(Circular) (Six-base) MAPSORT of: pFN21AB6156.seq Check: 2341 from: 1 to: 6616 pFN21AB6156 6616 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4426 278 Size: 53 83 186 936 2890 2468 Fragments arranged by size: 2890 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2011 3959 1937 Size: 74 1948 4594 Fragments arranged by size: 4594 1948 74 Acc65I G'GTAC_C Cuts at: 2034 3937 2034 Size: 1903 4713 AclI AA'CG_TT Cuts at: 1563 4743 5116 1563 Size: 3180 373 3063 Fragments arranged by size: 3180 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2038 2474 2759 2792 2891 4679 6063 6331 Size: 406 436 285 33 99 1788 1384 268 Cuts at: 6331 1632 Size: 1917 Fragments arranged by size: 1917 1788 1384 436 406 285 268 99 33 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6419 AflIII A'CryG_T Cuts at: 1203 5515 6492 1203 Size: 4312 977 1327 Fragments arranged by size: 4312 1327 977 AgeI A'CCGG_T Cuts at: 5447 5447 Size: 6616 AhdI GACnn_n'nnGTC Cuts at: 5345 5345 Size: 6616 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6616 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3168 1184 Size: 1984 4632 AlwNI CAG_nnn'CTG Cuts at: 2626 3406 4359 5931 2626 Size: 780 953 1572 3311 Fragments arranged by size: 3311 1572 953 780 ApaLI G'TGCA_C Cuts at: 4673 5829 4673 Size: 1156 5460 ApoI r'AATT_y Cuts at: 1493 2852 3181 3195 3925 4151 4303 6412 1493 Size: 1359 329 14 730 226 152 2109 1697 Fragments arranged by size: 2109 1697 1359 730 329 226 152 14 AseI AT'TA_AT Cuts at: 160 1032 5170 160 Size: 872 4138 1606 Fragments arranged by size: 4138 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6616 AvaI C'yCGr_G Cuts at: 1870 1943 2538 3941 1870 Size: 73 595 1403 4545 Fragments arranged by size: 4545 1403 595 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2026 2059 2026 Size: 33 6583 BamHI G'GATC_C Cuts at: 3833 3946 4313 3833 Size: 113 367 6136 Fragments arranged by size: 6136 367 113 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2034 3937 5392 618 Size: 325 217 627 247 1903 1455 1842 Fragments arranged by size: 1903 1842 1455 627 325 247 217 BanII G_rGCy'C Cuts at: 1391 2691 3794 3809 3935 1391 Size: 1300 1103 15 126 4072 Fragments arranged by size: 4072 1300 1103 126 15 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3378 928 Size: 971 1479 4166 Fragments arranged by size: 4166 1479 971 BbvCI CC'TCA_GC Cuts at: 3060 3105 3060 Size: 45 6571 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4807 4841 1903 Size: 34 2870 34 3678 Fragments arranged by size: 3678 2870 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4508 5718 4508 Size: 1210 5406 BclI T'GATC_A Cuts at: 1548 2746 1548 Size: 1198 5418 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5227 136 Size: 107 122 71 631 605 3555 1525 Fragments arranged by size: 3555 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3230 3258 6611 3230 Size: 28 3353 3235 Fragments arranged by size: 3353 3235 28 BlpI GC'TnA_GC Cuts at: 4033 4033 Size: 6616 Bme1580I G_kGCm'C Cuts at: 1790 3499 4677 5833 1790 Size: 1709 1178 1156 2573 Fragments arranged by size: 2573 1709 1178 1156 BmgBI CAC'GTC Cuts at: 1318 2261 1318 Size: 943 5673 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3067 4347 5305 Size: 416 53 83 399 24 1650 1280 958 Cuts at: 5305 442 Size: 1753 Fragments arranged by size: 1753 1650 1280 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6616 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2627 3365 3455 3482 3507 3606 3648 3708 Size: 1388 738 90 27 25 99 42 60 Cuts at: 3708 3777 3831 5276 1239 Size: 69 54 1445 2579 Fragments arranged by size: 2579 1445 1388 738 99 90 69 60 54 42 27 25 Bpu10I CC'TnA_GC Cuts at: 3060 3105 3060 Size: 45 6571 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3299 4741 5606 5904 3299 Size: 1442 865 298 4011 Fragments arranged by size: 4011 1442 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5279 882 Size: 617 3780 2219 Fragments arranged by size: 3780 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4407 493 Size: 711 3203 2702 Fragments arranged by size: 3203 2702 711 BsaBI GATnn'nnATC Cuts at: 6610 6610 Size: 6616 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4423 4805 5440 275 Size: 53 83 186 936 2890 382 635 1451 Fragments arranged by size: 2890 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2802 3605 3665 3722 3902 5049 5447 5721 5868 Size: 803 60 57 180 1147 398 274 147 Cuts at: 5868 6279 2802 Size: 411 3139 Fragments arranged by size: 3139 1147 803 411 398 274 180 147 60 57 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6391 Fragments arranged by size: 6391 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2139 2265 3573 1186 Size: 953 126 1308 4229 Fragments arranged by size: 4229 1308 953 126 BseYI C'CCAG_C Cuts at: 2069 5819 6315 2069 Size: 3750 496 2370 Fragments arranged by size: 3750 2370 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6043 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3649 3787 4066 4827 5855 1540 Size: 275 186 1648 138 279 761 1028 2301 Fragments arranged by size: 2301 1648 1028 761 279 275 186 138 BsiHKAI G_wGCw'C Cuts at: 3524 3809 4677 4762 5833 3524 Size: 285 868 85 1071 4307 Fragments arranged by size: 4307 1071 868 285 85 BsmI GAATG_Cn' Cuts at: 3313 4127 4220 3313 Size: 814 93 5709 Fragments arranged by size: 5709 814 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2691 3499 3524 3794 3809 3935 4677 Size: 399 901 808 25 270 15 126 742 Cuts at: 4677 4762 5833 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 901 808 742 399 270 126 85 25 15 BspEI T'CCGG_A Cuts at: 3605 3665 3722 3902 6279 3605 Size: 60 57 180 2377 3942 Fragments arranged by size: 3942 2377 180 60 57 BspHI T'CATG_A Cuts at: 4505 6235 4505 Size: 1730 4886 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2644 3971 844 Size: 1800 1327 3489 Fragments arranged by size: 3489 1800 1327 BsrBI CCG'CTC Cuts at: 1394 1823 4503 1394 Size: 429 2680 3507 Fragments arranged by size: 3507 2680 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3054 5111 5285 65 Size: 1183 1806 2057 174 1396 Fragments arranged by size: 2057 1806 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5260 5447 1700 Size: 110 3450 187 2869 Fragments arranged by size: 3450 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6616 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6616 BssSI C'ACGA_G Cuts at: 4676 5688 4676 Size: 1012 5604 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6616 BstBI TT'CG_AA Cuts at: 5420 5420 Size: 6616 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6616 BstXI CCAn_nnnn'nTGG Cuts at: 2242 2242 Size: 6616 BstYI r'GATC_y Cuts at: 1972 2520 3230 3258 3430 3833 3946 4313 4698 Size: 548 710 28 172 403 113 367 385 Cuts at: 4698 4715 6167 6253 6265 6611 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 710 548 403 385 367 346 172 113 86 28 17 12 BtgI C'CryG_G Cuts at: 513 1065 3649 3706 513 Size: 552 2584 57 3423 Fragments arranged by size: 3423 2584 552 57 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5877 Fragments arranged by size: 5877 683 56 BtsI GCAGTG_nn' Cuts at: 2125 2380 3150 3739 4132 4926 4946 2125 Size: 255 770 589 393 794 20 3795 Fragments arranged by size: 3795 794 770 589 393 255 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6616 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6581 DraI TTT'AAA Cuts at: 3920 3920 Size: 6616 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5623 809 Size: 507 4307 1802 Fragments arranged by size: 4307 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3646 3703 3784 Size: 54 1425 50 275 18 1816 57 81 Cuts at: 3784 4063 4952 6581 8 Size: 279 889 1629 43 Fragments arranged by size: 1816 1629 1425 889 279 275 81 57 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3646 3784 4063 1537 Size: 275 1834 138 279 4090 Fragments arranged by size: 4090 1834 279 275 138 EarI CTCTTCn'nnn_ Cuts at: 1353 2735 3089 3132 4546 1353 Size: 1382 354 43 1414 3423 Fragments arranged by size: 3423 1414 1382 354 43 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5203 5577 5723 387 Size: 1064 138 3614 374 146 1280 Fragments arranged by size: 3614 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3807 3807 Size: 6616 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2038 2474 2627 2759 2792 2891 3365 Size: 393 406 436 153 132 33 99 474 Cuts at: 3365 3455 3482 3507 3606 3648 3708 3777 3831 Size: 90 27 25 99 42 60 69 54 Cuts at: 3831 4679 5276 6063 6331 1239 Size: 848 597 787 268 1524 Fragments arranged by size: 1524 848 787 597 474 436 406 393 268 153 132 99 99 90 69 60 54 42 33 27 25 EcoNI CCTnn'n_nnAGG Cuts at: 3359 3461 3359 Size: 102 6514 EcoO109I rG'GnC_Cy Cuts at: 3453 3453 Size: 6616 EcoRI G'AATT_C Cuts at: 3925 4303 3925 Size: 378 6238 FspI TGC'GCA Cuts at: 5122 5122 Size: 6616 HaeII r_GCGC'y Cuts at: 1678 5763 1678 Size: 4085 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2971 3003 3098 3130 3222 Size: 32 135 32 1399 32 95 32 92 Cuts at: 3222 3254 5257 5289 5331 5363 1373 Size: 32 2003 32 42 32 2626 Fragments arranged by size: 2626 2003 1399 135 95 92 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2012 2209 3960 4206 669 Size: 988 281 74 197 1751 246 3079 Fragments arranged by size: 3079 1751 988 281 246 197 74 HindIII A'AGCT_T Cuts at: 748 748 Size: 6616 HpaI GTT'AAC Cuts at: 2209 4206 2209 Size: 1997 4619 KpnI G_GTAC'C Cuts at: 2038 3941 2038 Size: 1903 4713 MfeI C'AATT_G Cuts at: 4215 4215 Size: 6616 MluI A'CGCG_T Cuts at: 6492 6492 Size: 6616 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2177 2739 3288 5730 5914 1305 Size: 872 562 549 2442 184 2007 Fragments arranged by size: 2442 2007 872 562 549 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4794 Fragments arranged by size: 4794 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2240 4574 4933 5092 518 Size: 648 1074 2334 359 159 2042 Fragments arranged by size: 2334 2042 1074 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4032 4709 5857 6102 1606 Size: 2426 677 1148 245 2120 Fragments arranged by size: 2426 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 3706 513 Size: 552 2641 3423 Fragments arranged by size: 3423 2641 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6616 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6616 NotI GC'GGCC_GC Cuts at: 4063 4063 Size: 6616 NspI r_CATG'y Cuts at: 1132 1229 2782 3974 5519 1132 Size: 97 1553 1192 1545 2229 Fragments arranged by size: 2229 1553 1545 1192 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6616 PciI A'CATG_T Cuts at: 5515 5515 Size: 6616 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6616 PfoI T'CCnGG_A Cuts at: 2604 3459 2604 Size: 855 5761 PmeI GTTT'AAAC Cuts at: 3920 3920 Size: 6616 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4685 4717 6198 6230 4685 Size: 32 1481 32 5071 Fragments arranged by size: 5071 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3453 3453 Size: 6616 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6616 PsiI TTA'TAA Cuts at: 4186 4186 Size: 6616 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3607 3639 3607 Size: 32 6584 PstI C_TGCA'G Cuts at: 830 2652 3147 3360 3968 830 Size: 1822 495 213 608 3478 Fragments arranged by size: 3478 1822 608 495 213 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6616 SacI G_AGCT'C Cuts at: 3809 3809 Size: 6616 SalI G'TCGA_C Cuts at: 1936 2010 3958 1936 Size: 74 1948 4594 Fragments arranged by size: 4594 1948 74 SapI GCTCTTCn'nnn_ Cuts at: 3089 3132 3089 Size: 43 6573 SbfI CC_TGCA'GG Cuts at: 3968 3968 Size: 6616 ScaI AGT'ACT Cuts at: 4864 6576 4864 Size: 1712 4904 SexAI A'CCwGG_T Cuts at: 2619 2619 Size: 6616 SfcI C'TryA_G Cuts at: 826 1045 2648 3143 3356 3448 3742 3964 5099 Size: 219 1603 495 213 92 294 222 1135 Cuts at: 5099 5780 5971 826 Size: 681 191 1471 Fragments arranged by size: 1603 1471 1135 681 495 294 222 219 213 191 92 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6616 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6616 SmaI CCC'GGG Cuts at: 1872 3943 1872 Size: 2071 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2538 3314 4720 5621 5883 820 Size: 197 926 595 776 1406 901 262 1553 Fragments arranged by size: 1553 1406 926 901 776 595 262 197 SnaBI TAC'GTA Cuts at: 493 4407 493 Size: 3914 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6616 SphI G_CATG'C Cuts at: 1132 3974 1132 Size: 2842 3774 SspI AAT'ATT Cuts at: 5 52 4540 5 Size: 47 4488 2081 Fragments arranged by size: 4488 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6616 StyI C'CwwG_G Cuts at: 513 1065 2212 2895 3366 3706 4055 513 Size: 552 1147 683 471 340 349 3074 Fragments arranged by size: 3074 1147 683 552 471 349 340 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4643 4660 4813 4998 5443 325 Size: 1255 3063 17 153 185 445 1498 Fragments arranged by size: 3063 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4862 6574 96 Size: 274 80 33 51 1443 2885 1712 138 Fragments arranged by size: 2885 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6576 6608 6576 Size: 32 6584 Tth111I GACn'n_nGTC Cuts at: 1316 3245 1316 Size: 1929 4687 XbaI T'CTAG_A Cuts at: 3952 3952 Size: 6616 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3373 3424 3373 Size: 51 6565 XhoI C'TCGA_G Cuts at: 1943 2538 1943 Size: 595 6021 XmaI C'CCGG_G Cuts at: 1870 3941 1870 Size: 2071 4545 XmnI GAAnn'nnTTC Cuts at: 4745 4745 Size: 6616 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4424 276 Size: 53 83 186 936 2890 2468 Fragments arranged by size: 2890 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PvuII RsrII SacII SanDI SfiI SfoI SrfI SwaI