################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII AccI AccII AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BsiEI BsiWI BsoBI Bsp1407I BspDI BspEI BspHI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRV EcoT22I FauI FseI FspI HincII HindII HindIII HpaI KasI KspI MamI MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SbfI SfcI SfiI SfoI SfuI SgrAI SmaI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 1843 GGTACC G^GTACC AcyI 37 GGCGTC G[GA]^CG[CT]C AlwI 1758 GGATC GGATC(4/5) AlwNI 698 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp718I 1843 GGTACC G^GTACC BanI 1843 GGTACC G^G[CT][GA]CC BanII 560 GAGCTC G[GA]GC[CT]^C BciVI 504 GGATAC GTATCC(6/5) BclI 354 TGATCA T^GATCA BsaAI 734 TACGTA [CT]AC^GT[GA] BsaHI 37 GGCGTC G[GA]^CG[CT]C BsmI 294 GAATGC GAATGC(1/-1) BsrBI 2070 GAGCGG CCGCTC(-3/-3) BsrDI 2081 CATTGC GCAATG(2/0) BstAPI 1342 GCAGCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 1758 GGATCT [GA]^GATC[CT] CfoI 1164 GCGC GCG^C DraII 1809 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoNI 1791 CCTTAGTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1809 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoRI 612 GAATTC G^AATTC FbaI 354 TGATCA T^GATCA HaeII 1163 GGCGCT [GA]GCGC^[CT] HgaI 38 GCGTC GACGC(5/10) HhaI 1164 GCGC GCG^C Hin1I 37 GGCGTC G[GA]^CG[CT]C HinP1I 1164 GCGC G^CGC KpnI 1843 GGTACC GGTAC^C MflI 1758 GGATCT [GA]^GATC[CT] NcoI 1994 CCATGG C^CATGG NdeI 1026 CATATG CA^TATG PpuMI 1809 AGGTCCT [GA]G^G[AT]CC[CT] SacI 560 GAGCTC GAGCT^C SapI 3 GCTCTTC GCTCTTC(1/4) ScaI 1282 AGTACT AGT^ACT SexAI 508 ACCAGGT A^CC[AT]GGT SfaNI 772 GCATC GCATC(5/9) SnaBI 734 TACGTA TAC^GTA XbaI 1639 TCTAGA T^CTAGA XcmI 24 CCAGCCCCAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1758 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcsI 367 AAATTT [GA]^AATT[CT] 612 GAATTC AflIII 192 ACATGT A^C[GA][CT]GT 887 ACATGT ApoI 367 AAATTT [GA]^AATT[CT] 612 GAATTC BalI 1734 TGGCCA TGG^CCA 1991 TGGCCA BcnI 1427 CCCGG CC^[GC]GG 2091 CCCGG BsgI 2127 CTGCAC GTGCAG(16/14) 99 GTGCAG BsmBI 1464 CGTCTC CGTCTC(1/5) 1865 CGTCTC BspLU11I 192 ACATGT A^CATGT 887 ACATGT BspMI 1551 ACCTGC ACCTGC(4/8) 349 GCAGGT Cac8I 23 GCCAGC GC[ACGT]^[ACGT]GC 1627 GCTTGC DsaI 1211 CCACGG C^C[GA][CT]GG 1994 CCATGG EaeI 1734 TGGCCA [CT]^GGCC[GA] 1991 TGGCCA EcoT14I 1421 CCAAGG C^C[AT][AT]GG 1994 CCATGG HapII 1428 CCGG C^CGG 2092 CCGG HpaII 1428 CCGG C^CGG 2092 CCGG MluNI 1734 TGGCCA TGG^CCA 1991 TGGCCA MscI 1734 TGGCCA TGG^CCA 1991 TGGCCA MspI 1428 CCGG C^CGG 2092 CCGG NciI 1427 CCCGG CC^[GC]GG 2091 CCCGG NlaIV 318 GGTTCC GG[ACGT]^[ACGT]CC 1843 GGTACC PflMI 365 CCAAATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1040 CCACACCCTGG StyI 1421 CCAAGG C^C[AT][AT]GG 1994 CCATGG Van91I 365 CCAAATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1040 CCACACCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AciI AfaI AluI Asp700I AspHI AvaII BbsI BbvI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstEII BstF5I BstNI BstPI Cfr13I DdeI DpnII EarI EcoO65I EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII NspI PleI RsaI Sau3AI Sau96I ScrFI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmnI