################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BfrI BglII BlnI BsaBI BsaHI BseAI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FseI FspI Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MflI MluI MroI MseI MunI MvnI NarI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI SwaI TaqI Tru9I Tth111I TthHB8I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AgeI 655 ACCGGT A^CCGGT AlwI 715 GATCC GGATC(4/5) AocI 917 CCTCAGG CC^T[ACGT]AGG BanI 1271 GGCACC G^G[CT][GA]CC BbsI 302 GAAGAC GAAGAC(2/6) BclI 395 TGATCA T^GATCA BlpI 261 GCTAAGC GC^T[ACGT]AGC BpmI 460 CTGGAG CTGGAG(16/14) Bpu1102I 261 GCTAAGC GC^T[ACGT]AGC BpuAI 302 GAAGAC GAAGAC(2/6) BsaAI 566 CACGTA [CT]AC^GT[GA] BspHI 41 TCATGA T^CATGA BsrBI 7 CCGCTC CCGCTC(-3/-3) BsrDI 627 CATTGC GCAATG(2/0) Bsu36I 917 CCTCAGG CC^T[ACGT]AGG CelII 261 GCTAAGC GC^T[ACGT]AGC EarI 178 GAAGAG CTCTTC(1/4) Eco81I 917 CCTCAGG CC^T[ACGT]AGG FbaI 395 TGATCA T^GATCA HgaI 1389 GACGC GACGC(5/10) HincII 1027 GTCAAC GT[CT]^[GA]AC HindII 1027 GTCAAC GT[CT]^[GA]AC HphI 468 GGTGA GGTGA(8/7) Ksp632I 178 GAAGAG CTCTTC(1/4) MslI 1422 CACCGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 509 GCCGGC GCC^GGC NdeI 341 CATATG CA^TATG NgoMIV 509 GCCGGC G^CCGGC PinAI 655 ACCGGT A^CCGGT PleI 24 GAGTC GAGTC(4/5) RcaI 41 TCATGA T^CATGA SexAI 1371 ACCAGGT A^CC[AT]GGT SfiI 1104 GGCCCTTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 114 GCATGC GCATG^C TfiI 471 GAATC G^A[AT]TC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 1118 AAATTC [GA]^AATT[CT] 1399 GAATTT ApoI 1118 AAATTC [GA]^AATT[CT] 1399 GAATTT BcnI 779 CCCGG CC^[GC]GG 879 CCGGG BglI 926 GCCTGTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1105 GCCCTTGGGGC BsaWI 409 ACCGGA [AT]^CCGG[AT] 655 ACCGGT BsmBI 12 CGTCTC CGTCTC(1/5) 224 GAGACG BsrFI 509 GCCGGC [GA]^CCGG[CT] 655 ACCGGT BssSI 53 CACGAG CACGAG(-5/-1) 503 CTCGTG BstAPI 588 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 851 GCAGCCAGTGC Cfr10I 509 GCCGGC [GA]^CCGG[CT] 655 ACCGGT DpnII 396 GATC ^GATC 715 GATC DraIII 822 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG 1370 CACCAGGTG HaeII 608 GGCGCT [GA]GCGC^[CT] 839 AGCGCC HinfI 24 GAGTC G^A[ACGT]TC 471 GAATC MboI 396 GATC ^GATC 715 GATC NciI 779 CCCGG CC^[GC]GG 879 CCGGG NcoI 295 CCATGG C^CATGG 806 CCATGG NdeII 396 GATC ^GATC 715 GATC NspI 114 GCATGC [GA]CATG^[CT] 560 ACATGC SacI 527 GAGCTC GAGCT^C 1071 GAGCTC Sau3AI 396 GATC ^GATC 715 GATC StuI 159 AGGCCT AGG^CCT 194 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AciI AfaI AluI AlwNI ApaI AspHI AvaII BalI BanII BbvI BfaI BmyI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DraII DsaI EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MaeI MaeII MaeIII MboII MluNI MnlI MscI MspA1I MspI MvaI MwoI NlaIII NlaIV PflMI PpuMI PstI PvuII RsaI Sau96I ScrFI SfaNI SfcI StyI TseI Tsp45I Tsp509I TspRI Van91I XcmI