(Circular) (Six-base) MAPSORT of: pFN21AE0425.seq Check: 8049 from: 1 to: 5824 pFN21AE0425 5824 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3117 3117 Size: 5824 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3634 278 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3167 1937 Size: 1230 4594 Acc65I G'GTAC_C Cuts at: 2638 3145 2638 Size: 507 5317 AclI AA'CG_TT Cuts at: 1563 3951 4324 1563 Size: 2388 373 3063 Fragments arranged by size: 3063 2388 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2502 3887 5271 5539 1632 Size: 870 1385 1384 268 1917 Fragments arranged by size: 1917 1385 1384 870 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5627 AflIII A'CryG_T Cuts at: 1203 4723 5700 1203 Size: 3520 977 1327 Fragments arranged by size: 3520 1327 977 AgeI A'CCGG_T Cuts at: 4655 4655 Size: 5824 AhdI GACnn_n'nnGTC Cuts at: 4553 4553 Size: 5824 AleI CACnn'nnGTG Cuts at: 1166 2720 1166 Size: 1554 4270 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5824 AlwNI CAG_nnn'CTG Cuts at: 2176 2275 3567 5139 2176 Size: 99 1292 1572 2861 Fragments arranged by size: 2861 1572 1292 99 ApaLI G'TGCA_C Cuts at: 3881 5037 3881 Size: 1156 4668 ApoI r'AATT_y Cuts at: 1493 2578 3133 3359 3511 5620 1493 Size: 1085 555 226 152 2109 1697 Fragments arranged by size: 2109 1697 1085 555 226 152 AseI AT'TA_AT Cuts at: 160 1032 4378 160 Size: 872 3346 1606 Fragments arranged by size: 3346 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 AvaI C'yCGr_G Cuts at: 1870 1943 2769 3149 1870 Size: 73 826 380 4545 Fragments arranged by size: 4545 826 380 73 BamHI G'GATC_C Cuts at: 3154 3521 3154 Size: 367 5457 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2375 2558 2638 3145 4600 Size: 325 217 627 588 183 80 507 1455 Cuts at: 4600 618 Size: 1842 Fragments arranged by size: 1842 1455 627 588 507 325 217 183 80 BanII G_rGCy'C Cuts at: 1391 2141 2352 2820 3143 1391 Size: 750 211 468 323 4072 Fragments arranged by size: 4072 750 468 323 211 BbeI G_GCGC'C Cuts at: 2562 2562 Size: 5824 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2490 928 Size: 971 591 4262 Fragments arranged by size: 4262 971 591 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2113 2147 3083 3117 4015 4049 1903 Size: 34 176 34 936 34 898 34 3678 Fragments arranged by size: 3678 936 898 176 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3716 4926 3716 Size: 1210 4614 BclI T'GATC_A Cuts at: 1548 2943 1548 Size: 1395 4429 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2607 3038 4435 Size: 107 122 71 631 605 935 431 1397 Cuts at: 4435 136 Size: 1525 Fragments arranged by size: 1525 1397 935 631 605 431 122 107 71 BglII A'GATC_T Cuts at: 2532 5819 2532 Size: 3287 2537 BlpI GC'TnA_GC Cuts at: 3241 3241 Size: 5824 Bme1580I G_kGCm'C Cuts at: 1790 3885 5041 1790 Size: 2095 1156 2573 Fragments arranged by size: 2573 2095 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5824 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2792 2905 3555 Size: 416 53 83 399 24 1375 113 650 Cuts at: 3555 4513 442 Size: 958 1753 Fragments arranged by size: 1753 1375 958 650 416 399 113 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5824 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2782 2782 Size: 5824 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4484 1239 Size: 3245 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3949 4814 5112 3949 Size: 865 298 4661 Fragments arranged by size: 4661 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2028 4487 882 Size: 617 529 2459 2219 Fragments arranged by size: 2459 2219 617 529 BsaAI yAC'GTr Cuts at: 493 1204 3615 493 Size: 711 2411 2702 Fragments arranged by size: 2702 2411 711 BsaBI GATnn'nnATC Cuts at: 2942 5818 2942 Size: 2876 2948 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2460 2559 3631 4013 Size: 53 83 186 936 927 99 1072 382 Cuts at: 4013 4648 275 Size: 635 1451 Fragments arranged by size: 1451 1072 936 927 635 382 186 99 83 53 BsaWI w'CCGG_w Cuts at: 4257 4655 4929 5076 5487 4257 Size: 398 274 147 411 4594 Fragments arranged by size: 4594 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5599 Fragments arranged by size: 5599 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5824 BseYI C'CCAG_C Cuts at: 2281 2408 5027 5523 2281 Size: 127 2619 496 2582 Fragments arranged by size: 2619 2582 496 127 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3113 1163 Size: 573 1377 3874 Fragments arranged by size: 3874 1377 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3274 4035 5063 1540 Size: 275 186 1273 761 1028 2301 Fragments arranged by size: 2301 1273 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2352 3885 3970 5041 2352 Size: 1533 85 1071 3135 Fragments arranged by size: 3135 1533 1071 85 BsmI GAATG_Cn' Cuts at: 2086 3335 3428 2086 Size: 1249 93 4482 Fragments arranged by size: 4482 1249 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2141 2352 2820 3143 3885 3970 5041 Size: 399 351 211 468 323 742 85 1071 Cuts at: 5041 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 468 399 351 323 211 85 BspEI T'CCGG_A Cuts at: 5487 5487 Size: 5824 BspHI T'CATG_A Cuts at: 3713 5443 3713 Size: 1730 4094 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3117 3179 844 Size: 2273 62 3489 Fragments arranged by size: 3489 2273 62 BsrBI CCG'CTC Cuts at: 1394 1823 3711 1394 Size: 429 1888 3507 Fragments arranged by size: 3507 1888 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4319 4493 65 Size: 1183 3071 174 1396 Fragments arranged by size: 3071 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4468 4655 1700 Size: 110 2658 187 2869 Fragments arranged by size: 2869 2658 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5824 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5824 BssSI C'ACGA_G Cuts at: 3884 4896 3884 Size: 1012 4812 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5824 BstBI TT'CG_AA Cuts at: 4628 4628 Size: 5824 BstEII G'GTnAC_C Cuts at: 1184 2252 1184 Size: 1068 4756 BstYI r'GATC_y Cuts at: 1972 2272 2532 3154 3521 3906 3923 5375 5461 Size: 300 260 622 367 385 17 1452 86 Cuts at: 5461 5473 5819 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 622 385 367 346 300 260 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2737 3042 3066 513 Size: 552 939 733 305 24 3271 Fragments arranged by size: 3271 939 733 552 305 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5085 Fragments arranged by size: 5085 683 56 BtsI GCAGTG_nn' Cuts at: 2443 3340 4134 4154 2443 Size: 897 794 20 4113 Fragments arranged by size: 4113 897 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5824 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5789 DraI TTT'AAA Cuts at: 3128 3128 Size: 5824 DraIII CAC_nnn'GTG Cuts at: 2164 2786 2164 Size: 622 5202 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2839 4831 809 Size: 507 1523 1992 1802 Fragments arranged by size: 1992 1802 1523 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3271 4160 5789 Size: 54 1425 50 275 18 1441 889 1629 Cuts at: 5789 8 Size: 43 Fragments arranged by size: 1629 1441 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3271 1537 Size: 275 1459 4090 Fragments arranged by size: 4090 1459 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3754 1353 Size: 2401 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2588 4411 4785 4931 387 Size: 1064 138 999 1823 374 146 1280 Fragments arranged by size: 1823 1280 1064 999 374 146 138 EcoICRI GAG'CTC Cuts at: 2350 2350 Size: 5824 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2502 3887 4484 5271 5539 1239 Size: 393 870 1385 597 787 268 1524 Fragments arranged by size: 1524 1385 870 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2651 2651 Size: 5824 EcoO109I rG'GnC_Cy Cuts at: 2626 2872 2626 Size: 246 5578 EcoRI G'AATT_C Cuts at: 3133 3511 3133 Size: 378 5446 EcoRV GAT'ATC Cuts at: 2209 2467 2209 Size: 258 5566 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2368 2599 2368 Size: 231 5593 FspI TGC'GCA Cuts at: 4330 4330 Size: 5824 HaeII r_GCGC'y Cuts at: 1678 2562 4971 1678 Size: 884 2409 2531 Fragments arranged by size: 2531 2409 884 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2025 2057 2178 2210 4465 Size: 32 135 32 453 32 121 32 2255 Cuts at: 4465 4497 4539 4571 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2255 453 135 121 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2101 3168 3414 669 Size: 988 281 163 1067 246 3079 Fragments arranged by size: 3079 1067 988 281 246 163 HindIII A'AGCT_T Cuts at: 748 748 Size: 5824 HpaI GTT'AAC Cuts at: 2101 3414 2101 Size: 1313 4511 KasI G'GCGC_C Cuts at: 2558 2558 Size: 5824 KpnI G_GTAC'C Cuts at: 2642 3149 2642 Size: 507 5317 MfeI C'AATT_G Cuts at: 3423 3423 Size: 5824 MluI A'CGCG_T Cuts at: 5700 5700 Size: 5824 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4938 5122 1305 Size: 3633 184 2007 Fragments arranged by size: 3633 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4002 Fragments arranged by size: 4002 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2720 2981 3782 4141 4300 518 Size: 648 1554 261 801 359 159 2042 Fragments arranged by size: 2042 1554 801 648 359 261 159 MspA1I CmG'CkG Cuts at: 1606 2962 3021 3240 3917 5065 5310 1606 Size: 1356 59 219 677 1148 245 2120 Fragments arranged by size: 2120 1356 1148 677 245 219 59 NarI GG'CG_CC Cuts at: 2559 2559 Size: 5824 NcoI C'CATG_G Cuts at: 513 1065 2004 2737 3042 3066 513 Size: 552 939 733 305 24 3271 Fragments arranged by size: 3271 939 733 552 305 24 NdeI CA'TA_TG Cuts at: 387 387 Size: 5824 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5824 NotI GC'GGCC_GC Cuts at: 3271 3271 Size: 5824 NspI r_CATG'y Cuts at: 1132 1229 3182 4727 1132 Size: 97 1953 1545 2229 Fragments arranged by size: 2229 1953 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5824 PciI A'CATG_T Cuts at: 4723 4723 Size: 5824 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5824 PmeI GTTT'AAAC Cuts at: 3128 3128 Size: 5824 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3893 3925 5406 5438 3893 Size: 32 1481 32 4279 Fragments arranged by size: 4279 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2626 2626 Size: 5824 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5824 PsiI TTA'TAA Cuts at: 3394 3394 Size: 5824 PstI C_TGCA'G Cuts at: 830 2264 2967 3176 830 Size: 1434 703 209 3478 Fragments arranged by size: 3478 1434 703 209 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5824 PvuII CAG'CTG Cuts at: 2962 3021 2962 Size: 59 5765 SacI G_AGCT'C Cuts at: 2352 2352 Size: 5824 SalI G'TCGA_C Cuts at: 1936 3166 1936 Size: 1230 4594 SanDI GG'GwC_CC Cuts at: 2626 2626 Size: 5824 SbfI CC_TGCA'GG Cuts at: 3176 3176 Size: 5824 ScaI AGT'ACT Cuts at: 4072 5784 4072 Size: 1712 4112 SfcI C'TryA_G Cuts at: 826 1045 2260 2963 3016 3172 4307 4988 5179 Size: 219 1215 703 53 156 1135 681 191 Cuts at: 5179 826 Size: 1471 Fragments arranged by size: 1471 1215 1135 703 681 219 191 156 53 SfoI GGC'GCC Cuts at: 2560 2560 Size: 5824 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5824 SmaI CCC'GGG Cuts at: 1872 3151 1872 Size: 1279 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3928 4829 5091 820 Size: 197 926 1985 901 262 1553 Fragments arranged by size: 1985 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3615 493 Size: 3122 2702 SpeI A'CTAG_T Cuts at: 152 3058 152 Size: 2906 2918 SphI G_CATG'C Cuts at: 1132 3182 1132 Size: 2050 3774 SspI AAT'ATT Cuts at: 5 52 3748 5 Size: 47 3696 2081 Fragments arranged by size: 3696 2081 47 StuI AGG'CCT Cuts at: 1517 2859 1517 Size: 1342 4482 StyI C'CwwG_G Cuts at: 513 1065 2004 2621 2737 3042 3066 3263 513 Size: 552 939 617 116 305 24 197 3074 Fragments arranged by size: 3074 939 617 552 305 197 116 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2240 2774 2887 2995 3851 3868 4021 Size: 1255 660 534 113 108 856 17 153 Cuts at: 4021 4206 4651 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 856 660 534 445 185 153 113 108 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4070 5782 96 Size: 274 80 33 51 1443 2093 1712 138 Fragments arranged by size: 2093 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5784 5816 5784 Size: 32 5792 Tth111I GACn'n_nGTC Cuts at: 1316 2039 1316 Size: 723 5101 XbaI T'CTAG_A Cuts at: 3160 3160 Size: 5824 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3040 3040 Size: 5824 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5824 XmaI C'CCGG_G Cuts at: 1870 3149 1870 Size: 1279 4545 XmnI GAAnn'nnTTC Cuts at: 2487 2579 3953 2487 Size: 92 1374 4358 Fragments arranged by size: 4358 1374 92 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3632 276 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI