################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvrII BamHI BanI BbeI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BsiWI BspEI BspHI BspLU11I BsrBI BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I CelII CpoI Eam1105I Eco47III EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI HincII HindII HpaI KasI KpnI KspI MamI MfeI MflI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmeI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI StuI StyI SwaI Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AcsI 19 AAATTT [GA]^AATT[CT] AlwI 1381 GATCC GGATC(4/5) AocI 715 CCTGAGG CC^T[ACGT]AGG ApoI 19 AAATTT [GA]^AATT[CT] AspHI 592 GAGCAC G[AT]GC[AT]^C AviII 653 TGCGCA TGC^GCA BalI 371 TGGCCA TGG^CCA BanII 504 GGGCTC G[GA]GC[CT]^C BbrPI 814 CACGTG CAC^GTG BbsI 1228 GAAGAC GAAGAC(2/6) BbvCI 97 CCTCAGC CCTCAGC(-5/-2) BpmI 1433 CTGGAG CTGGAG(16/14) BpuAI 1228 GAAGAC GAAGAC(2/6) BsaAI 814 CACGTG [CT]AC^GT[GA] BseRI 1436 GAGGAG GAGGAG(10/8) BsgI 5 CTGCAC GTGCAG(16/14) BsiEI 150 CGGCCG CG[GA][CT]^CG BsiHKAI 592 GAGCAC G[AT]GC[AT]^C BsmBI 415 GAGACG CGTCTC(1/5) BsmI 1159 GAATGC GAATGC(1/-1) Bsp1407I 162 TGTACA T^GTACA BspDI 820 ATCGAT AT^CGAT BspMI 650 ACCTGC ACCTGC(4/8) BsrDI 158 GCAATG GCAATG(2/0) BsrFI 152 GCCGGT [GA]^CCGG[CT] BsrGI 162 TGTACA T^GTACA BssHII 567 GCGCGC G^CGCGC BstAPI 105 GCAGCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 218 CCACGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 715 CCTGAGG CC^T[ACGT]AGG Cfr10I 152 GCCGGT [GA]^CCGG[CT] ClaI 820 ATCGAT AT^CGAT DraI 1012 TTTAAA TTT^AAA DraII 92 AGGACCC [GA]G^G[ACGT]CC[CT] DraIII 595 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 45 GACCACATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 275 CCGTGG C^C[GA][CT]GG EagI 150 CGGCCG C^GGCCG EclXI 150 CGGCCG C^GGCCG Eco52I 150 CGGCCG C^GGCCG Eco81I 715 CCTGAGG CC^T[ACGT]AGG EcoO109I 92 AGGACCC [GA]G^G[ACGT]CC[CT] FspI 653 TGCGCA TGC^GCA HaeII 239 GGCGCT [GA]GCGC^[CT] HindIII 1417 AAGCTT A^AGCTT MluNI 371 TGGCCA TGG^CCA MscI 371 TGGCCA TGG^CCA PmaCI 814 CACGTG CAC^GTG PmlI 814 CACGTG CAC^GTG PpuMI 92 AGGACCC [GA]G^G[AT]CC[CT] PstI 1207 CTGCAG CTGCA^G SphI 860 GCATGC GCATG^C SspBI 162 TGTACA T^GTACA Tsp45I 43 GTGAC ^GT[GC]AC TspRI 1068 CAGTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcyI 294 GGCGTC G[GA]^CG[CT]C 517 GACGCC AfaI 163 GTAC GT^AC 767 GTAC BcnI 639 CCGGG CC^[GC]GG 1330 CCGGG BglI 75 GCCCCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 464 GCCCCGAGGGC BsaHI 294 GGCGTC G[GA]^CG[CT]C 517 GACGCC BsaI 574 GAGACC GGTCTC(1/5) 669 GGTCTC EaeI 150 CGGCCG [CT]^GGCC[GA] 371 TGGCCA HgaI 517 GACGC GACGC(5/10) 295 GCGTC Hin1I 294 GGCGTC G[GA]^CG[CT]C 517 GACGCC HinfI 681 GATTC G^A[ACGT]TC 1007 GATTC MaeII 347 ACGT A^CGT 815 ACGT MaeIII 43 GTGAC ^GT[ACGT]AC 353 GTAAC MspA1I 57 CAGCGG C[AC]G^C[GT]G 130 CAGCGG NciI 639 CCGGG CC^[GC]GG 1330 CCGGG NspI 536 GCATGT [GA]CATG^[CT] 860 GCATGC PflMI 299 CCATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1392 CCAGTCTGTGG RsaI 163 GTAC GT^AC 767 GTAC SfaNI 823 GATGC GCATC(5/9) 473 GCATC SfcI 1207 CTGCAG C^T[GA][CT]AG 1281 CTACAG SmlI 232 CTCAAG C^T[CT][GA]AG 1248 CTTGAG TfiI 681 GATTC G^A[AT]TC 1007 GATTC Van91I 299 CCATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1392 CCAGTCTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AccII AciI AluI Asp700I AvaI AvaII BbvI BfaI BmyI BsaJI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI Ksp632I MaeI MboI MboII MnlI MseI MslI MspI MvaI MvnI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TaqI Tru9I TseI Tsp509I TthHB8I XmnI