Restriction map for
pFN21AE0425
(Linear) (Six-base) MAPSORT of: KIEE0425.seq Check: 1757 from: 1 to: 1119
KIEE0425.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1112 1119
Size: 1112 7
Acc65I G'GTAC_C
Cuts at: 0 633 1119
Size: 633 486
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 1119
Size: 497 622
AleI CACnn'nnGTG
Cuts at: 0 715 1119
Size: 715 404
AlwNI CAG_nnn'CTG
Cuts at: 0 171 270 1119
Size: 171 99 849
Fragments arranged by size:
849 171 99
ApoI r'AATT_y
Cuts at: 0 573 1119
Size: 573 546
AvaI C'yCGr_G
Cuts at: 0 764 1119
Size: 764 355
BanI G'GyrC_C
Cuts at: 0 370 553 633 1119
Size: 370 183 80 486
Fragments arranged by size:
486 370 183 80
BanII G_rGCy'C
Cuts at: 0 136 347 815 1119
Size: 136 211 468 304
Fragments arranged by size:
468 304 211 136
BbeI G_GCGC'C
Cuts at: 0 557 1119
Size: 557 562
BbsI GAAGACnn'nnnn_
Cuts at: 0 485 1119
Size: 485 634
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 108 142 1078 1112 1119
Size: 108 34 936 34 7
Fragments arranged by size:
936 108 34 34 7
BclI T'GATC_A
Cuts at: 0 938 1119
Size: 938 181
BglI GCCn_nnn'nGGC
Cuts at: 0 602 1033 1119
Size: 602 431 86
Fragments arranged by size:
602 431 86
BglII A'GATC_T
Cuts at: 0 527 1119
Size: 527 592
BmrI ACTGGGnnnn_n'
Cuts at: 0 787 900 1119
Size: 787 113 219
Fragments arranged by size:
787 219 113
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 777 1119
Size: 777 342
BsaI GGTCTCn'nnnn_
Cuts at: 0 23 1119
Size: 23 1096
BsaBI GATnn'nnATC
Cuts at: 0 937 1119
Size: 937 182
BsaHI Gr'CG_yC
Cuts at: 0 455 554 1119
Size: 455 99 565
Fragments arranged by size:
565 455 99
BseYI C'CCAG_C
Cuts at: 0 276 403 1119
Size: 276 127 716
Fragments arranged by size:
716 276 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1108 1119
Size: 1108 11
BsiHKAI G_wGCw'C
Cuts at: 0 347 1119
Size: 347 772
BsmI GAATG_Cn'
Cuts at: 0 81 1119
Size: 81 1038
Bsp1286I G_dGCh'C
Cuts at: 0 136 347 815 1119
Size: 136 211 468 304
Fragments arranged by size:
468 304 211 136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1112 1119
Size: 1112 7
BstEII G'GTnAC_C
Cuts at: 0 247 1119
Size: 247 872
BstYI r'GATC_y
Cuts at: 0 267 527 1119
Size: 267 260 592
Fragments arranged by size:
592 267 260
BtgI C'CryG_G
Cuts at: 0 732 1037 1061 1119
Size: 732 305 24 58
Fragments arranged by size:
732 305 58 24
BtsI GCAGTG_nn'
Cuts at: 0 438 1119
Size: 438 681
DraIII CAC_nnn'GTG
Cuts at: 0 159 781 1119
Size: 159 622 338
Fragments arranged by size:
622 338 159
DrdI GACnn_nn'nnGTC
Cuts at: 0 834 1119
Size: 834 285
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 583 1119
Size: 583 536
EcoICRI GAG'CTC
Cuts at: 0 345 1119
Size: 345 774
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 1119
Size: 497 622
EcoNI CCTnn'n_nnAGG
Cuts at: 0 646 1119
Size: 646 473
EcoO109I rG'GnC_Cy
Cuts at: 0 621 867 1119
Size: 621 246 252
Fragments arranged by size:
621 252 246
EcoRV GAT'ATC
Cuts at: 0 204 462 1119
Size: 204 258 657
Fragments arranged by size:
657 258 204
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 363 594 1119
Size: 363 231 525
Fragments arranged by size:
525 363 231
HaeII r_GCGC'y
Cuts at: 0 557 1119
Size: 557 562
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 20 52 173 205 1119
Size: 20 32 121 32 914
Fragments arranged by size:
914 121 32 32 20
HincII GTy'rAC
Cuts at: 0 96 1119
Size: 96 1023
HpaI GTT'AAC
Cuts at: 0 96 1119
Size: 96 1023
KasI G'GCGC_C
Cuts at: 0 553 1119
Size: 553 566
KpnI G_GTAC'C
Cuts at: 0 637 1119
Size: 637 482
MslI CAynn'nnrTG
Cuts at: 0 715 976 1119
Size: 715 261 143
Fragments arranged by size:
715 261 143
MspA1I CmG'CkG
Cuts at: 0 957 1016 1119
Size: 957 59 103
Fragments arranged by size:
957 103 59
NarI GG'CG_CC
Cuts at: 0 554 1119
Size: 554 565
NcoI C'CATG_G
Cuts at: 0 732 1037 1061 1119
Size: 732 305 24 58
Fragments arranged by size:
732 305 58 24
PpuMI rG'GwC_Cy
Cuts at: 0 621 1119
Size: 621 498
PstI C_TGCA'G
Cuts at: 0 259 962 1119
Size: 259 703 157
Fragments arranged by size:
703 259 157
PvuII CAG'CTG
Cuts at: 0 957 1016 1119
Size: 957 59 103
Fragments arranged by size:
957 103 59
SacI G_AGCT'C
Cuts at: 0 347 1119
Size: 347 772
SanDI GG'GwC_CC
Cuts at: 0 621 1119
Size: 621 498
SfcI C'TryA_G
Cuts at: 0 255 958 1011 1119
Size: 255 703 53 108
Fragments arranged by size:
703 255 108 53
SfoI GGC'GCC
Cuts at: 0 555 1119
Size: 555 564
SpeI A'CTAG_T
Cuts at: 0 1053 1119
Size: 1053 66
StuI AGG'CCT
Cuts at: 0 854 1119
Size: 854 265
StyI C'CwwG_G
Cuts at: 0 616 732 1037 1061 1119
Size: 616 116 305 24 58
Fragments arranged by size:
616 305 116 58 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 235 769 882 990 1119
Size: 235 534 113 108 129
Fragments arranged by size:
534 235 129 113 108
Tth111I GACn'n_nGTC
Cuts at: 0 34 1119
Size: 34 1085
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1035 1119
Size: 1035 84
XmnI GAAnn'nnTTC
Cuts at: 0 482 574 1119
Size: 482 92 545
Fragments arranged by size:
545 482 92
Enzymes that do cut:
AarI Acc65I AcuI AleI AlwNI ApoI AvaI BanI
BanII BbeI BbsI BcgI BclI BglI BglII BmrI
BplI BsaI BsaBI BsaHI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspMI BstEII BstYI BtgI BtsI DraIII DrdI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII
Hin4I HincII HpaI KasI KpnI MslI MspA1I NarI
NcoI PpuMI PstI PvuII SacI SanDI SfcI SfoI
SpeI StuI StyI TaqII Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BciVI BfrBI BlpI Bme1580I BmgBI
BmtI BpmI Bpu10I BpuEI BsaAI BsaWI BsaXI BseRI
BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI EaeI EagI EarI EcoRI
FseI FspI FspAI HindIII MfeI MluI MmeI MscI
NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SmlI SnaBI SphI SrfI SspI SwaI TatI
TstI XbaI XhoI XmaI ZraI