Restriction map for
pFN21AB0444
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspI AviII AvrII
BamHI BanII BbvCI BcgI BciVI
BfrI BlnI BlpI Bpu1102I BsaI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BspHI BspLU11I BsrBI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I CelII ClaI CpoI
DrdI EagI EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI FseI
FspI HgaI HpaI KpnI Ksp632I
KspI MluI MroI MslI MvnI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI Tth111I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AhdI 2247 GACTAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1717 CCTGAGG CC^T[ACGT]AGG
AspEI 2247 GACTAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1245 GAGCAC G[AT]GC[AT]^C
AvaI 1057 CTCGAG C^[CT]CG[GA]G
BbrPI 1348 CACGTG CAC^GTG
BclI 1733 TGATCA T^GATCA
BglII 2358 AGATCT A^GATCT
BpmI 35 CTCCAG CTGGAG(16/14)
BsaAI 1348 CACGTG [CT]AC^GT[GA]
BsaBI 1424 GATACTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 1245 GAGCAC G[AT]GC[AT]^C
BsmBI 2485 GAGACG CGTCTC(1/5)
BsmI 2277 GAATGC GAATGC(1/-1)
BsoBI 1057 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1700 TGTACA T^GTACA
BsrDI 1785 CATTGC GCAATG(2/0)
BsrFI 2702 GCCGGT [GA]^CCGG[CT]
BsrGI 1700 TGTACA T^GTACA
BssSI 1199 CACGAG CACGAG(-5/-1)
BstXI 2681 CCAGTACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 2358 AGATCT [GA]^GATC[CT]
Bsu36I 1717 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2702 GCCGGT [GA]^CCGG[CT]
DraI 1579 TTTAAA TTT^AAA
DsaI 1955 CCGTGG C^C[GA][CT]GG
Eam1105I 2247 GACTAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1717 CCTGAGG CC^T[ACGT]AGG
EcoRV 1712 GATATC GAT^ATC
EcoT22I 2658 ATGCAT ATGCA^T
FbaI 1733 TGATCA T^GATCA
HincII 1837 GTCAAC GT[CT]^[GA]AC
HindII 1837 GTCAAC GT[CT]^[GA]AC
HindIII 1177 AAGCTT A^AGCTT
MamI 1424 GATACTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2129 CAATTG C^AATTG
MflI 2358 AGATCT [GA]^GATC[CT]
MunI 2129 CAATTG C^AATTG
NsiI 2658 ATGCAT ATGCA^T
NspI 453 ACATGC [GA]CATG^[CT]
PaeR7I 1057 CTCGAG C^TCGAG
PflMI 2192 CCAGAAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1348 CACGTG CAC^GTG
PmlI 1348 CACGTG CAC^GTG
PpuMI 801 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 1403 CAGCTG CAG^CTG
ScaI 2683 AGTACT AGT^ACT
SspBI 1700 TGTACA T^GTACA
SspI 331 AATATT AAT^ATT
StuI 1328 AGGCCT AGG^CCT
Tsp45I 450 GTCAC ^GT[GC]AC
Van91I 2192 CCAGAAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1893 CCATTCTCTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1057 CTCGAG C^TCGAG
XhoII 2358 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AvaII 604 GGTCC G^G[AT]CC
802 GGACC
BbvI 2560 GCAGC GCAGC(8/12)
523 GCTGC
BglI 1330 GCCTGTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2281 GCCCTGCGGGC
BseRI 117 CTCCTC GAGGAG(10/8)
583 GAGGAG
BsgI 475 CTGCAC GTGCAG(16/14)
1685 GTGCAG
DraII 801 AGGACCT [GA]G^G[ACGT]CC[CT]
1306 AGGCCCC
DraIII 1390 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
1586 CACAAGGTG
EcoO109I 801 AGGACCT [GA]G^G[ACGT]CC[CT]
1306 AGGCCCC
FauI 543 GCGGG CCCGC(4/6)
2286 GCGGG
Fnu4HI 523 GCTGC GC^[ACGT]GC
2560 GCAGC
ItaI 523 GCTGC GC^[ACGT]GC
2560 GCAGC
MspA1I 541 CAGCGG C[AC]G^C[GT]G
1403 CAGCTG
SmlI 1057 CTCGAG C^T[CT][GA]AG
1963 CTCAAG
TseI 523 GCTGC G^C[AT]GC
2560 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI Asp700I BalI
BanI BbeI BbsI BcnI BfaI
BmyI BpuAI BsaHI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EcoRII EcoT14I FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI KasI MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MspI MvaI MwoI
NarI NciI NdeII NlaIII NlaIV
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI StyI
TaqI TfiI Tru9I Tsp509I TspRI
TthHB8I XmnI