Restriction map for
pFN21AE0425
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI AspEI AviII AvrII
BalI BamHI BbrPI BbvCI BciVI
BcnI BfrI BlnI BlpI BpmI
Bpu1102I BsaAI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoRI
EcoT22I FseI FspI HapII HindIII
HpaII Ksp632I KspI MfeI MluI
MluNI MroI MscI MspI MunI
MvnI NaeI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SphI Sse8387I SspBI SspI
SwaI Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 633 GGTACC G^GTACC
AcsI 573 GAATTT [GA]^AATT[CT]
ApoI 573 GAATTT [GA]^AATT[CT]
Asp718I 633 GGTACC G^GTACC
AspHI 343 GAGCTC G[AT]GC[AT]^C
AspI 31 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 764 CCCGAG C^[CT]CG[GA]G
BbeI 553 GGCGCC GGCGC^C
BbsI 478 GAAGAC GAAGAC(2/6)
BclI 938 TGATCA T^GATCA
BfaI 1054 CTAG C^TAG
BglII 527 AGATCT A^GATCT
BpuAI 478 GAAGAC GAAGAC(2/6)
BsaBI 933 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 29 GAGACC GGTCTC(1/5)
BsgI 1087 GTGCAG GTGCAG(16/14)
BsiHKAI 343 GAGCTC G[AT]GC[AT]^C
BsmFI 620 GGGAC GGGAC(10/14)
BsmI 75 GAATGC GAATGC(1/-1)
BsoBI 764 CCCGAG C^[CT]CG[GA]G
BspMI 1103 ACCTGC ACCTGC(4/8)
BstEII 247 GGTGACC G^GT[ACGT]ACC
BstPI 247 GGTGACC G^GT[ACGT]ACC
CfoI 554 GCGC GCG^C
DrdI 828 GACATTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 642 CCTCATCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 247 GGTGACC G^GT[ACGT]ACC
FbaI 938 TGATCA T^GATCA
HaeII 553 GGCGCC [GA]GCGC^[CT]
HhaI 554 GCGC GCG^C
HinP1I 554 GCGC G^CGC
HincII 94 GTTAAC GT[CT]^[GA]AC
HindII 94 GTTAAC GT[CT]^[GA]AC
HpaI 94 GTTAAC GTT^AAC
KasI 553 GGCGCC G^GCGCC
KpnI 633 GGTACC GGTAC^C
MaeI 1054 CTAG C^TAG
MaeII 725 ACGT A^CGT
MamI 933 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 553 GGCGCC GG^CGCC
PflFI 31 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 620 GGGACCC [GA]G^G[AT]CC[CT]
SacI 343 GAGCTC GAGCT^C
SfoI 553 GGCGCC GGC^GCC
SpeI 1053 ACTAGT A^CTAGT
StuI 852 AGGCCT AGG^CCT
Tth111I 31 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1028 CCAAAAAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 454 GGCGTC G[GA]^CG[CT]C
553 GGCGCC
AlwI 562 GATCC GGATC(4/5)
267 GGATC
AlwNI 166 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
265 CAGGATCTG
Asp700I 478 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
570 GAAGAATTTC
BcgI 119 GCACAGTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1089 GCAGAGCTTTCG
BglI 596 GCCCTCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1027 GCCAAAAAGGC
BsaHI 454 GGCGTC G[GA]^CG[CT]C
553 GGCGCC
BstYI 267 GGATCT [GA]^GATC[CT]
527 AGATCT
DdeI 524 CTAAG C^T[ACGT]AG
566 CTGAG
DraII 620 GGGACCC [GA]G^G[ACGT]CC[CT]
866 AGGCCCC
DraIII 154 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
776 CACTGGGTG
EcoO109I 620 GGGACCC [GA]G^G[ACGT]CC[CT]
866 AGGCCCC
EcoRV 202 GATATC GAT^ATC
460 GATATC
FauI 624 CCCGC CCCGC(4/6)
981 GCGGG
HgaI 313 GCGTC GACGC(5/10)
455 GCGTC
Hin1I 454 GGCGTC G[GA]^CG[CT]C
553 GGCGCC
MflI 267 GGATCT [GA]^GATC[CT]
527 AGATCT
MslI 711 CACCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
972 CACACCCATG
MspA1I 955 CAGCTG C[AC]G^C[GT]G
1014 CAGCTG
PleI 45 GACTC GAGTC(4/5)
769 GACTC
PstI 255 CTGCAG CTGCA^G
958 CTGCAG
PvuII 955 CAGCTG CAG^CTG
1014 CAGCTG
TfiI 102 GAATC G^A[AT]TC
140 GATTC
XhoII 267 GGATCT [GA]^GATC[CT]
527 AGATCT
XmnI 478 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
570 GAAGAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI AvaII BanI
BanII BbvI BmyI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DpnII
DsaI EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeIII
MboI MboII MnlI MseI MvaI
MwoI NcoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I