(Circular) (Six-base) MAPSORT of: pFN21AE1697.seq Check: 1248 from: 1 to: 5494 pFN21AE1697 5494 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3304 278 Size: 53 83 186 936 1768 2468 Fragments arranged by size: 2468 1768 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2837 1937 Size: 900 4594 Acc65I G'GTAC_C Cuts at: 2815 2815 Size: 5494 AclI AA'CG_TT Cuts at: 1563 3621 3994 1563 Size: 2058 373 3063 Fragments arranged by size: 3063 2058 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2253 3557 4941 5209 1632 Size: 621 1304 1384 268 1917 Fragments arranged by size: 1917 1384 1304 621 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5297 AflIII A'CryG_T Cuts at: 1203 2337 4393 5370 1203 Size: 1134 2056 977 1327 Fragments arranged by size: 2056 1327 1134 977 AgeI A'CCGG_T Cuts at: 4325 4325 Size: 5494 AhdI GACnn_n'nnGTC Cuts at: 4223 4223 Size: 5494 AleI CACnn'nnGTG Cuts at: 1166 2077 1166 Size: 911 4583 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5494 AlwNI CAG_nnn'CTG Cuts at: 2254 3237 4809 2254 Size: 983 1572 2939 Fragments arranged by size: 2939 1572 983 ApaLI G'TGCA_C Cuts at: 2600 3551 4707 2600 Size: 951 1156 3387 Fragments arranged by size: 3387 1156 951 ApoI r'AATT_y Cuts at: 1493 2803 3029 3181 5290 1493 Size: 1310 226 152 2109 1697 Fragments arranged by size: 2109 1697 1310 226 152 AseI AT'TA_AT Cuts at: 160 1032 4048 160 Size: 872 3016 1606 Fragments arranged by size: 3016 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5494 AvaI C'yCGr_G Cuts at: 1870 1943 2658 2819 1870 Size: 73 715 161 4545 Fragments arranged by size: 4545 715 161 73 BamHI G'GATC_C Cuts at: 2824 3191 2824 Size: 367 5127 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2559 2662 2815 4270 618 Size: 325 217 627 772 103 153 1455 1842 Fragments arranged by size: 1842 1455 772 627 325 217 153 103 BanII G_rGCy'C Cuts at: 1391 2237 2369 2699 2813 1391 Size: 846 132 330 114 4072 Fragments arranged by size: 4072 846 330 132 114 BbeI G_GCGC'C Cuts at: 2666 2666 Size: 5494 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2143 2377 928 Size: 971 244 234 4045 Fragments arranged by size: 4045 971 244 234 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3685 3719 1903 Size: 34 1748 34 3678 Fragments arranged by size: 3678 1748 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3386 4596 3386 Size: 1210 4284 BclI T'GATC_A Cuts at: 1548 2757 1548 Size: 1209 4285 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2360 2592 4105 Size: 107 122 71 631 605 688 232 1513 Cuts at: 4105 136 Size: 1525 Fragments arranged by size: 1525 1513 688 631 605 232 122 107 71 BglII A'GATC_T Cuts at: 5489 5489 Size: 5494 BlpI GC'TnA_GC Cuts at: 2231 2911 2231 Size: 680 4814 Bme1580I G_kGCm'C Cuts at: 1790 2604 3555 4711 1790 Size: 814 951 1156 2573 Fragments arranged by size: 2573 1156 951 814 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5494 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3225 4183 442 Size: 416 53 83 399 24 1808 958 1753 Fragments arranged by size: 1808 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5494 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2462 2648 4154 1239 Size: 1223 186 1506 2579 Fragments arranged by size: 2579 1506 1223 186 Bpu10I CC'TnA_GC Cuts at: 2698 2698 Size: 5494 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2201 3619 4484 4782 2201 Size: 1418 865 298 2913 Fragments arranged by size: 2913 1418 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2102 4157 882 Size: 617 603 2055 2219 Fragments arranged by size: 2219 2055 617 603 BsaAI yAC'GTr Cuts at: 493 1204 2320 2533 3285 493 Size: 711 1116 213 752 2702 Fragments arranged by size: 2702 1116 752 711 213 BsaBI GATnn'nnATC Cuts at: 5488 5488 Size: 5494 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2663 3301 3683 4318 Size: 53 83 186 936 1130 638 382 635 Cuts at: 4318 275 Size: 1451 Fragments arranged by size: 1451 1130 936 638 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3927 4325 4599 4746 5157 3927 Size: 398 274 147 411 4264 Fragments arranged by size: 4264 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2639 2669 1178 Size: 30 165 30 1236 30 4003 Fragments arranged by size: 4003 1236 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2183 2459 2645 2648 1186 Size: 997 276 186 3 4032 Fragments arranged by size: 4032 997 276 186 3 BseYI C'CCAG_C Cuts at: 2190 2355 2612 2666 4697 5193 2190 Size: 165 257 54 2031 496 2491 Fragments arranged by size: 2491 2031 496 257 165 54 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2030 1163 Size: 573 294 4627 Fragments arranged by size: 4627 573 294 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2944 3705 4733 1540 Size: 275 186 943 761 1028 2301 Fragments arranged by size: 2301 1028 943 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2604 3555 3640 4711 2604 Size: 951 85 1071 3387 Fragments arranged by size: 3387 1071 951 85 BsmI GAATG_Cn' Cuts at: 3005 3098 3005 Size: 93 5401 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2237 2369 2604 2699 2813 3555 3640 Size: 399 447 132 235 95 114 742 85 Cuts at: 3640 4711 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 447 399 235 132 114 95 85 BspEI T'CCGG_A Cuts at: 5157 5157 Size: 5494 BspHI T'CATG_A Cuts at: 3383 5113 3383 Size: 1730 3764 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2265 2775 2849 844 Size: 1421 510 74 3489 Fragments arranged by size: 3489 1421 510 74 BsrBI CCG'CTC Cuts at: 1394 1823 3381 1394 Size: 429 1558 3507 Fragments arranged by size: 3507 1558 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3989 4163 65 Size: 1183 2741 174 1396 Fragments arranged by size: 2741 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2179 2553 4138 4325 1700 Size: 110 369 374 1585 187 2869 Fragments arranged by size: 2869 1585 374 369 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5494 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5494 BssSI C'ACGA_G Cuts at: 3554 4566 3554 Size: 1012 4482 BstAPI GCAn_nnn'nTGC Cuts at: 794 2227 794 Size: 1433 4061 BstBI TT'CG_AA Cuts at: 4298 4298 Size: 5494 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5494 BstXI CCAn_nnnn'nTGG Cuts at: 2440 2440 Size: 5494 BstYI r'GATC_y Cuts at: 1972 2824 3191 3576 3593 5045 5131 5143 5489 Size: 852 367 385 17 1452 86 12 346 Cuts at: 5489 1972 Size: 1977 Fragments arranged by size: 1977 1452 852 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2021 2563 2742 513 Size: 552 956 542 179 3265 Fragments arranged by size: 3265 956 552 542 179 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4755 Fragments arranged by size: 4755 683 56 BtsI GCAGTG_nn' Cuts at: 2281 2501 3010 3804 3824 2281 Size: 220 509 794 20 3951 Fragments arranged by size: 3951 794 509 220 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5494 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5459 DraI TTT'AAA Cuts at: 2798 2798 Size: 5494 DraIII CAC_nnn'GTG Cuts at: 2227 2227 Size: 5494 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4501 809 Size: 507 3185 1802 Fragments arranged by size: 3185 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2515 2535 2941 Size: 54 1425 50 275 18 685 20 406 Cuts at: 2941 3830 5459 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 685 406 275 54 50 43 20 18 EagI C'GGCC_G Cuts at: 1537 1812 2941 1537 Size: 275 1129 4090 Fragments arranged by size: 4090 1129 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2336 3424 1353 Size: 983 1088 3423 Fragments arranged by size: 3423 1088 983 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4081 4455 4601 387 Size: 1064 138 2492 374 146 1280 Fragments arranged by size: 2492 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2253 2462 2648 3557 4154 4941 5209 Size: 393 621 209 186 909 597 787 268 Cuts at: 5209 1239 Size: 1524 Fragments arranged by size: 1524 909 787 621 597 393 268 209 186 EcoO109I rG'GnC_Cy Cuts at: 2194 2458 2194 Size: 264 5230 EcoRI G'AATT_C Cuts at: 2803 3181 2803 Size: 378 5116 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2167 2167 Size: 5494 FspI TGC'GCA Cuts at: 2375 4000 2375 Size: 1625 3869 FspAI rTGC'GCAy Cuts at: 2375 2375 Size: 5494 HaeII r_GCGC'y Cuts at: 1678 2666 4641 1678 Size: 988 1975 2531 Fragments arranged by size: 2531 1975 988 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4135 4167 4209 4241 1373 Size: 32 135 32 2563 32 42 32 2626 Fragments arranged by size: 2626 2563 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2838 3084 669 Size: 988 281 900 246 3079 Fragments arranged by size: 3079 988 900 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5494 HpaI GTT'AAC Cuts at: 3084 3084 Size: 5494 KasI G'GCGC_C Cuts at: 2662 2662 Size: 5494 KpnI G_GTAC'C Cuts at: 2819 2819 Size: 5494 MfeI C'AATT_G Cuts at: 3093 3093 Size: 5494 MluI A'CGCG_T Cuts at: 5370 5370 Size: 5494 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2222 4608 4792 1305 Size: 917 2386 184 2007 Fragments arranged by size: 2386 2007 917 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2537 10 Size: 54 1425 343 705 2967 Fragments arranged by size: 2967 1425 705 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2077 2531 3452 3811 3970 518 Size: 648 911 454 921 359 159 2042 Fragments arranged by size: 2042 921 911 648 454 359 159 MspA1I CmG'CkG Cuts at: 1606 2190 2359 2395 2565 2910 3587 4735 4980 Size: 584 169 36 170 345 677 1148 245 Cuts at: 4980 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 584 345 245 170 169 36 NaeI GCC'GGC Cuts at: 2181 2181 Size: 5494 NarI GG'CG_CC Cuts at: 2663 2663 Size: 5494 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4942 NdeI CA'TA_TG Cuts at: 387 387 Size: 5494 NgoMIV G'CCGG_C Cuts at: 2179 2179 Size: 5494 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5494 NotI GC'GGCC_GC Cuts at: 2941 2941 Size: 5494 NspI r_CATG'y Cuts at: 1132 1229 2267 2341 2380 2440 2852 4397 1132 Size: 97 1038 74 39 60 412 1545 2229 Fragments arranged by size: 2229 1545 1038 412 97 74 60 39 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5494 PciI A'CATG_T Cuts at: 2337 4393 2337 Size: 2056 3438 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5494 PfoI T'CCnGG_A Cuts at: 2343 2343 Size: 5494 PmeI GTTT'AAAC Cuts at: 2798 2798 Size: 5494 PmlI CAC'GTG Cuts at: 2533 2533 Size: 5494 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2699 2731 3563 3595 5076 5108 2699 Size: 32 832 32 1481 32 3085 Fragments arranged by size: 3085 1481 832 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2194 2194 Size: 5494 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5494 PsiI TTA'TAA Cuts at: 3064 3064 Size: 5494 PstI C_TGCA'G Cuts at: 830 2772 2846 830 Size: 1942 74 3478 Fragments arranged by size: 3478 1942 74 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5494 PvuII CAG'CTG Cuts at: 2190 2395 2190 Size: 205 5289 SacII CC_GC'GG Cuts at: 2566 2566 Size: 5494 SalI G'TCGA_C Cuts at: 1936 2836 1936 Size: 900 4594 SbfI CC_TGCA'GG Cuts at: 2846 2846 Size: 5494 ScaI AGT'ACT Cuts at: 3742 5454 3742 Size: 1712 3782 SfcI C'TryA_G Cuts at: 826 1045 2768 2842 3977 4658 4849 826 Size: 219 1723 74 1135 681 191 1471 Fragments arranged by size: 1723 1471 1135 681 219 191 74 SfoI GGC'GCC Cuts at: 2664 2664 Size: 5494 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5494 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5494 SmaI CCC'GGG Cuts at: 1872 2660 2821 1872 Size: 788 161 4545 Fragments arranged by size: 4545 788 161 SmlI C'TyrA_G Cuts at: 820 1017 1943 2216 3598 4499 4761 820 Size: 197 926 273 1382 901 262 1553 Fragments arranged by size: 1553 1382 926 901 273 262 197 SnaBI TAC'GTA Cuts at: 493 3285 493 Size: 2792 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5494 SphI G_CATG'C Cuts at: 1132 2267 2380 2440 2852 1132 Size: 1135 113 60 412 3774 Fragments arranged by size: 3774 1135 412 113 60 SspI AAT'ATT Cuts at: 5 52 3418 5 Size: 47 3366 2081 Fragments arranged by size: 3366 2081 47 StuI AGG'CCT Cuts at: 1517 2582 1517 Size: 1065 4429 StyI C'CwwG_G Cuts at: 513 1065 2933 513 Size: 552 1868 3074 Fragments arranged by size: 3074 1868 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3521 3538 3691 3876 4321 325 Size: 1255 1941 17 153 185 445 1498 Fragments arranged by size: 1941 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3740 5452 96 Size: 274 80 33 51 1443 1763 1712 138 Fragments arranged by size: 1763 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5454 5486 5454 Size: 32 5462 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5494 XbaI T'CTAG_A Cuts at: 2830 2830 Size: 5494 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5494 XmaI C'CCGG_G Cuts at: 1870 2658 2819 1870 Size: 788 161 4545 Fragments arranged by size: 4545 788 161 XmnI GAAnn'nnTTC Cuts at: 2150 3623 2150 Size: 1473 4021 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3302 276 Size: 53 83 186 936 1768 2468 Fragments arranged by size: 2468 1768 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI