(Linear) (Six-base) MAPSORT of: KIBB0444.seq Check: 4554 from: 1 to: 2715 KIBB0444.orf With 182 enzymes: SgfI * July 15, 2010 18:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 289 984 2715 Size: 289 695 1731 Fragments arranged by size: 1731 695 289 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 731 902 1094 1427 1477 2077 2524 2715 Size: 731 171 192 333 50 600 447 191 Fragments arranged by size: 731 600 447 333 192 191 171 50 AhdI GACnn_n'nnGTC Cuts at: 0 2252 2715 Size: 2252 463 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1366 2715 Size: 1366 1349 AlwNI CAG_nnn'CTG Cuts at: 0 43 215 637 976 2715 Size: 43 172 422 339 1739 Fragments arranged by size: 1739 422 339 172 43 ApoI r'AATT_y Cuts at: 0 104 430 859 1027 1576 1603 2228 2407 Size: 104 326 429 168 549 27 625 179 Cuts at: 2407 2715 Size: 308 Fragments arranged by size: 625 549 429 326 308 179 168 104 27 AvaI C'yCGr_G Cuts at: 0 1057 2715 Size: 1057 1658 BanI G'GyrC_C Cuts at: 0 1276 1359 2652 2715 Size: 1276 83 1293 63 Fragments arranged by size: 1293 1276 83 63 BbeI G_GCGC'C Cuts at: 0 1280 1363 2656 2715 Size: 1280 83 1293 59 Fragments arranged by size: 1293 1280 83 59 BbsI GAAGACnn'nnnn_ Cuts at: 0 1661 1924 2612 2715 Size: 1661 263 688 103 Fragments arranged by size: 1661 688 263 103 BclI T'GATC_A Cuts at: 0 1733 2715 Size: 1733 982 BfrBI ATG'CAT Cuts at: 0 2660 2715 Size: 2660 55 BglI GCCn_nnn'nGGC Cuts at: 0 1336 2287 2715 Size: 1336 951 428 Fragments arranged by size: 1336 951 428 BglII A'GATC_T Cuts at: 0 2358 2715 Size: 2358 357 Bme1580I G_kGCm'C Cuts at: 0 1200 1229 2715 Size: 1200 29 1486 Fragments arranged by size: 1486 1200 29 BmrI ACTGGGnnnn_n' Cuts at: 0 1223 2361 2715 Size: 1223 1138 354 Fragments arranged by size: 1223 1138 354 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 20 2715 Size: 20 2695 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1948 2715 Size: 1948 767 BsaAI yAC'GTr Cuts at: 0 1350 2715 Size: 1350 1365 BsaBI GATnn'nnATC Cuts at: 0 1428 2715 Size: 1428 1287 BsaHI Gr'CG_yC Cuts at: 0 1277 1360 2653 2715 Size: 1277 83 1293 62 Fragments arranged by size: 1293 1277 83 62 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 108 598 2715 Size: 108 490 2117 Fragments arranged by size: 2117 490 108 BseYI C'CCAG_C Cuts at: 0 758 2715 Size: 758 1957 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 460 1706 2715 Size: 460 1246 1009 Fragments arranged by size: 1246 1009 460 BsiHKAI G_wGCw'C Cuts at: 0 1249 2715 Size: 1249 1466 BsmI GAATG_Cn' Cuts at: 0 2283 2715 Size: 2283 432 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2479 2715 Size: 2479 236 Bsp1286I G_dGCh'C Cuts at: 0 1200 1229 1249 2715 Size: 1200 29 20 1466 Fragments arranged by size: 1466 1200 29 20 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 289 984 1383 1844 2715 Size: 289 695 399 461 871 Fragments arranged by size: 871 695 461 399 289 BsrDI GCAATG_nn' Cuts at: 0 1784 2715 Size: 1784 931 BsrFI r'CCGG_y Cuts at: 0 2702 2715 Size: 2702 13 BsrGI T'GTAC_A Cuts at: 0 1700 2715 Size: 1700 1015 BssSI C'ACGA_G Cuts at: 0 1199 2715 Size: 1199 1516 BstXI CCAn_nnnn'nTGG Cuts at: 0 2688 2715 Size: 2688 27 BstYI r'GATC_y Cuts at: 0 2358 2715 Size: 2358 357 Bsu36I CC'TnA_GG Cuts at: 0 1718 2715 Size: 1718 997 BtgI C'CryG_G Cuts at: 0 1955 2715 Size: 1955 760 BtsI GCAGTG_nn' Cuts at: 0 906 1043 1098 2212 2715 Size: 906 137 55 1114 503 Fragments arranged by size: 1114 906 503 137 55 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2686 2715 Size: 2686 29 DraI TTT'AAA Cuts at: 0 1581 2715 Size: 1581 1134 DraIII CAC_nnn'GTG Cuts at: 0 1395 1591 2715 Size: 1395 196 1124 Fragments arranged by size: 1395 1124 196 EaeI y'GGCC_r Cuts at: 0 701 872 1040 2715 Size: 701 171 168 1675 Fragments arranged by size: 1675 701 171 168 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 20 731 902 1094 1427 1477 2077 2524 Size: 20 711 171 192 333 50 600 447 Cuts at: 2524 2715 Size: 191 Fragments arranged by size: 711 600 447 333 192 191 171 50 20 EcoO109I rG'GnC_Cy Cuts at: 0 802 1307 2715 Size: 802 505 1408 Fragments arranged by size: 1408 802 505 EcoRV GAT'ATC Cuts at: 0 1714 2715 Size: 1714 1001 HaeII r_GCGC'y Cuts at: 0 1280 1363 2656 2715 Size: 1280 83 1293 59 Fragments arranged by size: 1293 1280 83 59 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 11 43 1711 1743 1968 2000 2238 2270 Size: 11 32 1668 32 225 32 238 32 Cuts at: 2270 2715 Size: 445 Fragments arranged by size: 1668 445 238 225 32 32 32 32 11 HincII GTy'rAC Cuts at: 0 1839 2715 Size: 1839 876 HindIII A'AGCT_T Cuts at: 0 1177 2715 Size: 1177 1538 KasI G'GCGC_C Cuts at: 0 1276 1359 2652 2715 Size: 1276 83 1293 63 Fragments arranged by size: 1293 1276 83 63 MfeI C'AATT_G Cuts at: 0 2129 2715 Size: 2129 586 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1169 2715 Size: 1169 1546 MscI TGG'CCA Cuts at: 0 703 874 1042 2715 Size: 703 171 168 1673 Fragments arranged by size: 1673 703 171 168 MspA1I CmG'CkG Cuts at: 0 543 1405 2715 Size: 543 862 1310 Fragments arranged by size: 1310 862 543 NarI GG'CG_CC Cuts at: 0 1277 1360 2653 2715 Size: 1277 83 1293 62 Fragments arranged by size: 1293 1277 83 62 NsiI A_TGCA'T Cuts at: 0 2662 2715 Size: 2662 53 NspI r_CATG'y Cuts at: 0 457 2715 Size: 457 2258 PflMI CCAn_nnn'nTGG Cuts at: 0 2198 2715 Size: 2198 517 PmlI CAC'GTG Cuts at: 0 1350 2715 Size: 1350 1365 PpuMI rG'GwC_Cy Cuts at: 0 802 2715 Size: 802 1913 PsiI TTA'TAA Cuts at: 0 983 2715 Size: 983 1732 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 389 421 2715 Size: 389 32 2294 Fragments arranged by size: 2294 389 32 PstI C_TGCA'G Cuts at: 0 963 1437 2562 2715 Size: 963 474 1125 153 Fragments arranged by size: 1125 963 474 153 PvuII CAG'CTG Cuts at: 0 1405 2715 Size: 1405 1310 ScaI AGT'ACT Cuts at: 0 2685 2715 Size: 2685 30 SfcI C'TryA_G Cuts at: 0 959 1433 2558 2715 Size: 959 474 1125 157 Fragments arranged by size: 1125 959 474 157 SfoI GGC'GCC Cuts at: 0 1278 1361 2654 2715 Size: 1278 83 1293 61 Fragments arranged by size: 1293 1278 83 61 SmlI C'TyrA_G Cuts at: 0 1057 1963 2715 Size: 1057 906 752 Fragments arranged by size: 1057 906 752 SspI AAT'ATT Cuts at: 0 333 2715 Size: 333 2382 StuI AGG'CCT Cuts at: 0 1330 2715 Size: 1330 1385 StyI C'CwwG_G Cuts at: 0 1363 1902 2291 2697 2715 Size: 1363 539 389 406 18 Fragments arranged by size: 1363 539 406 389 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2580 2715 Size: 2580 135 TatI w'GTAC_w Cuts at: 0 1117 1150 1700 2683 2715 Size: 1117 33 550 983 32 Fragments arranged by size: 1117 983 550 33 32 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 878 910 1070 1102 2715 Size: 878 32 160 32 1613 Fragments arranged by size: 1613 878 160 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1900 2715 Size: 1900 815 XhoI C'TCGA_G Cuts at: 0 1057 2715 Size: 1057 1658 XmnI GAAnn'nnTTC Cuts at: 0 1658 1828 2051 2715 Size: 1658 170 223 664 Fragments arranged by size: 1658 664 223 170 Enzymes that do cut: AarI AcuI AhdI AlfI AlwNI ApoI AvaI BanI BbeI BbsI BclI BfrBI BglI BglII Bme1580I BmrI BpmI BpuEI BsaAI BsaBI BsaHI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BsrFI BsrGI BssSI BstXI BstYI Bsu36I BtgI BtsI CspCI DraI DraIII EaeI Eco57MI EcoO109I EcoRV HaeII Hin4I HincII HindIII KasI MfeI MmeI MscI MspA1I NarI NsiI NspI PflMI PmlI PpuMI PsiI PsrI PstI PvuII ScaI SfcI SfoI SmlI SspI StuI StyI TaqII TatI TstI XcmI XhoI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanII BbvCI BcgI BciVI BlpI BmgBI BmtI BplI Bpu10I BsaI BsaWI BsaXI BsiEI BsiWI BspEI BspHI BsrBI BssHII BstAPI BstBI BstEII BstZ17I BtgZI ClaI DrdI EagI EarI EciI EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HpaI KpnI MluI MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PfoI PmeI PpiI PshAI PspOMI PspXI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI XmaI ZraI