################################## # NO CUT ENZYMES ( 160 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI Aor51HI AscI AseI AsnI Asp700I Asp718I AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BcnI BfaI BfrI BglII BlnI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI KspI MaeI MamI MfeI MflI MluI MroI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AfaI 114 GTAC GT^AC AhdI 369 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 251 GTGCAC G^TGCAC AlwI 3 GATCC GGATC(4/5) AlwNI 647 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 337 CCTGAGG CC^T[ACGT]AGG ApaLI 251 GTGCAC G^TGCAC AspEI 369 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 135 TGGCCA TGG^CCA BclI 501 TGATCA T^GATCA BglI 683 GCCTCGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 441 GCTGAGC GC^T[ACGT]AGC BpmI 184 CTGGAG CTGGAG(16/14) Bpu1102I 441 GCTGAGC GC^T[ACGT]AGC BsaAI 175 TACGTG [CT]AC^GT[GA] BsaI 478 GAGACC GGTCTC(1/5) BsmFI 128 GTCCC GGGAC(10/14) BspHI 293 TCATGA T^CATGA BsrDI 446 GCAATG GCAATG(2/0) BssSI 685 CTCGTG CACGAG(-5/-1) BstF5I 717 CATCC GGATG(2/0) Bsu36I 337 CCTGAGG CC^T[ACGT]AGG CelII 441 GCTGAGC GC^T[ACGT]AGC EaeI 135 TGGCCA [CT]^GGCC[GA] Eam1105I 369 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 596 CTCTTC CTCTTC(1/4) Eco81I 337 CCTGAGG CC^T[ACGT]AGG FauI 243 CCCGC CCCGC(4/6) FbaI 501 TGATCA T^GATCA FokI 717 CATCC GGATG(9/13) HincII 427 GTCAAC GT[CT]^[GA]AC HindII 427 GTCAAC GT[CT]^[GA]AC HinfI 621 GATTC G^A[ACGT]TC Ksp632I 596 CTCTTC CTCTTC(1/4) MaeII 176 ACGT A^CGT MluNI 135 TGGCCA TGG^CCA MscI 135 TGGCCA TGG^CCA MseI 299 TTAA T^TAA MspA1I 317 CCGCTG C[AC]G^C[GT]G MwoI 683 GCCTCGTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PshAI 651 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 293 TCATGA T^CATGA RsaI 114 GTAC GT^AC SacI 640 GAGCTC GAGCT^C StuI 723 AGGCCT AGG^CCT TfiI 621 GATTC G^A[AT]TC Tru9I 299 TTAA T^TAA XcmI 147 CCATGAATTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AcsI 151 GAATTT [GA]^AATT[CT] 226 AAATTT ApaI 29 GGGCCC GGGCC^C 757 GGGCCC ApoI 151 GAATTT [GA]^AATT[CT] 226 AAATTT BbsI 70 GAAGAC GAAGAC(2/6) 709 GTCTTC BbvI 21 GCTGC GCAGC(8/12) 319 GCTGC BpuAI 70 GAAGAC GAAGAC(2/6) 709 GTCTTC BsiYI 58 CCCTGTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 203 CCTGGCGGTGG BslI 58 CCCTGTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 203 CCTGGCGGTGG Cac8I 453 GCACGC GC[ACGT]^[ACGT]GC 725 GCCTGC EcoT14I 720 CCAAGG C^C[AT][AT]GG 753 CCAAGG Fnu4HI 21 GCTGC GC^[ACGT]GC 319 GCTGC ItaI 21 GCTGC GC^[ACGT]GC 319 GCTGC StyI 720 CCAAGG C^C[AT][AT]GG 753 CCAAGG TseI 21 GCTGC G^C[AT]GC 319 GCTGC Tsp509I 152 AATT ^AATT 227 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AciI AluI AspHI AvaII BanII BmyI BsaJI BsiHKAI BsmAI Bsp1286I BsrI BssKI BstNI Cfr13I DdeI DpnII DraII EcoO109I EcoRII HaeIII HphI MaeIII MboI MboII MnlI MslI MvaI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI Tsp45I TspRI