Restriction map for
pFN21AE2071
(Circular) (Six-base) MAPSORT of: pFN21AE2071.seq Check: 9859 from: 1 to: 6895
pFN21AE2071 6895 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2345 3565 2345
Size: 1220 5675
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4705 278
Size: 53 83 186 936 3169 2468
Fragments arranged by size:
3169 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4238 1937
Size: 2301 4594
Acc65I G'GTAC_C
Cuts at: 3848 4216 3848
Size: 368 6527
AclI AA'CG_TT
Cuts at: 1563 5022 5395 1563
Size: 3459 373 3063
Fragments arranged by size:
3459 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3876 4958 6342 6610 1632
Size: 2244 1082 1384 268 1917
Fragments arranged by size:
2244 1917 1384 1082 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6698
AflIII A'CryG_T
Cuts at: 1203 2197 2892 5794 6771 1203
Size: 994 695 2902 977 1327
Fragments arranged by size:
2902 1327 994 977 695
AgeI A'CCGG_T
Cuts at: 5726 5726
Size: 6895
AhdI GACnn_n'nnGTC
Cuts at: 5624 5624
Size: 6895
AleI CACnn'nnGTG
Cuts at: 1166 3098 1166
Size: 1932 4963
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3649 1184
Size: 2465 4430
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2136 2168 2136
Size: 32 6863
AlwNI CAG_nnn'CTG
Cuts at: 2708 4638 6210 2708
Size: 1930 1572 3393
Fragments arranged by size:
3393 1930 1572
ApaLI G'TGCA_C
Cuts at: 4952 6108 4952
Size: 1156 5739
ApoI r'AATT_y
Cuts at: 1493 2372 2617 4204 4430 4582 6691 1493
Size: 879 245 1587 226 152 2109 1697
Fragments arranged by size:
2109 1697 1587 879 245 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5449 160
Size: 872 4417 1606
Fragments arranged by size:
4417 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6895
AvaI C'yCGr_G
Cuts at: 1870 1943 4220 1870
Size: 73 2277 4545
Fragments arranged by size:
4545 2277 73
BamHI G'GATC_C
Cuts at: 4225 4592 4225
Size: 367 6528
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3848 4216 5671 618
Size: 325 217 627 2061 368 1455 1842
Fragments arranged by size:
2061 1842 1455 627 368 325 217
BanII G_rGCy'C
Cuts at: 1391 2569 4214 1391
Size: 1178 1645 4072
Fragments arranged by size:
4072 1645 1178
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2055 2921 3798 928
Size: 971 156 866 877 4025
Fragments arranged by size:
4025 971 877 866 156
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5086 5120 1903
Size: 34 50 34 3065 34 3678
Fragments arranged by size:
3678 3065 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2503 4787 5997 2503
Size: 2284 1210 3401
Fragments arranged by size:
3401 2284 1210
BclI T'GATC_A
Cuts at: 1548 2359 1548
Size: 811 6084
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5506 136
Size: 107 122 71 631 605 3834 1525
Fragments arranged by size:
3834 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6890 6890
Size: 6895
BlpI GC'TnA_GC
Cuts at: 4312 4312
Size: 6895
Bme1580I G_kGCm'C
Cuts at: 1790 3358 4956 6112 1790
Size: 1568 1598 1156 2573
Fragments arranged by size:
2573 1598 1568 1156
BmgBI CAC'GTC
Cuts at: 1318 3096 1318
Size: 1778 5117
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2144 2916 3523
Size: 416 53 83 399 24 727 772 607
Cuts at: 3523 4626 5584 442
Size: 1103 958 1753
Fragments arranged by size:
1753 1103 958 772 727 607 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6895
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3652 3652
Size: 6895
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2907 3390 3704 5555 1239
Size: 1668 483 314 1851 2579
Fragments arranged by size:
2579 1851 1668 483 314
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2417 2995 3069 3658 5020 5885 6183 2417
Size: 578 74 589 1362 865 298 3129
Fragments arranged by size:
3129 1362 865 589 578 298 74
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3046 3366 3441 3560 3919 5558 882
Size: 617 1547 320 75 119 359 1639 2219
Fragments arranged by size:
2219 1639 1547 617 359 320 119 75
BsaAI yAC'GTr
Cuts at: 493 1204 2741 4686 493
Size: 711 1537 1945 2702
Fragments arranged by size:
2702 1945 1537 711
BsaBI GATnn'nnATC
Cuts at: 6889 6889
Size: 6895
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2043 4702 5084 5719
Size: 53 83 186 936 510 2659 382 635
Cuts at: 5719 275
Size: 1451
Fragments arranged by size:
2659 1451 936 635 510 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5328 5726 6000 6147 6558 5328
Size: 398 274 147 411 5665
Fragments arranged by size:
5665 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2136 2166 1178
Size: 30 165 30 733 30 5907
Fragments arranged by size:
5907 733 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3203 3454 3676 1186
Size: 2017 251 222 4405
Fragments arranged by size:
4405 2017 251 222
BseYI C'CCAG_C
Cuts at: 2034 4112 6098 6594 2034
Size: 2078 1986 496 2335
Fragments arranged by size:
2335 2078 1986 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2125 4117 1163
Size: 573 389 1992 3941
Fragments arranged by size:
3941 1992 573 389
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4345 5106 6134 1540
Size: 275 186 2344 761 1028 2301
Fragments arranged by size:
2344 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2025 2569 3072 4956 5041 6112 2025
Size: 544 503 1884 85 1071 2808
Fragments arranged by size:
2808 1884 1071 544 503 85
BsmI GAATG_Cn'
Cuts at: 2305 4406 4499 2305
Size: 2101 93 4701
Fragments arranged by size:
4701 2101 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3475 3876 3475
Size: 401 6494
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2569 3072 3358 4214 4956 5041
Size: 399 235 544 503 286 856 742 85
Cuts at: 5041 6112 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 856 742 544 503 399 286
235 85
BspEI T'CCGG_A
Cuts at: 6558 6558
Size: 6895
BspHI T'CATG_A
Cuts at: 4784 6514 4784
Size: 1730 5165
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2345 3565 4250 844
Size: 1501 1220 685 3489
Fragments arranged by size:
3489 1501 1220 685
BsrBI CCG'CTC
Cuts at: 1394 1823 4077 4782 1394
Size: 429 2254 705 3507
Fragments arranged by size:
3507 2254 705 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4085 5390 5564 65
Size: 1183 2837 1305 174 1396
Fragments arranged by size:
2837 1396 1305 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5539 5726 1700
Size: 110 3729 187 2869
Fragments arranged by size:
3729 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6895
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6895
BssSI C'ACGA_G
Cuts at: 4955 5967 4955
Size: 1012 5883
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3353 794
Size: 2559 4336
BstBI TT'CG_AA
Cuts at: 5699 5699
Size: 6895
BstEII G'GTnAC_C
Cuts at: 1184 3517 3782 4099 1184
Size: 2333 265 317 3980
Fragments arranged by size:
3980 2333 317 265
BstYI r'GATC_y
Cuts at: 1972 3763 4225 4592 4977 4994 6446 6532 6544
Size: 1791 462 367 385 17 1452 86 12
Cuts at: 6544 6890 1972
Size: 346 1977
Fragments arranged by size:
1977 1791 1452 462 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3216 3999 513
Size: 552 2151 783 3409
Fragments arranged by size:
3409 2151 783 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6156
Fragments arranged by size:
6156 683 56
BtsI GCAGTG_nn'
Cuts at: 2113 4411 5205 5225 2113
Size: 2298 794 20 3783
Fragments arranged by size:
3783 2298 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6895
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6860
DraI TTT'AAA
Cuts at: 4199 4199
Size: 6895
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5902 809
Size: 507 4586 1802
Fragments arranged by size:
4586 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3739 3996 4342
Size: 54 1425 50 275 18 1909 257 346
Cuts at: 4342 5231 6860 8
Size: 889 1629 43
Fragments arranged by size:
1909 1629 1425 889 346 275 257 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4342 1537
Size: 275 2530 4090
Fragments arranged by size:
4090 2530 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2015 2082 2182 2479 3387 3445 3704 3921
Size: 662 67 100 297 908 58 259 217
Cuts at: 3921 4825 1353
Size: 904 3423
Fragments arranged by size:
3423 908 904 662 297 259 217 100
67 58
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2015 5482 5856 6002 387
Size: 1064 138 426 3467 374 146 1280
Fragments arranged by size:
3467 1280 1064 426 374 146 138
EcoICRI GAG'CTC
Cuts at: 2567 2567
Size: 6895
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2907 3390 3704 3876 4958 5555 6342
Size: 393 1275 483 314 172 1082 597 787
Cuts at: 6342 6610 1239
Size: 268 1524
Fragments arranged by size:
1524 1275 1082 787 597 483 393 314
268 172
EcoNI CCTnn'n_nnAGG
Cuts at: 3800 3800
Size: 6895
EcoO109I rG'GnC_Cy
Cuts at: 3815 3815
Size: 6895
EcoRI G'AATT_C
Cuts at: 2617 4204 4582 2617
Size: 1587 378 4930
Fragments arranged by size:
4930 1587 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2290 2290
Size: 6895
FspI TGC'GCA
Cuts at: 5401 5401
Size: 6895
HaeII r_GCGC'y
Cuts at: 1678 3172 6042 1678
Size: 1494 2870 2531
Fragments arranged by size:
2870 2531 1494
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2083 2115 3080 3112 3444
Size: 32 135 32 511 32 965 32 332
Cuts at: 3444 3476 3565 3597 3854 3886 4013 4045 5536
Size: 32 89 32 257 32 127 32 1491
Cuts at: 5536 5568 5610 5642 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1491 965 511 332 257 135 127
89 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4239 4485 669
Size: 988 281 2301 246 3079
Fragments arranged by size:
3079 2301 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6895
HpaI GTT'AAC
Cuts at: 4485 4485
Size: 6895
KpnI G_GTAC'C
Cuts at: 3852 4220 3852
Size: 368 6527
MfeI C'AATT_G
Cuts at: 4494 4494
Size: 6895
MluI A'CGCG_T
Cuts at: 6771 6771
Size: 6895
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6009 6193 1305
Size: 4704 184 2007
Fragments arranged by size:
4704 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3741 3998 10
Size: 54 1425 343 1909 257 2907
Fragments arranged by size:
2907 1909 1425 343 257 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2298 2891 3098 4853 5212 5371 518
Size: 648 1132 593 207 1755 359 159 2042
Fragments arranged by size:
2042 1755 1132 648 593 359 207 159
MspA1I CmG'CkG
Cuts at: 1606 4311 4988 6136 6381 1606
Size: 2705 677 1148 245 2120
Fragments arranged by size:
2705 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3999 513
Size: 552 2934 3409
Fragments arranged by size:
3409 2934 552
NdeI CA'TA_TG
Cuts at: 387 3032 387
Size: 2645 4250
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6895
NotI GC'GGCC_GC
Cuts at: 4342 4342
Size: 6895
NspI r_CATG'y
Cuts at: 1132 1229 2201 2412 2896 4253 5798 1132
Size: 97 972 211 484 1357 1545 2229
Fragments arranged by size:
2229 1545 1357 972 484 211 97
PasI CC'CwG_GG
Cuts at: 1348 3306 1348
Size: 1958 4937
PciI A'CATG_T
Cuts at: 2197 2892 5794 2197
Size: 695 2902 3298
Fragments arranged by size:
3298 2902 695
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2376 3051 1108
Size: 1268 675 4952
Fragments arranged by size:
4952 1268 675
PfoI T'CCnGG_A
Cuts at: 4064 4064
Size: 6895
PmeI GTTT'AAAC
Cuts at: 4199 4199
Size: 6895
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2136 2168 3898 3930 4964 4996 6477 6509 2136
Size: 32 1730 32 1034 32 1481 32 2522
Fragments arranged by size:
2522 1730 1481 1034 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3815 3815
Size: 6895
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6895
PsiI TTA'TAA
Cuts at: 4465 4465
Size: 6895
PstI C_TGCA'G
Cuts at: 830 4247 830
Size: 3417 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6895
SacI G_AGCT'C
Cuts at: 2569 2569
Size: 6895
SalI G'TCGA_C
Cuts at: 1936 4237 1936
Size: 2301 4594
SapI GCTCTTCn'nnn_
Cuts at: 2015 2015
Size: 6895
SbfI CC_TGCA'GG
Cuts at: 4247 4247
Size: 6895
ScaI AGT'ACT
Cuts at: 3289 5143 6855 3289
Size: 1854 1712 3329
Fragments arranged by size:
3329 1854 1712
SexAI A'CCwGG_T
Cuts at: 2513 2513
Size: 6895
SfcI C'TryA_G
Cuts at: 826 1045 4243 5378 6059 6250 826
Size: 219 3198 1135 681 191 1471
Fragments arranged by size:
3198 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6895
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6895
SmaI CCC'GGG
Cuts at: 1872 4222 1872
Size: 2350 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2432 3010 3084 3673 4999 5900
Size: 197 926 489 578 74 589 1326 901
Cuts at: 5900 6162 820
Size: 262 1553
Fragments arranged by size:
1553 1326 926 901 589 578 489 262
197 74
SnaBI TAC'GTA
Cuts at: 493 2741 4686 493
Size: 2248 1945 2702
Fragments arranged by size:
2702 2248 1945
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6895
SphI G_CATG'C
Cuts at: 1132 4253 1132
Size: 3121 3774
SspI AAT'ATT
Cuts at: 5 52 4819 5
Size: 47 4767 2081
Fragments arranged by size:
4767 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6895
StyI C'CwwG_G
Cuts at: 513 1065 3426 3999 4334 513
Size: 552 2361 573 335 3074
Fragments arranged by size:
3074 2361 573 552 335
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3505 3559 4072 4922 4939 5092 5277
Size: 1255 1925 54 513 850 17 153 185
Cuts at: 5277 5722 325
Size: 445 1498
Fragments arranged by size:
1925 1498 1255 850 513 445 185 153
54 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2200 3287 5141
Size: 274 80 33 51 1443 223 1087 1854
Cuts at: 5141 6853 96
Size: 1712 138
Fragments arranged by size:
1854 1712 1443 1087 274 223 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2501 2533 6855 6887 2501
Size: 32 4322 32 2509
Fragments arranged by size:
4322 2509 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6895
XbaI T'CTAG_A
Cuts at: 3644 4231 3644
Size: 587 6308
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2036 2036
Size: 6895
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6895
XmaI C'CCGG_G
Cuts at: 1870 4220 1870
Size: 2350 4545
XmnI GAAnn'nnTTC
Cuts at: 2273 3529 3753 5024 2273
Size: 1256 224 1271 4144
Fragments arranged by size:
4144 1271 1256 224
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4703 276
Size: 53 83 186 936 3169 2468
Fragments arranged by size:
3169 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SacI SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI
SfiI SfoI SrfI SwaI