(Circular) (Six-base) MAPSORT of: pFN21AB0444.seq Check: 90 from: 1 to: 7420 pFN21AB0444 7420 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2294 2989 2294 Size: 695 6725 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5230 278 Size: 53 83 186 936 3694 2468 Fragments arranged by size: 3694 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4763 1937 Size: 2826 4594 Acc65I G'GTAC_C Cuts at: 4741 4741 Size: 7420 AclI AA'CG_TT Cuts at: 1563 5547 5920 1563 Size: 3984 373 3063 Fragments arranged by size: 3984 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2736 2907 3099 3432 3482 4082 4529 5483 Size: 1104 171 192 333 50 600 447 954 Cuts at: 5483 6867 7135 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1104 954 600 447 333 268 192 171 50 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7223 AflIII A'CryG_T Cuts at: 1203 6319 7296 1203 Size: 5116 977 1327 Fragments arranged by size: 5116 1327 977 AgeI A'CCGG_T Cuts at: 6251 6251 Size: 7420 AhdI GACnn_n'nnGTC Cuts at: 4257 6149 4257 Size: 1892 5528 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7420 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3371 1184 Size: 2187 5233 AlwNI CAG_nnn'CTG Cuts at: 2048 2220 2642 2981 5163 6735 2048 Size: 172 422 339 2182 1572 2733 Fragments arranged by size: 2733 2182 1572 422 339 172 ApaLI G'TGCA_C Cuts at: 5477 6633 5477 Size: 1156 6264 ApoI r'AATT_y Cuts at: 1493 2109 2435 2864 3032 3581 3608 4233 4412 Size: 616 326 429 168 549 27 625 179 Cuts at: 4412 4729 4955 5107 7216 1493 Size: 317 226 152 2109 1697 Fragments arranged by size: 2109 1697 625 616 549 429 326 317 226 179 168 152 27 AseI AT'TA_AT Cuts at: 160 1032 5974 160 Size: 872 4942 1606 Fragments arranged by size: 4942 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7420 AvaI C'yCGr_G Cuts at: 1870 1943 3062 4745 1870 Size: 73 1119 1683 4545 Fragments arranged by size: 4545 1683 1119 73 BamHI G'GATC_C Cuts at: 4750 5117 4750 Size: 367 7053 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3281 3364 4657 4741 6196 Size: 325 217 627 1494 83 1293 84 1455 Cuts at: 6196 618 Size: 1842 Fragments arranged by size: 1842 1494 1455 1293 627 325 217 84 83 BanII G_rGCy'C Cuts at: 1391 4739 1391 Size: 3348 4072 BbeI G_GCGC'C Cuts at: 3285 3368 4661 3285 Size: 83 1293 6044 Fragments arranged by size: 6044 1293 83 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3666 3929 4617 928 Size: 971 1767 263 688 3731 Fragments arranged by size: 3731 1767 971 688 263 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5611 5645 1903 Size: 34 3674 34 3678 Fragments arranged by size: 3678 3674 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5312 6522 5312 Size: 1210 6210 BclI T'GATC_A Cuts at: 1548 3738 1548 Size: 2190 5230 BfrBI ATG'CAT Cuts at: 4665 4665 Size: 7420 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3341 4292 6031 Size: 107 122 71 631 605 1669 951 1739 Cuts at: 6031 136 Size: 1525 Fragments arranged by size: 1739 1669 1525 951 631 605 122 107 71 BglII A'GATC_T Cuts at: 4363 7415 4363 Size: 3052 4368 BlpI GC'TnA_GC Cuts at: 4837 4837 Size: 7420 Bme1580I G_kGCm'C Cuts at: 1790 3205 3234 5481 6637 1790 Size: 1415 29 2247 1156 2573 Fragments arranged by size: 2573 2247 1415 1156 29 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7420 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3228 4366 5151 Size: 416 53 83 399 24 1811 1138 785 Cuts at: 5151 6109 442 Size: 958 1753 Fragments arranged by size: 1811 1753 1138 958 785 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7420 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2025 6080 1239 Size: 786 4055 2579 Fragments arranged by size: 4055 2579 786 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3953 5545 6410 6708 3953 Size: 1592 865 298 4665 Fragments arranged by size: 4665 1592 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6083 882 Size: 617 4584 2219 Fragments arranged by size: 4584 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3355 5211 493 Size: 711 2151 1856 2702 Fragments arranged by size: 2702 2151 1856 711 BsaBI GATnn'nnATC Cuts at: 3433 7414 3433 Size: 3981 3439 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3282 3365 4658 5227 Size: 53 83 186 936 1749 83 1293 569 Cuts at: 5227 5609 6244 275 Size: 382 635 1451 Fragments arranged by size: 1749 1451 1293 936 635 569 382 186 83 83 53 BsaWI w'CCGG_w Cuts at: 5853 6251 6525 6672 7083 5853 Size: 398 274 147 411 6190 Fragments arranged by size: 6190 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7195 Fragments arranged by size: 7195 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2113 2603 1186 Size: 927 490 6003 Fragments arranged by size: 6003 927 490 BseYI C'CCAG_C Cuts at: 2763 6623 7119 2763 Size: 3860 496 3064 Fragments arranged by size: 3860 3064 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2465 3711 1163 Size: 573 729 1246 4872 Fragments arranged by size: 4872 1246 729 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4870 5631 6659 1540 Size: 275 186 2869 761 1028 2301 Fragments arranged by size: 2869 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3254 5481 5566 6637 3254 Size: 2227 85 1071 4037 Fragments arranged by size: 4037 2227 1071 85 BsmI GAATG_Cn' Cuts at: 4288 4931 5024 4288 Size: 643 93 6684 Fragments arranged by size: 6684 643 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4484 4484 Size: 7420 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3205 3234 3254 4739 5481 5566 6637 Size: 399 1415 29 20 1485 742 85 1071 Cuts at: 6637 1391 Size: 2174 Fragments arranged by size: 2174 1485 1415 1071 742 399 85 29 20 BspEI T'CCGG_A Cuts at: 7083 7083 Size: 7420 BspHI T'CATG_A Cuts at: 5309 7039 5309 Size: 1730 5690 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2294 2989 3388 3849 4775 844 Size: 1450 695 399 461 926 3489 Fragments arranged by size: 3489 1450 926 695 461 399 BsrBI CCG'CTC Cuts at: 1394 1823 5307 1394 Size: 429 3484 3507 Fragments arranged by size: 3507 3484 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3789 5915 6089 65 Size: 1183 2541 2126 174 1396 Fragments arranged by size: 2541 2126 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4707 6064 6251 1700 Size: 110 2897 1357 187 2869 Fragments arranged by size: 2897 2869 1357 187 110 BsrGI T'GTAC_A Cuts at: 96 3705 96 Size: 3609 3811 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7420 BssSI C'ACGA_G Cuts at: 3204 5480 6492 3204 Size: 2276 1012 4132 Fragments arranged by size: 4132 2276 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7420 BstBI TT'CG_AA Cuts at: 6224 6224 Size: 7420 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7420 BstXI CCAn_nnnn'nTGG Cuts at: 4693 4693 Size: 7420 BstYI r'GATC_y Cuts at: 1972 4363 4750 5117 5502 5519 6971 7057 7069 Size: 2391 387 367 385 17 1452 86 12 Cuts at: 7069 7415 1972 Size: 346 1977 Fragments arranged by size: 2391 1977 1452 387 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3723 3723 Size: 7420 BtgI C'CryG_G Cuts at: 513 1065 2004 3960 513 Size: 552 939 1956 3973 Fragments arranged by size: 3973 1956 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6681 Fragments arranged by size: 6681 683 56 BtsI GCAGTG_nn' Cuts at: 2911 3048 3103 4217 4936 5730 5750 2911 Size: 137 55 1114 719 794 20 4581 Fragments arranged by size: 4581 1114 794 719 137 55 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7420 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4691 4726 529 Size: 35 4127 35 3223 Fragments arranged by size: 4127 3223 35 35 DraI TTT'AAA Cuts at: 3586 4724 3586 Size: 1138 6282 DraIII CAC_nnn'GTG Cuts at: 3400 3596 3400 Size: 196 7224 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6427 809 Size: 507 5111 1802 Fragments arranged by size: 5111 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2706 2877 3045 Size: 54 1425 50 275 18 876 171 168 Cuts at: 3045 4867 5756 7385 8 Size: 1822 889 1629 43 Fragments arranged by size: 1822 1629 1425 889 876 275 171 168 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4867 1537 Size: 275 3055 4090 Fragments arranged by size: 4090 3055 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5350 1353 Size: 3997 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6007 6381 6527 387 Size: 1064 138 4418 374 146 1280 Fragments arranged by size: 4418 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2025 2736 2907 3099 3432 3482 4082 Size: 393 393 711 171 192 333 50 600 Cuts at: 4082 4529 5483 6080 6867 7135 1239 Size: 447 954 597 787 268 1524 Fragments arranged by size: 1524 954 787 711 600 597 447 393 393 333 268 192 171 50 EcoO109I rG'GnC_Cy Cuts at: 2807 3312 2807 Size: 505 6915 EcoRI G'AATT_C Cuts at: 4729 5107 4729 Size: 378 7042 EcoRV GAT'ATC Cuts at: 3719 3719 Size: 7420 FspI TGC'GCA Cuts at: 5926 5926 Size: 7420 HaeII r_GCGC'y Cuts at: 1678 3285 3368 4661 6567 1678 Size: 1607 83 1293 1906 2531 Fragments arranged by size: 2531 1906 1607 1293 83 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2016 2048 3716 3748 3973 Size: 32 135 32 444 32 1668 32 225 Cuts at: 3973 4005 4243 4275 6061 6093 6135 6167 1373 Size: 32 238 32 1786 32 42 32 2626 Fragments arranged by size: 2626 1786 1668 444 238 225 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3844 4764 5010 669 Size: 988 281 1906 920 246 3079 Fragments arranged by size: 3079 1906 988 920 281 246 HindIII A'AGCT_T Cuts at: 748 3182 748 Size: 2434 4986 HpaI GTT'AAC Cuts at: 5010 5010 Size: 7420 KasI G'GCGC_C Cuts at: 3281 3364 4657 3281 Size: 83 1293 6044 Fragments arranged by size: 6044 1293 83 KpnI G_GTAC'C Cuts at: 4745 4745 Size: 7420 MfeI C'AATT_G Cuts at: 4134 5019 4134 Size: 885 6535 MluI A'CGCG_T Cuts at: 7296 7296 Size: 7420 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3174 6534 6718 1305 Size: 1869 3360 184 2007 Fragments arranged by size: 3360 2007 1869 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2708 2879 3047 10 Size: 54 1425 343 876 171 168 4383 Fragments arranged by size: 4383 1425 876 343 171 168 54 MslI CAynn'nnrTG Cuts at: 518 1166 5378 5737 5896 518 Size: 648 4212 359 159 2042 Fragments arranged by size: 4212 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2548 3410 4836 5513 6661 6906 1606 Size: 942 862 1426 677 1148 245 2120 Fragments arranged by size: 2120 1426 1148 942 862 677 245 NarI GG'CG_CC Cuts at: 3282 3365 4658 3282 Size: 83 1293 6044 Fragments arranged by size: 6044 1293 83 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5929 Fragments arranged by size: 5929 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7420 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7420 NotI GC'GGCC_GC Cuts at: 4867 4867 Size: 7420 NsiI A_TGCA'T Cuts at: 4667 4667 Size: 7420 NspI r_CATG'y Cuts at: 1132 1229 2462 4778 6323 1132 Size: 97 1233 2316 1545 2229 Fragments arranged by size: 2316 2229 1545 1233 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7420 PciI A'CATG_T Cuts at: 6319 6319 Size: 7420 PflMI CCAn_nnn'nTGG Cuts at: 1108 4203 1108 Size: 3095 4325 PmeI GTTT'AAAC Cuts at: 4724 4724 Size: 7420 PmlI CAC'GTG Cuts at: 3355 3355 Size: 7420 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5489 5521 7002 7034 5489 Size: 32 1481 32 5875 Fragments arranged by size: 5875 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2807 2807 Size: 7420 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7420 PsiI TTA'TAA Cuts at: 2988 4990 2988 Size: 2002 5418 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2394 2426 2394 Size: 32 7388 PstI C_TGCA'G Cuts at: 830 2968 3442 4567 4772 830 Size: 2138 474 1125 205 3478 Fragments arranged by size: 3478 2138 1125 474 205 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7420 PvuII CAG'CTG Cuts at: 3410 3410 Size: 7420 SalI G'TCGA_C Cuts at: 1936 4762 1936 Size: 2826 4594 SbfI CC_TGCA'GG Cuts at: 4772 4772 Size: 7420 ScaI AGT'ACT Cuts at: 4690 5668 7380 4690 Size: 978 1712 4730 Fragments arranged by size: 4730 1712 978 SfcI C'TryA_G Cuts at: 826 1045 2964 3438 4563 4768 5903 6584 6775 Size: 219 1919 474 1125 205 1135 681 191 Cuts at: 6775 826 Size: 1471 Fragments arranged by size: 1919 1471 1135 1125 681 474 219 205 191 SfoI GGC'GCC Cuts at: 3283 3366 4659 3283 Size: 83 1293 6044 Fragments arranged by size: 6044 1293 83 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7420 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7420 SmaI CCC'GGG Cuts at: 1872 4747 1872 Size: 2875 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3062 3968 5524 6425 6687 820 Size: 197 926 1119 906 1556 901 262 1553 Fragments arranged by size: 1556 1553 1119 926 906 901 262 197 SnaBI TAC'GTA Cuts at: 493 5211 493 Size: 4718 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7420 SphI G_CATG'C Cuts at: 1132 4778 1132 Size: 3646 3774 SspI AAT'ATT Cuts at: 5 52 2338 5344 5 Size: 47 2286 3006 2081 Fragments arranged by size: 3006 2286 2081 47 StuI AGG'CCT Cuts at: 1517 3335 1517 Size: 1818 5602 StyI C'CwwG_G Cuts at: 513 1065 2004 3368 3907 4296 4702 4859 513 Size: 552 939 1364 539 389 406 157 3074 Fragments arranged by size: 3074 1364 939 552 539 406 389 157 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4585 5447 5464 5617 5802 6247 325 Size: 1255 3005 862 17 153 185 445 1498 Fragments arranged by size: 3005 1498 1255 862 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3122 3155 3705 Size: 274 80 33 51 1443 1145 33 550 Cuts at: 3705 4688 5666 7378 96 Size: 983 978 1712 138 Fragments arranged by size: 1712 1443 1145 983 978 550 274 138 80 51 33 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2883 2915 3075 3107 7380 7412 2883 Size: 32 160 32 4273 32 2891 Fragments arranged by size: 4273 2891 160 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7420 XbaI T'CTAG_A Cuts at: 4756 4756 Size: 7420 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3905 3905 Size: 7420 XhoI C'TCGA_G Cuts at: 1943 3062 1943 Size: 1119 6301 XmaI C'CCGG_G Cuts at: 1870 4745 1870 Size: 2875 4545 XmnI GAAnn'nnTTC Cuts at: 3663 3833 4056 5549 3663 Size: 170 223 1493 5534 Fragments arranged by size: 5534 1493 223 170 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5228 276 Size: 53 83 186 936 3694 2468 Fragments arranged by size: 3694 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BstZ17I EcoICRI EcoNI FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI