(Circular) (Six-base) MAPSORT of: pFN21AE1398.seq Check: 4694 from: 1 to: 8011 pFN21AE1398 8011 bp With 182 enzymes: SgfI * June 19, 2019 18:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5821 278 Size: 53 83 186 936 4285 2468 Fragments arranged by size: 4285 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3015 3555 5354 1937 Size: 1078 540 1799 4594 Fragments arranged by size: 4594 1799 1078 540 Acc65I G'GTAC_C Cuts at: 5332 5332 Size: 8011 AclI AA'CG_TT Cuts at: 1563 5009 5310 6138 6511 1563 Size: 3446 301 828 373 3063 Fragments arranged by size: 3446 3063 828 373 301 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2221 2438 6074 7458 7726 1632 Size: 589 217 3636 1384 268 1917 Fragments arranged by size: 3636 1917 1384 589 268 217 AfeI AGC'GCT Cuts at: 2234 2234 Size: 8011 AflII C'TTAA_G Cuts at: 820 1017 4646 820 Size: 197 3629 4185 Fragments arranged by size: 4185 3629 197 AflIII A'CryG_T Cuts at: 1203 3546 3750 4254 6910 7887 1203 Size: 2343 204 504 2656 977 1327 Fragments arranged by size: 2656 2343 1327 977 504 204 AgeI A'CCGG_T Cuts at: 6842 6842 Size: 8011 AhdI GACnn_n'nnGTC Cuts at: 3022 3457 3517 6740 3022 Size: 435 60 3223 4293 Fragments arranged by size: 4293 3223 435 60 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8011 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8011 AlwNI CAG_nnn'CTG Cuts at: 5083 5754 7326 5083 Size: 671 1572 5768 Fragments arranged by size: 5768 1572 671 ApaLI G'TGCA_C Cuts at: 6068 7224 6068 Size: 1156 6855 ApoI r'AATT_y Cuts at: 1493 3185 3281 3308 3440 3861 4225 4239 5120 Size: 1692 96 27 132 421 364 14 881 Cuts at: 5120 5179 5320 5546 5698 7807 1493 Size: 59 141 226 152 2109 1697 Fragments arranged by size: 2109 1697 1692 881 421 364 226 152 141 132 96 59 27 14 AseI AT'TA_AT Cuts at: 160 1032 6565 160 Size: 872 5533 1606 Fragments arranged by size: 5533 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8011 AvaI C'yCGr_G Cuts at: 1870 1943 2063 4004 5336 1870 Size: 73 120 1941 1332 4545 Fragments arranged by size: 4545 1941 1332 120 73 BamHI G'GATC_C Cuts at: 5341 5708 5341 Size: 367 7644 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2942 2957 5332 6787 618 Size: 325 217 627 1155 15 2375 1455 1842 Fragments arranged by size: 2375 1842 1455 1155 627 325 217 15 BanII G_rGCy'C Cuts at: 1391 2064 3957 5241 5330 1391 Size: 673 1893 1284 89 4072 Fragments arranged by size: 4072 1893 1284 673 89 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4993 5169 928 Size: 971 3094 176 3770 Fragments arranged by size: 3770 3094 971 176 BbvCI CC'TCA_GC Cuts at: 3615 5030 3615 Size: 1415 6596 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3995 4029 6202 6236 1903 Size: 34 2058 34 2173 34 3678 Fragments arranged by size: 3678 2173 2058 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5028 5903 7113 5028 Size: 875 1210 5926 Fragments arranged by size: 5926 1210 875 BclI T'GATC_A Cuts at: 1548 2127 1548 Size: 579 7432 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6622 136 Size: 107 122 71 631 605 4950 1525 Fragments arranged by size: 4950 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4369 4591 8006 4369 Size: 222 3415 4374 Fragments arranged by size: 4374 3415 222 BlpI GC'TnA_GC Cuts at: 5428 5428 Size: 8011 Bme1580I G_kGCm'C Cuts at: 1790 2960 6072 7228 1790 Size: 1170 3112 1156 2573 Fragments arranged by size: 3112 2573 1170 1156 BmgBI CAC'GTC Cuts at: 1318 2388 3454 4255 1318 Size: 1070 1066 801 5074 Fragments arranged by size: 5074 1070 1066 801 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4151 5742 6700 Size: 416 53 83 399 24 2734 1591 958 Cuts at: 6700 442 Size: 1753 Fragments arranged by size: 2734 1753 1591 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8011 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2930 3849 2930 Size: 919 7092 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2345 2542 6671 1239 Size: 1106 197 4129 2579 Fragments arranged by size: 4129 2579 1106 197 Bpu10I CC'TnA_GC Cuts at: 2261 3615 5030 2261 Size: 1354 1415 5242 Fragments arranged by size: 5242 1415 1354 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4515 6136 7001 7299 4515 Size: 1621 865 298 5227 Fragments arranged by size: 5227 1621 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6674 882 Size: 617 5175 2219 Fragments arranged by size: 5175 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4145 5802 493 Size: 711 2941 1657 2702 Fragments arranged by size: 2941 2702 1657 711 BsaBI GATnn'nnATC Cuts at: 4047 4655 8005 4047 Size: 608 3350 4053 Fragments arranged by size: 4053 3350 608 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5818 6200 6835 275 Size: 53 83 186 936 4285 382 635 1451 Fragments arranged by size: 4285 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6444 6842 7116 7263 7674 6444 Size: 398 274 147 411 6781 Fragments arranged by size: 6781 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 5090 5120 1178 Size: 30 165 30 3687 30 4069 Fragments arranged by size: 4069 3687 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2186 3793 5281 1186 Size: 1000 1607 1488 3916 Fragments arranged by size: 3916 1607 1488 1000 BseYI C'CCAG_C Cuts at: 3146 7214 7710 3146 Size: 4068 496 3447 Fragments arranged by size: 4068 3447 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3517 3725 1163 Size: 573 1781 208 5449 Fragments arranged by size: 5449 1781 573 208 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5461 6222 7250 1540 Size: 275 186 3460 761 1028 2301 Fragments arranged by size: 3460 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3492 3957 6072 6157 7228 3492 Size: 465 2115 85 1071 4275 Fragments arranged by size: 4275 2115 1071 465 85 BsmI GAATG_Cn' Cuts at: 5522 5615 5522 Size: 93 7918 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 2960 3492 3957 5241 5330 6072 Size: 399 274 896 532 465 1284 89 742 Cuts at: 6072 6157 7228 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1284 1071 896 742 532 465 399 274 89 85 BspEI T'CCGG_A Cuts at: 7674 7674 Size: 8011 BspHI T'CATG_A Cuts at: 4051 5900 7630 4051 Size: 1849 1730 4432 Fragments arranged by size: 4432 1849 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5366 844 Size: 4522 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3676 5898 1394 Size: 429 1853 2222 3507 Fragments arranged by size: 3507 2222 1853 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2374 3194 3918 6506 6680 65 Size: 1183 1126 820 724 2588 174 1396 Fragments arranged by size: 2588 1396 1183 1126 820 724 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6655 6842 1700 Size: 110 4845 187 2869 Fragments arranged by size: 4845 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2587 2730 96 Size: 2491 143 5377 Fragments arranged by size: 5377 2491 143 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8011 BssSI C'ACGA_G Cuts at: 6071 7083 6071 Size: 1012 6999 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8011 BstBI TT'CG_AA Cuts at: 4915 6815 4915 Size: 1900 6111 BstEII G'GTnAC_C Cuts at: 1184 3595 1184 Size: 2411 5600 BstXI CCAn_nnnn'nTGG Cuts at: 2515 3731 2515 Size: 1216 6795 BstYI r'GATC_y Cuts at: 1972 3325 4369 4501 4591 5341 5708 6093 6110 Size: 1353 1044 132 90 750 367 385 17 Cuts at: 6110 7562 7648 7660 8006 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1353 1044 750 385 367 346 132 90 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3330 3330 Size: 8011 BtgI C'CryG_G Cuts at: 513 1065 2166 2291 2605 2977 3840 4788 513 Size: 552 1101 125 314 372 863 948 3736 Fragments arranged by size: 3736 1101 948 863 552 372 314 125 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7272 Fragments arranged by size: 7272 683 56 BtsI GCAGTG_nn' Cuts at: 2418 3529 5527 6321 6341 2418 Size: 1111 1998 794 20 4088 Fragments arranged by size: 4088 1998 1111 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8011 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7976 DraI TTT'AAA Cuts at: 4687 5119 5315 4687 Size: 432 196 7383 Fragments arranged by size: 7383 432 196 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7018 809 Size: 507 5702 1802 Fragments arranged by size: 5702 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3733 5458 6347 Size: 54 1425 50 275 18 1903 1725 889 Cuts at: 6347 7976 8 Size: 1629 43 Fragments arranged by size: 1903 1725 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5458 1537 Size: 275 3646 4090 Fragments arranged by size: 4090 3646 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3028 3636 4713 5941 1353 Size: 1675 608 1077 1228 3423 Fragments arranged by size: 3423 1675 1228 1077 608 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2609 6598 6972 7118 387 Size: 1064 138 1020 3989 374 146 1280 Fragments arranged by size: 3989 1280 1064 1020 374 146 138 EcoICRI GAG'CTC Cuts at: 3955 3955 Size: 8011 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2221 2345 2438 2542 6074 6671 7458 Size: 393 589 124 93 104 3532 597 787 Cuts at: 7458 7726 1239 Size: 268 1524 Fragments arranged by size: 3532 1524 787 597 589 393 268 124 104 93 EcoNI CCTnn'n_nnAGG Cuts at: 2544 4208 5232 5288 2544 Size: 1664 1024 56 5267 Fragments arranged by size: 5267 1664 1024 56 EcoO109I rG'GnC_Cy Cuts at: 3334 4762 3334 Size: 1428 6583 EcoRI G'AATT_C Cuts at: 3281 4239 5320 5698 3281 Size: 958 1081 378 5594 Fragments arranged by size: 5594 1081 958 378 EcoRV GAT'ATC Cuts at: 3052 3052 Size: 8011 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2790 2790 Size: 8011 FspI TGC'GCA Cuts at: 6517 6517 Size: 8011 HaeII r_GCGC'y Cuts at: 1678 2203 2236 7158 1678 Size: 525 33 4922 2531 Fragments arranged by size: 4922 2531 525 33 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2343 2372 2375 2404 2432 Size: 32 135 32 771 29 3 29 28 Cuts at: 2432 2464 2744 2776 3008 3040 3443 3475 3503 Size: 32 280 32 232 32 403 32 28 Cuts at: 3503 3535 3643 3675 4066 4098 4793 4825 6652 Size: 32 108 32 391 32 695 32 1827 Cuts at: 6652 6684 6726 6758 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1827 771 695 403 391 280 232 135 108 42 32 32 32 32 32 32 32 32 32 32 32 32 29 29 28 28 3 HincII GTy'rAC Cuts at: 669 1657 1938 2841 5355 5601 669 Size: 988 281 903 2514 246 3079 Fragments arranged by size: 3079 2514 988 903 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8011 HpaI GTT'AAC Cuts at: 2841 5601 2841 Size: 2760 5251 KpnI G_GTAC'C Cuts at: 5336 5336 Size: 8011 MfeI C'AATT_G Cuts at: 3483 5127 5610 3483 Size: 1644 483 5884 Fragments arranged by size: 5884 1644 483 MluI A'CGCG_T Cuts at: 7887 7887 Size: 8011 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 7125 7309 1305 Size: 5820 184 2007 Fragments arranged by size: 5820 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3735 10 Size: 54 1425 343 1903 4286 Fragments arranged by size: 4286 1903 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2075 2854 3495 4572 4752 5969 6328 Size: 648 909 779 641 1077 180 1217 359 Cuts at: 6328 6487 518 Size: 159 2042 Fragments arranged by size: 2042 1217 1077 909 779 648 641 359 180 159 MspA1I CmG'CkG Cuts at: 1606 2168 2979 5427 6104 7252 7497 1606 Size: 562 811 2448 677 1148 245 2120 Fragments arranged by size: 2448 2120 1148 811 677 562 245 NcoI C'CATG_G Cuts at: 513 1065 2291 2605 3840 513 Size: 552 1226 314 1235 4684 Fragments arranged by size: 4684 1235 1226 552 314 NdeI CA'TA_TG Cuts at: 387 387 Size: 8011 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8011 NotI GC'GGCC_GC Cuts at: 5458 5458 Size: 8011 NspI r_CATG'y Cuts at: 1132 1229 3550 3754 5369 6914 1132 Size: 97 2321 204 1615 1545 2229 Fragments arranged by size: 2321 2229 1615 1545 204 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8011 PciI A'CATG_T Cuts at: 3546 3750 6910 3546 Size: 204 3160 4647 Fragments arranged by size: 4647 3160 204 PflMI CCAn_nnn'nTGG Cuts at: 1108 2391 1108 Size: 1283 6728 PfoI T'CCnGG_A Cuts at: 3987 4423 4758 5094 3987 Size: 436 335 336 6904 Fragments arranged by size: 6904 436 336 335 PmeI GTTT'AAAC Cuts at: 5315 5315 Size: 8011 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6080 6112 7593 7625 6080 Size: 32 1481 32 6466 Fragments arranged by size: 6466 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3334 4762 3334 Size: 1428 6583 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8011 PsiI TTA'TAA Cuts at: 5581 5581 Size: 8011 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3144 3176 3144 Size: 32 7979 PstI C_TGCA'G Cuts at: 830 4518 5363 830 Size: 3688 845 3478 Fragments arranged by size: 3688 3478 845 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8011 SacI G_AGCT'C Cuts at: 3957 3957 Size: 8011 SacII CC_GC'GG Cuts at: 2169 2980 2169 Size: 811 7200 SalI G'TCGA_C Cuts at: 1936 5353 1936 Size: 3417 4594 SbfI CC_TGCA'GG Cuts at: 5363 5363 Size: 8011 ScaI AGT'ACT Cuts at: 6259 7971 6259 Size: 1712 6299 SexAI A'CCwGG_T Cuts at: 4938 4938 Size: 8011 SfcI C'TryA_G Cuts at: 826 1045 2690 2823 4514 4598 5359 6494 7175 Size: 219 1645 133 1691 84 761 1135 681 Cuts at: 7175 7366 826 Size: 191 1471 Fragments arranged by size: 1691 1645 1471 1135 761 681 219 191 133 84 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8011 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8011 SmaI CCC'GGG Cuts at: 1872 5338 1872 Size: 3466 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4530 4646 6115 7016 7278 820 Size: 197 926 2587 116 1469 901 262 1553 Fragments arranged by size: 2587 1553 1469 926 901 262 197 116 SnaBI TAC'GTA Cuts at: 493 5802 493 Size: 5309 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8011 SphI G_CATG'C Cuts at: 1132 5369 1132 Size: 4237 3774 SspI AAT'ATT Cuts at: 5 52 3984 5935 5 Size: 47 3932 1951 2081 Fragments arranged by size: 3932 2081 1951 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8011 StyI C'CwwG_G Cuts at: 513 1065 2291 2605 3739 3840 3974 5228 5450 Size: 552 1226 314 1134 101 134 1254 222 Cuts at: 5450 513 Size: 3074 Fragments arranged by size: 3074 1254 1226 1134 552 314 222 134 101 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2245 2549 2844 3012 4740 6038 6055 Size: 1255 665 304 295 168 1728 1298 17 Cuts at: 6055 6208 6393 6838 325 Size: 153 185 445 1498 Fragments arranged by size: 1728 1498 1298 1255 665 445 304 295 185 168 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2587 2730 3467 Size: 274 80 33 51 1443 610 143 737 Cuts at: 3467 5151 6257 7969 96 Size: 1684 1106 1712 138 Fragments arranged by size: 1712 1684 1443 1106 737 610 274 143 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3384 3416 7971 8003 3384 Size: 32 4555 32 3392 Fragments arranged by size: 4555 3392 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8011 XbaI T'CTAG_A Cuts at: 5347 5347 Size: 8011 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8011 XmaI C'CCGG_G Cuts at: 1870 5336 1870 Size: 3466 4545 XmnI GAAnn'nnTTC Cuts at: 6140 6140 Size: 8011 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5819 276 Size: 53 83 186 936 4285 2468 Fragments arranged by size: 4285 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SacI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PvuII RsrII SanDI SapI SfiI SfoI SrfI SwaI XcmI