(Circular) (Six-base) MAPSORT of: pFN21AE2079.seq Check: 7924 from: 1 to: 6961 pFN21AE2079 6961 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4771 278 Size: 53 83 186 936 3235 2468 Fragments arranged by size: 3235 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4304 1937 Size: 2367 4594 Acc65I G'GTAC_C Cuts at: 4282 4282 Size: 6961 AclI AA'CG_TT Cuts at: 1563 5088 5461 1563 Size: 3525 373 3063 Fragments arranged by size: 3525 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2842 3719 5024 6408 6676 1632 Size: 1210 877 1305 1384 268 1917 Fragments arranged by size: 1917 1384 1305 1210 877 268 AfeI AGC'GCT Cuts at: 2750 2750 Size: 6961 AflII C'TTAA_G Cuts at: 820 1017 3890 820 Size: 197 2873 3891 Fragments arranged by size: 3891 2873 197 AflIII A'CryG_T Cuts at: 1203 3405 5860 6837 1203 Size: 2202 2455 977 1327 Fragments arranged by size: 2455 2202 1327 977 AgeI A'CCGG_T Cuts at: 5792 5792 Size: 6961 AhdI GACnn_n'nnGTC Cuts at: 5690 5690 Size: 6961 AleI CACnn'nnGTG Cuts at: 1166 2191 1166 Size: 1025 5936 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2279 3541 1184 Size: 1095 1262 4604 Fragments arranged by size: 4604 1262 1095 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2924 2956 2924 Size: 32 6929 AlwNI CAG_nnn'CTG Cuts at: 2167 2279 3801 4231 4704 6276 2167 Size: 112 1522 430 473 1572 2852 Fragments arranged by size: 2852 1572 1522 473 430 112 ApaI G_GGCC'C Cuts at: 2012 3490 4156 2012 Size: 1478 666 4817 Fragments arranged by size: 4817 1478 666 ApaLI G'TGCA_C Cuts at: 5018 6174 5018 Size: 1156 5805 ApoI r'AATT_y Cuts at: 1493 3599 4246 4270 4496 4648 6757 1493 Size: 2106 647 24 226 152 2109 1697 Fragments arranged by size: 2109 2106 1697 647 226 152 24 AseI AT'TA_AT Cuts at: 160 1032 5515 160 Size: 872 4483 1606 Fragments arranged by size: 4483 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6961 AvaI C'yCGr_G Cuts at: 1870 1943 2095 2943 4286 1870 Size: 73 152 848 1343 4545 Fragments arranged by size: 4545 1343 848 152 73 BamHI G'GATC_C Cuts at: 4291 4658 4291 Size: 367 6594 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2102 4282 5737 618 Size: 325 217 627 315 2180 1455 1842 Fragments arranged by size: 2180 1842 1455 627 325 315 217 BanII G_rGCy'C Cuts at: 1391 2012 2131 2381 2610 2910 3490 4156 4280 Size: 621 119 250 229 300 580 666 124 Cuts at: 4280 1391 Size: 4072 Fragments arranged by size: 4072 666 621 580 300 250 229 124 119 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2553 2717 928 Size: 971 654 164 5172 Fragments arranged by size: 5172 971 654 164 BbvCI CC'TCA_GC Cuts at: 2114 4220 2114 Size: 2106 4855 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2530 2564 5152 5186 1903 Size: 34 593 34 2588 34 3678 Fragments arranged by size: 3678 2588 593 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4853 6063 4853 Size: 1210 5751 BclI T'GATC_A Cuts at: 1548 2961 1548 Size: 1413 5548 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2163 2406 3773 Size: 107 122 71 631 605 491 243 1367 Cuts at: 3773 5572 136 Size: 1799 1525 Fragments arranged by size: 1799 1525 1367 631 605 491 243 122 107 71 BglII A'GATC_T Cuts at: 6956 6956 Size: 6961 BlpI GC'TnA_GC Cuts at: 4378 4378 Size: 6961 Bme1580I G_kGCm'C Cuts at: 1790 2012 2669 3490 4156 5022 6178 1790 Size: 222 657 821 666 866 1156 2573 Fragments arranged by size: 2573 1156 866 821 666 657 222 BmgBI CAC'GTC Cuts at: 1318 2650 1318 Size: 1332 5629 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2486 3018 3580 Size: 416 53 83 399 24 1069 532 562 Cuts at: 3580 4692 5650 442 Size: 1112 958 1753 Fragments arranged by size: 1753 1112 1069 958 562 532 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6961 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2851 3946 2851 Size: 1095 5866 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2614 3283 3689 5621 1239 Size: 1375 669 406 1932 2579 Fragments arranged by size: 2579 1932 1375 669 406 Bpu10I CC'TnA_GC Cuts at: 2114 4220 2114 Size: 2106 4855 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3774 4079 5086 5951 6249 3774 Size: 305 1007 865 298 4486 Fragments arranged by size: 4486 1007 865 305 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2241 3467 3567 3778 5624 882 Size: 617 742 1226 100 211 1846 2219 Fragments arranged by size: 2219 1846 1226 742 617 211 100 BsaAI yAC'GTr Cuts at: 493 1204 4752 493 Size: 711 3548 2702 Fragments arranged by size: 3548 2702 711 BsaBI GATnn'nnATC Cuts at: 6955 6955 Size: 6961 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4768 5150 5785 275 Size: 53 83 186 936 3235 382 635 1451 Fragments arranged by size: 3235 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5394 5792 6066 6213 6624 5394 Size: 398 274 147 411 5731 Fragments arranged by size: 5731 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2558 2588 2924 2954 3533 Size: 30 165 30 1155 30 336 30 579 Cuts at: 3533 3563 4124 4154 1178 Size: 30 561 30 3985 Fragments arranged by size: 3985 1155 579 561 336 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2527 3062 1186 Size: 1341 535 5085 Fragments arranged by size: 5085 1341 535 BseYI C'CCAG_C Cuts at: 2124 2179 2314 4098 6164 6660 2124 Size: 55 135 1784 2066 496 2425 Fragments arranged by size: 2425 2066 1784 496 135 55 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3323 3739 1163 Size: 573 1587 416 4385 Fragments arranged by size: 4385 1587 573 416 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4411 5172 6200 1540 Size: 275 186 2410 761 1028 2301 Fragments arranged by size: 2410 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2553 5022 5107 6178 2553 Size: 2469 85 1071 3336 Fragments arranged by size: 3336 2469 1071 85 BsmI GAATG_Cn' Cuts at: 4472 4565 4472 Size: 93 6868 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2131 2381 2553 2610 2669 2910 Size: 399 222 119 250 172 57 59 241 Cuts at: 2910 3490 4156 4280 5022 5107 6178 1391 Size: 580 666 124 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 666 580 399 250 241 222 172 124 119 85 59 57 BspEI T'CCGG_A Cuts at: 6624 6624 Size: 6961 BspHI T'CATG_A Cuts at: 2995 3048 4850 6580 2995 Size: 53 1802 1730 3376 Fragments arranged by size: 3376 1802 1730 53 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2781 3485 4316 844 Size: 1937 704 831 3489 Fragments arranged by size: 3489 1937 831 704 BsrBI CCG'CTC Cuts at: 1394 1823 2569 4063 4848 1394 Size: 429 746 1494 785 3507 Fragments arranged by size: 3507 1494 785 746 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2344 3523 5456 5630 65 Size: 1183 1096 1179 1933 174 1396 Fragments arranged by size: 1933 1396 1183 1179 1096 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5605 5792 1700 Size: 110 3795 187 2869 Fragments arranged by size: 3795 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6961 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6961 BssSI C'ACGA_G Cuts at: 4179 5021 6033 4179 Size: 842 1012 5107 Fragments arranged by size: 5107 1012 842 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6961 BstBI TT'CG_AA Cuts at: 5765 5765 Size: 6961 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6961 BstXI CCAn_nnnn'nTGG Cuts at: 2687 2753 3241 2687 Size: 66 488 6407 Fragments arranged by size: 6407 488 66 BstYI r'GATC_y Cuts at: 1972 2853 3186 4291 4658 5043 5060 6512 6598 Size: 881 333 1105 367 385 17 1452 86 Cuts at: 6598 6610 6956 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1105 881 385 367 346 333 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2643 2643 Size: 6961 BtgI C'CryG_G Cuts at: 513 1065 2004 2119 2309 2328 513 Size: 552 939 115 190 19 5146 Fragments arranged by size: 5146 939 552 190 115 19 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3549 487 Size: 683 56 2323 3899 Fragments arranged by size: 3899 2323 683 56 BtsI GCAGTG_nn' Cuts at: 2452 2490 4006 4477 5271 5291 2452 Size: 38 1516 471 794 20 4122 Fragments arranged by size: 4122 1516 794 471 38 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6961 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3130 3165 529 Size: 35 2566 35 4325 Fragments arranged by size: 4325 2566 35 35 DraI TTT'AAA Cuts at: 4265 4265 Size: 6961 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3506 5968 809 Size: 507 2190 2462 1802 Fragments arranged by size: 2462 2190 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2449 4408 5297 Size: 54 1425 50 275 18 619 1959 889 Cuts at: 5297 6926 8 Size: 1629 43 Fragments arranged by size: 1959 1629 1425 889 619 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4408 1537 Size: 275 2596 4090 Fragments arranged by size: 4090 2596 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3200 4205 4891 1353 Size: 1847 1005 686 3423 Fragments arranged by size: 3423 1847 1005 686 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5548 5922 6068 387 Size: 1064 138 3959 374 146 1280 Fragments arranged by size: 3959 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2614 2842 3283 3689 3719 5024 5621 Size: 393 982 228 441 406 30 1305 597 Cuts at: 5621 6408 6676 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1305 982 787 597 441 406 393 268 228 30 EcoNI CCTnn'n_nnAGG Cuts at: 4219 4219 Size: 6961 EcoO109I rG'GnC_Cy Cuts at: 2164 2319 3486 3487 3508 4005 4152 4153 4168 Size: 155 1167 1 21 497 147 1 15 Cuts at: 4168 2164 Size: 4957 Fragments arranged by size: 4957 1167 497 155 147 21 15 1 1 EcoRI G'AATT_C Cuts at: 3599 4270 4648 3599 Size: 671 378 5912 Fragments arranged by size: 5912 671 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3620 3620 Size: 6961 FspI TGC'GCA Cuts at: 5467 5467 Size: 6961 HaeII r_GCGC'y Cuts at: 1678 2752 2835 6108 1678 Size: 1074 83 3273 2531 Fragments arranged by size: 3273 2531 1074 83 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3099 3122 3131 3154 3269 Size: 32 135 32 1527 23 9 23 115 Cuts at: 3269 3301 3505 3537 3566 3598 4087 4119 4124 Size: 32 204 32 29 32 489 32 5 Cuts at: 4124 4156 5602 5634 5676 5708 1373 Size: 32 1446 32 42 32 2626 Fragments arranged by size: 2626 1527 1446 489 204 135 115 42 32 32 32 32 32 32 32 32 32 29 23 23 9 5 HincII GTy'rAC Cuts at: 669 1657 1938 3172 4305 4551 669 Size: 988 281 1234 1133 246 3079 Fragments arranged by size: 3079 1234 1133 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6961 HpaI GTT'AAC Cuts at: 4551 4551 Size: 6961 KpnI G_GTAC'C Cuts at: 4286 4286 Size: 6961 MfeI C'AATT_G Cuts at: 4560 4560 Size: 6961 MluI A'CGCG_T Cuts at: 6837 6837 Size: 6961 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2781 3130 3522 6075 6259 1305 Size: 1476 349 392 2553 184 2007 Fragments arranged by size: 2553 2007 1476 392 349 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2451 10 Size: 54 1425 343 619 4520 Fragments arranged by size: 4520 1425 619 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2191 3000 3239 3816 4919 5278 5437 Size: 648 1025 809 239 577 1103 359 159 Cuts at: 5437 518 Size: 2042 Fragments arranged by size: 2042 1103 1025 809 648 577 359 239 159 MspA1I CmG'CkG Cuts at: 1606 2205 2429 3167 4377 5054 6202 6447 1606 Size: 599 224 738 1210 677 1148 245 2120 Fragments arranged by size: 2120 1210 1148 738 677 599 245 224 NcoI C'CATG_G Cuts at: 513 1065 2004 2119 513 Size: 552 939 115 5355 Fragments arranged by size: 5355 939 552 115 NdeI CA'TA_TG Cuts at: 387 3357 387 Size: 2970 3991 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6961 NotI GC'GGCC_GC Cuts at: 4408 4408 Size: 6961 NspI r_CATG'y Cuts at: 1132 1229 2413 2686 3113 3311 3409 3449 4210 Size: 97 1184 273 427 198 98 40 761 Cuts at: 4210 4319 5864 1132 Size: 109 1545 2229 Fragments arranged by size: 2229 1545 1184 761 427 273 198 109 98 97 40 PasI CC'CwG_GG Cuts at: 1348 2161 2662 3629 1348 Size: 813 501 967 4680 Fragments arranged by size: 4680 967 813 501 PciI A'CATG_T Cuts at: 3405 5860 3405 Size: 2455 4506 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6961 PfoI T'CCnGG_A Cuts at: 2778 3666 2778 Size: 888 6073 PmeI GTTT'AAAC Cuts at: 4265 4265 Size: 6961 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2924 2956 5030 5062 6543 6575 2924 Size: 32 2074 32 1481 32 3310 Fragments arranged by size: 3310 2074 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2319 3508 4005 4168 2319 Size: 1189 497 163 5112 Fragments arranged by size: 5112 1189 497 163 PshAI GACnn'nnGTC Cuts at: 1613 3965 1613 Size: 2352 4609 PsiI TTA'TAA Cuts at: 4531 4531 Size: 6961 PspOMI G'GGCC_C Cuts at: 2008 3486 4152 2008 Size: 1478 666 4817 Fragments arranged by size: 4817 1478 666 PstI C_TGCA'G Cuts at: 830 3324 4313 830 Size: 2494 989 3478 Fragments arranged by size: 3478 2494 989 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6961 PvuII CAG'CTG Cuts at: 2429 3167 2429 Size: 738 6223 SalI G'TCGA_C Cuts at: 1936 4303 1936 Size: 2367 4594 SanDI GG'GwC_CC Cuts at: 4005 4005 Size: 6961 SbfI CC_TGCA'GG Cuts at: 4313 4313 Size: 6961 ScaI AGT'ACT Cuts at: 5209 6921 5209 Size: 1712 5249 SexAI A'CCwGG_T Cuts at: 3342 3342 Size: 6961 SfcI C'TryA_G Cuts at: 826 1045 3320 3802 4309 5444 6125 6316 826 Size: 219 2275 482 507 1135 681 191 1471 Fragments arranged by size: 2275 1471 1135 681 507 482 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6961 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6961 SmaI CCC'GGG Cuts at: 1872 2945 4288 1872 Size: 1073 1343 4545 Fragments arranged by size: 4545 1343 1073 SmlI C'TyrA_G Cuts at: 820 1017 1943 3789 3890 4058 5065 5966 6228 Size: 197 926 1846 101 168 1007 901 262 Cuts at: 6228 820 Size: 1553 Fragments arranged by size: 1846 1553 1007 926 901 262 197 168 101 SnaBI TAC'GTA Cuts at: 493 4752 493 Size: 4259 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6961 SphI G_CATG'C Cuts at: 1132 3311 4210 4319 1132 Size: 2179 899 109 3774 Fragments arranged by size: 3774 2179 899 109 SspI AAT'ATT Cuts at: 5 52 4885 5 Size: 47 4833 2081 Fragments arranged by size: 4833 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6961 StyI C'CwwG_G Cuts at: 513 1065 2004 2119 4120 4400 513 Size: 552 939 115 2001 280 3074 Fragments arranged by size: 3074 2001 939 552 280 115 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2321 2720 3137 3743 4988 5005 5158 Size: 1255 741 399 417 606 1245 17 153 Cuts at: 5158 5343 5788 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1245 741 606 445 417 399 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5207 6919 96 Size: 274 80 33 51 1443 3230 1712 138 Fragments arranged by size: 3230 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2558 2590 3901 3933 6921 6953 2558 Size: 32 1311 32 2988 32 2566 Fragments arranged by size: 2988 2566 1311 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2519 1316 Size: 1203 5758 XbaI T'CTAG_A Cuts at: 4297 4297 Size: 6961 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3394 3394 Size: 6961 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6961 XmaI C'CCGG_G Cuts at: 1870 2943 4286 1870 Size: 1073 1343 4545 Fragments arranged by size: 4545 1343 1073 XmnI GAAnn'nnTTC Cuts at: 5090 5090 Size: 6961 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4769 276 Size: 53 83 186 936 3235 2468 Fragments arranged by size: 3235 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI