Restriction map for
pFN21AE0420
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BlnI
BlpI Bpu1102I BsaBI BsaI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspLU11I
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII Bst1107I BstAPI BstBI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI HincII HindII
HindIII HpaI KspI MamI MfeI
MluI MluNI MscI MseI MunI
NaeI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tth111I Van91I
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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Acc65I 77 GGTACC G^GTACC
AccII 375 CGCG CG^CG
AccIII 455 TCCGGA T^CCGGA
AcyI 235 GGCGCC G[GA]^CG[CT]C
Asp700I 270 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 77 GGTACC G^GTACC
BbeI 235 GGCGCC GGCGC^C
BbrPI 574 CACGTG CAC^GTG
BbsI 292 GAAGAC GAAGAC(2/6)
BglII 285 AGATCT A^GATCT
BpuAI 292 GAAGAC GAAGAC(2/6)
BsaAI 574 CACGTG [CT]AC^GT[GA]
BsaHI 235 GGCGCC G[GA]^CG[CT]C
BsaWI 455 TCCGGA [AT]^CCGG[AT]
BseAI 455 TCCGGA T^CCGGA
BseRI 100 GAGGAG GAGGAG(10/8)
BsgI 162 GTGCAG GTGCAG(16/14)
BspEI 455 TCCGGA T^CCGGA
BspHI 320 TCATGA T^CATGA
BspMI 483 GCAGGT ACCTGC(4/8)
BssSI 82 CACGAG CACGAG(-5/-1)
BstEII 513 GGTGACC G^GT[ACGT]ACC
BstPI 513 GGTGACC G^GT[ACGT]ACC
BstUI 375 CGCG CG^CG
BstXI 569 CCAGCCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 285 AGATCT [GA]^GATC[CT]
CfoI 236 GCGC GCG^C
DraII 350 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoNI 138 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 350 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 513 GGTGACC G^GT[ACGT]ACC
FauI 373 CCCGC CCCGC(4/6)
HaeII 235 GGCGCC [GA]GCGC^[CT]
HapII 456 CCGG C^CGG
HhaI 236 GCGC GCG^C
Hin1I 235 GGCGCC G[GA]^CG[CT]C
HinP1I 236 GCGC G^CGC
HpaII 456 CCGG C^CGG
KasI 235 GGCGCC G^GCGCC
KpnI 77 GGTACC GGTAC^C
MaeII 575 ACGT A^CGT
MflI 285 AGATCT [GA]^GATC[CT]
MroI 455 TCCGGA T^CCGGA
MslI 453 CATCCGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 456 CCGG C^CGG
MvnI 375 CGCG CG^CG
NarI 235 GGCGCC GG^CGCC
NspI 66 GCATGT [GA]CATG^[CT]
PleI 538 GAGTC GAGTC(4/5)
PmaCI 574 CACGTG CAC^GTG
PmlI 574 CACGTG CAC^GTG
RcaI 320 TCATGA T^CATGA
SfoI 235 GGCGCC GGC^GCC
TaqI 314 TCGA T^CGA
TfiI 9 GAATC G^A[AT]TC
Tsp509I 429 AATT ^AATT
TthHB8I 314 TCGA T^CGA
XbaI 288 TCTAGA T^CTAGA
XhoII 285 AGATCT [GA]^GATC[CT]
XmnI 270 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 130 CCGC CCGC(-3/-1)
374 CCGC
AvaII 42 GGTCC G^G[AT]CC
445 GGACC
BanI 77 GGTACC G^G[CT][GA]CC
235 GGCGCC
BanII 331 GAGCCC G[GA]GC[CT]^C
610 GAGCCC
BfaI 289 CTAG C^TAG
403 CTAG
BpmI 139 CTGGAG CTGGAG(16/14)
559 CTGGAG
Cac8I 167 GCTGGC GC[ACGT]^[ACGT]GC
222 GCAGGC
DdeI 362 CTGAG C^T[ACGT]AG
520 CTCAG
DpnII 286 GATC ^GATC
312 GATC
EcoT14I 302 CCTTGG C^C[AT][AT]GG
354 CCTTGG
HinfI 9 GAATC G^A[ACGT]TC
538 GAGTC
HphI 513 GGTGA GGTGA(8/7)
299 TCACC
MaeI 289 CTAG C^TAG
403 CTAG
MaeIII 394 GTGAC ^GT[ACGT]AC
514 GTGAC
MboI 286 GATC ^GATC
312 GATC
NdeII 286 GATC ^GATC
312 GATC
PstI 106 CTGCAG CTGCA^G
339 CTGCAG
PvuII 165 CAGCTG CAG^CTG
336 CAGCTG
Sau3AI 286 GATC ^GATC
312 GATC
SfcI 106 CTGCAG C^T[GA][CT]AG
339 CTGCAG
StyI 302 CCTTGG C^C[AT][AT]GG
354 CCTTGG
Tsp45I 394 GTGAC ^GT[GC]AC
514 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AfaI AluI BbvI BmyI BsaJI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI Cfr13I EarI EcoRII Fnu4HI
FokI HaeIII ItaI Ksp632I MboII
MnlI MspA1I MvaI MwoI NlaIII
NlaIV RsaI Sau96I ScrFI SfaNI
TseI TspRI