268 253 250 105 29 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7904 AflIII A'CryG_T Cuts at: 1203 2802 3020 3648 4804 7000 7977 1203 Size: 1599 218 628 1156 2196 977 1327 Fragments arranged by size: 2196 1599 1327 1156 977 628 218 AgeI A'CCGG_T Cuts at: 6932 6932 Size: 8101 AhdI GACnn_n'nnGTC Cuts at: 4517 6830 4517 Size: 2313 5788 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8101 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8101 AlwNI CAG_nnn'CTG Cuts at: 2984 3551 5844 7416 2984 Size: 567 2293 1572 3669 Fragments arranged by size: 3669 2293 1572 567 ApaLI G'TGCA_C Cuts at: 6158 7314 6158 Size: 1156 6945 ApoI r'AATT_y Cuts at: 1493 2034 2692 2751 3333 3377 4484 4931 5410 Size: 541 658 59 582 44 1107 447 479 Cuts at: 5410 5636 5788 7897 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1107 658 582 541 479 447 226 152 59 44 AseI AT'TA_AT Cuts at: 160 1032 3755 6655 160 Size: 872 2723 2900 1606 Fragments arranged by size: 2900 2723 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8101 AvaI C'yCGr_G Cuts at: 1870 1943 5426 1870 Size: 73 3483 4545 Fragments arranged by size: 4545 3483 73 BamHI G'GATC_C Cuts at: 4793 5431 5798 4793 Size: 638 367 7096 Fragments arranged by size: 7096 638 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4022 5422 6877 618 Size: 325 217 627 2235 1400 1455 1842 Fragments arranged by size: 2235 1842 1455 1400 627 325 217 BanII G_rGCy'C Cuts at: 1391 2737 5420 1391 Size: 1346 2683 4072 Fragments arranged by size: 4072 2683 1346 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2990 3774 928 Size: 971 1091 784 5255 Fragments arranged by size: 5255 1091 971 784 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6292 6326 1903 Size: 34 4355 34 3678 Fragments arranged by size: 4355 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3877 5993 7203 3877 Size: 2116 1210 4775 Fragments arranged by size: 4775 2116 1210 BclI T'GATC_A Cuts at: 1548 4864 1548 Size: 3316 4785 BfrBI ATG'CAT Cuts at: 3999 3999 Size: 8101 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4845 6712 136 Size: 107 122 71 631 605 3173 1867 1525 Fragments arranged by size: 3173 1867 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3491 8096 3491 Size: 4605 3496 BlpI GC'TnA_GC Cuts at: 5518 5518 Size: 8101 Bme1580I G_kGCm'C Cuts at: 1790 6162 7318 1790 Size: 4372 1156 2573 Fragments arranged by size: 4372 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8101 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5832 6790 442 Size: 416 53 83 399 24 4415 958 1753 Fragments arranged by size: 4415 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8101 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2031 3006 6761 1239 Size: 792 975 3755 2579 Fragments arranged by size: 3755 2579 975 792 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3871 6226 7091 7389 3871 Size: 2355 865 298 4583 Fragments arranged by size: 4583 2355 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6764 882 Size: 617 5265 2219 Fragments arranged by size: 5265 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5892 493 Size: 711 4688 2702 Fragments arranged by size: 4688 2702 711 BsaBI GATnn'nnATC Cuts at: 4863 8095 4863 Size: 3232 4869 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3225 5908 6290 6925 Size: 53 83 186 936 1692 2683 382 635 Cuts at: 6925 275 Size: 1451 Fragments arranged by size: 2683 1692 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4173 6534 6932 7206 7353 7764 4173 Size: 2361 398 274 147 411 4510 Fragments arranged by size: 4510 2361 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2107 2137 2434 2464 1178 Size: 30 165 30 704 30 297 30 6815 Fragments arranged by size: 6815 704 297 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2146 3090 3741 3744 5100 1186 Size: 960 944 651 3 1356 4187 Fragments arranged by size: 4187 1356 960 944 651 3 BseYI C'CCAG_C Cuts at: 7304 7800 7304 Size: 496 7605 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2630 1163 Size: 573 894 6634 Fragments arranged by size: 6634 894 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5551 6312 7340 1540 Size: 275 186 3550 761 1028 2301 Fragments arranged by size: 3550 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2737 3276 3414 4424 6162 6247 7318 2737 Size: 539 138 1010 1738 85 1071 3520 Fragments arranged by size: 3520 1738 1071 1010 539 138 85 BsmI GAATG_Cn' Cuts at: 2103 4616 4760 5612 5705 2103 Size: 2513 144 852 93 4499 Fragments arranged by size: 4499 2513 852 144 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2737 3276 3414 4424 5420 6162 6247 Size: 399 947 539 138 1010 996 742 85 Cuts at: 6247 7318 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1010 996 947 742 539 399 138 85 BspEI T'CCGG_A Cuts at: 7764 7764 Size: 8101 BspHI T'CATG_A Cuts at: 5990 7720 5990 Size: 1730 6371 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5456 844 Size: 4612 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3265 5988 1394 Size: 429 1442 2723 3507 Fragments arranged by size: 3507 2723 1442 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6596 6770 65 Size: 1183 5348 174 1396 Fragments arranged by size: 5348 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6745 6932 1700 Size: 110 4935 187 2869 Fragments arranged by size: 4935 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8101 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8101 BssSI C'ACGA_G Cuts at: 3531 6161 7173 3531 Size: 2630 1012 4459 Fragments arranged by size: 4459 2630 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8101 BstBI TT'CG_AA Cuts at: 5384 6905 5384 Size: 1521 6580 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8101 BstXI CCAn_nnnn'nTGG Cuts at: 3402 3402 Size: 8101 BstYI r'GATC_y Cuts at: 1972 3491 4580 4793 5431 5798 6183 6200 7652 Size: 1519 1089 213 638 367 385 17 1452 Cuts at: 7652 7738 7750 8096 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1519 1452 1089 638 385 367 346 213 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2079 4089 513 Size: 552 1014 2010 4525 Fragments arranged by size: 4525 2010 1014 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4161 487 Size: 683 56 2935 4427 Fragments arranged by size: 4427 2935 683 56 BtsI GCAGTG_nn' Cuts at: 2642 4407 5617 6411 6431 2642 Size: 1765 1210 794 20 4312 Fragments arranged by size: 4312 1765 1210 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8101 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3644 3679 529 Size: 35 3080 35 4951 Fragments arranged by size: 4951 3080 35 35 DraI TTT'AAA Cuts at: 5405 5405 Size: 8101 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7108 809 Size: 507 5792 1802 Fragments arranged by size: 5792 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3927 5548 6437 Size: 54 1425 50 275 18 2097 1621 889 Cuts at: 6437 8066 8 Size: 1629 43 Fragments arranged by size: 2097 1629 1621 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5548 1537 Size: 275 3736 4090 Fragments arranged by size: 4090 3736 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3599 4536 4692 5358 6031 1353 Size: 2246 937 156 666 673 3423 Fragments arranged by size: 3423 2246 937 673 666 156 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6688 7062 7208 387 Size: 1064 138 5099 374 146 1280 Fragments arranged by size: 5099 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2735 2735 Size: 8101 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2031 2094 3006 3623 3873 4383 4488 Size: 393 399 63 912 617 250 510 105 Cuts at: 4488 4517 4770 6164 6761 7548 7816 1239 Size: 29 253 1394 597 787 268 1524 Fragments arranged by size: 1524 1394 912 787 617 597 510 399 393 268 253 250 105 63 29 EcoNI CCTnn'n_nnAGG Cuts at: 4199 4199 Size: 8101 EcoRI G'AATT_C Cuts at: 5410 5788 5410 Size: 378 7723 EcoRV GAT'ATC Cuts at: 2278 3356 2278 Size: 1078 7023 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4547 4547 Size: 8101 FspI TGC'GCA Cuts at: 6607 6607 Size: 8101 HaeII r_GCGC'y Cuts at: 1678 7248 1678 Size: 5570 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2538 2570 3725 3757 3994 Size: 32 135 32 966 32 1155 32 237 Cuts at: 3994 4026 4503 4535 6742 6774 6816 6848 1373 Size: 32 477 32 2207 32 42 32 2626 Fragments arranged by size: 2626 2207 1155 966 477 237 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4171 5445 5691 669 Size: 988 281 2233 1274 246 3079 Fragments arranged by size: 3079 2233 1274 988 281 246 HindIII A'AGCT_T Cuts at: 748 3092 3945 748 Size: 2344 853 4904 Fragments arranged by size: 4904 2344 853 HpaI GTT'AAC Cuts at: 5691 5691 Size: 8101 KpnI G_GTAC'C Cuts at: 5426 5426 Size: 8101 MfeI C'AATT_G Cuts at: 5700 5700 Size: 8101 MluI A'CGCG_T Cuts at: 7977 7977 Size: 8101 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2394 2693 5093 5291 7215 7399 1305 Size: 1089 299 2400 198 1924 184 2007 Fragments arranged by size: 2400 2007 1924 1089 299 198 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3929 10 Size: 54 1425 343 2097 4182 Fragments arranged by size: 4182 2097 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3249 6059 6418 6577 518 Size: 648 2083 2810 359 159 2042 Fragments arranged by size: 2810 2083 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4019 5517 6194 7342 7587 1606 Size: 2413 1498 677 1148 245 2120 Fragments arranged by size: 2413 2120 1498 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2079 4089 513 Size: 552 1014 2010 4525 Fragments arranged by size: 4525 2010 1014 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8101 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8101 NotI GC'GGCC_GC Cuts at: 5548 5548 Size: 8101 NsiI A_TGCA'T Cuts at: 4001 4001 Size: 8101 NspI r_CATG'y Cuts at: 1132 1229 3024 3652 4808 5126 5140 5459 7004 Size: 97 1795 628 1156 318 14 319 1545 Cuts at: 7004 1132 Size: 2229 Fragments arranged by size: 2229 1795 1545 1156 628 319 318 97 14 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8101 PciI A'CATG_T Cuts at: 3020 3648 4804 7000 3020 Size: 628 1156 2196 4121 Fragments arranged by size: 4121 2196 1156 628 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8101 PfoI T'CCnGG_A Cuts at: 5019 5019 Size: 8101 PmeI GTTT'AAAC Cuts at: 5405 5405 Size: 8101 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4197 4229 6170 6202 7683 7715 4197 Size: 32 1941 32 1481 32 4583 Fragments arranged by size: 4583 1941 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8101 PsiI TTA'TAA Cuts at: 4293 5671 4293 Size: 1378 6723 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4143 4175 4143 Size: 32 8069 PstI C_TGCA'G Cuts at: 830 5401 5453 830 Size: 4571 52 3478 Fragments arranged by size: 4571 3478 52 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8101 SacI G_AGCT'C Cuts at: 2737 2737 Size: 8101 SalI G'TCGA_C Cuts at: 1936 5443 1936 Size: 3507 4594 SbfI CC_TGCA'GG Cuts at: 5453 5453 Size: 8101 ScaI AGT'ACT Cuts at: 2370 2872 6349 8061 2370 Size: 502 3477 1712 2410 Fragments arranged by size: 3477 2410 1712 502 SexAI A'CCwGG_T Cuts at: 4618 4702 4618 Size: 84 8017 SfcI C'TryA_G Cuts at: 826 1045 2703 3158 3636 4731 5397 5449 6584 Size: 219 1658 455 478 1095 666 52 1135 Cuts at: 6584 7265 7456 826 Size: 681 191 1471 Fragments arranged by size: 1658 1471 1135 1095 681 666 478 455 219 191 52 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8101 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8101 SmaI CCC'GGG Cuts at: 1872 5428 1872 Size: 3556 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3886 6205 7106 7368 820 Size: 197 926 1943 2319 901 262 1553 Fragments arranged by size: 2319 1943 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5892 493 Size: 5399 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8101 SphI G_CATG'C Cuts at: 1132 5140 5459 1132 Size: 4008 319 3774 Fragments arranged by size: 4008 3774 319 SspI AAT'ATT Cuts at: 5 52 2346 3085 3111 4441 6025 5 Size: 47 2294 739 26 1330 1584 2081 Fragments arranged by size: 2294 2081 1584 1330 739 47 26 StuI AGG'CCT Cuts at: 1517 1517 Size: 8101 StyI C'CwwG_G Cuts at: 513 1065 2079 2557 4089 5540 513 Size: 552 1014 478 1532 1451 3074 Fragments arranged by size: 3074 1532 1451 1014 552 478 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6128 6145 6298 6483 6928 325 Size: 1255 4548 17 153 185 445 1498 Fragments arranged by size: 4548 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2368 2870 3031 Size: 274 80 33 51 1443 391 502 161 Cuts at: 3031 4976 6347 8059 96 Size: 1945 1371 1712 138 Fragments arranged by size: 1945 1712 1443 1371 502 391 274 161 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2107 2139 2964 2996 5149 5181 8061 8093 2107 Size: 32 825 32 2153 32 2880 32 2115 Fragments arranged by size: 2880 2153 2115 825 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8101 XbaI T'CTAG_A Cuts at: 2569 3974 5437 2569 Size: 1405 1463 5233 Fragments arranged by size: 5233 1463 1405 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4399 4399 Size: 8101 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8101 XmaI C'CCGG_G Cuts at: 1870 5426 1870 Size: 3556 4545 XmnI GAAnn'nnTTC Cuts at: 2189 2841 3085 4530 6230 2189 Size: 652 244 1445 1700 4060 Fragments arranged by size: 4060 1700 1445 652 244 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3226 5909 276 Size: 53 83 186 936 1692 2683 2468 Fragments arranged by size: 2683 2468 1692 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI