Restriction map for
pFN21AB0452
(Linear) (Six-base) MAPSORT of: KIBB0452.seq Check: 9693 from: 1 to: 2757
KIBB0452.orf
With 182 enzymes: SgfI *
November 15, 2011 13:19 ..
AccI GT'mk_AC
Cuts at: 0 29 1021 2757
Size: 29 992 1736
Fragments arranged by size:
1736 992 29
Acc65I G'GTAC_C
Cuts at: 0 1505 2757
Size: 1505 1252
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 923 1781 1912 2421 2757
Size: 559 364 858 131 509 336
Fragments arranged by size:
858 559 509 364 336 131
AfeI AGC'GCT
Cuts at: 0 984 2757
Size: 984 1773
AflII C'TTAA_G
Cuts at: 0 513 2757
Size: 513 2244
AflIII A'CryG_T
Cuts at: 0 1723 2139 2184 2757
Size: 1723 416 45 573
Fragments arranged by size:
1723 573 416 45
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 442 2621 2757
Size: 442 2179 136
Fragments arranged by size:
2179 442 136
AlwNI CAG_nnn'CTG
Cuts at: 0 75 397 438 1187 1464 1974 2757
Size: 75 322 41 749 277 510 783
Fragments arranged by size:
783 749 510 322 277 75 41
ApaI G_GGCC'C
Cuts at: 0 115 499 1573 2757
Size: 115 384 1074 1184
Fragments arranged by size:
1184 1074 384 115
ApoI r'AATT_y
Cuts at: 0 1813 2407 2473 2757
Size: 1813 594 66 284
Fragments arranged by size:
1813 594 284 66
AvaI C'yCGr_G
Cuts at: 0 108 373 636 2757
Size: 108 265 263 2121
Fragments arranged by size:
2121 265 263 108
AvrII C'CTAG_G
Cuts at: 0 966 2757
Size: 966 1791
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1759 1792 2757
Size: 1759 33 965
Fragments arranged by size:
1759 965 33
BanI G'GyrC_C
Cuts at: 0 119 151 1505 2363 2757
Size: 119 32 1354 858 394
Fragments arranged by size:
1354 858 394 119 32
BanII G_rGCy'C
Cuts at: 0 115 499 645 1000 1573 1719 1884 2231
Size: 115 384 146 355 573 146 165 347
Cuts at: 2231 2757
Size: 526
Fragments arranged by size:
573 526 384 355 347 165 146 146
115
BbeI G_GCGC'C
Cuts at: 0 155 2757
Size: 155 2602
BbsI GAAGACnn'nnnn_
Cuts at: 0 1675 1734 1837 2757
Size: 1675 59 103 920
Fragments arranged by size:
1675 920 103 59
BbvCI CC'TCA_GC
Cuts at: 0 1275 2757
Size: 1275 1482
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1045 1079 2071 2105 2598 2632 2757
Size: 1045 34 992 34 493 34 125
Fragments arranged by size:
1045 992 493 125 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 609 2757
Size: 609 2148
BglI GCCn_nnn'nGGC
Cuts at: 0 308 409 640 853 1192 1238 2757
Size: 308 101 231 213 339 46 1519
Fragments arranged by size:
1519 339 308 231 213 101 46
Bme1580I G_kGCm'C
Cuts at: 0 115 499 679 849 1535 1573 2711 2757
Size: 115 384 180 170 686 38 1138 46
Fragments arranged by size:
1138 686 384 180 170 115 46 38
BmrI ACTGGGnnnn_n'
Cuts at: 0 1535 2128 2757
Size: 1535 593 629
Fragments arranged by size:
1535 629 593
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 480 2712 2757
Size: 480 2232 45
Fragments arranged by size:
2232 480 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 419 1180 2259 2757
Size: 382 37 761 1079 498
Fragments arranged by size:
1079 761 498 382 37
Bpu10I CC'TnA_GC
Cuts at: 0 547 1275 2757
Size: 547 728 1482
Fragments arranged by size:
1482 728 547
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2357 2541 2757
Size: 2357 184 216
Fragments arranged by size:
2357 216 184
BsaAI yAC'GTr
Cuts at: 0 2142 2187 2757
Size: 2142 45 570
Fragments arranged by size:
2142 570 45
BsaHI Gr'CG_yC
Cuts at: 0 152 2757
Size: 152 2605
BsaWI w'CCGG_w
Cuts at: 0 450 1042 1591 2673 2757
Size: 450 592 549 1082 84
Fragments arranged by size:
1082 592 549 450 84
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 392 422 2757
Size: 392 30 2335
Fragments arranged by size:
2335 392 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 74 1972 2757
Size: 74 1898 785
Fragments arranged by size:
1898 785 74
BseYI C'CCAG_C
Cuts at: 0 18 492 2757
Size: 18 474 2265
Fragments arranged by size:
2265 474 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 1268 2617 2757
Size: 28 1240 1349 140
Fragments arranged by size:
1349 1240 140 28
BsiEI CG_ry'CG
Cuts at: 0 40 2517 2757
Size: 40 2477 240
Fragments arranged by size:
2477 240 40
BsiHKAI G_wGCw'C
Cuts at: 0 901 1000 1050 1719 2757
Size: 901 99 50 669 1038
Fragments arranged by size:
1038 901 669 99 50
BsmI GAATG_Cn'
Cuts at: 0 865 2757
Size: 865 1892
Bsp1286I G_dGCh'C
Cuts at: 0 115 499 645 679 849 901 1000 1050
Size: 115 384 146 34 170 52 99 50
Cuts at: 1050 1535 1573 1719 1884 2231 2711 2757
Size: 485 38 146 165 347 480 46
Fragments arranged by size:
485 480 384 347 170 165 146 146
115 99 52 50 46 38 34
BspEI T'CCGG_A
Cuts at: 0 450 1042 1591 2673 2757
Size: 450 592 549 1082 84
Fragments arranged by size:
1082 592 549 450 84
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1681 2645 2757
Size: 1681 964 112
Fragments arranged by size:
1681 964 112
BsrDI GCAATG_nn'
Cuts at: 0 2522 2757
Size: 2522 235
BsrGI T'GTAC_A
Cuts at: 0 2136 2339 2757
Size: 2136 203 418
Fragments arranged by size:
2136 418 203
BstAPI GCAn_nnn'nTGC
Cuts at: 0 438 2757
Size: 438 2319
BstBI TT'CG_AA
Cuts at: 0 2490 2757
Size: 2490 267
BstEII G'GTnAC_C
Cuts at: 0 1268 2757
Size: 1268 1489
BstXI CCAn_nnnn'nTGG
Cuts at: 0 738 1333 2586 2757
Size: 738 595 1253 171
Fragments arranged by size:
1253 738 595 171
BstYI r'GATC_y
Cuts at: 0 2714 2757
Size: 2714 43
Bsu36I CC'TnA_GG
Cuts at: 0 1457 2757
Size: 1457 1300
BtgI C'CryG_G
Cuts at: 0 407 731 2243 2757
Size: 407 324 1512 514
Fragments arranged by size:
1512 514 407 324
BtsI GCAGTG_nn'
Cuts at: 0 725 1213 2425 2757
Size: 725 488 1212 332
Fragments arranged by size:
1212 725 488 332
DraIII CAC_nnn'GTG
Cuts at: 0 2658 2757
Size: 2658 99
EaeI y'GGCC_r
Cuts at: 0 37 410 854 1106 2757
Size: 37 373 444 252 1651
Fragments arranged by size:
1651 444 373 252 37
EagI C'GGCC_G
Cuts at: 0 37 2757
Size: 37 2720
EarI CTCTTCn'nnn_
Cuts at: 0 1312 2440 2757
Size: 1312 1128 317
Fragments arranged by size:
1312 1128 317
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1115 2757
Size: 1115 1642
EcoICRI GAG'CTC
Cuts at: 0 998 1717 2757
Size: 998 719 1040
Fragments arranged by size:
1040 998 719
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 419 559 923 1180 1781 1912 2259
Size: 382 37 140 364 257 601 131 347
Cuts at: 2259 2421 2757
Size: 162 336
Fragments arranged by size:
601 382 364 347 336 257 162 140
131 37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 325 550 2757
Size: 325 225 2207
Fragments arranged by size:
2207 325 225
EcoO109I rG'GnC_Cy
Cuts at: 0 112 301 496 1221 1255 1570 2757
Size: 112 189 195 725 34 315 1187
Fragments arranged by size:
1187 725 315 195 189 112 34
EcoRI G'AATT_C
Cuts at: 0 2407 2757
Size: 2407 350
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1761 2757
Size: 1761 996
HaeII r_GCGC'y
Cuts at: 0 155 986 1187 2757
Size: 155 831 201 1570
Fragments arranged by size:
1570 831 201 155
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 371 403 1573 1605 2757
Size: 371 32 1170 32 1152
Fragments arranged by size:
1170 1152 371 32 32
HincII GTy'rAC
Cuts at: 0 2271 2757
Size: 2271 486
HindIII A'AGCT_T
Cuts at: 0 1694 2757
Size: 1694 1063
KasI G'GCGC_C
Cuts at: 0 151 2757
Size: 151 2606
KpnI G_GTAC'C
Cuts at: 0 1509 2757
Size: 1509 1248
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 734 2757
Size: 734 2023
MslI CAynn'nnrTG
Cuts at: 0 2698 2757
Size: 2698 59
MspA1I CmG'CkG
Cuts at: 0 438 469 521 529 617 826 1063 1170
Size: 438 31 52 8 88 209 237 107
Cuts at: 1170 1252 1759 2757
Size: 82 507 998
Fragments arranged by size:
998 507 438 237 209 107 88 82
52 31 8
NarI GG'CG_CC
Cuts at: 0 152 2757
Size: 152 2605
NcoI C'CATG_G
Cuts at: 0 731 2243 2757
Size: 731 1512 514
Fragments arranged by size:
1512 731 514
NruI TCG'CGA
Cuts at: 0 1079 2757
Size: 1079 1678
NspI r_CATG'y
Cuts at: 0 798 1611 1727 2325 2531 2757
Size: 798 813 116 598 206 226
Fragments arranged by size:
813 798 598 226 206 116
PciI A'CATG_T
Cuts at: 0 1723 2757
Size: 1723 1034
PflMI CCAn_nnn'nTGG
Cuts at: 0 397 2249 2455 2757
Size: 397 1852 206 302
Fragments arranged by size:
1852 397 302 206
PfoI T'CCnGG_A
Cuts at: 0 320 1000 2027 2218 2275 2332 2585 2757
Size: 320 680 1027 191 57 57 253 172
Fragments arranged by size:
1027 680 320 253 191 172 57 57
PmlI CAC'GTG
Cuts at: 0 2142 2187 2757
Size: 2142 45 570
Fragments arranged by size:
2142 570 45
PpuMI rG'GwC_Cy
Cuts at: 0 1221 1255 2757
Size: 1221 34 1502
Fragments arranged by size:
1502 1221 34
PspOMI G'GGCC_C
Cuts at: 0 111 495 1569 2757
Size: 111 384 1074 1188
Fragments arranged by size:
1188 1074 384 111
PstI C_TGCA'G
Cuts at: 0 173 990 2757
Size: 173 817 1767
Fragments arranged by size:
1767 817 173
PvuI CG_AT'CG
Cuts at: 0 2517 2757
Size: 2517 240
PvuII CAG'CTG
Cuts at: 0 438 469 521 1063 2757
Size: 438 31 52 542 1694
Fragments arranged by size:
1694 542 438 52 31
RsrII CG'GwC_CG
Cuts at: 0 1111 2757
Size: 1111 1646
SacI G_AGCT'C
Cuts at: 0 1000 1719 2757
Size: 1000 719 1038
Fragments arranged by size:
1038 1000 719
SanDI GG'GwC_CC
Cuts at: 0 1255 2757
Size: 1255 1502
ScaI AGT'ACT
Cuts at: 0 2215 2757
Size: 2215 542
SexAI A'CCwGG_T
Cuts at: 0 314 2757
Size: 314 2443
SfcI C'TryA_G
Cuts at: 0 169 986 1427 1915 2757
Size: 169 817 441 488 842
Fragments arranged by size:
842 817 488 441 169
SfoI GGC'GCC
Cuts at: 0 153 2757
Size: 153 2604
SmaI CCC'GGG
Cuts at: 0 110 2757
Size: 110 2647
SmlI C'TyrA_G
Cuts at: 0 513 2372 2556 2757
Size: 513 1859 184 201
Fragments arranged by size:
1859 513 201 184
SphI G_CATG'C
Cuts at: 0 2325 2531 2757
Size: 2325 206 226
Fragments arranged by size:
2325 226 206
StuI AGG'CCT
Cuts at: 0 2161 2757
Size: 2161 596
StyI C'CwwG_G
Cuts at: 0 656 701 731 966 2156 2243 2757
Size: 656 45 30 235 1190 87 514
Fragments arranged by size:
1190 656 514 235 87 45 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 137 730 1691 2757
Size: 137 593 961 1066
Fragments arranged by size:
1066 961 593 137
TatI w'GTAC_w
Cuts at: 0 2136 2213 2339 2757
Size: 2136 77 126 418
Fragments arranged by size:
2136 418 126 77
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 392 424 2398 2430 2757
Size: 392 32 1974 32 327
Fragments arranged by size:
1974 392 327 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1980 2757
Size: 1980 777
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 312 2757
Size: 312 2445
XmaI C'CCGG_G
Cuts at: 0 108 2757
Size: 108 2649
XmnI GAAnn'nnTTC
Cuts at: 0 76 779 2757
Size: 76 703 1978
Fragments arranged by size:
1978 703 76
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflII AflIII AlfI AlwNI
ApaI ApoI AvaI AvrII BaeI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BglI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI
BsrDI BsrGI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtsI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII
HindIII KasI KpnI MmeI MslI MspA1I NarI NcoI
NruI NspI PciI PflMI PfoI PmlI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SanDI ScaI SexAI
SfcI SfoI SmaI SmlI SphI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AgeI AhdI AleI AloI ApaLI
AscI AseI AsiSI BamHI BclI BfrBI BglII BlpI
BmgBI BmtI BsaI BsaBI BsiWI BsmBI BspHI BsrBI
BsrFI BssHII BssSI BstZ17I BtgZI ClaI CspCI DraI
DrdI EcoRV FseI FspI FspAI HpaI MfeI MluI
MscI NaeI NdeI NgoMIV NheI NotI NsiI PacI
PasI PmeI PpiI PshAI PsiI PspXI PsrI SacII
SalI SapI SbfI SfiI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI XbaI XhoI ZraI