(Circular) (Six-base) MAPSORT of: pFN21AB5903.seq Check: 8522 from: 1 to: 6208 pFN21AB5903 6208 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4018 278 Size: 53 83 186 936 2482 2468 Fragments arranged by size: 2482 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3551 1937 Size: 1614 4594 Acc65I G'GTAC_C Cuts at: 3529 3529 Size: 6208 AclI AA'CG_TT Cuts at: 1563 4335 4708 1563 Size: 2772 373 3063 Fragments arranged by size: 3063 2772 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2976 3258 4271 5655 5923 1632 Size: 1344 282 1013 1384 268 1917 Fragments arranged by size: 1917 1384 1344 1013 282 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6011 AflIII A'CryG_T Cuts at: 1203 5107 6084 1203 Size: 3904 977 1327 Fragments arranged by size: 3904 1327 977 AgeI A'CCGG_T Cuts at: 5039 5039 Size: 6208 AhdI GACnn_n'nnGTC Cuts at: 4937 4937 Size: 6208 AleI CACnn'nnGTG Cuts at: 1166 2228 1166 Size: 1062 5146 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6208 AlwNI CAG_nnn'CTG Cuts at: 3951 5523 3951 Size: 1572 4636 ApaLI G'TGCA_C Cuts at: 4265 5421 4265 Size: 1156 5052 ApoI r'AATT_y Cuts at: 1493 2024 3517 3743 3895 6004 1493 Size: 531 1493 226 152 2109 1697 Fragments arranged by size: 2109 1697 1493 531 226 152 AseI AT'TA_AT Cuts at: 160 1032 4762 160 Size: 872 3730 1606 Fragments arranged by size: 3730 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 AvaI C'yCGr_G Cuts at: 1870 1943 2068 2328 2471 3533 1870 Size: 73 125 260 143 1062 4545 Fragments arranged by size: 4545 1062 260 143 125 73 BamHI G'GATC_C Cuts at: 3538 3905 3538 Size: 367 5841 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3529 4984 618 Size: 325 217 627 1742 1455 1842 Fragments arranged by size: 1842 1742 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2513 3527 1391 Size: 1122 1014 4072 Fragments arranged by size: 4072 1122 1014 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3240 928 Size: 971 1341 3896 Fragments arranged by size: 3896 1341 971 BbvCI CC'TCA_GC Cuts at: 2103 2103 Size: 6208 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4399 4433 1903 Size: 34 2462 34 3678 Fragments arranged by size: 3678 2462 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4100 5310 4100 Size: 1210 4998 BclI T'GATC_A Cuts at: 1548 1548 Size: 6208 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2086 2475 4819 Size: 107 122 71 631 605 414 389 2344 Cuts at: 4819 136 Size: 1525 Fragments arranged by size: 2344 1525 631 605 414 389 122 107 71 BglII A'GATC_T Cuts at: 6203 6203 Size: 6208 BlpI GC'TnA_GC Cuts at: 3625 3625 Size: 6208 Bme1580I G_kGCm'C Cuts at: 1790 2121 2471 4269 5425 1790 Size: 331 350 1798 1156 2573 Fragments arranged by size: 2573 1798 1156 350 331 BmgBI CAC'GTC Cuts at: 1318 2353 1318 Size: 1035 5173 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2931 3028 3939 Size: 416 53 83 399 24 1514 97 911 Cuts at: 3939 4897 442 Size: 958 1753 Fragments arranged by size: 1753 1514 958 911 416 399 97 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6208 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3459 4868 1239 Size: 2220 1409 2579 Fragments arranged by size: 2579 2220 1409 Bpu10I CC'TnA_GC Cuts at: 2103 2103 Size: 6208 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2222 3274 4333 5198 5496 2222 Size: 1052 1059 865 298 2934 Fragments arranged by size: 2934 1059 1052 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2573 2680 4871 882 Size: 617 1074 107 2191 2219 Fragments arranged by size: 2219 2191 1074 617 107 BsaAI yAC'GTr Cuts at: 493 1204 2821 3999 493 Size: 711 1617 1178 2702 Fragments arranged by size: 2702 1617 1178 711 BsaBI GATnn'nnATC Cuts at: 6202 6202 Size: 6208 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2300 2523 4015 4397 Size: 53 83 186 936 767 223 1492 382 Cuts at: 4397 5032 275 Size: 635 1451 Fragments arranged by size: 1492 1451 936 767 635 382 223 186 83 53 BsaWI w'CCGG_w Cuts at: 4641 5039 5313 5460 5871 4641 Size: 398 274 147 411 4978 Fragments arranged by size: 4978 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2660 2690 1178 Size: 30 165 30 1257 30 4696 Fragments arranged by size: 4696 1257 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3456 1186 Size: 2270 3938 BseYI C'CCAG_C Cuts at: 2559 2856 5411 5907 2559 Size: 297 2555 496 2860 Fragments arranged by size: 2860 2555 496 297 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1995 1163 Size: 573 259 5376 Fragments arranged by size: 5376 573 259 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2158 3658 4419 5447 1540 Size: 275 186 157 1500 761 1028 2301 Fragments arranged by size: 2301 1500 1028 761 275 186 157 BsiHKAI G_wGCw'C Cuts at: 2601 4269 4354 5425 2601 Size: 1668 85 1071 3384 Fragments arranged by size: 3384 1668 1071 85 BsmI GAATG_Cn' Cuts at: 3170 3719 3812 3170 Size: 549 93 5566 Fragments arranged by size: 5566 549 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2414 2414 Size: 6208 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2121 2471 2513 2601 3527 4269 4354 Size: 399 331 350 42 88 926 742 85 Cuts at: 4354 5425 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 926 742 399 350 331 88 85 42 BspEI T'CCGG_A Cuts at: 5871 5871 Size: 6208 BspHI T'CATG_A Cuts at: 4097 5827 4097 Size: 1730 4478 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2664 3563 844 Size: 1820 899 3489 Fragments arranged by size: 3489 1820 899 BsrBI CCG'CTC Cuts at: 1394 1823 4095 1394 Size: 429 2272 3507 Fragments arranged by size: 3507 2272 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2170 4703 4877 65 Size: 1183 922 2533 174 1396 Fragments arranged by size: 2533 1396 1183 922 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2157 4852 5039 1700 Size: 110 347 2695 187 2869 Fragments arranged by size: 2869 2695 347 187 110 BsrGI T'GTAC_A Cuts at: 96 2167 96 Size: 2071 4137 BssHII G'CGCG_C Cuts at: 1925 2572 1925 Size: 647 5561 BssSI C'ACGA_G Cuts at: 4268 5280 4268 Size: 1012 5196 BstAPI GCAn_nnn'nTGC Cuts at: 794 2116 794 Size: 1322 4886 BstBI TT'CG_AA Cuts at: 5012 5012 Size: 6208 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6208 BstXI CCAn_nnnn'nTGG Cuts at: 2230 2230 Size: 6208 BstYI r'GATC_y Cuts at: 1972 3538 3905 4290 4307 5759 5845 5857 6203 Size: 1566 367 385 17 1452 86 12 346 Cuts at: 6203 1972 Size: 1977 Fragments arranged by size: 1977 1566 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2721 2721 Size: 6208 BtgI C'CryG_G Cuts at: 513 1065 2280 513 Size: 552 1215 4441 Fragments arranged by size: 4441 1215 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5469 Fragments arranged by size: 5469 683 56 BtsI GCAGTG_nn' Cuts at: 3724 4518 4538 3724 Size: 794 20 5394 Fragments arranged by size: 5394 794 20 ClaI AT'CG_AT Cuts at: 1554 2826 1554 Size: 1272 4936 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6173 DraI TTT'AAA Cuts at: 3019 3512 3019 Size: 493 5715 DraIII CAC_nnn'GTG Cuts at: 2605 2605 Size: 6208 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2056 5215 809 Size: 507 740 3159 1802 Fragments arranged by size: 3159 1802 740 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2155 2376 3655 Size: 54 1425 50 275 18 325 221 1279 Cuts at: 3655 4544 6173 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1279 889 325 275 221 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2155 3655 1537 Size: 275 343 1500 4090 Fragments arranged by size: 4090 1500 343 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2222 2261 2414 2492 2498 3000 3223 4138 Size: 869 39 153 78 6 502 223 915 Cuts at: 4138 1353 Size: 3423 Fragments arranged by size: 3423 915 869 502 223 153 78 39 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4795 5169 5315 387 Size: 1064 138 3206 374 146 1280 Fragments arranged by size: 3206 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2976 3258 3459 4271 4868 5655 5923 Size: 393 1344 282 201 812 597 787 268 Cuts at: 5923 1239 Size: 1524 Fragments arranged by size: 1524 1344 812 787 597 393 282 268 201 EcoO109I rG'GnC_Cy Cuts at: 2098 2098 Size: 6208 EcoRI G'AATT_C Cuts at: 3517 3895 3517 Size: 378 5830 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3482 3482 Size: 6208 FspI TGC'GCA Cuts at: 2660 4714 2660 Size: 2054 4154 HaeII r_GCGC'y Cuts at: 1678 2248 5355 1678 Size: 570 3107 2531 Fragments arranged by size: 3107 2531 570 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2917 2949 4849 4881 4923 Size: 32 135 32 1345 32 1900 32 42 Cuts at: 4923 4955 1373 Size: 32 2626 Fragments arranged by size: 2626 1900 1345 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3552 3798 669 Size: 988 281 1614 246 3079 Fragments arranged by size: 3079 1614 988 281 246 HindIII A'AGCT_T Cuts at: 748 3422 748 Size: 2674 3534 HpaI GTT'AAC Cuts at: 3798 3798 Size: 6208 KpnI G_GTAC'C Cuts at: 3533 3533 Size: 6208 MfeI C'AATT_G Cuts at: 3807 3807 Size: 6208 MluI A'CGCG_T Cuts at: 6084 6084 Size: 6208 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5322 5506 1305 Size: 4017 184 2007 Fragments arranged by size: 4017 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2378 10 Size: 54 1425 343 546 3840 Fragments arranged by size: 3840 1425 546 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2228 2552 2636 3374 4166 4525 4684 Size: 648 1062 324 84 738 792 359 159 Cuts at: 4684 518 Size: 2042 Fragments arranged by size: 2042 1062 792 738 648 359 324 159 84 MspA1I CmG'CkG Cuts at: 1606 2064 2137 3624 4301 5449 5694 1606 Size: 458 73 1487 677 1148 245 2120 Fragments arranged by size: 2120 1487 1148 677 458 245 73 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5656 NdeI CA'TA_TG Cuts at: 387 387 Size: 6208 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6208 NotI GC'GGCC_GC Cuts at: 3655 3655 Size: 6208 NspI r_CATG'y Cuts at: 1132 1229 2545 2869 3566 5111 1132 Size: 97 1316 324 697 1545 2229 Fragments arranged by size: 2229 1545 1316 697 324 97 PasI CC'CwG_GG Cuts at: 1348 2083 1348 Size: 735 5473 PciI A'CATG_T Cuts at: 5107 5107 Size: 6208 PflMI CCAn_nnn'nTGG Cuts at: 1108 2310 3403 1108 Size: 1202 1093 3913 Fragments arranged by size: 3913 1202 1093 PmeI GTTT'AAAC Cuts at: 3512 3512 Size: 6208 PmlI CAC'GTG Cuts at: 2821 2821 Size: 6208 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2714 2746 3247 3279 4277 4309 5790 5822 2714 Size: 32 501 32 998 32 1481 32 3100 Fragments arranged by size: 3100 1481 998 501 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2098 2098 Size: 6208 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6208 PsiI TTA'TAA Cuts at: 3778 3778 Size: 6208 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2935 2967 2935 Size: 32 6176 PstI C_TGCA'G Cuts at: 830 3216 3560 830 Size: 2386 344 3478 Fragments arranged by size: 3478 2386 344 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6208 SalI G'TCGA_C Cuts at: 1936 3550 1936 Size: 1614 4594 SbfI CC_TGCA'GG Cuts at: 3560 3560 Size: 6208 ScaI AGT'ACT Cuts at: 4456 6168 4456 Size: 1712 4496 SfcI C'TryA_G Cuts at: 826 1045 3212 3286 3556 4691 5372 5563 826 Size: 219 2167 74 270 1135 681 191 1471 Fragments arranged by size: 2167 1471 1135 681 270 219 191 74 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6208 SmaI CCC'GGG Cuts at: 1872 3535 1872 Size: 1663 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2237 3253 4312 5213 5475 820 Size: 197 926 294 1016 1059 901 262 1553 Fragments arranged by size: 1553 1059 1016 926 901 294 262 197 SnaBI TAC'GTA Cuts at: 493 3999 493 Size: 3506 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6208 SphI G_CATG'C Cuts at: 1132 2869 3566 1132 Size: 1737 697 3774 Fragments arranged by size: 3774 1737 697 SspI AAT'ATT Cuts at: 5 52 4132 5 Size: 47 4080 2081 Fragments arranged by size: 4080 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6208 StyI C'CwwG_G Cuts at: 513 1065 3647 513 Size: 552 2582 3074 Fragments arranged by size: 3074 2582 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2302 4235 4252 4405 4590 5035 325 Size: 1255 722 1933 17 153 185 445 1498 Fragments arranged by size: 1933 1498 1255 722 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2167 2771 4454 Size: 274 80 33 51 1443 190 604 1683 Cuts at: 4454 6166 96 Size: 1712 138 Fragments arranged by size: 1712 1683 1443 604 274 190 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2225 2257 2660 2692 6168 6200 2225 Size: 32 403 32 3476 32 2233 Fragments arranged by size: 3476 2233 403 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6208 XbaI T'CTAG_A Cuts at: 3544 3544 Size: 6208 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6208 XmaI C'CCGG_G Cuts at: 1870 3533 1870 Size: 1663 4545 XmnI GAAnn'nnTTC Cuts at: 2255 3096 3237 3325 4337 2255 Size: 841 141 88 1012 4126 Fragments arranged by size: 4126 1012 841 141 88 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4016 276 Size: 53 83 186 936 2482 2468 Fragments arranged by size: 2482 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI