################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## AatII AccII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AviII AvrII BalI BbeI BbrPI BcgI BciVI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsgI BsiWI BsmBI BsmFI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HgaI Hin1I HindIII KasI KspI MamI MfeI MluI MluNI MscI MspA1I MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Van91I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### Acc65I 29 GGTACC G^GTACC AccI 5 GTCGAC GT^[AC][GT]AC Asp718I 29 GGTACC G^GTACC AspI 1237 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 533 CTCGAG C^[CT]CG[GA]G BamHI 1828 GGATCC G^GATCC BanI 29 GGTACC G^G[CT][GA]CC BbsI 1366 GAAGAC GAAGAC(2/6) BclI 741 TGATCA T^GATCA BpuAI 1366 GAAGAC GAAGAC(2/6) BsmI 1302 GAATGC GAATGC(1/-1) BsoBI 533 CTCGAG C^[CT]CG[GA]G BspMI 648 GCAGGT ACCTGC(4/8) BsrDI 1050 CATTGC GCAATG(2/0) BsrI 1068 CCAGT ACTGG(1/-1) BstXI 230 CCATAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 84 GCGC GCG^C DraII 1447 AGGACCC [GA]G^G[ACGT]CC[CT] EcoO109I 1447 AGGACCC [GA]G^G[ACGT]CC[CT] FbaI 741 TGATCA T^GATCA HhaI 84 GCGC GCG^C HinP1I 84 GCGC G^CGC HpaI 202 GTTAAC GTT^AAC KpnI 29 GGTACC GGTAC^C MseI 203 TTAA T^TAA MslI 231 CATAAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 1701 CCATGG C^CATGG NspI 773 ACATGC [GA]CATG^[CT] PaeR7I 533 CTCGAG C^TCGAG PflFI 1237 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 1447 AGGACCC [GA]G^G[AT]CC[CT] SacI 1800 GAGCTC GAGCT^C SalI 5 GTCGAC G^TCGAC SexAI 614 ACCAGGT A^CC[AT]GGT Tru9I 203 TTAA T^TAA Tth111I 1237 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 533 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AlwNI 616 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG 1396 CAGGAGCTG AspHI 1515 GAGCAC G[AT]GC[AT]^C 1800 GAGCTC BbvCI 1054 GCTGAGG CCTCAGC(-5/-2) 1099 GCTGAGG BcnI 1782 CCGGG CC^[GC]GG 1824 CCGGG BfaI 242 CTAG C^TAG 665 CTAG BglII 1225 AGATCT A^GATCT 1253 AGATCT BsiEI 1641 CGGCCG CG[GA][CT]^CG 1779 CGGCCG BsiHKAI 1515 GAGCAC G[AT]GC[AT]^C 1800 GAGCTC DsaI 1644 CCGTGG C^C[GA][CT]GG 1701 CCATGG EagI 1641 CGGCCG C^GGCCG 1779 CGGCCG EclXI 1641 CGGCCG C^GGCCG 1779 CGGCCG Eco52I 1641 CGGCCG C^GGCCG 1779 CGGCCG EcoNI 1350 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1452 CCTCCTGGAGG FauI 1376 CCCGC CCCGC(4/6) 1796 GCGGG HincII 5 GTCGAC GT[CT]^[GA]AC 202 GTTAAC HindII 5 GTCGAC GT[CT]^[GA]AC 202 GTTAAC HphI 525 GGTGA GGTGA(8/7) 591 GGTGA MaeI 242 CTAG C^TAG 665 CTAG MaeII 255 ACGT A^CGT 1499 ACGT NciI 1782 CCGGG CC^[GC]GG 1824 CCGGG SapI 1089 GAAGAGC GCTCTTC(1/4) 1132 GAAGAGC SmlI 533 CTCGAG C^T[CT][GA]AG 1309 CTCAAG TfiI 757 GATTC G^A[AT]TC 1203 GAATC XcmI 1361 CCAAGGAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1412 CCAAGCTGGCCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AccIII AciI AcsI AfaI AluI AlwI ApoI AvaII BanII BbvI BmyI BpmI BsaJI BsaWI BseAI BseRI BsiYI BslI BsmAI Bsp1286I BspEI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI Ksp632I MaeIII MboI MboII MflI MnlI MroI MspI MvaI MwoI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII