################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI AlwI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp718I AspEI AspI AvrII BamHI BbvCI BcgI BciVI BfaI BfrI BglII BlnI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI HincII HindII HindIII HpaI KpnI MaeI MamI MfeI MflI MluI MroI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI StyI SwaI TfiI Tru9I Tth111I Van91I XbaI XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### AcyI 657 GGCGCC G[GA]^CG[CT]C AflIII 332 ACATGT A^C[GA][CT]GT Alw44I 595 GTGCAC G^TGCAC AlwNI 244 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 595 GTGCAC G^TGCAC Asp700I 141 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 595 GTGCAC G[AT]GC[AT]^C AvaI 653 CCCGGG C^[CT]CG[GA]G AviII 368 TGCGCA TGC^GCA BalI 530 TGGCCA TGG^CCA BbeI 657 GGCGCC GGCGC^C BbrPI 526 CACGTG CAC^GTG BclI 752 TGATCA T^GATCA BlpI 225 GCTGAGC GC^T[ACGT]AGC Bpu1102I 225 GCTGAGC GC^T[ACGT]AGC BsaHI 657 GGCGCC G[GA]^CG[CT]C BsaI 103 GAGACC GGTCTC(1/5) BsgI 40 CTGCAC GTGCAG(16/14) BsiHKAI 595 GTGCAC G[AT]GC[AT]^C BsmFI 188 GGGAC GGGAC(10/14) BsoBI 653 CCCGGG C^[CT]CG[GA]G BspLU11I 332 ACATGT A^CATGT BstAPI 216 GCACAATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 428 CCAGCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 225 GCTGAGC GC^T[ACGT]AGC DpnII 753 GATC ^GATC DraIII 217 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 325 CTCTTC CTCTTC(1/4) FbaI 752 TGATCA T^GATCA FspI 368 TGCGCA TGC^GCA HaeII 657 GGCGCC [GA]GCGC^[CT] HgaI 377 GCGTC GACGC(5/10) Hin1I 657 GGCGCC G[GA]^CG[CT]C HinfI 424 GAGTC G^A[ACGT]TC HphI 473 TCACC GGTGA(8/7) KasI 657 GGCGCC G^GCGCC Ksp632I 325 CTCTTC CTCTTC(1/4) KspI 558 CCGCGG CCGC^GG MaeIII 121 GTGAC ^GT[ACGT]AC MboI 753 GATC ^GATC MluNI 530 TGGCCA TGG^CCA MscI 530 TGGCCA TGG^CCA NaeI 174 GCCGGC GCC^GGC NarI 657 GGCGCC GG^CGCC NdeII 753 GATC ^GATC NgoMIV 174 GCCGGC G^CCGGC PleI 424 GAGTC GAGTC(4/5) PmaCI 526 CACGTG CAC^GTG PmlI 526 CACGTG CAC^GTG PpuMI 188 GGGACCT [GA]G^G[AT]CC[CT] PstI 763 CTGCAG CTGCA^G SacII 558 CCGCGG CCGC^GG Sau3AI 753 GATC ^GATC SfcI 763 CTGCAG C^T[GA][CT]AG SfoI 657 GGCGCC GGC^GCC SmaI 653 CCCGGG CCC^GGG SmlI 211 CTCAAG C^T[CT][GA]AG StuI 575 AGGCCT AGG^CCT TaqI 449 TCGA T^CGA Tsp45I 121 GTGAC ^GT[GC]AC TthHB8I 449 TCGA T^CGA XmaCI 653 CCCGGG C^CCGGG XmaI 653 CCCGGG C^CCGGG XmnI 141 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AfaI 246 GTAC GT^AC 516 GTAC BanI 554 GGCACC G^G[CT][GA]CC 657 GGCGCC BbsI 145 GTCTTC GAAGAC(2/6) 379 GTCTTC BcnI 653 CCCGG CC^[GC]GG 654 CCGGG BglI 349 GCCCAGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 581 GCCAGGAGGGC BpmI 436 CTGGAG CTGGAG(16/14) 622 CTGGAG BpuAI 145 GTCTTC GAAGAC(2/6) 379 GTCTTC BsaAI 313 TACGTG [CT]AC^GT[GA] 526 CACGTG BsmAI 21 GAGAC GTCTC(1/5) 103 GAGAC BspMI 761 ACCTGC ACCTGC(4/8) 269 GCAGGT BsrFI 174 GCCGGC [GA]^CCGG[CT] 548 ACCGGC BsrI 599 ACTGG ACTGG(1/-1) 513 CCAGT BstF5I 321 CATCC GGATG(2/0) 522 CATCC Cfr10I 174 GCCGGC [GA]^CCGG[CT] 548 ACCGGC DraII 188 GGGACCT [GA]G^G[ACGT]CC[CT] 452 AGGGCCT EaeI 510 CGGCCA [CT]^GGCC[GA] 530 TGGCCA EcoO109I 188 GGGACCT [GA]G^G[ACGT]CC[CT] 452 AGGGCCT FauI 354 GCGGG CCCGC(4/6) 358 GCGGG FokI 321 CATCC GGATG(9/13) 522 CATCC MaeII 314 ACGT A^CGT 527 ACGT MslI 68 CACTAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 522 CATCCACGTG NciI 653 CCCGG CC^[GC]GG 654 CCGGG PvuII 183 CAGCTG CAG^CTG 388 CAGCTG RsaI 246 GTAC GT^AC 516 GTAC SfaNI 521 GCATC GCATC(5/9) 590 GCATC Tsp509I 80 AATT ^AATT 254 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AccII AciI AluI AvaII BanII BbvI BmyI BsaJI BseRI BsiYI BslI Bsp1286I BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DsaI EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MboII MnlI MspA1I MspI MvaI MvnI MwoI NlaIII NlaIV NspI Sau96I ScrFI SphI TseI TspRI