(Linear) (Six-base) MAPSORT of: KIEE2071.seq Check: 8122 from: 1 to: 2190 KIEE2071.orf With 182 enzymes: SgfI * July 15, 2010 18:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 340 1560 2190 Size: 340 1220 630 Fragments arranged by size: 1220 630 340 Acc65I G'GTAC_C Cuts at: 0 1843 2190 Size: 1843 347 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1871 2190 Size: 1871 319 AflIII A'CryG_T Cuts at: 0 192 887 2190 Size: 192 695 1303 Fragments arranged by size: 1303 695 192 AleI CACnn'nnGTG Cuts at: 0 1093 2190 Size: 1093 1097 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1644 2190 Size: 1644 546 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 131 163 2190 Size: 131 32 2027 Fragments arranged by size: 2027 131 32 AlwNI CAG_nnn'CTG Cuts at: 0 703 2190 Size: 703 1487 ApoI r'AATT_y Cuts at: 0 367 612 2190 Size: 367 245 1578 Fragments arranged by size: 1578 367 245 BanI G'GyrC_C Cuts at: 0 1843 2190 Size: 1843 347 BanII G_rGCy'C Cuts at: 0 564 2190 Size: 564 1626 BbsI GAAGACnn'nnnn_ Cuts at: 0 50 916 1793 2190 Size: 50 866 877 397 Fragments arranged by size: 877 866 397 50 BciVI GTATCCnnnnn_n' Cuts at: 0 498 2190 Size: 498 1692 BclI T'GATC_A Cuts at: 0 354 2190 Size: 354 1836 Bme1580I G_kGCm'C Cuts at: 0 1353 2190 Size: 1353 837 BmgBI CAC'GTC Cuts at: 0 1091 2190 Size: 1091 1099 BmrI ACTGGGnnnn_n' Cuts at: 0 139 911 1518 2190 Size: 139 772 607 672 Fragments arranged by size: 772 672 607 139 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1647 2190 Size: 1647 543 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 902 1385 1699 2190 Size: 902 483 314 491 Fragments arranged by size: 902 491 483 314 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 412 990 1064 1653 2190 Size: 412 578 74 589 537 Fragments arranged by size: 589 578 537 412 74 BsaI GGTCTCn'nnnn_ Cuts at: 0 1041 1361 1436 1555 1914 2190 Size: 1041 320 75 119 359 276 Fragments arranged by size: 1041 359 320 276 119 75 BsaAI yAC'GTr Cuts at: 0 736 2190 Size: 736 1454 BsaHI Gr'CG_yC Cuts at: 0 38 2190 Size: 38 2152 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 131 161 2190 Size: 131 30 2029 Fragments arranged by size: 2029 131 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1198 1449 1671 2190 Size: 1198 251 222 519 Fragments arranged by size: 1198 519 251 222 BseYI C'CCAG_C Cuts at: 0 29 2107 2190 Size: 29 2078 83 Fragments arranged by size: 2078 83 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 120 2112 2190 Size: 120 1992 78 Fragments arranged by size: 1992 120 78 BsiHKAI G_wGCw'C Cuts at: 0 20 564 1067 2190 Size: 20 544 503 1123 Fragments arranged by size: 1123 544 503 20 BsmI GAATG_Cn' Cuts at: 0 300 2190 Size: 300 1890 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1470 1871 2190 Size: 1470 401 319 Fragments arranged by size: 1470 401 319 Bsp1286I G_dGCh'C Cuts at: 0 20 564 1067 1353 2190 Size: 20 544 503 286 837 Fragments arranged by size: 837 544 503 286 20 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 340 1560 2190 Size: 340 1220 630 Fragments arranged by size: 1220 630 340 BsrBI CCG'CTC Cuts at: 0 2072 2190 Size: 2072 118 BsrDI GCAATG_nn' Cuts at: 0 2080 2190 Size: 2080 110 BstAPI GCAn_nnn'nTGC Cuts at: 0 1348 2190 Size: 1348 842 BstEII G'GTnAC_C Cuts at: 0 1512 1777 2094 2190 Size: 1512 265 317 96 Fragments arranged by size: 1512 317 265 96 BstYI r'GATC_y Cuts at: 0 1758 2190 Size: 1758 432 BtgI C'CryG_G Cuts at: 0 1211 1994 2190 Size: 1211 783 196 Fragments arranged by size: 1211 783 196 BtsI GCAGTG_nn' Cuts at: 0 108 2190 Size: 108 2082 EaeI y'GGCC_r Cuts at: 0 1734 1991 2190 Size: 1734 257 199 Fragments arranged by size: 1734 257 199 EarI CTCTTCn'nnn_ Cuts at: 0 10 77 177 474 1382 1440 1699 1916 Size: 10 67 100 297 908 58 259 217 Cuts at: 1916 2190 Size: 274 Fragments arranged by size: 908 297 274 259 217 100 67 58 10 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 10 2190 Size: 10 2180 EcoICRI GAG'CTC Cuts at: 0 562 2190 Size: 562 1628 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 902 1385 1699 1871 2190 Size: 902 483 314 172 319 Fragments arranged by size: 902 483 319 314 172 EcoNI CCTnn'n_nnAGG Cuts at: 0 1795 2190 Size: 1795 395 EcoO109I rG'GnC_Cy Cuts at: 0 1810 2190 Size: 1810 380 EcoRI G'AATT_C Cuts at: 0 612 2190 Size: 612 1578 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 285 2190 Size: 285 1905 HaeII r_GCGC'y Cuts at: 0 1167 2190 Size: 1167 1023 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 78 110 1075 1107 1439 1471 1560 1592 Size: 78 32 965 32 332 32 89 32 Cuts at: 1592 1849 1881 2008 2040 2190 Size: 257 32 127 32 150 Fragments arranged by size: 965 332 257 150 127 89 78 32 32 32 32 32 32 KpnI G_GTAC'C Cuts at: 0 1847 2190 Size: 1847 343 MscI TGG'CCA Cuts at: 0 1736 1993 2190 Size: 1736 257 197 Fragments arranged by size: 1736 257 197 MslI CAynn'nnrTG Cuts at: 0 293 886 1093 2190 Size: 293 593 207 1097 Fragments arranged by size: 1097 593 293 207 NcoI C'CATG_G Cuts at: 0 1994 2190 Size: 1994 196 NdeI CA'TA_TG Cuts at: 0 1027 2190 Size: 1027 1163 NspI r_CATG'y Cuts at: 0 196 407 891 2190 Size: 196 211 484 1299 Fragments arranged by size: 1299 484 211 196 PasI CC'CwG_GG Cuts at: 0 1301 2190 Size: 1301 889 PciI A'CATG_T Cuts at: 0 192 887 2190 Size: 192 695 1303 Fragments arranged by size: 1303 695 192 PflMI CCAn_nnn'nTGG Cuts at: 0 371 1046 2190 Size: 371 675 1144 Fragments arranged by size: 1144 675 371 PfoI T'CCnGG_A Cuts at: 0 2059 2190 Size: 2059 131 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 131 163 1893 1925 2190 Size: 131 32 1730 32 265 Fragments arranged by size: 1730 265 131 32 32 PpuMI rG'GwC_Cy Cuts at: 0 1810 2190 Size: 1810 380 SacI G_AGCT'C Cuts at: 0 564 2190 Size: 564 1626 SapI GCTCTTCn'nnn_ Cuts at: 0 10 2190 Size: 10 2180 ScaI AGT'ACT Cuts at: 0 1284 2190 Size: 1284 906 SexAI A'CCwGG_T Cuts at: 0 508 2190 Size: 508 1682 SmlI C'TyrA_G Cuts at: 0 427 1005 1079 1668 2190 Size: 427 578 74 589 522 Fragments arranged by size: 589 578 522 427 74 SnaBI TAC'GTA Cuts at: 0 736 2190 Size: 736 1454 StyI C'CwwG_G Cuts at: 0 1421 1994 2190 Size: 1421 573 196 Fragments arranged by size: 1421 573 196 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1500 1554 2067 2190 Size: 1500 54 513 123 Fragments arranged by size: 1500 513 123 54 TatI w'GTAC_w Cuts at: 0 195 1282 2190 Size: 195 1087 908 Fragments arranged by size: 1087 908 195 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 496 528 2190 Size: 496 32 1662 Fragments arranged by size: 1662 496 32 XbaI T'CTAG_A Cuts at: 0 1639 2190 Size: 1639 551 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 31 2190 Size: 31 2159 XmnI GAAnn'nnTTC Cuts at: 0 268 1524 1748 2190 Size: 268 1256 224 442 Fragments arranged by size: 1256 442 268 224 Enzymes that do cut: AarI Acc65I AcuI AflIII AleI AlfI AloI AlwNI ApoI BanI BanII BbsI BciVI BclI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BstAPI BstEII BstYI BtgI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I KpnI MscI MslI NcoI NdeI NspI PasI PciI PflMI PfoI PpiI PpuMI SacI SapI ScaI SexAI SmlI SnaBI StyI TaqII TatI TstI XbaI XcmI XmnI Enzymes that do not cut: AatII AccI AclI AfeI AflII AgeI AhdI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BfrBI BglI BglII BlpI BmtI Bpu10I BsaBI BsaWI BsiEI BsiWI BspEI BspHI BsrFI BsrGI BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoRV FseI FspI FspAI HincII HindIII HpaI KasI MfeI MluI MmeI MspA1I NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SanDI SbfI SfcI SfiI SfoI SgfI SgrAI SmaI SpeI SphI SrfI SspI StuI SwaI Tth111I XhoI XmaI ZraI